Click here to switch to the map view.

The map label for this gene is amt_2

Identifier: 41409086

GI number: 41409086

Start: 3322878

End: 3324317

Strand: Reverse

Name: amt_2

Synonym: MAP2988c

Alternate gene names: 41409086

Gene position: 3324317-3322878 (Counterclockwise)

Preceding gene: 41409087

Following gene: 41409085

Centisome position: 68.83

GC content: 67.43

Gene sequence:

>1440_bases
TTGCGAGTGACATACCCGATACTGGGCCAGCCCAATACCGGCGATACCGCCTGGATGCTGGCCAGTTCCGCGCTGGTGCT
GTTGATGACGCCGGGTCTGGCGTTTTTCTACGGCGGGATGGTGCGCGCCAGAAGCGTGCTGAACATGCTCATGATGAGCA
TCAGCGCGATGGGCGTGGTCACCGTGCTGTGGGTGCTTTACGGCTATTCGGTCGCCTTCGGCGACGACGTCGGCAACTTC
ATGGGCAAGCCGACGTCCTACTGGGGCCTCAAGGGTCTCATCGGCGTCAACGCGGTGGCCGCCGACCCGAGCAAAGGCAC
TGCGGCAACGGACATTCCGCTGGCCGGCACGCTGCCGGCCACCGTGTTCGTGGCCTTCCAGCTGATGTTCGCGATCATCA
CCGTCGCCCTGATCTCCGGCGCGGTGTCCGACCGGCTGAAGTTCGCCGCCTGGCTGGTGTTCGCCGGCCTGTGGGCGACG
TTCGTCTATTTCCCGGTGGCGCACTGGGTGTTCGCGTTCGACGGCTTCGCCTCCGAGCACGGCGGCTGGATCGCCAACAA
GCTGCACGCGATCGACTTCGCCGGCGGGACGGCGGTGCACATCAATTCCGGTGTGGCGGGCCTGATGCTGGCGATCGTGC
TGGGCAAGCGCCGCGGCTGGCCCACCACGTTGTTCCGGCCGCACAACCTGCCGTTCGTGATGCTGGGCGCCGGGCTGCTG
TGGTTCGGCTGGTACGGGTTCAACGCCGGATCGGCCACCAGCTCCAACGGCGCGGCGGGGTCGACGTTCATGACGACGAC
GATCGCGACCGCCACCGCGATGCTGGCCTGGATGCTCACCGAACGCATCCGCGACGGCAAGGCCACCACGTTGGGCGCGG
CGTCCGGGATCGTCGCCGGGCTGGTCGCCATCACCCCGTCCTGCTCGTCGGTCAACGTGCTGGGCGCGCTGGTGGTGGGC
CTGGTGGCCGGCGTGGTGTGCGCGCTGGCGGTCGGCCTGAAATTCAAGCTGGGCTTTGACGACTCGCTCGACGTGGTCGG
GGTGCACCTGGTCGGCGGGTTGGCCGGCACCCTGCTGGTGGGCCTGCTGGCCGCCCCGGAGAGCCCGGCCATCAGCGGCG
TTACCGGCGTGTCCAAGGGACTGTTCTACGGCGGCGGCTGGGCGCAGCTGGAACGGCAGGCGGTCGGCGCGTTCAGCGTC
CTCATCTACTCTGGGGTGGTTACGCTGATCCTGGCGTTGATCCTGAAGTACACGATGGGGCTTCGTCTCAACCCGGAGGC
CGAAGCCTCGGGTATCGACGAGGCTGAGCACGCCGAGAGTGGTTACGATTTCGCCGTGGCTACCGGCTCGGTTCTCCCGC
CCCGGGTCGCTGTGGCGGATACCCGCAACGGCCTGGAGGAGCAGCGAGTGGGCGACAAAGTGGAGGCAGAGCAGTCATGA

Upstream 100 bases:

>100_bases
AAACACAGCGGCACTATCGCCGAAACAACCATTGCGCAATGTTCTGGATCAGGTCATCGGACGCATGTCTGATGGCCAGA
AGCGGGCCGACCGGAGGAGA

Downstream 100 bases:

>100_bases
AGTTGATCACCGCGATCGTAAAGCCGTTCACGCTCGATGACGTGAAGACCAGCCTCGAGGACGCGGGCGTCCTGGGCATG
ACGGTCAGCGAAATCCAGGG

Product: Amt_2

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MRVTYPILGQPNTGDTAWMLASSALVLLMTPGLAFFYGGMVRARSVLNMLMMSISAMGVVTVLWVLYGYSVAFGDDVGNF
MGKPTSYWGLKGLIGVNAVAADPSKGTAATDIPLAGTLPATVFVAFQLMFAIITVALISGAVSDRLKFAAWLVFAGLWAT
FVYFPVAHWVFAFDGFASEHGGWIANKLHAIDFAGGTAVHINSGVAGLMLAIVLGKRRGWPTTLFRPHNLPFVMLGAGLL
WFGWYGFNAGSATSSNGAAGSTFMTTTIATATAMLAWMLTERIRDGKATTLGAASGIVAGLVAITPSCSSVNVLGALVVG
LVAGVVCALAVGLKFKLGFDDSLDVVGVHLVGGLAGTLLVGLLAAPESPAISGVTGVSKGLFYGGGWAQLERQAVGAFSV
LIYSGVVTLILALILKYTMGLRLNPEAEASGIDEAEHAESGYDFAVATGSVLPPRVAVADTRNGLEEQRVGDKVEAEQS

Sequences:

>Translated_479_residues
MRVTYPILGQPNTGDTAWMLASSALVLLMTPGLAFFYGGMVRARSVLNMLMMSISAMGVVTVLWVLYGYSVAFGDDVGNF
MGKPTSYWGLKGLIGVNAVAADPSKGTAATDIPLAGTLPATVFVAFQLMFAIITVALISGAVSDRLKFAAWLVFAGLWAT
FVYFPVAHWVFAFDGFASEHGGWIANKLHAIDFAGGTAVHINSGVAGLMLAIVLGKRRGWPTTLFRPHNLPFVMLGAGLL
WFGWYGFNAGSATSSNGAAGSTFMTTTIATATAMLAWMLTERIRDGKATTLGAASGIVAGLVAITPSCSSVNVLGALVVG
LVAGVVCALAVGLKFKLGFDDSLDVVGVHLVGGLAGTLLVGLLAAPESPAISGVTGVSKGLFYGGGWAQLERQAVGAFSV
LIYSGVVTLILALILKYTMGLRLNPEAEASGIDEAEHAESGYDFAVATGSVLPPRVAVADTRNGLEEQRVGDKVEAEQS
>Mature_479_residues
MRVTYPILGQPNTGDTAWMLASSALVLLMTPGLAFFYGGMVRARSVLNMLMMSISAMGVVTVLWVLYGYSVAFGDDVGNF
MGKPTSYWGLKGLIGVNAVAADPSKGTAATDIPLAGTLPATVFVAFQLMFAIITVALISGAVSDRLKFAAWLVFAGLWAT
FVYFPVAHWVFAFDGFASEHGGWIANKLHAIDFAGGTAVHINSGVAGLMLAIVLGKRRGWPTTLFRPHNLPFVMLGAGLL
WFGWYGFNAGSATSSNGAAGSTFMTTTIATATAMLAWMLTERIRDGKATTLGAASGIVAGLVAITPSCSSVNVLGALVVG
LVAGVVCALAVGLKFKLGFDDSLDVVGVHLVGGLAGTLLVGLLAAPESPAISGVTGVSKGLFYGGGWAQLERQAVGAFSV
LIYSGVVTLILALILKYTMGLRLNPEAEASGIDEAEHAESGYDFAVATGSVLPPRVAVADTRNGLEEQRVGDKVEAEQS

Specific function: Involved in the uptake of ammonia (Probable) [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=434, Percent_Identity=41.0138248847926, Blast_Score=284, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17550180, Length=450, Percent_Identity=27.3333333333333, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=449, Percent_Identity=28.2850779510022, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17550182, Length=456, Percent_Identity=27.4122807017544, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI133931100, Length=450, Percent_Identity=24.6666666666667, Blast_Score=111, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6325396, Length=475, Percent_Identity=33.4736842105263, Blast_Score=243, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6321559, Length=453, Percent_Identity=33.112582781457, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324187, Length=491, Percent_Identity=33.8085539714868, Blast_Score=229, Evalue=5e-61,
Organism=Drosophila melanogaster, GI161078314, Length=460, Percent_Identity=28.9130434782609, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 49701; Mature: 49701

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTYPILGQPNTGDTAWMLASSALVLLMTPGLAFFYGGMVRARSVLNMLMMSISAMGVV
CEEEECCCCCCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVLWVLYGYSVAFGDDVGNFMGKPTSYWGLKGLIGVNAVAADPSKGTAATDIPLAGTLPA
HHHHHHHCCCCEECCCHHHHCCCCCCCCCCHHHHCCCEEECCCCCCCCCCCCCCCCCHHH
TVFVAFQLMFAIITVALISGAVSDRLKFAAWLVFAGLWATFVYFPVAHWVFAFDGFASEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GGWIANKLHAIDFAGGTAVHINSGVAGLMLAIVLGKRRGWPTTLFRPHNLPFVMLGAGLL
CCCHHHHEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
WFGWYGFNAGSATSSNGAAGSTFMTTTIATATAMLAWMLTERIRDGKATTLGAASGIVAG
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LVAITPSCSSVNVLGALVVGLVAGVVCALAVGLKFKLGFDDSLDVVGVHLVGGLAGTLLV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHH
GLLAAPESPAISGVTGVSKGLFYGGGWAQLERQAVGAFSVLIYSGVVTLILALILKYTMG
HHHCCCCCCCCCCCHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LRLNPEAEASGIDEAEHAESGYDFAVATGSVLPPRVAVADTRNGLEEQRVGDKVEAEQS
CCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCEEECCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRVTYPILGQPNTGDTAWMLASSALVLLMTPGLAFFYGGMVRARSVLNMLMMSISAMGVV
CEEEECCCCCCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVLWVLYGYSVAFGDDVGNFMGKPTSYWGLKGLIGVNAVAADPSKGTAATDIPLAGTLPA
HHHHHHHCCCCEECCCHHHHCCCCCCCCCCHHHHCCCEEECCCCCCCCCCCCCCCCCHHH
TVFVAFQLMFAIITVALISGAVSDRLKFAAWLVFAGLWATFVYFPVAHWVFAFDGFASEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GGWIANKLHAIDFAGGTAVHINSGVAGLMLAIVLGKRRGWPTTLFRPHNLPFVMLGAGLL
CCCHHHHEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
WFGWYGFNAGSATSSNGAAGSTFMTTTIATATAMLAWMLTERIRDGKATTLGAASGIVAG
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LVAITPSCSSVNVLGALVVGLVAGVVCALAVGLKFKLGFDDSLDVVGVHLVGGLAGTLLV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHH
GLLAAPESPAISGVTGVSKGLFYGGGWAQLERQAVGAFSVLIYSGVVTLILALILKYTMG
HHHCCCCCCCCCCCHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LRLNPEAEASGIDEAEHAESGYDFAVATGSVLPPRVAVADTRNGLEEQRVGDKVEAEQS
CCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCEEECCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]