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The map label for this gene is pdhC

Identifier: 41408405

GI number: 41408405

Start: 2586882

End: 2588048

Strand: Reverse

Name: pdhC

Synonym: MAP2307c

Alternate gene names: 41408405

Gene position: 2588048-2586882 (Counterclockwise)

Preceding gene: 41408406

Following gene: 41408401

Centisome position: 53.59

GC content: 69.24

Gene sequence:

>1167_bases
GTGAGCGACGAGCGCGTCAAACCGTTTCTGGTCCCGGATCTCGGCGAGGGACTCGAAGAGGTGACCGTGACGCACTGGAA
CGTCGCGGTGGGCGACGACGTCGAGCTCAACCAGGTGTTGTGCACGGTGGAAACGGCCAAGGCCGAGGTGGAGCTGCCCA
GCCCGTACGCCGGGCGGATCGTCGAAATGAACGGCGCCGAGGGCGATGTGCTCAAGGTGGGCGCGGTGCTGGTGCGGCTG
GACACCGCCCCCGAGAGCGGCGAGCCGCCGGCGGCCGAGACGGCGCCCACACTGGTCGGTTACGGCGCCGATGCCGGGAT
CGACACCAGCCGGCGGACCGGCCGCCCGCTGGCCGCCCCACCGGTGCGCAAGCTGGCCAAGGAGCTGATGGTCGACCTCG
GCTCGCTGCGGCCCCGCTCCGGTGCCGTCATCACCCGCGAGGATGTGCTCTCGGCGGCTCACGGCACCGGCAACGGGGCC
GAGGTCCGGCCGGTTCAGGGCGTCCAGGCCCGGATGGCCGACAAAATGACCTTGTCGCACAAGGAGATTCCGGCCGCGAC
GGTTAGCGTGCAGGTCGACTGCACCGCACTGGTCCGGCTGTCCGAGCGGCTTGGTCCGGCGGAGCAGAGGATAACGCCGT
TCGTGCTGACGCTGCGGCTGCTGGTCATCGCGTTGCGCCGCAACGAGATCATGAACTCGACCTGGGTCGACACCCCGCAG
GGGCCGCAGGTGCGCATCGAGCATCGGGTGCACCTGGGCGTCGCGGTGGCCACCGAACGGGGCCTGCTGGTGCCGGTCAT
TGCCGACGCACACACCAAGACGACTCGTGAATTGGCAAGTCGGGCAGCAGAATTGATCTCCGGCGCGCGTGCCGGCTCGC
TCACGCCGGGGGAGCTGCGGGGATCGACGTTCACCGTGTCGAACTTCGGGGCGCTGGGCGTGGACGACGGTGTGCCCGTG
ATCAACCACCCCGAAGCGGCCATCCTGGGGATGGGTGCGATCAAGCCACGCCCGGTGGCCATCGGCACGGAGGTCGTGGT
GCGCCCGACCATGTCGCTCAGCGCCGTGTTCGACCACCGCATCGCCGACGGCGCGCAGGCGGCTCGATTCGTTTGCGAGC
TGCGGGATTTGATCGAATCGCCGGAAACCGCGCTGCTGGATTTGTGA

Upstream 100 bases:

>100_bases
GCGGATTCGACACCCCCTACCCGCCGGCCCGGCTGGAGAAGTGGTGGCTGCCGGGCCCGGACCGGCTGCTGGACTGCGTC
GAGCGCACGTTGGAGCTGCC

Downstream 100 bases:

>100_bases
GCCTACTTGCGTTGCGCGGCGGCCAATCGCTGTGCGAACTCCGGTGATTGAATGGAATGTGCCTGCGGGCCCAGCTCGGT
GCGCATGGCGTGCTGATGCT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSDERVKPFLVPDLGEGLEEVTVTHWNVAVGDDVELNQVLCTVETAKAEVELPSPYAGRIVEMNGAEGDVLKVGAVLVRL
DTAPESGEPPAAETAPTLVGYGADAGIDTSRRTGRPLAAPPVRKLAKELMVDLGSLRPRSGAVITREDVLSAAHGTGNGA
EVRPVQGVQARMADKMTLSHKEIPAATVSVQVDCTALVRLSERLGPAEQRITPFVLTLRLLVIALRRNEIMNSTWVDTPQ
GPQVRIEHRVHLGVAVATERGLLVPVIADAHTKTTRELASRAAELISGARAGSLTPGELRGSTFTVSNFGALGVDDGVPV
INHPEAAILGMGAIKPRPVAIGTEVVVRPTMSLSAVFDHRIADGAQAARFVCELRDLIESPETALLDL

Sequences:

>Translated_388_residues
MSDERVKPFLVPDLGEGLEEVTVTHWNVAVGDDVELNQVLCTVETAKAEVELPSPYAGRIVEMNGAEGDVLKVGAVLVRL
DTAPESGEPPAAETAPTLVGYGADAGIDTSRRTGRPLAAPPVRKLAKELMVDLGSLRPRSGAVITREDVLSAAHGTGNGA
EVRPVQGVQARMADKMTLSHKEIPAATVSVQVDCTALVRLSERLGPAEQRITPFVLTLRLLVIALRRNEIMNSTWVDTPQ
GPQVRIEHRVHLGVAVATERGLLVPVIADAHTKTTRELASRAAELISGARAGSLTPGELRGSTFTVSNFGALGVDDGVPV
INHPEAAILGMGAIKPRPVAIGTEVVVRPTMSLSAVFDHRIADGAQAARFVCELRDLIESPETALLDL
>Mature_387_residues
SDERVKPFLVPDLGEGLEEVTVTHWNVAVGDDVELNQVLCTVETAKAEVELPSPYAGRIVEMNGAEGDVLKVGAVLVRLD
TAPESGEPPAAETAPTLVGYGADAGIDTSRRTGRPLAAPPVRKLAKELMVDLGSLRPRSGAVITREDVLSAAHGTGNGAE
VRPVQGVQARMADKMTLSHKEIPAATVSVQVDCTALVRLSERLGPAEQRITPFVLTLRLLVIALRRNEIMNSTWVDTPQG
PQVRIEHRVHLGVAVATERGLLVPVIADAHTKTTRELASRAAELISGARAGSLTPGELRGSTFTVSNFGALGVDDGVPVI
NHPEAAILGMGAIKPRPVAIGTEVVVRPTMSLSAVFDHRIADGAQAARFVCELRDLIESPETALLDL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=431, Percent_Identity=29.0023201856148, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI31711992, Length=300, Percent_Identity=29, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI19923748, Length=239, Percent_Identity=32.6359832635983, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI203098816, Length=225, Percent_Identity=28.8888888888889, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI203098753, Length=235, Percent_Identity=28.5106382978723, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI260898739, Length=139, Percent_Identity=36.6906474820144, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786305, Length=416, Percent_Identity=30.5288461538462, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786946, Length=405, Percent_Identity=27.6543209876543, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=435, Percent_Identity=28.2758620689655, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25146366, Length=409, Percent_Identity=27.1393643031785, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17537937, Length=422, Percent_Identity=26.303317535545, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17538894, Length=238, Percent_Identity=26.0504201680672, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320352, Length=392, Percent_Identity=28.3163265306122, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6324258, Length=230, Percent_Identity=27.3913043478261, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI18859875, Length=430, Percent_Identity=28.6046511627907, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24645909, Length=141, Percent_Identity=39.7163120567376, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 41069; Mature: 40937

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDERVKPFLVPDLGEGLEEVTVTHWNVAVGDDVELNQVLCTVETAKAEVELPSPYAGRI
CCCCCCCCEECCCHHCCHHHEEEEEEEEEECCCCCCCCEEEEEEECCEEEECCCCCCCEE
VEMNGAEGDVLKVGAVLVRLDTAPESGEPPAAETAPTLVGYGADAGIDTSRRTGRPLAAP
EEECCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCC
PVRKLAKELMVDLGSLRPRSGAVITREDVLSAAHGTGNGAEVRPVQGVQARMADKMTLSH
HHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHCCC
KEIPAATVSVQVDCTALVRLSERLGPAEQRITPFVLTLRLLVIALRRNEIMNSTWVDTPQ
CCCCCEEEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPQVRIEHRVHLGVAVATERGLLVPVIADAHTKTTRELASRAAELISGARAGSLTPGELR
CCCEEEEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GSTFTVSNFGALGVDDGVPVINHPEAAILGMGAIKPRPVAIGTEVVVRPTMSLSAVFDHR
CCEEEECCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCEEECCEEEEECCCHHHHHHHHH
IADGAQAARFVCELRDLIESPETALLDL
HCCHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
SDERVKPFLVPDLGEGLEEVTVTHWNVAVGDDVELNQVLCTVETAKAEVELPSPYAGRI
CCCCCCCEECCCHHCCHHHEEEEEEEEEECCCCCCCCEEEEEEECCEEEECCCCCCCEE
VEMNGAEGDVLKVGAVLVRLDTAPESGEPPAAETAPTLVGYGADAGIDTSRRTGRPLAAP
EEECCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCC
PVRKLAKELMVDLGSLRPRSGAVITREDVLSAAHGTGNGAEVRPVQGVQARMADKMTLSH
HHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHCCC
KEIPAATVSVQVDCTALVRLSERLGPAEQRITPFVLTLRLLVIALRRNEIMNSTWVDTPQ
CCCCCEEEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPQVRIEHRVHLGVAVATERGLLVPVIADAHTKTTRELASRAAELISGARAGSLTPGELR
CCCEEEEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GSTFTVSNFGALGVDDGVPVINHPEAAILGMGAIKPRPVAIGTEVVVRPTMSLSAVFDHR
CCEEEECCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCEEECCEEEEECCCHHHHHHHHH
IADGAQAARFVCELRDLIESPETALLDL
HCCHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA