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The map label for this gene is fadE21

Identifier: 41408994

GI number: 41408994

Start: 3225813

End: 3227069

Strand: Reverse

Name: fadE21

Synonym: MAP2896c

Alternate gene names: 41408994

Gene position: 3227069-3225813 (Counterclockwise)

Preceding gene: 41408995

Following gene: 41408991

Centisome position: 66.82

GC content: 64.76

Gene sequence:

>1257_bases
ATGATCGAATGGTCCGACACCGACCTGATGGTCCGCGACGCCGTCCGGCAATTCGTGGACAAGGAGATCCGGCCGCACCT
GGACGAATTGGAAAGCGGCGCGATGTCGCCCTATCCGATCGCGCGCAAGCTGTTCAGCCAGTTCGGCCTCGACACCATGG
CCGCCGAGGCGGTCAAGAAGGTGCTGGACCGCGAGCGTAGCGGAGTCGACGAAAATGGCGACAGCTCAGGCGCCGCAAAT
GAATTCAGCGGCGGCGGCGCGCAAGGCTCGATGATCGCGGTGCTGGTCTCCGAGATCGCTCGGGTCAGCATCGGATTGCT
GTCCACCGCGTCGGTCAGCCTGGGCCTGGGCGCGGCCACCATCGCCAGCCGCGGGACGCTGGCCCAAAAAGAGCGCTGGC
TGCCCGAGCTGATGACGCTGGAAAAGATTGCGGCGTGGGCGATCACGGAGCCGGACTCGGGCTCGGATGCGTTCGGCGGC
ATGAAGACCTCAGTCCGGCGCTCCGGCGACGGAGATGGGGCCTACATCCTCAACGGGCAGAAGACCTTCATCACCAACGG
GCCCTACGCCGACGTGCTGGTGGTCTACGCCAAACTCGACGAGGGCGACCCGTCGGTGGACAAGCGCAACCGCCCGGTGC
TGGTCTTCGTGCTCGATGCGGGCATGCCGGGCCTAACCCAGGGCAAGCCGTTCAAGAAGATGGGCATGATGTCCTCGCCG
ACGGGCGAGCTGTTCTTCGACAACGTGCGGCTGACCCCGGATCGTCTGCTCGGCGAGAGCGAACAGCACACCGAAGGCGA
CGGCCGCGACAGCGCCCGGGCCAATTTCGCCGCCGAGCGGATCGGGATCGCGATGATGGCGCTGGGCATCATCGACGAAT
GCCACCGACTGTGCGTCGATTACGCCAATAGCAGGACATTGTGGGGCAAGAACATTGGGCAGTTCCAGCTGATCCAGCTC
AAGCTGGCCAAGATGGAGATCGCCCGAATGAACGTGCAGAACATGGTCTTTCACACCATCGAACGCCAGCGGGCGGGTAA
ACCGCTGACGCTGGCCGAGGCCTCGGCAATCAAGCTGTACTCGTCGGAGGCGGCCACCGAGGTGGCGATGGAGGCCGTGC
AGCTGTTCGGCGGCAACGGCTACATGGCCGAGTACCGGGTGGAGCAGCTGGCCCGCGACGCCAAATCGCTGATGATCTAC
GCCGGCAGCAACGAGGTCCAGGTCACCCACATCGCGAAGGGTCTGCTGTCGAGCTGA

Upstream 100 bases:

>100_bases
CGTCAGGTCGAAAACGTCAGCGCCGCACTGCAACACAACATCGGGCTGGGCGGCGCGGCCGTCGTCACCGCCTACCAGCG
CGCCGAACGCTAAGCGCGCC

Downstream 100 bases:

>100_bases
GTCGGTCGCGCCGACCTGGTCGCCGAACGTGCGCGGCGCGCGAAAAAACGGCGGCGGAATCGCAACCAGTGCACGCTCGG
CGGGGGGAGAGGCGCGGCGC

Product: FadE21

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MIEWSDTDLMVRDAVRQFVDKEIRPHLDELESGAMSPYPIARKLFSQFGLDTMAAEAVKKVLDRERSGVDENGDSSGAAN
EFSGGGAQGSMIAVLVSEIARVSIGLLSTASVSLGLGAATIASRGTLAQKERWLPELMTLEKIAAWAITEPDSGSDAFGG
MKTSVRRSGDGDGAYILNGQKTFITNGPYADVLVVYAKLDEGDPSVDKRNRPVLVFVLDAGMPGLTQGKPFKKMGMMSSP
TGELFFDNVRLTPDRLLGESEQHTEGDGRDSARANFAAERIGIAMMALGIIDECHRLCVDYANSRTLWGKNIGQFQLIQL
KLAKMEIARMNVQNMVFHTIERQRAGKPLTLAEASAIKLYSSEAATEVAMEAVQLFGGNGYMAEYRVEQLARDAKSLMIY
AGSNEVQVTHIAKGLLSS

Sequences:

>Translated_418_residues
MIEWSDTDLMVRDAVRQFVDKEIRPHLDELESGAMSPYPIARKLFSQFGLDTMAAEAVKKVLDRERSGVDENGDSSGAAN
EFSGGGAQGSMIAVLVSEIARVSIGLLSTASVSLGLGAATIASRGTLAQKERWLPELMTLEKIAAWAITEPDSGSDAFGG
MKTSVRRSGDGDGAYILNGQKTFITNGPYADVLVVYAKLDEGDPSVDKRNRPVLVFVLDAGMPGLTQGKPFKKMGMMSSP
TGELFFDNVRLTPDRLLGESEQHTEGDGRDSARANFAAERIGIAMMALGIIDECHRLCVDYANSRTLWGKNIGQFQLIQL
KLAKMEIARMNVQNMVFHTIERQRAGKPLTLAEASAIKLYSSEAATEVAMEAVQLFGGNGYMAEYRVEQLARDAKSLMIY
AGSNEVQVTHIAKGLLSS
>Mature_418_residues
MIEWSDTDLMVRDAVRQFVDKEIRPHLDELESGAMSPYPIARKLFSQFGLDTMAAEAVKKVLDRERSGVDENGDSSGAAN
EFSGGGAQGSMIAVLVSEIARVSIGLLSTASVSLGLGAATIASRGTLAQKERWLPELMTLEKIAAWAITEPDSGSDAFGG
MKTSVRRSGDGDGAYILNGQKTFITNGPYADVLVVYAKLDEGDPSVDKRNRPVLVFVLDAGMPGLTQGKPFKKMGMMSSP
TGELFFDNVRLTPDRLLGESEQHTEGDGRDSARANFAAERIGIAMMALGIIDECHRLCVDYANSRTLWGKNIGQFQLIQL
KLAKMEIARMNVQNMVFHTIERQRAGKPLTLAEASAIKLYSSEAATEVAMEAVQLFGGNGYMAEYRVEQLARDAKSLMIY
AGSNEVQVTHIAKGLLSS

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958414, Length=327, Percent_Identity=36.085626911315, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI226958412, Length=327, Percent_Identity=36.085626911315, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4557235, Length=327, Percent_Identity=35.7798165137615, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI76496475, Length=327, Percent_Identity=35.7798165137615, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI4557233, Length=419, Percent_Identity=31.7422434367542, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI4501859, Length=416, Percent_Identity=29.8076923076923, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI21361497, Length=335, Percent_Identity=33.134328358209, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4501857, Length=408, Percent_Identity=28.921568627451, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI7656849, Length=314, Percent_Identity=32.484076433121, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI4557231, Length=416, Percent_Identity=28.8461538461538, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI187960098, Length=416, Percent_Identity=28.8461538461538, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI4503943, Length=339, Percent_Identity=28.3185840707965, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI7669494, Length=324, Percent_Identity=29.320987654321, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI48976061, Length=298, Percent_Identity=27.1812080536913, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI211971008, Length=298, Percent_Identity=27.1812080536913, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI38505218, Length=297, Percent_Identity=22.5589225589226, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786223, Length=301, Percent_Identity=30.8970099667774, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081958, Length=272, Percent_Identity=33.4558823529412, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17538396, Length=417, Percent_Identity=31.1750599520384, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71985184, Length=334, Percent_Identity=32.6347305389222, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17508101, Length=348, Percent_Identity=31.6091954022989, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17570075, Length=406, Percent_Identity=30.2955665024631, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17506239, Length=415, Percent_Identity=28.433734939759, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17569725, Length=416, Percent_Identity=29.0865384615385, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17534899, Length=417, Percent_Identity=27.3381294964029, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17534353, Length=339, Percent_Identity=30.9734513274336, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17533517, Length=409, Percent_Identity=26.161369193154, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71985192, Length=247, Percent_Identity=34.0080971659919, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71990804, Length=282, Percent_Identity=31.5602836879433, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17505929, Length=411, Percent_Identity=25.5474452554745, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17551932, Length=410, Percent_Identity=24.6341463414634, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32563615, Length=238, Percent_Identity=30.2521008403361, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71982178, Length=235, Percent_Identity=27.6595744680851, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531909, Length=254, Percent_Identity=27.9527559055118, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI86563381, Length=287, Percent_Identity=26.8292682926829, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI86563383, Length=287, Percent_Identity=26.8292682926829, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562446, Length=298, Percent_Identity=26.8456375838926, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI281363737, Length=403, Percent_Identity=30.7692307692308, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI21355753, Length=333, Percent_Identity=31.8318318318318, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24646207, Length=421, Percent_Identity=30.166270783848, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21356377, Length=414, Percent_Identity=29.7101449275362, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24660351, Length=418, Percent_Identity=28.2296650717703, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24666513, Length=415, Percent_Identity=27.710843373494, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI19920834, Length=420, Percent_Identity=26.1904761904762, Blast_Score=118, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 45273; Mature: 45273

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00072 ACYL_COA_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEWSDTDLMVRDAVRQFVDKEIRPHLDELESGAMSPYPIARKLFSQFGLDTMAAEAVKK
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
VLDRERSGVDENGDSSGAANEFSGGGAQGSMIAVLVSEIARVSIGLLSTASVSLGLGAAT
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IASRGTLAQKERWLPELMTLEKIAAWAITEPDSGSDAFGGMKTSVRRSGDGDGAYILNGQ
HHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHCCCCCCCEEEECCC
KTFITNGPYADVLVVYAKLDEGDPSVDKRNRPVLVFVLDAGMPGLTQGKPFKKMGMMSSP
EEEEECCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCC
TGELFFDNVRLTPDRLLGESEQHTEGDGRDSARANFAAERIGIAMMALGIIDECHRLCVD
CCCEEECCEEECHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YANSRTLWGKNIGQFQLIQLKLAKMEIARMNVQNMVFHTIERQRAGKPLTLAEASAIKLY
HCCCCEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHEEH
SSEAATEVAMEAVQLFGGNGYMAEYRVEQLARDAKSLMIYAGSNEVQVTHIAKGLLSS
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEHHHHHHHHHCC
>Mature Secondary Structure
MIEWSDTDLMVRDAVRQFVDKEIRPHLDELESGAMSPYPIARKLFSQFGLDTMAAEAVKK
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
VLDRERSGVDENGDSSGAANEFSGGGAQGSMIAVLVSEIARVSIGLLSTASVSLGLGAAT
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IASRGTLAQKERWLPELMTLEKIAAWAITEPDSGSDAFGGMKTSVRRSGDGDGAYILNGQ
HHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHCCCCCCCEEEECCC
KTFITNGPYADVLVVYAKLDEGDPSVDKRNRPVLVFVLDAGMPGLTQGKPFKKMGMMSSP
EEEEECCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCC
TGELFFDNVRLTPDRLLGESEQHTEGDGRDSARANFAAERIGIAMMALGIIDECHRLCVD
CCCEEECCEEECHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YANSRTLWGKNIGQFQLIQLKLAKMEIARMNVQNMVFHTIERQRAGKPLTLAEASAIKLY
HCCCCEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHEEH
SSEAATEVAMEAVQLFGGNGYMAEYRVEQLARDAKSLMIYAGSNEVQVTHIAKGLLSS
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759838; 8969508; 9384377 [H]