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The map label for this gene is truB

Identifier: 41408996

GI number: 41408996

Start: 3228359

End: 3229240

Strand: Reverse

Name: truB

Synonym: MAP2898c

Alternate gene names: 41408996

Gene position: 3229240-3228359 (Counterclockwise)

Preceding gene: 41408997

Following gene: 41408995

Centisome position: 66.86

GC content: 74.6

Gene sequence:

>882_bases
ATGAGCCCTCCGGGCCTGGTCGTCGTCGACAAGCCGGCGGGAATGACCAGTCACGACGTGGTGGGCCGGTGTCGCCGGAT
CTTCGCGACCCGACGGGTGGGGCACGCCGGGACGCTCGACCCGATGGCCACCGGCGTGCTGGTGCTCGGCGTCGAGCGCG
CCACCAAGATCCTCGGCCTGCTGACCGCGGCCGCCAAGTCGTACTCGGCCACCATCCGGCTGGGCCAGGCCACCTCCACC
GACGACGCCGAGGGCGACGTGGCGCGCAGCGTCGACGCGCGGCACCTGACGAGCCAGGCGATCGAGGCGGCGGTGGGCGG
GCTGCGCGGCGACATCCACCAGGTGCCCTCGACCGTCAGCGCGATCAAGGTGGCCGGCAAGCGGGCCTACAAGCTGGTCC
GCGAGGGCCAGGCCGTCGAGCTGCCGGCCCGCCCGGTGCGCATCGACCGGTTCGAGGTGCGCGGCCTGCGCGCCGCCGGT
GCGTGCGTGGACGTCGACGTCGAGGTGGACTGCTCGTCGGGAACCTACGTTCGGGCGCTGGCCCGCGACCTGGGCGCGGC
GCTGGGGGTGGGCGGGCATCTGACGGCGCTGCGGCGCACCCGGGTCGGGCGCTTCGGGCTCGAGCAGGCGTATGGGCTCG
ACGAGCTGGCCGAGTGCCCCCGGCTGAGCTACAGCCTGGACGAGGCCTGCCTGCTCATCTTCGGGCGCCGCGATTTGTCC
GCCGACGAGGCCGAGGCCGCCGGCAACGGCCGGGCCCTGGCGGCGGCCGGGATCGACGGAGTGTATGCCGCCTGCGCCCC
CGACGGCCGGGTGATCGCGCTGCTGCGCGACGAGGGCGCGCGGACCAAGTCCGTGGTGGTTTTGCGCCCGGCGACGCTTT
AG

Upstream 100 bases:

>100_bases
GGATCCTGATCGATCCGGCGGCGCTGTGCGGTCCGCCGCTGACCGCGCTGTCGGGCCGGTGGTCGGTGGCGCGCGGGCTG
GTGCTGACCGCCATCGTGTT

Downstream 100 bases:

>100_bases
CCGCCGGGCCCACGCTTTAGCTGCCCCGGCCCAACGGTTGGTCGGGCCACGTTGCACACTGAGAGCCTGAGCCGTTGGGA
AGGGGACAAAGATGTCCAAC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSPPGLVVVDKPAGMTSHDVVGRCRRIFATRRVGHAGTLDPMATGVLVLGVERATKILGLLTAAAKSYSATIRLGQATST
DDAEGDVARSVDARHLTSQAIEAAVGGLRGDIHQVPSTVSAIKVAGKRAYKLVREGQAVELPARPVRIDRFEVRGLRAAG
ACVDVDVEVDCSSGTYVRALARDLGAALGVGGHLTALRRTRVGRFGLEQAYGLDELAECPRLSYSLDEACLLIFGRRDLS
ADEAEAAGNGRALAAAGIDGVYAACAPDGRVIALLRDEGARTKSVVVLRPATL

Sequences:

>Translated_293_residues
MSPPGLVVVDKPAGMTSHDVVGRCRRIFATRRVGHAGTLDPMATGVLVLGVERATKILGLLTAAAKSYSATIRLGQATST
DDAEGDVARSVDARHLTSQAIEAAVGGLRGDIHQVPSTVSAIKVAGKRAYKLVREGQAVELPARPVRIDRFEVRGLRAAG
ACVDVDVEVDCSSGTYVRALARDLGAALGVGGHLTALRRTRVGRFGLEQAYGLDELAECPRLSYSLDEACLLIFGRRDLS
ADEAEAAGNGRALAAAGIDGVYAACAPDGRVIALLRDEGARTKSVVVLRPATL
>Mature_292_residues
SPPGLVVVDKPAGMTSHDVVGRCRRIFATRRVGHAGTLDPMATGVLVLGVERATKILGLLTAAAKSYSATIRLGQATSTD
DAEGDVARSVDARHLTSQAIEAAVGGLRGDIHQVPSTVSAIKVAGKRAYKLVREGQAVELPARPVRIDRFEVRGLRAAGA
CVDVDVEVDCSSGTYVRALARDLGAALGVGGHLTALRRTRVGRFGLEQAYGLDELAECPRLSYSLDEACLLIFGRRDLSA
DEAEAAGNGRALAAAGIDGVYAACAPDGRVIALLRDEGARTKSVVVLRPATL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=241, Percent_Identity=32.3651452282158, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4503337, Length=200, Percent_Identity=32.5, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI215599015, Length=200, Percent_Identity=33.5, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI2367200, Length=216, Percent_Identity=39.3518518518519, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17553978, Length=200, Percent_Identity=31.5, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=199, Percent_Identity=32.1608040201005, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324037, Length=133, Percent_Identity=29.3233082706767, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364189, Length=199, Percent_Identity=32.1608040201005, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364187, Length=199, Percent_Identity=32.1608040201005, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364185, Length=199, Percent_Identity=32.1608040201005, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364183, Length=199, Percent_Identity=32.1608040201005, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI62471759, Length=199, Percent_Identity=32.1608040201005, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17975520, Length=199, Percent_Identity=32.1608040201005, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR015225
- InterPro:   IPR014780 [H]

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 30590; Mature: 30459

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPGLVVVDKPAGMTSHDVVGRCRRIFATRRVGHAGTLDPMATGVLVLGVERATKILGL
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCEEEEEHHHHHHHHHH
LTAAAKSYSATIRLGQATSTDDAEGDVARSVDARHLTSQAIEAAVGGLRGDIHQVPSTVS
HHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AIKVAGKRAYKLVREGQAVELPARPVRIDRFEVRGLRAAGACVDVDVEVDCSSGTYVRAL
HHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCEEECCCEEEEEEEEECCCCHHHHHH
ARDLGAALGVGGHLTALRRTRVGRFGLEQAYGLDELAECPRLSYSLDEACLLIFGRRDLS
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHCCCCCCCCCCEEEEEECCCCCC
ADEAEAAGNGRALAAAGIDGVYAACAPDGRVIALLRDEGARTKSVVVLRPATL
CCHHHHCCCCCEEEECCCCCEEEEECCCCCEEEEEECCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SPPGLVVVDKPAGMTSHDVVGRCRRIFATRRVGHAGTLDPMATGVLVLGVERATKILGL
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCEEEEEHHHHHHHHHH
LTAAAKSYSATIRLGQATSTDDAEGDVARSVDARHLTSQAIEAAVGGLRGDIHQVPSTVS
HHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AIKVAGKRAYKLVREGQAVELPARPVRIDRFEVRGLRAAGACVDVDVEVDCSSGTYVRAL
HHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCEEECCCEEEEEEEEECCCCHHHHHH
ARDLGAALGVGGHLTALRRTRVGRFGLEQAYGLDELAECPRLSYSLDEACLLIFGRRDLS
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHCCCCCCCCCCEEEEEECCCCCC
ADEAEAAGNGRALAAAGIDGVYAACAPDGRVIALLRDEGARTKSVVVLRPATL
CCHHHHCCCCCEEEECCCCCEEEEECCCCCEEEEEECCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA