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The map label for this gene is dlaT

Identifier: 41408054

GI number: 41408054

Start: 2159335

End: 2161107

Strand: Direct

Name: dlaT

Synonym: MAP1956

Alternate gene names: 41408054

Gene position: 2159335-2161107 (Clockwise)

Preceding gene: 41408051

Following gene: 41408055

Centisome position: 44.71

GC content: 72.36

Gene sequence:

>1773_bases
ATGGCCTTCTCCGTCCAGATGCCGGCACTCGGTGAGAGCGTCACCGAGGGGACGGTCACCCGCTGGCTCAAGCAGGAAGG
GGATACGGTCGAACTCGACGAGCCGCTGGTCGAGGTGTCCACCGACAAGGTCGACACCGAGATCCCGTCGCCTGCCGCGG
GTGTGCTGACCAAGATCATCGCGCAGGAAGACGACACCGTGGAGGTGGGCGGCGAACTGGCCGTGATCGGCGACGCGGCC
GACGGCGCCGCCGGCGGCGGGGCGCAGGCCCCCAGCCAGCCGAGCCAGCCCCAAGCACCGGCCCAGCCCGAGCCTCAGGC
CCAGGAACCGGCCCAGGCCCAGCCCGAGGCCCCGGCCCAGCCGCAGGCACCGGCCCAGCCGGCGCCACAGGAGTCCGGTG
GCGGCGCCGCCACCCCGGTGCTGATGCCCGAGCTGGGCGAGTCGGTGACCGAGGGCACCGTGACCCGCTGGCTCAAGAAG
GTCGGGGATTCGGTCCAGGTCGACGACGCGCTGGTCGAGGTGTCCACCGACAAGGTCGACACCGAGATCCCGTCGCCGGT
GGCCGGCGTGCTGATCAGCATCACGGCCGAGGAGGACAGCACCGTGCCGGTCGGCGGTGAGCTGGCCCGCATCGGCACCG
GCGCCCAGGCGCCCGCCCCCGCCGCGCCCCAACCCCCGCCGGCCCCCAAGCCCGAACCGAAACCCGAACCGGCACCCCAA
CCCCAGGCGCAGCCCGCGCCACAGCCACAAGCCCAGCCGGCGCCCGAGCCGAAAGCCCAACCGGCGCAAGCCCAGCCGGC
CGCCGGCGGCGAGGGCACCCCCTACGTGACACCGCTGGTGCGACGGCTGGCCGCCGAGAACGACATCGACCTGTCCTCGA
TCACCGGCACCGGGGTGGGCGGTCGCATCCGCAAGCAAGACGTCCTGGCCGCCGCCGAGCAGAAGCAGCGGCAACAACAG
GCCGCCGCCCAGCCGTCGGCCGCACCGGCCCCCGCGGCCGCCGAGGCCCGCAAGCCCGCCGCGCCGACGCCGGCGCCGGC
GCTGGCGCACCTGCGCGGCACCACCCAGAAGGCCAGCCGGATCCGTCAGATCACCGCGGCCAAGACGCGCGAATCCCTGC
TTGCCACAGCCCAACTCACCCAGACCCACGAGGTCGACATGACCAGGCTGGTCGGATTGCGGGCCCGGGCCAAGGCGGCG
TTCGCCGAGCGCGAGGGGGTGAACCTGACGTTCCTGCCGTTCATCGCCCGCGCGGTGATCGACGCGCTGAAGATCCACCC
CAACATCAACGCCAGTTACAACGAGGAAACCAAGGAGATCACCTACTACGACGCCGAGCACCTCGGCTTCGCGGTCGACA
CCGAACAGGGGCTGCTGTCCCCGGTCGTGCACAACGCCGGCGACCTGTCGCTGGCCGGGCTGGCCCGGGCGATCGCCGAC
ATCGCCGCGCGCGCCCGGTCGGGCAACCTCAAGCCCGACGAGCTATCCGGCGGCACCTTCACGATCACCAACATCGGCAG
CCAGGGCGCGCTGTTCGACACCCCGATCCTGGTCCCGCCGCAGGCCGCCATGCTGGGCACCGGGGCGATCGTCAAGCGGC
CGCGGGTGATCGTCGACGAGTTCGGCAACGAGTCGATCGGCGTGCGCTCGATCTGCTACCTGCCGCTGACCTACGACCAC
CGGCTGATCGACGGCGCCGACGCCGGCCGCTTCCTGACCACCATCAAGCACCGGCTCGAAGAGGGAGCATTCGAGGCCGA
CCTCGGGCTGTGA

Upstream 100 bases:

>100_bases
GCGGGCGCAGTGACAGGATAGTTTTGGATTGCGACTTCGCCTTCCGCGAGCGAGCTGATCGACCCGCCCCGACCCACGAG
CGATCGAGGAGTCAGAAGAG

Downstream 100 bases:

>100_bases
GAAGGGCGGTCGAACGTTGGCTCGCGCTGGGCAAAAGGCCGTCATCGCCATTGCGGGTTCGTCCGGCCTGATCGGCTCGG
CGCTGGCGGCGGCCCTGCGC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 590; Mature: 589

Protein sequence:

>590_residues
MAFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKIIAQEDDTVEVGGELAVIGDAA
DGAAGGGAQAPSQPSQPQAPAQPEPQAQEPAQAQPEAPAQPQAPAQPAPQESGGGAATPVLMPELGESVTEGTVTRWLKK
VGDSVQVDDALVEVSTDKVDTEIPSPVAGVLISITAEEDSTVPVGGELARIGTGAQAPAPAAPQPPPAPKPEPKPEPAPQ
PQAQPAPQPQAQPAPEPKAQPAQAQPAAGGEGTPYVTPLVRRLAAENDIDLSSITGTGVGGRIRKQDVLAAAEQKQRQQQ
AAAQPSAAPAPAAAEARKPAAPTPAPALAHLRGTTQKASRIRQITAAKTRESLLATAQLTQTHEVDMTRLVGLRARAKAA
FAEREGVNLTFLPFIARAVIDALKIHPNINASYNEETKEITYYDAEHLGFAVDTEQGLLSPVVHNAGDLSLAGLARAIAD
IAARARSGNLKPDELSGGTFTITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIVDEFGNESIGVRSICYLPLTYDH
RLIDGADAGRFLTTIKHRLEEGAFEADLGL

Sequences:

>Translated_590_residues
MAFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKIIAQEDDTVEVGGELAVIGDAA
DGAAGGGAQAPSQPSQPQAPAQPEPQAQEPAQAQPEAPAQPQAPAQPAPQESGGGAATPVLMPELGESVTEGTVTRWLKK
VGDSVQVDDALVEVSTDKVDTEIPSPVAGVLISITAEEDSTVPVGGELARIGTGAQAPAPAAPQPPPAPKPEPKPEPAPQ
PQAQPAPQPQAQPAPEPKAQPAQAQPAAGGEGTPYVTPLVRRLAAENDIDLSSITGTGVGGRIRKQDVLAAAEQKQRQQQ
AAAQPSAAPAPAAAEARKPAAPTPAPALAHLRGTTQKASRIRQITAAKTRESLLATAQLTQTHEVDMTRLVGLRARAKAA
FAEREGVNLTFLPFIARAVIDALKIHPNINASYNEETKEITYYDAEHLGFAVDTEQGLLSPVVHNAGDLSLAGLARAIAD
IAARARSGNLKPDELSGGTFTITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIVDEFGNESIGVRSICYLPLTYDH
RLIDGADAGRFLTTIKHRLEEGAFEADLGL
>Mature_589_residues
AFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKIIAQEDDTVEVGGELAVIGDAAD
GAAGGGAQAPSQPSQPQAPAQPEPQAQEPAQAQPEAPAQPQAPAQPAPQESGGGAATPVLMPELGESVTEGTVTRWLKKV
GDSVQVDDALVEVSTDKVDTEIPSPVAGVLISITAEEDSTVPVGGELARIGTGAQAPAPAAPQPPPAPKPEPKPEPAPQP
QAQPAPQPQAQPAPEPKAQPAQAQPAAGGEGTPYVTPLVRRLAAENDIDLSSITGTGVGGRIRKQDVLAAAEQKQRQQQA
AAQPSAAPAPAAAEARKPAAPTPAPALAHLRGTTQKASRIRQITAAKTRESLLATAQLTQTHEVDMTRLVGLRARAKAAF
AEREGVNLTFLPFIARAVIDALKIHPNINASYNEETKEITYYDAEHLGFAVDTEQGLLSPVVHNAGDLSLAGLARAIADI
AARARSGNLKPDELSGGTFTITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIVDEFGNESIGVRSICYLPLTYDHR
LIDGADAGRFLTTIKHRLEEGAFEADLGL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=457, Percent_Identity=31.0722100656455, Blast_Score=176, Evalue=7e-44,
Organism=Homo sapiens, GI31711992, Length=583, Percent_Identity=29.6740994854202, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI19923748, Length=247, Percent_Identity=38.8663967611336, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI203098816, Length=471, Percent_Identity=27.8131634819533, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI203098753, Length=465, Percent_Identity=27.741935483871, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=32.0512820512821, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786946, Length=450, Percent_Identity=34.6666666666667, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1786305, Length=583, Percent_Identity=27.958833619211, Blast_Score=166, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=441, Percent_Identity=30.6122448979592, Blast_Score=178, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=459, Percent_Identity=28.5403050108932, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=466, Percent_Identity=29.8283261802575, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=326, Percent_Identity=26.0736196319018, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320352, Length=474, Percent_Identity=30.5907172995781, Blast_Score=193, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=27.4336283185841, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859875, Length=448, Percent_Identity=28.125, Blast_Score=152, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=36, Blast_Score=140, Evalue=4e-33,
Organism=Drosophila melanogaster, GI20129315, Length=449, Percent_Identity=27.3942093541203, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24582497, Length=439, Percent_Identity=27.5626423690205, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR014276
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 61163; Mature: 61032

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKII
CCEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHECCCCCCCCCCCCCHHHHHHHHH
AQEDDTVEVGGELAVIGDAADGAAGGGAQAPSQPSQPQAPAQPEPQAQEPAQAQPEAPAQ
HCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PQAPAQPAPQESGGGAATPVLMPELGESVTEGTVTRWLKKVGDSVQVDDALVEVSTDKVD
CCCCCCCCCCCCCCCCCCCEECHHHCCHHHHHHHHHHHHHHCCCEEECHHEEEEECCCCC
TEIPSPVAGVLISITAEEDSTVPVGGELARIGTGAQAPAPAAPQPPPAPKPEPKPEPAPQ
CCCCCCCCCEEEEEECCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PQAQPAPQPQAQPAPEPKAQPAQAQPAAGGEGTPYVTPLVRRLAAENDIDLSSITGTGVG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCCCCCC
GRIRKQDVLAAAEQKQRQQQAAAQPSAAPAPAAAEARKPAAPTPAPALAHLRGTTQKASR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCHHHHHH
IRQITAAKTRESLLATAQLTQTHEVDMTRLVGLRARAKAAFAEREGVNLTFLPFIARAVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHH
DALKIHPNINASYNEETKEITYYDAEHLGFAVDTEQGLLSPVVHNAGDLSLAGLARAIAD
HHHHCCCCCCCCCCCCCCEEEEEEHHHCCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHH
IAARARSGNLKPDELSGGTFTITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIVDE
HHHHHHCCCCCCCCCCCCEEEEEECCCCCCEECCCEECCCCHHHHCCCHHHCCCCHHHHH
FGNESIGVRSICYLPLTYDHRLIDGADAGRFLTTIKHRLEEGAFEADLGL
CCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
AFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKII
CEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHECCCCCCCCCCCCCHHHHHHHHH
AQEDDTVEVGGELAVIGDAADGAAGGGAQAPSQPSQPQAPAQPEPQAQEPAQAQPEAPAQ
HCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PQAPAQPAPQESGGGAATPVLMPELGESVTEGTVTRWLKKVGDSVQVDDALVEVSTDKVD
CCCCCCCCCCCCCCCCCCCEECHHHCCHHHHHHHHHHHHHHCCCEEECHHEEEEECCCCC
TEIPSPVAGVLISITAEEDSTVPVGGELARIGTGAQAPAPAAPQPPPAPKPEPKPEPAPQ
CCCCCCCCCEEEEEECCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PQAQPAPQPQAQPAPEPKAQPAQAQPAAGGEGTPYVTPLVRRLAAENDIDLSSITGTGVG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCCCCCC
GRIRKQDVLAAAEQKQRQQQAAAQPSAAPAPAAAEARKPAAPTPAPALAHLRGTTQKASR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCHHHHHH
IRQITAAKTRESLLATAQLTQTHEVDMTRLVGLRARAKAAFAEREGVNLTFLPFIARAVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHH
DALKIHPNINASYNEETKEITYYDAEHLGFAVDTEQGLLSPVVHNAGDLSLAGLARAIAD
HHHHCCCCCCCCCCCCCCEEEEEEHHHCCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHH
IAARARSGNLKPDELSGGTFTITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIVDE
HHHHHHCCCCCCCCCCCCEEEEEECCCCCCEECCCEECCCCHHHHCCCHHHCCCCHHHHH
FGNESIGVRSICYLPLTYDHRLIDGADAGRFLTTIKHRLEEGAFEADLGL
CCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]