Click here to switch to the map view.

The map label for this gene is carA

Identifier: 41407216

GI number: 41407216

Start: 1170006

End: 1171127

Strand: Direct

Name: carA

Synonym: MAP1118

Alternate gene names: 41407216

Gene position: 1170006-1171127 (Clockwise)

Preceding gene: 41407215

Following gene: 41407217

Centisome position: 24.22

GC content: 69.43

Gene sequence:

>1122_bases
GTGAGTGGCAAGGCGCAACTGGTGCTCGAGGACGGCCGCGTCTTCACCGGCACGGCGTTCGGAGCGATCGGCCAAACCCT
GGGCGAGGCCGTGTTTTCCACCGGCATGTCCGGCTATCAGGAGACGCTGACGGACCCCAGCTACCACCGCCAGATCGTGG
TGGCCACCGCGCCGCAGATCGGCAATACCGGCTGGAACGGCGAGGACGCCGAGAGCCGCGGCGACAAGATCTGGGTGGCC
GGCTACGCGGTGCGCGACCCGTCGCCGCGCGTGTCCAACTGGCGCGCCACCGGAAGCCTGGAGGACGAGCTGATCCGCCA
GCGCATCGTGGGCATCGCTCGCATCGACACCCGCGCGGTGGTGCGCCACCTGCGGACCCGCGGTTCGATGAAGGCGGGGG
TGTTCTCCGGTGACGCCCTGGCCGATCCGGACGAACTGGTGCAGCGGGTGCGCGGCCAGCAGTCCATGCTGGGCGCCGAC
CTGGCCGGTGAGGTCAGCACGCCGGACGCCTACATTGTGGAACCCGAAGGGCCGCCGCGGTTTACCGTCGCCGCACTGGA
CCTGGGCATCAAAACCAACACGCCGCGCAACTTCGCCCGTCGCGGCATCCGCAGCCACGTGCTGCCCTCGTCGGCGACCT
TCGAGCAGATCGCCGACCTCAGGCCGGACGGCGTGTTCCTGTCCAACGGGCCCGGCGACCCCGCCACCGCCGACCACATC
GTGGCGGTCACCCGCGAGGTGCTGGGCGCCGGAATCCCATTGTTCGGCATCTGTTTCGGCAACCAGATCCTGGGCCGGGC
GCTGGGCCTGTCCACCTACAAGATGGTGTTCGGTCACCGCGGCATCAACATTCCGGTGATCGACCACGCCACCGGGCGGG
TGGCGGTGACCGCGCAGAACCACGGCTTCGCGCTGCAGGGCGAGGCCGGCCAGTCCTTCGACACGCCCTTCGGCGCGGCG
GTGGTCAGCCACACCTGCGCCAACGACGGGGTGGTGGAGGGCGTGAAACTCGCTGACGGACGGGCATTTTCGGTGCAGTA
CCACCCGGAGGCGGCCGCCGGCCCGCACGACGCGAACTACCTCTTCGACCAATTCATCGAGCTGATGGAAGGGGACCGCT
AG

Upstream 100 bases:

>100_bases
GGCGGCTGCCGTCGGGCGTGGAGATCGACACCGGCTTCCGGGCCAACAACCGGGGCGAATACGACGGCTGGCTGCAGCCG
GGGCGGGAGGAGCGGCGTTC

Downstream 100 bases:

>100_bases
TGCCCCGGCGCACCGACCTCAACCACGTCCTGGTGATCGGCTCGGGCCCGATCGTGATCGGGCAGGCCTGCGAGTTCGAC
TATTCCGGCACCCAGGCCTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSGKAQLVLEDGRVFTGTAFGAIGQTLGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIGNTGWNGEDAESRGDKIWVA
GYAVRDPSPRVSNWRATGSLEDELIRQRIVGIARIDTRAVVRHLRTRGSMKAGVFSGDALADPDELVQRVRGQQSMLGAD
LAGEVSTPDAYIVEPEGPPRFTVAALDLGIKTNTPRNFARRGIRSHVLPSSATFEQIADLRPDGVFLSNGPGDPATADHI
VAVTREVLGAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVIDHATGRVAVTAQNHGFALQGEAGQSFDTPFGAA
VVSHTCANDGVVEGVKLADGRAFSVQYHPEAAAGPHDANYLFDQFIELMEGDR

Sequences:

>Translated_373_residues
MSGKAQLVLEDGRVFTGTAFGAIGQTLGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIGNTGWNGEDAESRGDKIWVA
GYAVRDPSPRVSNWRATGSLEDELIRQRIVGIARIDTRAVVRHLRTRGSMKAGVFSGDALADPDELVQRVRGQQSMLGAD
LAGEVSTPDAYIVEPEGPPRFTVAALDLGIKTNTPRNFARRGIRSHVLPSSATFEQIADLRPDGVFLSNGPGDPATADHI
VAVTREVLGAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVIDHATGRVAVTAQNHGFALQGEAGQSFDTPFGAA
VVSHTCANDGVVEGVKLADGRAFSVQYHPEAAAGPHDANYLFDQFIELMEGDR
>Mature_372_residues
SGKAQLVLEDGRVFTGTAFGAIGQTLGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIGNTGWNGEDAESRGDKIWVAG
YAVRDPSPRVSNWRATGSLEDELIRQRIVGIARIDTRAVVRHLRTRGSMKAGVFSGDALADPDELVQRVRGQQSMLGADL
AGEVSTPDAYIVEPEGPPRFTVAALDLGIKTNTPRNFARRGIRSHVLPSSATFEQIADLRPDGVFLSNGPGDPATADHIV
AVTREVLGAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVIDHATGRVAVTAQNHGFALQGEAGQSFDTPFGAAV
VSHTCANDGVVEGVKLADGRAFSVQYHPEAAAGPHDANYLFDQFIELMEGDR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=380, Percent_Identity=38.9473684210526, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI169790915, Length=381, Percent_Identity=35.1706036745407, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI21361331, Length=381, Percent_Identity=35.1706036745407, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI1786215, Length=389, Percent_Identity=43.9588688946015, Blast_Score=287, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI193204318, Length=375, Percent_Identity=38.1333333333333, Blast_Score=224, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6324878, Length=380, Percent_Identity=35, Blast_Score=209, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6322331, Length=393, Percent_Identity=35.8778625954198, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45555749, Length=397, Percent_Identity=38.7909319899244, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24642586, Length=397, Percent_Identity=38.7909319899244, Blast_Score=231, Evalue=4e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_MYCPA (Q741H2)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_960052.1
- ProteinModelPortal:   Q741H2
- SMR:   Q741H2
- EnsemblBacteria:   EBMYCT00000039900
- GeneID:   2718934
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP1118
- NMPDR:   fig|262316.1.peg.1118
- GeneTree:   EBGT00050000017681
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 39693; Mature: 39562

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 258-258 ACT_SITE 348-348 ACT_SITE 350-350

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKAQLVLEDGRVFTGTAFGAIGQTLGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQI
CCCCEEEEEECCEEEECHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCC
GNTGWNGEDAESRGDKIWVAGYAVRDPSPRVSNWRATGSLEDELIRQRIVGIARIDTRAV
CCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCHHHH
VRHLRTRGSMKAGVFSGDALADPDELVQRVRGQQSMLGADLAGEVSTPDAYIVEPEGPPR
HHHHHHCCCCCCCEECCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEECCCCCCC
FTVAALDLGIKTNTPRNFARRGIRSHVLPSSATFEQIADLRPDGVFLSNGPGDPATADHI
EEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCCCCHHHH
VAVTREVLGAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVIDHATGRVAVTAQN
HHHHHHHHHCCCCEEEEHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECCCCEEEEEECC
HGFALQGEAGQSFDTPFGAAVVSHTCANDGVVEGVKLADGRAFSVQYHPEAAAGPHDANY
CCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCHHH
LFDQFIELMEGDR
HHHHHHHHHCCCC
>Mature Secondary Structure 
SGKAQLVLEDGRVFTGTAFGAIGQTLGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQI
CCCEEEEEECCEEEECHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCC
GNTGWNGEDAESRGDKIWVAGYAVRDPSPRVSNWRATGSLEDELIRQRIVGIARIDTRAV
CCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCHHHH
VRHLRTRGSMKAGVFSGDALADPDELVQRVRGQQSMLGADLAGEVSTPDAYIVEPEGPPR
HHHHHHCCCCCCCEECCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEECCCCCCC
FTVAALDLGIKTNTPRNFARRGIRSHVLPSSATFEQIADLRPDGVFLSNGPGDPATADHI
EEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCCCCHHHH
VAVTREVLGAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVIDHATGRVAVTAQN
HHHHHHHHHCCCCEEEEHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECCCCEEEEEECC
HGFALQGEAGQSFDTPFGAAVVSHTCANDGVVEGVKLADGRAFSVQYHPEAAAGPHDANY
CCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCHHH
LFDQFIELMEGDR
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA