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The map label for this gene is nirA_2

Identifier: 41408306

GI number: 41408306

Start: 2454894

End: 2456561

Strand: Direct

Name: nirA_2

Synonym: MAP2208

Alternate gene names: 41408306

Gene position: 2454894-2456561 (Clockwise)

Preceding gene: 41408304

Following gene: 41408307

Centisome position: 50.83

GC content: 66.55

Gene sequence:

>1668_bases
ATGACCACCGCCCGACCCGCGAAGGCCCGTAACGAGGGCCAGTGGGCACTGGGGAATCGTGAGCCGCTGAACCCGAACGA
GGAAATGAAACAGGCCGGCGCCCCGCTGGCAGTTCGGGAGCGGATCGAGACCATCTACGCCAAGAACGGCTTCGACAGCA
TCGACAAGAGCGACCTGCGCGGCCGTTTCCGCTGGTGGGGCCTGTACACCCAGCGCGAGCAGGGTTACGACGGCAGCTGG
ACCGGCGACGAGAACATCGAAAAGCTCGAGGCCAGGTACTTCATGATGCGGGTGCGCTGCGACGGCGGCGCCATCTCCGC
GGCCGCGCTGCGCACGCTGGGCCAGATTTCGGTGGACTTCGCCCGCGACACCGCCGACATCACCGACCGCGAGAACATCC
AGTATCACTGGATCGAGGTGGAAAACGTCCCCGAGATCTGGCGCAGGCTCGACGCGGTGGGGTTGCGCACCACCGAGGCC
TGCGGCGACTGCCCGCGGGTGATCCTGGGGTCGCCGCTGGCGGGTGAGTCGCTCGAGGAGGTCATCGACCCGAGCTGGGC
GATCGCCGAGATCGCCCGTCGCTACATCGGCCAGCCCGACTTCGCCGACCTGCCCCGCAAGTACAAGACCGCGATCTCGG
GGTTGCAGGACGTCGCCCACGAGGTCAACGACGTCGCGTTCATCGGGGTCAACCATCCCGAGCACGGTCCCGGCCTGGAC
CTGTGGGTCGGCGGCGGGCTGTCGACCAACCCGATGCTGGCCCAACGGGTCGGCGCCTGGGTTCCGCTGCACGAGGTGCC
CGAGGTGTGGGCGGCGGTCACCTCGGTGTTCCGCGACTACGGGTACCGCCGGCTGCGCTCCAAGGCGCGGCTGAAGTTCC
TGGTCAAGGACTGGGGCATCGAGAAGTTCCGCGAAGTTCTCGAAACCGAATATCTCAAGCGTCCGCTGATCGACGGTCCG
GCCCCCGAGCCGGTCGCGCATCCGATCGATCACGTGGGGGTGCAACGGCTCAAGAACGGGCTCAACGCGGTGGGAGTCGC
CCCGATCGCCGGGCGGGTGTCGGGCACCATCCTGCTGGCGGTGGCCGACCTGGCGCAGCAAGCGGGTTGCGACCGGATCC
GATTCACTCCCTATCAGAAGCTGGTGCTGCTCGACATCCCCGACGACAAGCTCGACGAGGTGGTGGCCGGGCTGGAAGCC
CTCGGCCTGCAATCGCAACCGTCGCATTGGCGTCGTAACCTGATGGCCTGCAGCGGAATTGAGTTCTGCAAGCTGTCTTT
CGCCGAAACACGGGTGCGGGCACAGGGTTTGGTGCCCGAGCTGGAGCGCCGCCTCGCCGATGTCAACCGGCAGCTCGACG
TGCCGATTACCATCAACCTCAACGGCTGCCCCAACTCGTGTGCCCGCATCCAGGTCGCCGACATCGGCCTCAAAGGCCAG
ATGGTCGACGACGGCGAGGGCGGCTCGGTCGAGGGATTCCAGGTGCATCTGGGCGGCAGCTTGGGCCAGGACAGCGGCTT
CGGCCGAAAACTGCGCCAGCACAAGGTCACCAGCGACGAGCTCGGCGACTACATCGAGCGGGTGGCACGCAACTTCGTGA
AATATCGCGGCGAAGGTGAACGTTTCGCCCAGTGGGCCATGAGGGCAGACGAGGACGATTTGCGATGA

Upstream 100 bases:

>100_bases
GCCCCCCGCGTCTCCAGCCGGCTGACGGTTCCATGCCGCCCAACAGTGTCGGTGAACTGTGCTGGCCGCGCTGGCGATCT
TTTTCCGAGGAGAACGTCCG

Downstream 100 bases:

>100_bases
CCGAACGAACGACCAAACTTCCAGAGGCCGAACTGCGTGAACTAGCCGCCCGCGGCGCCGCCGAGCTCGAAGGCGCCAGC
GCCAGCGACGTGTTGCGTTG

Product: NirA_2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MTTARPAKARNEGQWALGNREPLNPNEEMKQAGAPLAVRERIETIYAKNGFDSIDKSDLRGRFRWWGLYTQREQGYDGSW
TGDENIEKLEARYFMMRVRCDGGAISAAALRTLGQISVDFARDTADITDRENIQYHWIEVENVPEIWRRLDAVGLRTTEA
CGDCPRVILGSPLAGESLEEVIDPSWAIAEIARRYIGQPDFADLPRKYKTAISGLQDVAHEVNDVAFIGVNHPEHGPGLD
LWVGGGLSTNPMLAQRVGAWVPLHEVPEVWAAVTSVFRDYGYRRLRSKARLKFLVKDWGIEKFREVLETEYLKRPLIDGP
APEPVAHPIDHVGVQRLKNGLNAVGVAPIAGRVSGTILLAVADLAQQAGCDRIRFTPYQKLVLLDIPDDKLDEVVAGLEA
LGLQSQPSHWRRNLMACSGIEFCKLSFAETRVRAQGLVPELERRLADVNRQLDVPITINLNGCPNSCARIQVADIGLKGQ
MVDDGEGGSVEGFQVHLGGSLGQDSGFGRKLRQHKVTSDELGDYIERVARNFVKYRGEGERFAQWAMRADEDDLR

Sequences:

>Translated_555_residues
MTTARPAKARNEGQWALGNREPLNPNEEMKQAGAPLAVRERIETIYAKNGFDSIDKSDLRGRFRWWGLYTQREQGYDGSW
TGDENIEKLEARYFMMRVRCDGGAISAAALRTLGQISVDFARDTADITDRENIQYHWIEVENVPEIWRRLDAVGLRTTEA
CGDCPRVILGSPLAGESLEEVIDPSWAIAEIARRYIGQPDFADLPRKYKTAISGLQDVAHEVNDVAFIGVNHPEHGPGLD
LWVGGGLSTNPMLAQRVGAWVPLHEVPEVWAAVTSVFRDYGYRRLRSKARLKFLVKDWGIEKFREVLETEYLKRPLIDGP
APEPVAHPIDHVGVQRLKNGLNAVGVAPIAGRVSGTILLAVADLAQQAGCDRIRFTPYQKLVLLDIPDDKLDEVVAGLEA
LGLQSQPSHWRRNLMACSGIEFCKLSFAETRVRAQGLVPELERRLADVNRQLDVPITINLNGCPNSCARIQVADIGLKGQ
MVDDGEGGSVEGFQVHLGGSLGQDSGFGRKLRQHKVTSDELGDYIERVARNFVKYRGEGERFAQWAMRADEDDLR
>Mature_554_residues
TTARPAKARNEGQWALGNREPLNPNEEMKQAGAPLAVRERIETIYAKNGFDSIDKSDLRGRFRWWGLYTQREQGYDGSWT
GDENIEKLEARYFMMRVRCDGGAISAAALRTLGQISVDFARDTADITDRENIQYHWIEVENVPEIWRRLDAVGLRTTEAC
GDCPRVILGSPLAGESLEEVIDPSWAIAEIARRYIGQPDFADLPRKYKTAISGLQDVAHEVNDVAFIGVNHPEHGPGLDL
WVGGGLSTNPMLAQRVGAWVPLHEVPEVWAAVTSVFRDYGYRRLRSKARLKFLVKDWGIEKFREVLETEYLKRPLIDGPA
PEPVAHPIDHVGVQRLKNGLNAVGVAPIAGRVSGTILLAVADLAQQAGCDRIRFTPYQKLVLLDIPDDKLDEVVAGLEAL
GLQSQPSHWRRNLMACSGIEFCKLSFAETRVRAQGLVPELERRLADVNRQLDVPITINLNGCPNSCARIQVADIGLKGQM
VDDGEGGSVEGFQVHLGGSLGQDSGFGRKLRQHKVTSDELGDYIERVARNFVKYRGEGERFAQWAMRADEDDLR

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=536, Percent_Identity=24.2537313432836, Blast_Score=113, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6322597, Length=563, Percent_Identity=25.7548845470693, Blast_Score=129, Evalue=1e-30,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SIR2_MYCPA (Q73XV0)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_961142.1
- ProteinModelPortal:   Q73XV0
- SMR:   Q73XV0
- EnsemblBacteria:   EBMYCT00000037265
- GeneID:   2718803
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP2208
- NMPDR:   fig|262316.1.peg.2208
- GeneTree:   EBGT00050000015728
- HOGENOM:   HBG578916
- OMA:   DQLERWQ
- ProtClustDB:   CLSK872057
- BRENDA:   1.8.7.1
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.7.1

Molecular weight: Translated: 61869; Mature: 61738

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTARPAKARNEGQWALGNREPLNPNEEMKQAGAPLAVRERIETIYAKNGFDSIDKSDLR
CCCCCCCCCCCCCCEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHC
GRFRWWGLYTQREQGYDGSWTGDENIEKLEARYFMMRVRCDGGAISAAALRTLGQISVDF
CCEEEEEEEEHHCCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHH
ARDTADITDRENIQYHWIEVENVPEIWRRLDAVGLRTTEACGDCPRVILGSPLAGESLEE
HCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCHHHHH
VIDPSWAIAEIARRYIGQPDFADLPRKYKTAISGLQDVAHEVNDVAFIGVNHPEHGPGLD
HHCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCE
LWVGGGLSTNPMLAQRVGAWVPLHEVPEVWAAVTSVFRDYGYRRLRSKARLKFLVKDWGI
EEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EKFREVLETEYLKRPLIDGPAPEPVAHPIDHVGVQRLKNGLNAVGVAPIAGRVSGTILLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
VADLAQQAGCDRIRFTPYQKLVLLDIPDDKLDEVVAGLEALGLQSQPSHWRRNLMACSGI
HHHHHHHCCCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
EFCKLSFAETRVRAQGLVPELERRLADVNRQLDVPITINLNGCPNSCARIQVADIGLKGQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCE
MVDDGEGGSVEGFQVHLGGSLGQDSGFGRKLRQHKVTSDELGDYIERVARNFVKYRGEGE
EEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCH
RFAQWAMRADEDDLR
HHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTARPAKARNEGQWALGNREPLNPNEEMKQAGAPLAVRERIETIYAKNGFDSIDKSDLR
CCCCCCCCCCCCCEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHC
GRFRWWGLYTQREQGYDGSWTGDENIEKLEARYFMMRVRCDGGAISAAALRTLGQISVDF
CCEEEEEEEEHHCCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHH
ARDTADITDRENIQYHWIEVENVPEIWRRLDAVGLRTTEACGDCPRVILGSPLAGESLEE
HCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCHHHHH
VIDPSWAIAEIARRYIGQPDFADLPRKYKTAISGLQDVAHEVNDVAFIGVNHPEHGPGLD
HHCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCE
LWVGGGLSTNPMLAQRVGAWVPLHEVPEVWAAVTSVFRDYGYRRLRSKARLKFLVKDWGI
EEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EKFREVLETEYLKRPLIDGPAPEPVAHPIDHVGVQRLKNGLNAVGVAPIAGRVSGTILLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
VADLAQQAGCDRIRFTPYQKLVLLDIPDDKLDEVVAGLEALGLQSQPSHWRRNLMACSGI
HHHHHHHCCCCCEEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
EFCKLSFAETRVRAQGLVPELERRLADVNRQLDVPITINLNGCPNSCARIQVADIGLKGQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCE
MVDDGEGGSVEGFQVHLGGSLGQDSGFGRKLRQHKVTSDELGDYIERVARNFVKYRGEGE
EEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCH
RFAQWAMRADEDDLR
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA