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The map label for this gene is amiA2

Identifier: 41408241

GI number: 41408241

Start: 2372287

End: 2373798

Strand: Direct

Name: amiA2

Synonym: MAP2143

Alternate gene names: 41408241

Gene position: 2372287-2373798 (Clockwise)

Preceding gene: 41408237

Following gene: 41408242

Centisome position: 49.12

GC content: 72.55

Gene sequence:

>1512_bases
GTGATTGGCGCTTCTGAGTCGGGTCCCGGGGCGCTTTCCGGCTCGGGTTCCGGATCCGGCAACCAGCGCCTGCCCACCCT
GACTGACCTGCTGTATCAGCTGGCCAGCCGGTCGGTGAGTTCCACCACACTGGTGGGTCGCTCGCTGCACGCTATCCATG
CCAGCCAGCCCACGCTGAACGCCTTCCGGGTGGTGCTCACCGAGTCGGCGCTGGCCGACGCCGCCGAGGCCGATCGGCGC
CGCGCGGCCGGGGACACCGCCCCGCTGCTGGGCATTCCGATCGCCGTCAAGGACGACGTCGACATTGCCGGCGTGCCCAC
CGCCTTCGGCACCGAGGGCTCGGTGCCGCCCGCCACGCACGATGCCGAGGTGGTTCGCCGCCTCAAGGCCGCCGGCGCGG
TGATCGTCGGCAAAACCAACACCTGTGAACTCGGGCAATGGCCGTTCACCAGCGGACCGGGCTTCGGGCACACCCGCAAT
CCCTGGTCGCGCCGGCACACCCCGGGCGGATCGTCGGGCGGCAGCGCCGCGGCGGTGGCCGCGGGCCTGGTGACCGCCGC
CATCGGGTCCGACGGCGCCGGCAGCATCCGCATCCCGGCGGCGTGGACGCACCTGGTGGGCATCAAGCCGCAGCGCGGAC
GCATCTCGACCTGGCCGCTGCCGGAGGCGTTCAACGGGATCACGGTCAACGGCGTGCTGGCCCGCACGGTGGCCGACGCG
GCGTTGGTGCTCGACGCGGCGTCCGGCAACGTCGACGGCGACCGGCACAAACCGCCCCCGATCACCGCCTCCGACTATGT
GGGCAAGGCGCCCGGTCCGTTGAACATCGCGCTGTCGACCCGGTTCCCCTACACGGGGTTCCGGCCGAAGCTGCATCCGG
AGATCCTGGCCGCGACCCGTGCGGTGGGCAAGCAACTCGAGCTGCTGGGCCACACCGTGGTGCCGGGCAACCCGGACTAC
GGCATGCGGCTGTCGTGGGATTTTCTCGCCCGCTCCACCGCCGGCCTGCGGGACTGGGAGGAGCGGTTGGGCGACGGCGT
GGTTCTGGACCCCCGCACGGTGGCCAACTTGCGGCTGGGTCATCTGCTGGGACAGGCGATCCTGCGCAGCGCCCGCCGCC
ACGAGGCCGCCGACCAGCGCCGGGTGGGCTCGATCTTCGACATCGTCGACGTGGTGCTGGCGCCGACCACCGCGCAACCC
CCGCCGCTGGCCCGCGCCTTCGACCGGCTCAGCGGCTTCGGCACCGACCGCGCCATGATCGCGGCGTGCCCGCTGACCTT
CCCGTGGAACGTGCTGGGCTGGCCGTCGATCAACGTTCCGGCCGGCTTCACCTCCGACGGCCTGCCGATCGGCGTGCAGC
TGATGGGCCCGGCCAACAGCGAGGGCATGCTGATCTCGCTGGCCGCCGAGTTGGAGGCGGTGTCCGGGTGGGCGAGCAAA
CAGCCGCAGCCCTGGTGGGACCAGAGCGCCGACACTCCCCCCGCCCCCGGCCAGCCCGCGCCCACCGGGTGA

Upstream 100 bases:

>100_bases
CTGGCGCAGCACAACAGCTCGGTCTCGATACAGCCGCATCCCAATAGTTTTGCACCCCGCCGCGACATTGCGGGGATCCG
CGCCGTTAGTCTCGTACCCC

Downstream 100 bases:

>100_bases
CGGCCGTCCGCGACGAGCGCGCCGGCGGCCGGCCGCGGCGAGTCGGCCGTGCACAACCGGGGTATCCGAGCGCAATGGAC
CAATCCGACGCACCTTTGCT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MIGASESGPGALSGSGSGSGNQRLPTLTDLLYQLASRSVSSTTLVGRSLHAIHASQPTLNAFRVVLTESALADAAEADRR
RAAGDTAPLLGIPIAVKDDVDIAGVPTAFGTEGSVPPATHDAEVVRRLKAAGAVIVGKTNTCELGQWPFTSGPGFGHTRN
PWSRRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFNGITVNGVLARTVADA
ALVLDAASGNVDGDRHKPPPITASDYVGKAPGPLNIALSTRFPYTGFRPKLHPEILAATRAVGKQLELLGHTVVPGNPDY
GMRLSWDFLARSTAGLRDWEERLGDGVVLDPRTVANLRLGHLLGQAILRSARRHEAADQRRVGSIFDIVDVVLAPTTAQP
PPLARAFDRLSGFGTDRAMIAACPLTFPWNVLGWPSINVPAGFTSDGLPIGVQLMGPANSEGMLISLAAELEAVSGWASK
QPQPWWDQSADTPPAPGQPAPTG

Sequences:

>Translated_503_residues
MIGASESGPGALSGSGSGSGNQRLPTLTDLLYQLASRSVSSTTLVGRSLHAIHASQPTLNAFRVVLTESALADAAEADRR
RAAGDTAPLLGIPIAVKDDVDIAGVPTAFGTEGSVPPATHDAEVVRRLKAAGAVIVGKTNTCELGQWPFTSGPGFGHTRN
PWSRRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFNGITVNGVLARTVADA
ALVLDAASGNVDGDRHKPPPITASDYVGKAPGPLNIALSTRFPYTGFRPKLHPEILAATRAVGKQLELLGHTVVPGNPDY
GMRLSWDFLARSTAGLRDWEERLGDGVVLDPRTVANLRLGHLLGQAILRSARRHEAADQRRVGSIFDIVDVVLAPTTAQP
PPLARAFDRLSGFGTDRAMIAACPLTFPWNVLGWPSINVPAGFTSDGLPIGVQLMGPANSEGMLISLAAELEAVSGWASK
QPQPWWDQSADTPPAPGQPAPTG
>Mature_503_residues
MIGASESGPGALSGSGSGSGNQRLPTLTDLLYQLASRSVSSTTLVGRSLHAIHASQPTLNAFRVVLTESALADAAEADRR
RAAGDTAPLLGIPIAVKDDVDIAGVPTAFGTEGSVPPATHDAEVVRRLKAAGAVIVGKTNTCELGQWPFTSGPGFGHTRN
PWSRRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFNGITVNGVLARTVADA
ALVLDAASGNVDGDRHKPPPITASDYVGKAPGPLNIALSTRFPYTGFRPKLHPEILAATRAVGKQLELLGHTVVPGNPDY
GMRLSWDFLARSTAGLRDWEERLGDGVVLDPRTVANLRLGHLLGQAILRSARRHEAADQRRVGSIFDIVDVVLAPTTAQP
PPLARAFDRLSGFGTDRAMIAACPLTFPWNVLGWPSINVPAGFTSDGLPIGVQLMGPANSEGMLISLAAELEAVSGWASK
QPQPWWDQSADTPPAPGQPAPTG

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=240, Percent_Identity=36.25, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI195972892, Length=229, Percent_Identity=31.8777292576419, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI166795287, Length=240, Percent_Identity=32.0833333333333, Blast_Score=65, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17537465, Length=202, Percent_Identity=32.6732673267327, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=240, Percent_Identity=32.0833333333333, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=455, Percent_Identity=26.3736263736264, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=407, Percent_Identity=27.5184275184275, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=455, Percent_Identity=24.8351648351648, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=160, Percent_Identity=36.25, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=160, Percent_Identity=36.25, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=449, Percent_Identity=27.1714922048998, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320448, Length=239, Percent_Identity=30.1255230125523, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=445, Percent_Identity=24.2696629213483, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24648113, Length=472, Percent_Identity=32.6271186440678, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=25.7425742574257, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI161078093, Length=513, Percent_Identity=27.2904483430799, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648435, Length=236, Percent_Identity=31.7796610169492, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648437, Length=236, Percent_Identity=31.7796610169492, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45550774, Length=236, Percent_Identity=31.7796610169492, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356731, Length=197, Percent_Identity=32.994923857868, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648441, Length=171, Percent_Identity=34.5029239766082, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648439, Length=171, Percent_Identity=34.5029239766082, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652985, Length=203, Percent_Identity=30.5418719211823, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922090, Length=203, Percent_Identity=30.5418719211823, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652981, Length=203, Percent_Identity=30.5418719211823, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652983, Length=203, Percent_Identity=30.5418719211823, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 52198; Mature: 52198

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGASESGPGALSGSGSGSGNQRLPTLTDLLYQLASRSVSSTTLVGRSLHAIHASQPTLN
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCHHHEEECCCCCHH
AFRVVLTESALADAAEADRRRAAGDTAPLLGIPIAVKDDVDIAGVPTAFGTEGSVPPATH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCC
DAEVVRRLKAAGAVIVGKTNTCELGQWPFTSGPGFGHTRNPWSRRHTPGGSSGGSAAAVA
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHH
AGLVTAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFNGITVNGVLARTVADA
HHHHHHHCCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCHHCCCEEECHHHHHHHHHH
ALVLDAASGNVDGDRHKPPPITASDYVGKAPGPLNIALSTRFPYTGFRPKLHPEILAATR
EEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
AVGKQLELLGHTVVPGNPDYGMRLSWDFLARSTAGLRDWEERLGDGVVLDPRTVANLRLG
HHHHHHHHHCCEECCCCCCCCCEEEHHHHHHCCCCHHHHHHHHCCCEEECCHHHHHHHHH
HLLGQAILRSARRHEAADQRRVGSIFDIVDVVLAPTTAQPPPLARAFDRLSGFGTDRAMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEE
AACPLTFPWNVLGWPSINVPAGFTSDGLPIGVQLMGPANSEGMLISLAAELEAVSGWASK
EECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEHHHHHHHHHCCC
QPQPWWDQSADTPPAPGQPAPTG
CCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIGASESGPGALSGSGSGSGNQRLPTLTDLLYQLASRSVSSTTLVGRSLHAIHASQPTLN
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCHHHEEECCCCCHH
AFRVVLTESALADAAEADRRRAAGDTAPLLGIPIAVKDDVDIAGVPTAFGTEGSVPPATH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCC
DAEVVRRLKAAGAVIVGKTNTCELGQWPFTSGPGFGHTRNPWSRRHTPGGSSGGSAAAVA
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHH
AGLVTAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFNGITVNGVLARTVADA
HHHHHHHCCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCHHCCCEEECHHHHHHHHHH
ALVLDAASGNVDGDRHKPPPITASDYVGKAPGPLNIALSTRFPYTGFRPKLHPEILAATR
EEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
AVGKQLELLGHTVVPGNPDYGMRLSWDFLARSTAGLRDWEERLGDGVVLDPRTVANLRLG
HHHHHHHHHCCEECCCCCCCCCEEEHHHHHHCCCCHHHHHHHHCCCEEECCHHHHHHHHH
HLLGQAILRSARRHEAADQRRVGSIFDIVDVVLAPTTAQPPPLARAFDRLSGFGTDRAMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEE
AACPLTFPWNVLGWPSINVPAGFTSDGLPIGVQLMGPANSEGMLISLAAELEAVSGWASK
EECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEHHHHHHHHHCCC
QPQPWWDQSADTPPAPGQPAPTG
CCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]