| Definition | Mycobacterium avium subsp. paratuberculosis K-10, complete genome. |
|---|---|
| Accession | NC_002944 |
| Length | 4,829,781 |
Click here to switch to the map view.
The map label for this gene is cysD
Identifier: 41408583
GI number: 41408583
Start: 2789266
End: 2790210
Strand: Reverse
Name: cysD
Synonym: MAP2485c
Alternate gene names: 41408583
Gene position: 2790210-2789266 (Counterclockwise)
Preceding gene: 41408585
Following gene: 41408582
Centisome position: 57.77
GC content: 67.83
Gene sequence:
>945_bases TTGCCGAAGGAAGCCATGACCGCTGATCTGCAGACGGCGCCCGCCGCCGGACAGTACGAATTGAGCCATCTGCGCTCGCT GGAGGCGGAGGCGATCCACATCATCCGTGAGGTCGCCGCCGAGTTCGAGCGCCCGGTACTGCTGTTCTCCGGCGGCAAGG ACTCGATCGTCATGCTGCACCTGGCGTTGCGGGCCTTCCGGCCCGGACGGCTGCCGTTCCCGGTGATGCACGTCGACACC GGGCACAACTTCGACGAGGTGATCGCCACCCGCGACGAGTTGGTCAAGCAGCACGGCCTGCGCTTGGTGGTCGCCTCGGT GCAGGACGACATCGACGCCGGACGGGTGGTCGAGCCGAAGCAGGGACGCAACCCGATCCAGACCGTGACCCTGCTGCGCG CCATCCGCGAGAACAAGTTCGACGCCGCGTTCGGCGGGGCGCGCCGCGACGAGGAGAAGGCCCGCGCCAAGGAGCGGGTG TTCAGCTTCCGCGACGAGTTCGGGCAGTGGGATCCGAAGGCGCAGCGGCCCGAGCTGTGGAACCTCTACAACGGCCGGCA CCACAAGGGCGAGCACATCCGGGTGTTCCCGCTGTCCAACTGGACCGAATTCGACATCTGGTCCTACATCGGCGCCGAGA ACATCGCGCTGCCGTCCATCTATTACGCCCACCGGCGCAAGGTGTTTCGCCGCGACGGCATGCTGCTCGCGGTCGACCGG AACATGCAACCCAATCCCGACGAGCCGGTGTTCGAGGCGACGGTGCGGTTCCGCACCGTCGGGGACGTCACCTGCACCGG CTGTGTGGAATCGCAGGCGGCCACGGTGGACGAGGTCATTGCCGAGACCGCGGTGTCCCGGCTCACCGAGCGCGGGGCGA CCCGGGCGGACGACCGGATCTCGGAGGCCGGCATGGAAGACCGCAAGCGGCAGGGGTACTTCTGA
Upstream 100 bases:
>100_bases GACGCCGCCCGCGCCGGAGTTGACAGCGCCGGCGCCGGCTGGTCTCATAGATCGTTAAGATGCTAAATATGGTCAGTTCT GCCAGGTTTTATCTCCGACC
Downstream 100 bases:
>100_bases TGGCCGCCCCAACCACGCTATTGCGTTTGGCCACTGCGGGATCCGTCGACGACGGTAAGTCCACGCTGATCGGCCGGCTG CTCTACGACTCCAAGGCCGT
Product: sulfate adenylyltransferase subunit 2
Products: NA
Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]
Number of amino acids: Translated: 314; Mature: 313
Protein sequence:
>314_residues MPKEAMTADLQTAPAAGQYELSHLRSLEAEAIHIIREVAAEFERPVLLFSGGKDSIVMLHLALRAFRPGRLPFPVMHVDT GHNFDEVIATRDELVKQHGLRLVVASVQDDIDAGRVVEPKQGRNPIQTVTLLRAIRENKFDAAFGGARRDEEKARAKERV FSFRDEFGQWDPKAQRPELWNLYNGRHHKGEHIRVFPLSNWTEFDIWSYIGAENIALPSIYYAHRRKVFRRDGMLLAVDR NMQPNPDEPVFEATVRFRTVGDVTCTGCVESQAATVDEVIAETAVSRLTERGATRADDRISEAGMEDRKRQGYF
Sequences:
>Translated_314_residues MPKEAMTADLQTAPAAGQYELSHLRSLEAEAIHIIREVAAEFERPVLLFSGGKDSIVMLHLALRAFRPGRLPFPVMHVDT GHNFDEVIATRDELVKQHGLRLVVASVQDDIDAGRVVEPKQGRNPIQTVTLLRAIRENKFDAAFGGARRDEEKARAKERV FSFRDEFGQWDPKAQRPELWNLYNGRHHKGEHIRVFPLSNWTEFDIWSYIGAENIALPSIYYAHRRKVFRRDGMLLAVDR NMQPNPDEPVFEATVRFRTVGDVTCTGCVESQAATVDEVIAETAVSRLTERGATRADDRISEAGMEDRKRQGYF >Mature_313_residues PKEAMTADLQTAPAAGQYELSHLRSLEAEAIHIIREVAAEFERPVLLFSGGKDSIVMLHLALRAFRPGRLPFPVMHVDTG HNFDEVIATRDELVKQHGLRLVVASVQDDIDAGRVVEPKQGRNPIQTVTLLRAIRENKFDAAFGGARRDEEKARAKERVF SFRDEFGQWDPKAQRPELWNLYNGRHHKGEHIRVFPLSNWTEFDIWSYIGAENIALPSIYYAHRRKVFRRDGMLLAVDRN MQPNPDEPVFEATVRFRTVGDVTCTGCVESQAATVDEVIAETAVSRLTERGATRADDRISEAGMEDRKRQGYF
Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]
COG id: COG0175
COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]
Homologues:
Organism=Escherichia coli, GI1789109, Length=303, Percent_Identity=53.7953795379538, Blast_Score=304, Evalue=4e-84,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002500 - InterPro: IPR014729 - InterPro: IPR011784 [H]
Pfam domain/function: PF01507 PAPS_reduct [H]
EC number: =2.7.7.4 [H]
Molecular weight: Translated: 35667; Mature: 35536
Theoretical pI: Translated: 6.69; Mature: 6.69
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPKEAMTADLQTAPAAGQYELSHLRSLEAEAIHIIREVAAEFERPVLLFSGGKDSIVMLH CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH LALRAFRPGRLPFPVMHVDTGHNFDEVIATRDELVKQHGLRLVVASVQDDIDAGRVVEPK HHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCEECCC QGRNPIQTVTLLRAIRENKFDAAFGGARRDEEKARAKERVFSFRDEFGQWDPKAQRPELW CCCCHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH NLYNGRHHKGEHIRVFPLSNWTEFDIWSYIGAENIALPSIYYAHRRKVFRRDGMLLAVDR HHCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEECC NMQPNPDEPVFEATVRFRTVGDVTCTGCVESQAATVDEVIAETAVSRLTERGATRADDRI CCCCCCCCCHHHHHEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH SEAGMEDRKRQGYF HHCCHHHHHHCCCC >Mature Secondary Structure PKEAMTADLQTAPAAGQYELSHLRSLEAEAIHIIREVAAEFERPVLLFSGGKDSIVMLH CCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH LALRAFRPGRLPFPVMHVDTGHNFDEVIATRDELVKQHGLRLVVASVQDDIDAGRVVEPK HHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCEECCC QGRNPIQTVTLLRAIRENKFDAAFGGARRDEEKARAKERVFSFRDEFGQWDPKAQRPELW CCCCHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH NLYNGRHHKGEHIRVFPLSNWTEFDIWSYIGAENIALPSIYYAHRRKVFRRDGMLLAVDR HHCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEECC NMQPNPDEPVFEATVRFRTVGDVTCTGCVESQAATVDEVIAETAVSRLTERGATRADDRI CCCCCCCCCHHHHHEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH SEAGMEDRKRQGYF HHCCHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 12788972 [H]