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The map label for this gene is entA [C]

Identifier: 41409111

GI number: 41409111

Start: 3351847

End: 3352737

Strand: Reverse

Name: entA [C]

Synonym: MAP3013c

Alternate gene names: 41409111

Gene position: 3352737-3351847 (Counterclockwise)

Preceding gene: 41409114

Following gene: 41409110

Centisome position: 69.42

GC content: 71.38

Gene sequence:

>891_bases
GTGACCATACCGTGGACCCCGGACCGGCTGGGCGACCTCACCGGCAGACGCGTGATCGTGACAGGAGCCACCAACGGCGT
CGGGCTGGGCACCGCCCGTGCCCTGGCCAAGGCCGGCGCCGAGGTGATCCTGGCCGTGCGCAACACCGAACTCGGGAAGC
AACGCGCGGCCCAGATGGGCGGGTCGACCGCCGTGGAAAAGCTCGACCTGGCCGACCTGTCGTCGGTGCGCGCCTTCGCC
GACCGGATCGAGGCGCCGGTGGACATCCTGATCAACAACGCCGGCGCCCTGACCGACCGGCGCACCGAGACGGTCGACGG
CTTCGAGATGACGCTGGGCACCAACCTGCTCGGGCCGTTCGCGCTGACCAACCTGCTGCTGCCCAAGGTGCGCAGCCAGA
TCATTAACGTCGGCTCCGACGCGCACCGCTCGGCGACGCTGCACCTCGACGACCTGCACCTGCGCCGGCACAAGTGGACC
CGGCTGGGCGCCTACGCGCAGTCCAAGCTCGCGGTCATGCTGTGGGGGCTCGAGCTGGACCGCCGGCTGCGCGCGGCGGG
GTCGCCGATCGTGACTCAGCTGACCCACCCGGGTTGGGTGGCGTCGAACCTGTCCAACCTGGGCGACGCGCCGCTGAAGG
CGGTGGCGCACAAGGCGGTCAAAGTGGTGGCCGACCGGCTGGCCAACGACATCGACGAGGGCGCGGCGCCCACCCTGTAC
TGCATCAGCGAGCCGATCCCGCCCGGCAGCTACGTCGGGGTCAGCGGCAGGTTCGGGCTGCGCGGCGGTCCGGTGCTGAT
CGGGCGCACCCCGCTGGCCTGCGATTACGACACGGCCGCGCGACTGGTGGCCTTCGCCGAGCGCGAGACCGGCACGGAAC
TGAGGGTCTAG

Upstream 100 bases:

>100_bases
TGGCGCTGTCAGTCATGTCGAACCGCCTCGGGAGTCGTCGGCTCTTATGTTGGCAGAACCGGTCCAACTTACATAGAACC
GACCATTACTCTGGTTCGCC

Downstream 100 bases:

>100_bases
GGAGGTCGGCCATGGGCGAATTCGACAACACGGTCGCCGTCGTCACCGGCGCCGCGCGCGGGCAGGGCCGCAGCCACGCC
GTCGCGCTGGCGCAGCAGGG

Product: hypothetical protein

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTIPWTPDRLGDLTGRRVIVTGATNGVGLGTARALAKAGAEVILAVRNTELGKQRAAQMGGSTAVEKLDLADLSSVRAFA
DRIEAPVDILINNAGALTDRRTETVDGFEMTLGTNLLGPFALTNLLLPKVRSQIINVGSDAHRSATLHLDDLHLRRHKWT
RLGAYAQSKLAVMLWGLELDRRLRAAGSPIVTQLTHPGWVASNLSNLGDAPLKAVAHKAVKVVADRLANDIDEGAAPTLY
CISEPIPPGSYVGVSGRFGLRGGPVLIGRTPLACDYDTAARLVAFAERETGTELRV

Sequences:

>Translated_296_residues
MTIPWTPDRLGDLTGRRVIVTGATNGVGLGTARALAKAGAEVILAVRNTELGKQRAAQMGGSTAVEKLDLADLSSVRAFA
DRIEAPVDILINNAGALTDRRTETVDGFEMTLGTNLLGPFALTNLLLPKVRSQIINVGSDAHRSATLHLDDLHLRRHKWT
RLGAYAQSKLAVMLWGLELDRRLRAAGSPIVTQLTHPGWVASNLSNLGDAPLKAVAHKAVKVVADRLANDIDEGAAPTLY
CISEPIPPGSYVGVSGRFGLRGGPVLIGRTPLACDYDTAARLVAFAERETGTELRV
>Mature_295_residues
TIPWTPDRLGDLTGRRVIVTGATNGVGLGTARALAKAGAEVILAVRNTELGKQRAAQMGGSTAVEKLDLADLSSVRAFAD
RIEAPVDILINNAGALTDRRTETVDGFEMTLGTNLLGPFALTNLLLPKVRSQIINVGSDAHRSATLHLDDLHLRRHKWTR
LGAYAQSKLAVMLWGLELDRRLRAAGSPIVTQLTHPGWVASNLSNLGDAPLKAVAHKAVKVVADRLANDIDEGAAPTLYC
ISEPIPPGSYVGVSGRFGLRGGPVLIGRTPLACDYDTAARLVAFAERETGTELRV

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI225579078, Length=198, Percent_Identity=44.9494949494949, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI166795268, Length=206, Percent_Identity=43.6893203883495, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI186928839, Length=265, Percent_Identity=36.2264150943396, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI10190746, Length=273, Percent_Identity=34.4322344322344, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI193804850, Length=259, Percent_Identity=34.3629343629344, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI7706523, Length=297, Percent_Identity=31.6498316498317, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI146231950, Length=208, Percent_Identity=41.3461538461538, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI19923931, Length=138, Percent_Identity=48.5507246376812, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558006, Length=206, Percent_Identity=36.4077669902913, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI72001364, Length=254, Percent_Identity=32.2834645669291, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71992402, Length=254, Percent_Identity=30.7086614173228, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17532791, Length=270, Percent_Identity=30.3703703703704, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI193207620, Length=257, Percent_Identity=28.7937743190661, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17532805, Length=255, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535059, Length=252, Percent_Identity=29.3650793650794, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17505416, Length=257, Percent_Identity=28.4046692607004, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI32565532, Length=207, Percent_Identity=30.9178743961353, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921754, Length=206, Percent_Identity=39.8058252427184, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI45554248, Length=215, Percent_Identity=38.1395348837209, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24640117, Length=215, Percent_Identity=38.1395348837209, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20129347, Length=248, Percent_Identity=32.6612903225806, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI45553409, Length=205, Percent_Identity=35.609756097561, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24647948, Length=205, Percent_Identity=35.609756097561, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24647946, Length=205, Percent_Identity=35.609756097561, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28573296, Length=299, Percent_Identity=33.1103678929766, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI22024069, Length=206, Percent_Identity=38.3495145631068, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24586328, Length=203, Percent_Identity=36.4532019704434, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24656154, Length=215, Percent_Identity=33.0232558139535, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922628, Length=215, Percent_Identity=33.0232558139535, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161076371, Length=191, Percent_Identity=37.17277486911, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647931, Length=222, Percent_Identity=33.7837837837838, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 31504; Mature: 31372

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPWTPDRLGDLTGRRVIVTGATNGVGLGTARALAKAGAEVILAVRNTELGKQRAAQMG
CCCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHCC
GSTAVEKLDLADLSSVRAFADRIEAPVDILINNAGALTDRRTETVDGFEMTLGTNLLGPF
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHH
ALTNLLLPKVRSQIINVGSDAHRSATLHLDDLHLRRHKWTRLGAYAQSKLAVMLWGLELD
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCEEEEEECCCHH
RRLRAAGSPIVTQLTHPGWVASNLSNLGDAPLKAVAHKAVKVVADRLANDIDEGAAPTLY
HHHHHCCCCCEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
CISEPIPPGSYVGVSGRFGLRGGPVLIGRTPLACDYDTAARLVAFAERETGTELRV
EECCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TIPWTPDRLGDLTGRRVIVTGATNGVGLGTARALAKAGAEVILAVRNTELGKQRAAQMG
CCCCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHCC
GSTAVEKLDLADLSSVRAFADRIEAPVDILINNAGALTDRRTETVDGFEMTLGTNLLGPF
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHH
ALTNLLLPKVRSQIINVGSDAHRSATLHLDDLHLRRHKWTRLGAYAQSKLAVMLWGLELD
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCEEEEEECCCHH
RRLRAAGSPIVTQLTHPGWVASNLSNLGDAPLKAVAHKAVKVVADRLANDIDEGAAPTLY
HHHHHCCCCCEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
CISEPIPPGSYVGVSGRFGLRGGPVLIGRTPLACDYDTAARLVAFAERETGTELRV
EECCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]