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The map label for this gene is dhbF [H]

Identifier: 41408270

GI number: 41408270

Start: 2406269

End: 2407534

Strand: Reverse

Name: dhbF [H]

Synonym: MAP2172c

Alternate gene names: 41408270

Gene position: 2407534-2406269 (Counterclockwise)

Preceding gene: 41408271

Following gene: 41408269

Centisome position: 49.85

GC content: 71.41

Gene sequence:

>1266_bases
ATGATCCTGGCCGACGACGCCGAACACCGCGACCCCGAGGCGCTCGGGGAGTTGATGCACCGGCATTCGGTCGCCCAGGT
GACCGCGGTGCCCAGCCTGGTCTCCGCGCTGCTGGACAGCCGGCCCGATGCGGTGCGCTCGCTGTCGCGGCTGGTGTGCG
GCGGCGAGCCGGTCAGCACCTCGCTGCTGCAGCGGCTGGTGTCGGTGTGCGACGAAGCCGACGGCGGCGGCCCCGAGCTG
TTGAACAACATCGGCTCGACCGAAACCTCCGGCGCGGTGTCCCGCGGGCCGTTGAGTCCGCCGAATCCGCTTGTCGGGAA
ACCCGTTCCGGGTGCGCAGGCGTATCTGCTCGACGACGGGCTGCGCCCGGTGCCGGTCGGCGTGGTGGGGGAGCTCTACT
ACGCCGGCGACCAGCTCGCTCGGGGGTATTGGAAACGTCCCGGCCTGACCGCGGCCCGGTTCGTCGCGAATCCGTTCGGG
GCGGAACCCGGTTCGCGCCTGTACCGCAGCGGCGACCTGGCCCGGTGGACCGAGGACGGCCAGCTGGAATTCGTCGGGCG
CTCCGACCACCAGGTGCAGGTCCGTGGTTTCCGCGTCGAGTTGGCCGAGGTCGAGGCGGCTCTGGCGGGCGCCGATGGCG
TCGCCGCGGCGGCGGCCCGCACCTGGGAGGTGCACGGCGGCACCTCCCTGGCCGGCTACGTGGTGCCGCAACGGCCCATT
GCCGACGAGGCCGAGAAGGCGGCGTTCGCCGCACAGGTGCGCGCCGAGATCGCTGCCACCTTGCCGGGTTACATGATGCC
GTCGTCGCTGACCGTGCTCGACGCCCTGCCGAAAACCGAATCCGGCAAGCTGAATCGGCCCGGCCTGCCCCGGCCGGTGG
TCAGCACCGGCGGCCGGACCGAGCCGACCCGCACCGACACCGAACGGGCATTGGCCAACGTGTTCGCCGAGCTGCTGTCC
ACACCCGAGGTCGGGCGGTTCGACGACTTCTTCGCGCTGGGCGGCGACAGCATCCTGTCGGTGCAGCTGGCGTCGCGGGC
GCGGGCAGCCGGCCTGCCGGTGAGTCCGCGGATGATCTTCGAGAACCCCACCGTGCAGCAGCTGGCTGCCGCCCTGGATG
CGTTGGGAGACAACGATAGTGACGGTCGGCTGGACGATCAGCCGGCCGACGCCCGGTTCGAGCCGATGAGCACCTCAGGG
TTGTCGGCCTCGGATCTGGCCGCGGTGACCCAGCTTTGGTCGTCGTCGCGGGAAGGCACCGCATGA

Upstream 100 bases:

>100_bases
CTACCCGCCGCGCACCGACGACGTTCGATTGGCGCAGGCCTCCATCACCTTCCTGGAAGGCGGCATGGAGATGCTGGCCG
GGCTGGCGGCGGGCGCGACG

Downstream 100 bases:

>100_bases
CCGCCGCCGAAACCGATGTCGCCCCCGACATCGAGGACGTGATGGCGCTGAGCCCGCTGCAGGAGGGCTTGTATTCGCTG
ACCACGCTTGCCGAGTTCGC

Product: hypothetical protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MILADDAEHRDPEALGELMHRHSVAQVTAVPSLVSALLDSRPDAVRSLSRLVCGGEPVSTSLLQRLVSVCDEADGGGPEL
LNNIGSTETSGAVSRGPLSPPNPLVGKPVPGAQAYLLDDGLRPVPVGVVGELYYAGDQLARGYWKRPGLTAARFVANPFG
AEPGSRLYRSGDLARWTEDGQLEFVGRSDHQVQVRGFRVELAEVEAALAGADGVAAAAARTWEVHGGTSLAGYVVPQRPI
ADEAEKAAFAAQVRAEIAATLPGYMMPSSLTVLDALPKTESGKLNRPGLPRPVVSTGGRTEPTRTDTERALANVFAELLS
TPEVGRFDDFFALGGDSILSVQLASRARAAGLPVSPRMIFENPTVQQLAAALDALGDNDSDGRLDDQPADARFEPMSTSG
LSASDLAAVTQLWSSSREGTA

Sequences:

>Translated_421_residues
MILADDAEHRDPEALGELMHRHSVAQVTAVPSLVSALLDSRPDAVRSLSRLVCGGEPVSTSLLQRLVSVCDEADGGGPEL
LNNIGSTETSGAVSRGPLSPPNPLVGKPVPGAQAYLLDDGLRPVPVGVVGELYYAGDQLARGYWKRPGLTAARFVANPFG
AEPGSRLYRSGDLARWTEDGQLEFVGRSDHQVQVRGFRVELAEVEAALAGADGVAAAAARTWEVHGGTSLAGYVVPQRPI
ADEAEKAAFAAQVRAEIAATLPGYMMPSSLTVLDALPKTESGKLNRPGLPRPVVSTGGRTEPTRTDTERALANVFAELLS
TPEVGRFDDFFALGGDSILSVQLASRARAAGLPVSPRMIFENPTVQQLAAALDALGDNDSDGRLDDQPADARFEPMSTSG
LSASDLAAVTQLWSSSREGTA
>Mature_421_residues
MILADDAEHRDPEALGELMHRHSVAQVTAVPSLVSALLDSRPDAVRSLSRLVCGGEPVSTSLLQRLVSVCDEADGGGPEL
LNNIGSTETSGAVSRGPLSPPNPLVGKPVPGAQAYLLDDGLRPVPVGVVGELYYAGDQLARGYWKRPGLTAARFVANPFG
AEPGSRLYRSGDLARWTEDGQLEFVGRSDHQVQVRGFRVELAEVEAALAGADGVAAAAARTWEVHGGTSLAGYVVPQRPI
ADEAEKAAFAAQVRAEIAATLPGYMMPSSLTVLDALPKTESGKLNRPGLPRPVVSTGGRTEPTRTDTERALANVFAELLS
TPEVGRFDDFFALGGDSILSVQLASRARAAGLPVSPRMIFENPTVQQLAAALDALGDNDSDGRLDDQPADARFEPMSTSG
LSASDLAAVTQLWSSSREGTA

Specific function: Specifically adenylates threonine and glycine, and loads them onto their corresponding peptidyl carrier domains [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Escherichia coli, GI1786801, Length=409, Percent_Identity=33.9853300733496, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1788107, Length=283, Percent_Identity=29.6819787985866, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI145693145, Length=288, Percent_Identity=28.125, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786810, Length=285, Percent_Identity=28.4210526315789, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17556356, Length=340, Percent_Identity=27.9411764705882, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319591, Length=366, Percent_Identity=27.0491803278689, Blast_Score=106, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648676, Length=415, Percent_Identity=27.2289156626506, Blast_Score=125, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 44180; Mature: 44180

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILADDAEHRDPEALGELMHRHSVAQVTAVPSLVSALLDSRPDAVRSLSRLVCGGEPVST
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
SLLQRLVSVCDEADGGGPELLNNIGSTETSGAVSRGPLSPPNPLVGKPVPGAQAYLLDDG
HHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
LRPVPVGVVGELYYAGDQLARGYWKRPGLTAARFVANPFGAEPGSRLYRSGDLARWTEDG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHCCCCCEECCCC
QLEFVGRSDHQVQVRGFRVELAEVEAALAGADGVAAAAARTWEVHGGTSLAGYVVPQRPI
CEEEECCCCCEEEEEEEEEEHHHHHHHHHCCCCHHHHHHHEEEECCCCCEEEEECCCCCC
ADEAEKAAFAAQVRAEIAATLPGYMMPSSLTVLDALPKTESGKLNRPGLPRPVVSTGGRT
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCC
EPTRTDTERALANVFAELLSTPEVGRFDDFFALGGDSILSVQLASRARAAGLPVSPRMIF
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCCCCEEEE
ENPTVQQLAAALDALGDNDSDGRLDDQPADARFEPMSTSGLSASDLAAVTQLWSSSREGT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
A
C
>Mature Secondary Structure
MILADDAEHRDPEALGELMHRHSVAQVTAVPSLVSALLDSRPDAVRSLSRLVCGGEPVST
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
SLLQRLVSVCDEADGGGPELLNNIGSTETSGAVSRGPLSPPNPLVGKPVPGAQAYLLDDG
HHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
LRPVPVGVVGELYYAGDQLARGYWKRPGLTAARFVANPFGAEPGSRLYRSGDLARWTEDG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHCCCCCEECCCC
QLEFVGRSDHQVQVRGFRVELAEVEAALAGADGVAAAAARTWEVHGGTSLAGYVVPQRPI
CEEEECCCCCEEEEEEEEEEHHHHHHHHHCCCCHHHHHHHEEEECCCCCEEEEECCCCCC
ADEAEKAAFAAQVRAEIAATLPGYMMPSSLTVLDALPKTESGKLNRPGLPRPVVSTGGRT
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCC
EPTRTDTERALANVFAELLSTPEVGRFDDFFALGGDSILSVQLASRARAAGLPVSPRMIF
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCCCCEEEE
ENPTVQQLAAALDALGDNDSDGRLDDQPADARFEPMSTSGLSASDLAAVTQLWSSSREGT
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11112781; 9384377; 10568751; 8550523 [H]