Click here to switch to the map view.

The map label for this gene is ephD [H]

Identifier: 41408296

GI number: 41408296

Start: 2443089

End: 2443937

Strand: Direct

Name: ephD [H]

Synonym: MAP2198

Alternate gene names: 41408296

Gene position: 2443089-2443937 (Clockwise)

Preceding gene: 41408295

Following gene: 41408297

Centisome position: 50.58

GC content: 68.67

Gene sequence:

>849_bases
ATGTTGGACCGGCTGCTGCACCGATCGAAGACCAGCCGCGGCGCCCTGGCGGTGGTGACCGGCGCGGGAAGCGGCATCGG
TGCGGCCTTCGCCCTCGAACTGGGCAAGCGCGGCGGCACCGTCGTGTGCAGCGATATCGACCAAGCCGCCGCCCAGCGGA
CGGCCGACGCGATCACCCAACACGGCGCGAAAGCCCTCGCAACCCGTTGTGATGTCTCGCAATTCGGCGACGTGCAGGCG
CTGGCCGAGCAGTCGCAGTCCTGCTTCGGCGCCCCACCCACACTGGTGATCAACAACGCCGGTGTCGGCGCCGGCGGCGC
GGCCATCGGTGATGCGCCACTGGACGATTGGCAGTGGACGCTCGGAATCAACCTGTGGGGTCCCATTCACGGCTGTCATG
TGTTCACCCCGATCCTGCGCGACGCCGCGCCGTCGGCCGCGCCGCGGGGCATCATCAACGTCGCCTCGGCCGCGGCGTTC
GGCGCGGCCCCCGGCATGGCTGCCTACAACGTCAGCAAGGCGGGCGTGCTGTCACTGTCCGAGACCCTGGCCGCCGAGCT
GTCCGGCACGCCGGTGCGGGTCACCGTGCTGTGCCCGACGTTCGTCAAGACGAACATCCTCGAATCCGGGCGAATCAGCG
AGGAGTCCGGCGAACTGGCCGCAAAACTGATGCGCTGGACCGGGTTCTCCGCGGACAAAGTCGCCCGCATCTGCTTGGAC
GCGCACGACCGCGGCGACCTGTACTGCATGCCGCAGCTCGACGCACAAATCGGCTGGCACATCAAACGCCTTGCCCCGCA
GGCCTATACGCGAGCAGCCGGCCTGGTGTCCCGAATCAACCTGCCCTGA

Upstream 100 bases:

>100_bases
CGCATGACGCCCGAAGCCGCCAAGGCCATGGCCTATGTCGCGCGGTTCATCACCCACGCCCTGCAGGATGCCCGTGCCCT
CACCCAGGAGATGCACTGAC

Downstream 100 bases:

>100_bases
TCACCCGACGGAAAGGAGCCTCCGGTGGCCATCGACATGGAAGCCATGCTCGCCAAGATCAAGGATCGACAGTGGGCGCT
CGCCGACATCGATTGGACCG

Product: hypothetical protein

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MLDRLLHRSKTSRGALAVVTGAGSGIGAAFALELGKRGGTVVCSDIDQAAAQRTADAITQHGAKALATRCDVSQFGDVQA
LAEQSQSCFGAPPTLVINNAGVGAGGAAIGDAPLDDWQWTLGINLWGPIHGCHVFTPILRDAAPSAAPRGIINVASAAAF
GAAPGMAAYNVSKAGVLSLSETLAAELSGTPVRVTVLCPTFVKTNILESGRISEESGELAAKLMRWTGFSADKVARICLD
AHDRGDLYCMPQLDAQIGWHIKRLAPQAYTRAAGLVSRINLP

Sequences:

>Translated_282_residues
MLDRLLHRSKTSRGALAVVTGAGSGIGAAFALELGKRGGTVVCSDIDQAAAQRTADAITQHGAKALATRCDVSQFGDVQA
LAEQSQSCFGAPPTLVINNAGVGAGGAAIGDAPLDDWQWTLGINLWGPIHGCHVFTPILRDAAPSAAPRGIINVASAAAF
GAAPGMAAYNVSKAGVLSLSETLAAELSGTPVRVTVLCPTFVKTNILESGRISEESGELAAKLMRWTGFSADKVARICLD
AHDRGDLYCMPQLDAQIGWHIKRLAPQAYTRAAGLVSRINLP
>Mature_282_residues
MLDRLLHRSKTSRGALAVVTGAGSGIGAAFALELGKRGGTVVCSDIDQAAAQRTADAITQHGAKALATRCDVSQFGDVQA
LAEQSQSCFGAPPTLVINNAGVGAGGAAIGDAPLDDWQWTLGINLWGPIHGCHVFTPILRDAAPSAAPRGIINVASAAAF
GAAPGMAAYNVSKAGVLSLSETLAAELSGTPVRVTVLCPTFVKTNILESGRISEESGELAAKLMRWTGFSADKVARICLD
AHDRGDLYCMPQLDAQIGWHIKRLAPQAYTRAAGLVSRINLP

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=204, Percent_Identity=35.7843137254902, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI32483357, Length=213, Percent_Identity=30.5164319248826, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI40807363, Length=204, Percent_Identity=27.9411764705882, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI25282469, Length=236, Percent_Identity=26.6949152542373, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI31542939, Length=211, Percent_Identity=31.7535545023697, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI142976729, Length=227, Percent_Identity=29.5154185022026, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI126723191, Length=185, Percent_Identity=30.8108108108108, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI310128830, Length=268, Percent_Identity=26.1194029850746, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI310115468, Length=268, Percent_Identity=26.1194029850746, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI33667109, Length=213, Percent_Identity=30.0469483568075, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI5031737, Length=194, Percent_Identity=30.9278350515464, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI126723750, Length=220, Percent_Identity=28.1818181818182, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4758504, Length=207, Percent_Identity=28.5024154589372, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787905, Length=196, Percent_Identity=32.6530612244898, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082100, Length=196, Percent_Identity=29.0816326530612, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI2367175, Length=199, Percent_Identity=30.6532663316583, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789208, Length=254, Percent_Identity=26.7716535433071, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787335, Length=211, Percent_Identity=27.4881516587678, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17564282, Length=201, Percent_Identity=28.3582089552239, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25147288, Length=196, Percent_Identity=32.6530612244898, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193203115, Length=201, Percent_Identity=28.3582089552239, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17555706, Length=206, Percent_Identity=28.6407766990291, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI193203117, Length=197, Percent_Identity=27.9187817258883, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17567345, Length=216, Percent_Identity=28.2407407407407, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24649181, Length=200, Percent_Identity=31, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28571526, Length=205, Percent_Identity=32.1951219512195, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21357041, Length=207, Percent_Identity=31.8840579710145, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24639444, Length=191, Percent_Identity=30.8900523560209, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364892, Length=212, Percent_Identity=27.8301886792453, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI23397609, Length=201, Percent_Identity=32.3383084577114, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17737361, Length=202, Percent_Identity=29.2079207920792, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644339, Length=204, Percent_Identity=28.4313725490196, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21355319, Length=212, Percent_Identity=29.7169811320755, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI22024069, Length=206, Percent_Identity=30.0970873786408, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 29181; Mature: 29181

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRLLHRSKTSRGALAVVTGAGSGIGAAFALELGKRGGTVVCSDIDQAAAQRTADAITQ
CHHHHHHHCCCCCCCEEEEECCCCCCCHHHHEECCCCCCEEEECCHHHHHHHHHHHHHHH
HGAKALATRCDVSQFGDVQALAEQSQSCFGAPPTLVINNAGVGAGGAAIGDAPLDDWQWT
HHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEE
LGINLWGPIHGCHVFTPILRDAAPSAAPRGIINVASAAAFGAAPGMAAYNVSKAGVLSLS
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHH
ETLAAELSGTPVRVTVLCPTFVKTNILESGRISEESGELAAKLMRWTGFSADKVARICLD
HHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHC
AHDRGDLYCMPQLDAQIGWHIKRLAPQAYTRAAGLVSRINLP
CCCCCCEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDRLLHRSKTSRGALAVVTGAGSGIGAAFALELGKRGGTVVCSDIDQAAAQRTADAITQ
CHHHHHHHCCCCCCCEEEEECCCCCCCHHHHEECCCCCCEEEECCHHHHHHHHHHHHHHH
HGAKALATRCDVSQFGDVQALAEQSQSCFGAPPTLVINNAGVGAGGAAIGDAPLDDWQWT
HHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEE
LGINLWGPIHGCHVFTPILRDAAPSAAPRGIINVASAAAFGAAPGMAAYNVSKAGVLSLS
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHH
ETLAAELSGTPVRVTVLCPTFVKTNILESGRISEESGELAAKLMRWTGFSADKVARICLD
HHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHC
AHDRGDLYCMPQLDAQIGWHIKRLAPQAYTRAAGLVSRINLP
CCCCCCEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]