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The map label for this gene is dnaG

Identifier: 41408228

GI number: 41408228

Start: 2359284

End: 2361236

Strand: Reverse

Name: dnaG

Synonym: MAP2130c

Alternate gene names: 41408228

Gene position: 2361236-2359284 (Counterclockwise)

Preceding gene: 41408229

Following gene: 41408226

Centisome position: 48.89

GC content: 70.71

Gene sequence:

>1953_bases
ATGTCCAGCCCGGCAGGTTCACGCGCCCAGGGCGATGGCCGTGCCCGGGGCCGGGGCCGGATTCCCGACCGCGACATCGC
CGCCATCCGCGAACGGGTGCGCATCGATGAAGTGGTCGGCGATTACGTCCAATTGCGGCGTGCCGGTGCGGATTCGCTCA
AGGGCCTGTGCCCGTTTCACGACGAGAAGTCGCCGTCGTTTCACGTCCGCCCCAACCACGGGCACTTCCACTGCTTCGGC
TGCGGCGAGGGCGGCGACGTGTACGCGTTCCTCCAGAAGATCGAGCACGTCAGCTTCGTCGAGGCCGTCGAACTGCTCGC
CGACCGGATCGGCCACACGATCACCTACTCCGGCCCGGCCACCAGCGTGCAGCGCGACCGCGGCAGCCGCAGCCGGCTCA
TCGCGGCCAACGCGGCCGCGGCGGAGTTCTACGCGGCGGCGCTCGAGTCCGACGAGGCGGCGCCGGCCCGCCAGTATCTG
ACCGAACGCAACTTCGATGCCGAGGCGGCCCGTCGCTTCGGTTGCGGATTTGCACCGTCGGGCTGGGATACGCTGACAAA
ACACCTGCAGCGCAAGGGTTTTGAGTTCAAGGAGCTGGAGGCGGCCGGCCTGTCGCGGCAGGGCCGGCGTGGCCCGATGG
ACCGGTTCCACCGGCGGCTGCTGTGGCCCATCCGCAGCTCGGCCGGTGAGGTGATCGGGTTCGGCGCCCGCCGGCTGTTC
GACGACGATCCGATGGAAGCCAAGTACATCAACACGCCCGAGACGCTGCTGTACAAGAAGTCGAACGTGATGTTCGGCAT
CGATCTGGCCAAACGCGACATCGCCAAGGGCCACCAGGCCGTCGTCGTCGAGGGCTACACCGACGTGATGGCGATGCATC
TGGCCGGGGTCACCACTGCGGTGGCCTCCTGCGGCACCGCCTTCGGCGACGAGCATCTGGCGATGCTGCGCCGACTCATG
ATGGACGACAGCTTCTTTCGCGGTGAACTGATCTACGTCTTCGACGGCGACGCGGCGGGGCGGGCCGCGGCGCTCAAGGC
GTTCGGCGGCGAGCAGAACCTGGCCGGGCAGTCCTTCGTCGCGGTCGCGCCGGACGGCATGGATCCCTGCGACCTGCGGC
TGCGCTCCGGCGACGCCGCGCTGCGCGACCTGGTGGCCCGGCGAACACCGTTGTTCGAGTTCGCAATCCGGTCGGCGCTC
GCCGAGCTGGACCTGGACAGCGCCGAGGGCAGGGTGGCCGCGCTGCGCCGCTGCGTGCCGATGGTGGCCCAGATCAAGGA
CCCCACGCTGCGCGACGAGTACGCCCGCCAGCTCGCCGGCTGGGTCGGCTGGAGCGACGTCGCGCAGGTCATCGACCGGG
TGCGCAGCCAGTCCAAGCACTCCGCCGGCGCGGGCCGCGGCGGCTCCGGCGCCAGGGTCTCCCGCCGCGCCGAGCAGTCC
GCCGCCCCGGCCGGTCCCGCCGCCAGCCGTCCCGACCCCCGCGATCCCACCCTGTGGCCGCAGCGGGAGGCACTCAAGTC
GGCGTTGCAGTACCCGGCGCTGGCCGGGCCGGTGTTCGACTCGCTGACCGTCGAGAGCTTCACCCATCCCGGGTACGCCG
CCGTGCGTGCCGCGATCGAGGCGGCCGGCGGCACGTCGAGCGGTGTCACCGGCGGGCAGTGGATCGAGGCGGTGCGCGAC
CGGGCCTCCTCGCCGCTGACGGCGGGGCTGATCAGCGAACTGGGTGTCGAGGCCATCCAGGTCGACGACGAGAAGCTGCC
GCGCTACATCGCCGGCGTGCTGGCCCGGCTGCAGGAGGTGTGGATGGGTCGGCAGATCGCCGAGGTGAAGTCGAAGCTGC
AGCGCATGTCGCCCATCGAGCAGGGCGACGAATACCACGCGCTGTTCGGGGACCTGGTGGCGATGGAGGCCTACCGGCGC
AGCCTGTTGGAGCAGGCTAGCGGGGACGATTAG

Upstream 100 bases:

>100_bases
CCGAGGACACCGAAAACGCGATGGACCGGGTTGCCTGCGGTGTCATCGGTACCGGATAGCTTTTCCGTACCCGGCGCGCT
GCGCGCCTTACACTGAGCCG

Downstream 100 bases:

>100_bases
ACGGCGCCAAGGCCTCGTCGGCCGGCTCGGCCTCGCGTTCCATGATGGCGGTCCGGTCGTCGATCTGCGTCAGCGTGACG
AACCCGGCGTCGGGCGAGAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 650; Mature: 649

Protein sequence:

>650_residues
MSSPAGSRAQGDGRARGRGRIPDRDIAAIRERVRIDEVVGDYVQLRRAGADSLKGLCPFHDEKSPSFHVRPNHGHFHCFG
CGEGGDVYAFLQKIEHVSFVEAVELLADRIGHTITYSGPATSVQRDRGSRSRLIAANAAAAEFYAAALESDEAAPARQYL
TERNFDAEAARRFGCGFAPSGWDTLTKHLQRKGFEFKELEAAGLSRQGRRGPMDRFHRRLLWPIRSSAGEVIGFGARRLF
DDDPMEAKYINTPETLLYKKSNVMFGIDLAKRDIAKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGDEHLAMLRRLM
MDDSFFRGELIYVFDGDAAGRAAALKAFGGEQNLAGQSFVAVAPDGMDPCDLRLRSGDAALRDLVARRTPLFEFAIRSAL
AELDLDSAEGRVAALRRCVPMVAQIKDPTLRDEYARQLAGWVGWSDVAQVIDRVRSQSKHSAGAGRGGSGARVSRRAEQS
AAPAGPAASRPDPRDPTLWPQREALKSALQYPALAGPVFDSLTVESFTHPGYAAVRAAIEAAGGTSSGVTGGQWIEAVRD
RASSPLTAGLISELGVEAIQVDDEKLPRYIAGVLARLQEVWMGRQIAEVKSKLQRMSPIEQGDEYHALFGDLVAMEAYRR
SLLEQASGDD

Sequences:

>Translated_650_residues
MSSPAGSRAQGDGRARGRGRIPDRDIAAIRERVRIDEVVGDYVQLRRAGADSLKGLCPFHDEKSPSFHVRPNHGHFHCFG
CGEGGDVYAFLQKIEHVSFVEAVELLADRIGHTITYSGPATSVQRDRGSRSRLIAANAAAAEFYAAALESDEAAPARQYL
TERNFDAEAARRFGCGFAPSGWDTLTKHLQRKGFEFKELEAAGLSRQGRRGPMDRFHRRLLWPIRSSAGEVIGFGARRLF
DDDPMEAKYINTPETLLYKKSNVMFGIDLAKRDIAKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGDEHLAMLRRLM
MDDSFFRGELIYVFDGDAAGRAAALKAFGGEQNLAGQSFVAVAPDGMDPCDLRLRSGDAALRDLVARRTPLFEFAIRSAL
AELDLDSAEGRVAALRRCVPMVAQIKDPTLRDEYARQLAGWVGWSDVAQVIDRVRSQSKHSAGAGRGGSGARVSRRAEQS
AAPAGPAASRPDPRDPTLWPQREALKSALQYPALAGPVFDSLTVESFTHPGYAAVRAAIEAAGGTSSGVTGGQWIEAVRD
RASSPLTAGLISELGVEAIQVDDEKLPRYIAGVLARLQEVWMGRQIAEVKSKLQRMSPIEQGDEYHALFGDLVAMEAYRR
SLLEQASGDD
>Mature_649_residues
SSPAGSRAQGDGRARGRGRIPDRDIAAIRERVRIDEVVGDYVQLRRAGADSLKGLCPFHDEKSPSFHVRPNHGHFHCFGC
GEGGDVYAFLQKIEHVSFVEAVELLADRIGHTITYSGPATSVQRDRGSRSRLIAANAAAAEFYAAALESDEAAPARQYLT
ERNFDAEAARRFGCGFAPSGWDTLTKHLQRKGFEFKELEAAGLSRQGRRGPMDRFHRRLLWPIRSSAGEVIGFGARRLFD
DDPMEAKYINTPETLLYKKSNVMFGIDLAKRDIAKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGDEHLAMLRRLMM
DDSFFRGELIYVFDGDAAGRAAALKAFGGEQNLAGQSFVAVAPDGMDPCDLRLRSGDAALRDLVARRTPLFEFAIRSALA
ELDLDSAEGRVAALRRCVPMVAQIKDPTLRDEYARQLAGWVGWSDVAQVIDRVRSQSKHSAGAGRGGSGARVSRRAEQSA
APAGPAASRPDPRDPTLWPQREALKSALQYPALAGPVFDSLTVESFTHPGYAAVRAAIEAAGGTSSGVTGGQWIEAVRDR
ASSPLTAGLISELGVEAIQVDDEKLPRYIAGVLARLQEVWMGRQIAEVKSKLQRMSPIEQGDEYHALFGDLVAMEAYRRS
LLEQASGDD

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=549, Percent_Identity=31.3296903460838, Blast_Score=237, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70602; Mature: 70471

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPAGSRAQGDGRARGRGRIPDRDIAAIRERVRIDEVVGDYVQLRRAGADSLKGLCPFH
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
DEKSPSFHVRPNHGHFHCFGCGEGGDVYAFLQKIEHVSFVEAVELLADRIGHTITYSGPA
CCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCC
TSVQRDRGSRSRLIAANAAAAEFYAAALESDEAAPARQYLTERNFDAEAARRFGCGFAPS
CHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCC
GWDTLTKHLQRKGFEFKELEAAGLSRQGRRGPMDRFHRRLLWPIRSSAGEVIGFGARRLF
CHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHC
DDDPMEAKYINTPETLLYKKSNVMFGIDLAKRDIAKGHQAVVVEGYTDVMAMHLAGVTTA
CCCCCCCEECCCCHHHEEECCCEEEEEHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHH
VASCGTAFGDEHLAMLRRLMMDDSFFRGELIYVFDGDAAGRAAALKAFGGEQNLAGQSFV
HHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCEE
AVAPDGMDPCDLRLRSGDAALRDLVARRTPLFEFAIRSALAELDLDSAEGRVAALRRCVP
EECCCCCCHHHEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
MVAQIKDPTLRDEYARQLAGWVGWSDVAQVIDRVRSQSKHSAGAGRGGSGARVSRRAEQS
HHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AAPAGPAASRPDPRDPTLWPQREALKSALQYPALAGPVFDSLTVESFTHPGYAAVRAAIE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
AAGGTSSGVTGGQWIEAVRDRASSPLTAGLISELGVEAIQVDDEKLPRYIAGVLARLQEV
HCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHH
WMGRQIAEVKSKLQRMSPIEQGDEYHALFGDLVAMEAYRRSLLEQASGDD
HHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSPAGSRAQGDGRARGRGRIPDRDIAAIRERVRIDEVVGDYVQLRRAGADSLKGLCPFH
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
DEKSPSFHVRPNHGHFHCFGCGEGGDVYAFLQKIEHVSFVEAVELLADRIGHTITYSGPA
CCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCC
TSVQRDRGSRSRLIAANAAAAEFYAAALESDEAAPARQYLTERNFDAEAARRFGCGFAPS
CHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCC
GWDTLTKHLQRKGFEFKELEAAGLSRQGRRGPMDRFHRRLLWPIRSSAGEVIGFGARRLF
CHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHC
DDDPMEAKYINTPETLLYKKSNVMFGIDLAKRDIAKGHQAVVVEGYTDVMAMHLAGVTTA
CCCCCCCEECCCCHHHEEECCCEEEEEHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHH
VASCGTAFGDEHLAMLRRLMMDDSFFRGELIYVFDGDAAGRAAALKAFGGEQNLAGQSFV
HHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCEE
AVAPDGMDPCDLRLRSGDAALRDLVARRTPLFEFAIRSALAELDLDSAEGRVAALRRCVP
EECCCCCCHHHEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
MVAQIKDPTLRDEYARQLAGWVGWSDVAQVIDRVRSQSKHSAGAGRGGSGARVSRRAEQS
HHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AAPAGPAASRPDPRDPTLWPQREALKSALQYPALAGPVFDSLTVESFTHPGYAAVRAAIE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
AAGGTSSGVTGGQWIEAVRDRASSPLTAGLISELGVEAIQVDDEKLPRYIAGVLARLQEV
HCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHH
WMGRQIAEVKSKLQRMSPIEQGDEYHALFGDLVAMEAYRRSLLEQASGDD
HHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]