Click here to switch to the map view.

The map label for this gene is cobQ

Identifier: 41409031

GI number: 41409031

Start: 3266886

End: 3268379

Strand: Reverse

Name: cobQ

Synonym: MAP2933c

Alternate gene names: 41409031

Gene position: 3268379-3266886 (Counterclockwise)

Preceding gene: 41409032

Following gene: 41409030

Centisome position: 67.67

GC content: 72.16

Gene sequence:

>1494_bases
GTGAGCGGAGCCCTGCTGGTCGCCGGAACCAGCTCCGACGCCGGGAAGTCGGTGGTGGTGGCCGGCCTGTGCCGGTTGCT
GGCCCGCCGCGGGGTGCGGGTGGCGCCGTTCAAGGCGCAGAACATGTCCAACAACTCGGCGGTCACCGTCGAGGGCGGGG
AAATCGGCCGCGCCCAGGCGATTCAGGCCCGCGCGGCGGGCCTGGAACCCAGCGTGCGGTTCAACCCGATCCTGCTCAAA
CCTGGCAGCGACCGCACCTCGCAGCTGGTGATCAAGGGCCAAGTGGCCGAATCGGTCACCGCGACAAGCTATGTGCAGCA
CCGCGACCGGCTCGCCGCCCTCGTTCTGAACGAATTGACTTGCCTGCGTGACGAATTCGATGCGGTGATCTGCGAGGGGG
CGGGTTCGCCGGCCGAAATCAACCTGCGTGCAACGGATCTAGCCAATATGGGCCTGGCCAGGGCCGCCGATCTCGCGGTG
ATCCTGGTCGGCGACATCGACCGCGGCGGTCTGCTGGCGCACCTGTTCGGCACGGTCGCGGTGCTCGATCCGCAAGACCA
GGCGCTGATCGCCGGGTTCGTCGTCAACAAATTCCGCGGCGACCCCGCGCTGCTGCAGCCCGGATTGCGCCGCCTGCACG
ACATCACGGGCCGGCCCACCTACGGTGTGCTCCCGTACGCCGACCGGCTGTGGCTGGACGCCGAGGACTCGCTGTCGGTG
CTGGCGCACCGCGTCGTCGGCGCGCCCGACCCGCCGCTGGGCGACGACTGGCTGCGGGTGGCCGCCGTCCGGCTGCCCCG
GATCTCCAACTCCACCGACGTCGAGGCGCTGGCCTGCGAACCCGGGGTGCTGGTGCGCTGGGTCAGCGAACCGTCCGACG
TCGCCGACGCCGACCTGGTCGTCATCCCGGGCAGCAAGGCCACCGTCGCCGACCTGTCCTGGCTGCGCGAGCGCGGACTG
GCCGCCGCGATCACCGCGCACGCCGCCGCCGGCAGGCCGGTGCTGGGCATCTGCGGGGGATTTCAGATGCTGTGCCGACG
CATCGACGACCGGGTGGAATCCGGCGCCGGCGAGGTGGCCGGGCTGGGTCTGCTGGACGCCGACATCGCCTTTCATCCCG
CCAAGACGTTGCGCCGCTGGCAGCGGCCGCTGGCCGGCTACGAGATCCACCACGGGCGGGTCTCCCGCTGCGCCGAGCAC
AGCTGGTTCGACTCGGACGCCGAGCCGCAGGGTCTGGTGCGCGGCGCGGTGTTCGGGACGCACTGGCACGGCCTGCTCGA
CAACGACGACTTCCGGCGCGCCTGGCTCGTCCGGGTCGCCGACGCGGCCGGCCGGCGCTTCGTGCCCGGCGACACCGACG
TCGCCGCGCGGCGCGACGCCCAACTCGATCTGGCCGCCGACCTGCTGGCGGCCCACCTCGACGTCGACGCCGTGCTCGGG
TTGCTGGACGGTCCGCCGCCGCGGCCGCGGCTGGCAACTCGGTTGTGCCGGTAG

Upstream 100 bases:

>100_bases
TGGACGGTGGTCACCAAGGACCGCAAGTGGACCGCCCAGTTCGAACACACCCTGCTGGTCACCGACACCGGCGTGGAAAT
CCTGACGCTGCCGTGAGCCG

Downstream 100 bases:

>100_bases
GGAATTCGCGCACCCCGACGGTGAACGCCGTTGTTGACGGCGCGGTCAAAAATCCGCGCCCGCGTATCGGATGCTCGAGA
AGCGTCGCGCACACAGGCCG

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSGALLVAGTSSDAGKSVVVAGLCRLLARRGVRVAPFKAQNMSNNSAVTVEGGEIGRAQAIQARAAGLEPSVRFNPILLK
PGSDRTSQLVIKGQVAESVTATSYVQHRDRLAALVLNELTCLRDEFDAVICEGAGSPAEINLRATDLANMGLARAADLAV
ILVGDIDRGGLLAHLFGTVAVLDPQDQALIAGFVVNKFRGDPALLQPGLRRLHDITGRPTYGVLPYADRLWLDAEDSLSV
LAHRVVGAPDPPLGDDWLRVAAVRLPRISNSTDVEALACEPGVLVRWVSEPSDVADADLVVIPGSKATVADLSWLRERGL
AAAITAHAAAGRPVLGICGGFQMLCRRIDDRVESGAGEVAGLGLLDADIAFHPAKTLRRWQRPLAGYEIHHGRVSRCAEH
SWFDSDAEPQGLVRGAVFGTHWHGLLDNDDFRRAWLVRVADAAGRRFVPGDTDVAARRDAQLDLAADLLAAHLDVDAVLG
LLDGPPPRPRLATRLCR

Sequences:

>Translated_497_residues
MSGALLVAGTSSDAGKSVVVAGLCRLLARRGVRVAPFKAQNMSNNSAVTVEGGEIGRAQAIQARAAGLEPSVRFNPILLK
PGSDRTSQLVIKGQVAESVTATSYVQHRDRLAALVLNELTCLRDEFDAVICEGAGSPAEINLRATDLANMGLARAADLAV
ILVGDIDRGGLLAHLFGTVAVLDPQDQALIAGFVVNKFRGDPALLQPGLRRLHDITGRPTYGVLPYADRLWLDAEDSLSV
LAHRVVGAPDPPLGDDWLRVAAVRLPRISNSTDVEALACEPGVLVRWVSEPSDVADADLVVIPGSKATVADLSWLRERGL
AAAITAHAAAGRPVLGICGGFQMLCRRIDDRVESGAGEVAGLGLLDADIAFHPAKTLRRWQRPLAGYEIHHGRVSRCAEH
SWFDSDAEPQGLVRGAVFGTHWHGLLDNDDFRRAWLVRVADAAGRRFVPGDTDVAARRDAQLDLAADLLAAHLDVDAVLG
LLDGPPPRPRLATRLCR
>Mature_496_residues
SGALLVAGTSSDAGKSVVVAGLCRLLARRGVRVAPFKAQNMSNNSAVTVEGGEIGRAQAIQARAAGLEPSVRFNPILLKP
GSDRTSQLVIKGQVAESVTATSYVQHRDRLAALVLNELTCLRDEFDAVICEGAGSPAEINLRATDLANMGLARAADLAVI
LVGDIDRGGLLAHLFGTVAVLDPQDQALIAGFVVNKFRGDPALLQPGLRRLHDITGRPTYGVLPYADRLWLDAEDSLSVL
AHRVVGAPDPPLGDDWLRVAAVRLPRISNSTDVEALACEPGVLVRWVSEPSDVADADLVVIPGSKATVADLSWLRERGLA
AAITAHAAAGRPVLGICGGFQMLCRRIDDRVESGAGEVAGLGLLDADIAFHPAKTLRRWQRPLAGYEIHHGRVSRCAEHS
WFDSDAEPQGLVRGAVFGTHWHGLLDNDDFRRAWLVRVADAAGRRFVPGDTDVAARRDAQLDLAADLLAAHLDVDAVLGL
LDGPPPRPRLATRLCR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_MYCPA (Q73VS8)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_961867.1
- ProteinModelPortal:   Q73VS8
- EnsemblBacteria:   EBMYCT00000036618
- GeneID:   2717736
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP2933c
- NMPDR:   fig|262316.1.peg.2933
- GeneTree:   EBGT00050000016161
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- ProtClustDB:   PRK00784
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 52913; Mature: 52782

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS51274 GATASE_COBBQ; PS00216 SUGAR_TRANSPORT_1

Important sites: ACT_SITE 338-338 ACT_SITE 423-423

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGALLVAGTSSDAGKSVVVAGLCRLLARRGVRVAPFKAQNMSNNSAVTVEGGEIGRAQA
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEECEECCCCCCCCEEEEECCCCCCHHH
IQARAAGLEPSVRFNPILLKPGSDRTSQLVIKGQVAESVTATSYVQHRDRLAALVLNELT
HHHHHCCCCCCCCCCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLRDEFDAVICEGAGSPAEINLRATDLANMGLARAADLAVILVGDIDRGGLLAHLFGTVA
HHHHHHCEEEECCCCCCCEEEEEEHHHHHCCHHHHCCEEEEEEECCCCCCHHHHHHHHEE
VLDPQDQALIAGFVVNKFRGDPALLQPGLRRLHDITGRPTYGVLPYADRLWLDAEDSLSV
EECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHH
LAHRVVGAPDPPLGDDWLRVAAVRLPRISNSTDVEALACEPGVLVRWVSEPSDVADADLV
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCEE
VIPGSKATVADLSWLRERGLAAAITAHAAAGRPVLGICGGFQMLCRRIDDRVESGAGEVA
EECCCCCHHHHHHHHHHCCCEEEEHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEE
GLGLLDADIAFHPAKTLRRWQRPLAGYEIHHGRVSRCAEHSWFDSDAEPQGLVRGAVFGT
ECEEEECCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHCCCCCCCCCCCHHEEHEEECC
HWHGLLDNDDFRRAWLVRVADAAGRRFVPGDTDVAARRDAQLDLAADLLAAHLDVDAVLG
CCCCCCCCCCHHHHHHEEEHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCHHHHHH
LLDGPPPRPRLATRLCR
HHCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SGALLVAGTSSDAGKSVVVAGLCRLLARRGVRVAPFKAQNMSNNSAVTVEGGEIGRAQA
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEECEECCCCCCCCEEEEECCCCCCHHH
IQARAAGLEPSVRFNPILLKPGSDRTSQLVIKGQVAESVTATSYVQHRDRLAALVLNELT
HHHHHCCCCCCCCCCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLRDEFDAVICEGAGSPAEINLRATDLANMGLARAADLAVILVGDIDRGGLLAHLFGTVA
HHHHHHCEEEECCCCCCCEEEEEEHHHHHCCHHHHCCEEEEEEECCCCCCHHHHHHHHEE
VLDPQDQALIAGFVVNKFRGDPALLQPGLRRLHDITGRPTYGVLPYADRLWLDAEDSLSV
EECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHH
LAHRVVGAPDPPLGDDWLRVAAVRLPRISNSTDVEALACEPGVLVRWVSEPSDVADADLV
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCEE
VIPGSKATVADLSWLRERGLAAAITAHAAAGRPVLGICGGFQMLCRRIDDRVESGAGEVA
EECCCCCHHHHHHHHHHCCCEEEEHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEE
GLGLLDADIAFHPAKTLRRWQRPLAGYEIHHGRVSRCAEHSWFDSDAEPQGLVRGAVFGT
ECEEEECCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHCCCCCCCCCCCHHEEHEEECC
HWHGLLDNDDFRRAWLVRVADAAGRRFVPGDTDVAARRDAQLDLAADLLAAHLDVDAVLG
CCCCCCCCCCHHHHHHEEEHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCHHHHHH
LLDGPPPRPRLATRLCR
HHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA