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The map label for this gene is sigA

Identifier: 41408918

GI number: 41408918

Start: 3148633

End: 3150162

Strand: Direct

Name: sigA

Synonym: MAP2820

Alternate gene names: 41408918

Gene position: 3148633-3150162 (Clockwise)

Preceding gene: 41408917

Following gene: 41408919

Centisome position: 65.19

GC content: 69.22

Gene sequence:

>1530_bases
GTGGCAGCGACCAAAGCAAGCCCGGCGACGGATGGGCCGGTGAAACGCACCGCCACAAAGTCTCCGTCGTCCCCCGCCAA
GAGGCCGGCGGCCAAGGCCGCCAATGGCTCCGCGCCCGCCAAGCGGGCCACCAAGACCGCATCGCGGTCCGCCAAGTCCG
AGGCCGGCGCGGCGGCCGAACCCGCCAAGAAGACCCGCTCGTCGGCCAAGGGTGCCGACGCCAAGGCGCCGTCGGCGCGC
GGGACGAAGGCGGCCAAGGGCGGCCCGGCCGATCCCGACGCCCTCGACACCGACGGCGCCGTCGAGGATCTCGACACCGA
ACCCGACCTCGAGGGCGAGCCCGGCGAGGACCTCGACATCGACACCGACCTGAACCTCGACGACCTCGAGGAGGACGTGG
CGGCCGACGACGCCGACATCGAGCCCGGTGACGCCGAGGAGGGCGAGGACGAGGAAGCCGCCGCCCCCAAGGCTGCCGGC
GCCACGGTCGCCGACGAGGACGACGAGATCGCCGAGCCGTCCGAAAAGGACAAGGCCTCCGGCGATTTCGTCTGGGACGA
GGACGAATCCGAGGCGTTGCGGCAGGCCCGCAGGGACGCCGAGCTGACCGCGTCGGCCGACTCGGTGCGCGCCTACCTCA
AGCAGATCGGTAAGGTCGCGCTGCTCAACGCCGAGGAAGAGGTCGAGCTGGCCAAGCGGATCGAGGCCGGCCTGTACGCC
ACCCAGCTGATGTCGGAGATGGCCGAGCGCGGCGAGAAGCTGCCCGCCGCCCAGCGCCGCGACATGATGTGGATCTGCCG
CGACGGCGACCGCGCGAAAAACCATCTGCTGGAAGCGAATCTGCGGTTGGTGGTGTCGCTGGCCAAGCGCTACACAGGCC
GCGGGATGGCGTTCCTCGATTTGATCCAGGAGGGCAACCTGGGCCTGATCCGTGCGGTCGAGAAGTTCGACTACACCAAG
GGCTACAAGTTCTCCACCTACGCGACGTGGTGGATCCGTCAGGCCATCACCCGGGCCATGGCCGACCAGGCCCGCACCAT
CCGCATCCCGGTGCACATGGTCGAGGTGATCAACAAGCTGGGCCGCATTCAGCGCGAGCTGCTGCAGGACCCGGGCCGCG
AGCCCACGCCCGAGGAGCTGGCCAAGGAAATGGACATCACGCCCGAAAAGGTGCTCGAGATCCAGCAATACGCGCGCGAG
CCGATCTCGCTGGACCAGACGATCGGCGACGAGGGCGATTCCCAGCTGGGCGACTTCATCGAGGACAGCGAGGCCGTGGT
GGCCGTCGACGCGGTGTCGTTCACGCTGCTGCAGGACCAGCTGCAGTCGGTGCTGGAGACGCTGTCGGAGCGCGAGGCCG
GCGTGGTGCGGCTGCGGTTCGGCCTGACCGACGGCCAGCCCCGCACCCTGGACGAGATCGGCCAGGTCTACGGGGTGACC
CGCGAGCGCATCCGCCAGATCGAGTCCAAGACGATGTCAAAGTTGCGCCACCCCAGCCGTTCCCAGGTGCTGCGCGACTA
CCTGGACTGA

Upstream 100 bases:

>100_bases
ACTGGTCACGCTGATACCAACGCCGACATCGACATAGCAGACACTTTCGGTTAACCATGCCCACTACCCACCGAAAGTGA
GCCCAAACGAAGGGGTGTAA

Downstream 100 bases:

>100_bases
GCGGTCCGGGCGGAACGCACCGGAAGCGTTGCCGCCCAAGGCAATTCAGCCAGGTGTGAAAAATGCGTGGCCCCGGCGGT
GTCGCCGACGTACCGTGAGG

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MAATKASPATDGPVKRTATKSPSSPAKRPAAKAANGSAPAKRATKTASRSAKSEAGAAAEPAKKTRSSAKGADAKAPSAR
GTKAAKGGPADPDALDTDGAVEDLDTEPDLEGEPGEDLDIDTDLNLDDLEEDVAADDADIEPGDAEEGEDEEAAAPKAAG
ATVADEDDEIAEPSEKDKASGDFVWDEDESEALRQARRDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYA
TQLMSEMAERGEKLPAAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTK
GYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDPGREPTPEELAKEMDITPEKVLEIQQYARE
PISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVT
RERIRQIESKTMSKLRHPSRSQVLRDYLD

Sequences:

>Translated_509_residues
MAATKASPATDGPVKRTATKSPSSPAKRPAAKAANGSAPAKRATKTASRSAKSEAGAAAEPAKKTRSSAKGADAKAPSAR
GTKAAKGGPADPDALDTDGAVEDLDTEPDLEGEPGEDLDIDTDLNLDDLEEDVAADDADIEPGDAEEGEDEEAAAPKAAG
ATVADEDDEIAEPSEKDKASGDFVWDEDESEALRQARRDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYA
TQLMSEMAERGEKLPAAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTK
GYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDPGREPTPEELAKEMDITPEKVLEIQQYARE
PISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVT
RERIRQIESKTMSKLRHPSRSQVLRDYLD
>Mature_508_residues
AATKASPATDGPVKRTATKSPSSPAKRPAAKAANGSAPAKRATKTASRSAKSEAGAAAEPAKKTRSSAKGADAKAPSARG
TKAAKGGPADPDALDTDGAVEDLDTEPDLEGEPGEDLDIDTDLNLDDLEEDVAADDADIEPGDAEEGEDEEAAAPKAAGA
TVADEDDEIAEPSEKDKASGDFVWDEDESEALRQARRDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYAT
QLMSEMAERGEKLPAAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKG
YKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDPGREPTPEELAKEMDITPEKVLEIQQYAREP
ISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTR
ERIRQIESKTMSKLRHPSRSQVLRDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=239, Percent_Identity=60.2510460251046, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1789098, Length=346, Percent_Identity=37.5722543352601, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789871, Length=231, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 55405; Mature: 55274

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATKASPATDGPVKRTATKSPSSPAKRPAAKAANGSAPAKRATKTASRSAKSEAGAAAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
PAKKTRSSAKGADAKAPSARGTKAAKGGPADPDALDTDGAVEDLDTEPDLEGEPGEDLDI
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC
DTDLNLDDLEEDVAADDADIEPGDAEEGEDEEAAAPKAAGATVADEDDEIAEPSEKDKAS
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHCCCCCCCCCC
GDFVWDEDESEALRQARRDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYA
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHH
TQLMSEMAERGEKLPAAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLD
HHHHHHHHHHHHCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKL
HHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHH
GRIQRELLQDPGREPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQLGDFI
HHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHH
EDSEAVVAVDAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVT
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHH
RERIRQIESKTMSKLRHPSRSQVLRDYLD
HHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure 
AATKASPATDGPVKRTATKSPSSPAKRPAAKAANGSAPAKRATKTASRSAKSEAGAAAE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
PAKKTRSSAKGADAKAPSARGTKAAKGGPADPDALDTDGAVEDLDTEPDLEGEPGEDLDI
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC
DTDLNLDDLEEDVAADDADIEPGDAEEGEDEEAAAPKAAGATVADEDDEIAEPSEKDKAS
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHCCCCCCCCCC
GDFVWDEDESEALRQARRDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYA
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHH
TQLMSEMAERGEKLPAAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLD
HHHHHHHHHHHHCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKL
HHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHH
GRIQRELLQDPGREPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQLGDFI
HHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHH
EDSEAVVAVDAVSFTLLQDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVT
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHH
RERIRQIESKTMSKLRHPSRSQVLRDYLD
HHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]