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The map label for this gene is nikA [H]

Identifier: 41407184

GI number: 41407184

Start: 1139575

End: 1141167

Strand: Direct

Name: nikA [H]

Synonym: MAP1086

Alternate gene names: 41407184

Gene position: 1139575-1141167 (Clockwise)

Preceding gene: 41407183

Following gene: 41407185

Centisome position: 23.59

GC content: 67.36

Gene sequence:

>1593_bases
ATGGCGAAGGTAGCGACGCGCACCGCGACGCTGGGCGCGGTGCTGTGCGTCCTGGCGGCGGGGTGTTCCGGCCATCGGCA
CACCGCGAGCACCGGACCCACGGGTCCGCCGGTGCGCGGCGGCACCCTGACCTACTCGGACGTGCAGTTCGTCACCGACA
CCATGGCCGCCAACTACAGCGCCAACAACCTGATGTTCCAGCTGCTCGACCGGGTGGTCTACGTCGACCCGAAAACCGAC
GCGGTGCGCGGGTGGCTGGCGAAAACCTTCTCCCGCAACCAGAACGCCACCCGGTACACCTTCACCATCCGCGACGGGGT
GAGCTTCAGCGACCACACACCGCTGACCGCCGAGGTGGTCAAGGCGAATTTCGACCAGCTCGGCAAGGGTGACCCGGCCA
AGAAGATGCCGGCCTTCGCCGATTTCGTCGGCTACGACCACAGCGAGGTCAGCGGCAACGTCGTCACCGTTTTCCTCGAC
CGGCCCAACACCAACTTCTACAAGGTGCTGTCCTACAGCCGCGCGGGCATCCGCGCACCGGCCACGCTGGCGCTCGACTA
CCAGCGCCAGGCCAAGGTTGAAAACGTCATCGGCTCAGGCCCTTTCGTGTACCAGTCGCAGATCCCCGACCAGCAGGTGG
TGCTCACCAGGCGCCCGGACTACGCGTGGCCGCCGTCGTCGTGGCCCAACCAGGGGCCGGCCTACGTCGACAAGGTCGTC
TTCCGGGTGATCGGCGAGCCGGGCCTGCGCGCGGGCGCGGTCCAATCCCATCAGGTGGACGTGGCCCGCGGAATCCAGCC
CACCGACGAACCCGCGCTCAAACAGGGTGGCCTGCAGGTGATCCCGGTGGCGGCTCCCGCCGAGACCGCGAACCTGGTCG
GGTTCCGGATCAACAACCAGGTCGTCGACGACGTCCGGGTGCGCCGGGCGCTGCAACTGGGCATCGACCGTCGGGCGGTG
GTGGACACGGTGCTCTCCGACAGCTATCGCGCGGCCGATTCCGTGCTGGGACACGACGATCCGTTGTTCGCCGATGTGAG
CGCCGACATCGGGTATCAGCCGCAGCGGGCGGCCGGCCTGCTCGATGCGGCCGGGTGGAAGCCGGGCGGCGACGGCGTGC
GCGAAAAGGACGGCAAGAAACTTGTTCTGACGCTGGCGGCGTCCAACCAGAGCGTGGTGTTCCGCCCGGCGTTCGAGTTC
ATCGAACAGCAGTGGCGGGAGCTGGGCGTGGTGCTGCAGAACCGGGCGGGCGACACGACGTTCTTCAATGCCTCCAGCAG
TCAATCGTCAACCCCGTTGTTCGGCACCAGGATTCAGTACAACATCGGGCTGGGCCTGCTGTTCGGCCGCCCGAAAAGCA
ACCTGACGTTCGCGCAGAACGACGCGTTGCTCGCGCTGTCCGAGGAGGAACTGCGGCAGACCGACCCGACGCGGCTCAAA
GACGTCGTCGCCCGCGAGCAGCGCAGGATCATCGACGACCAGCTGGCGTTGGTGCTGTGGGATGAGGTGCAGGTGCACGC
CGCCGCACCCAACGTGCACGTGGAATTCACCCGCTACACCGAGCCGCTGCTGCAGAGCGCCTGGAAGGCGTGA

Upstream 100 bases:

>100_bases
GTTCTCGCACAACCCGACGGTCTACAAGGCGCGGGTGCTCGGCGATGTCGCCGTCAACGGCGCCGCCCTGCCCGACACCA
GCTTCTTCTGACCCGCTTGC

Downstream 100 bases:

>100_bases
GGGGGATGCTCGGCTACGTTCTCGCCCGGATCGGCCAGTCGGCCATCGTGCTGCTGGCGGTCTTCAGCCTGGTGTTCTGG
GGCGTCAGCATCCTGCCGGC

Product: hypothetical protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MAKVATRTATLGAVLCVLAAGCSGHRHTASTGPTGPPVRGGTLTYSDVQFVTDTMAANYSANNLMFQLLDRVVYVDPKTD
AVRGWLAKTFSRNQNATRYTFTIRDGVSFSDHTPLTAEVVKANFDQLGKGDPAKKMPAFADFVGYDHSEVSGNVVTVFLD
RPNTNFYKVLSYSRAGIRAPATLALDYQRQAKVENVIGSGPFVYQSQIPDQQVVLTRRPDYAWPPSSWPNQGPAYVDKVV
FRVIGEPGLRAGAVQSHQVDVARGIQPTDEPALKQGGLQVIPVAAPAETANLVGFRINNQVVDDVRVRRALQLGIDRRAV
VDTVLSDSYRAADSVLGHDDPLFADVSADIGYQPQRAAGLLDAAGWKPGGDGVREKDGKKLVLTLAASNQSVVFRPAFEF
IEQQWRELGVVLQNRAGDTTFFNASSSQSSTPLFGTRIQYNIGLGLLFGRPKSNLTFAQNDALLALSEEELRQTDPTRLK
DVVAREQRRIIDDQLALVLWDEVQVHAAAPNVHVEFTRYTEPLLQSAWKA

Sequences:

>Translated_530_residues
MAKVATRTATLGAVLCVLAAGCSGHRHTASTGPTGPPVRGGTLTYSDVQFVTDTMAANYSANNLMFQLLDRVVYVDPKTD
AVRGWLAKTFSRNQNATRYTFTIRDGVSFSDHTPLTAEVVKANFDQLGKGDPAKKMPAFADFVGYDHSEVSGNVVTVFLD
RPNTNFYKVLSYSRAGIRAPATLALDYQRQAKVENVIGSGPFVYQSQIPDQQVVLTRRPDYAWPPSSWPNQGPAYVDKVV
FRVIGEPGLRAGAVQSHQVDVARGIQPTDEPALKQGGLQVIPVAAPAETANLVGFRINNQVVDDVRVRRALQLGIDRRAV
VDTVLSDSYRAADSVLGHDDPLFADVSADIGYQPQRAAGLLDAAGWKPGGDGVREKDGKKLVLTLAASNQSVVFRPAFEF
IEQQWRELGVVLQNRAGDTTFFNASSSQSSTPLFGTRIQYNIGLGLLFGRPKSNLTFAQNDALLALSEEELRQTDPTRLK
DVVAREQRRIIDDQLALVLWDEVQVHAAAPNVHVEFTRYTEPLLQSAWKA
>Mature_529_residues
AKVATRTATLGAVLCVLAAGCSGHRHTASTGPTGPPVRGGTLTYSDVQFVTDTMAANYSANNLMFQLLDRVVYVDPKTDA
VRGWLAKTFSRNQNATRYTFTIRDGVSFSDHTPLTAEVVKANFDQLGKGDPAKKMPAFADFVGYDHSEVSGNVVTVFLDR
PNTNFYKVLSYSRAGIRAPATLALDYQRQAKVENVIGSGPFVYQSQIPDQQVVLTRRPDYAWPPSSWPNQGPAYVDKVVF
RVIGEPGLRAGAVQSHQVDVARGIQPTDEPALKQGGLQVIPVAAPAETANLVGFRINNQVVDDVRVRRALQLGIDRRAVV
DTVLSDSYRAADSVLGHDDPLFADVSADIGYQPQRAAGLLDAAGWKPGGDGVREKDGKKLVLTLAASNQSVVFRPAFEFI
EQQWRELGVVLQNRAGDTTFFNASSSQSSTPLFGTRIQYNIGLGLLFGRPKSNLTFAQNDALLALSEEELRQTDPTRLKD
VVAREQRRIIDDQLALVLWDEVQVHAAAPNVHVEFTRYTEPLLQSAWKA

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=363, Percent_Identity=25.6198347107438, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787762, Length=252, Percent_Identity=30.1587301587302, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787052, Length=366, Percent_Identity=23.4972677595628, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789966, Length=328, Percent_Identity=24.390243902439, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787495, Length=254, Percent_Identity=23.6220472440945, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57899; Mature: 57768

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVATRTATLGAVLCVLAAGCSGHRHTASTGPTGPPVRGGTLTYSDVQFVTDTMAANYS
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCC
ANNLMFQLLDRVVYVDPKTDAVRGWLAKTFSRNQNATRYTFTIRDGVSFSDHTPLTAEVV
HHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHH
KANFDQLGKGDPAKKMPAFADFVGYDHSEVSGNVVTVFLDRPNTNFYKVLSYSRAGIRAP
HCCHHHCCCCCCHHHCCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCC
ATLALDYQRQAKVENVIGSGPFVYQSQIPDQQVVLTRRPDYAWPPSSWPNQGPAYVDKVV
EEEEECCHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
FRVIGEPGLRAGAVQSHQVDVARGIQPTDEPALKQGGLQVIPVAAPAETANLVGFRINNQ
HHHHCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHCCEEEEEECCCCCCCEEEEEEECCH
VVDDVRVRRALQLGIDRRAVVDTVLSDSYRAADSVLGHDDPLFADVSADIGYQPQRAAGL
HHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCCCCCCHHHHCC
LDAAGWKPGGDGVREKDGKKLVLTLAASNQSVVFRPAFEFIEQQWRELGVVLQNRAGDTT
HHCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCE
FFNASSSQSSTPLFGTRIQYNIGLGLLFGRPKSNLTFAQNDALLALSEEELRQTDPTRLK
EEECCCCCCCCCEEEEEEEEEECEEEEECCCCCCCEEECCCEEEEECHHHHHCCCHHHHH
DVVAREQRRIIDDQLALVLWDEVQVHAAAPNVHVEFTRYTEPLLQSAWKA
HHHHHHHHHHHHHHEEEEEEEEEEEEEECCCEEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKVATRTATLGAVLCVLAAGCSGHRHTASTGPTGPPVRGGTLTYSDVQFVTDTMAANYS
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCC
ANNLMFQLLDRVVYVDPKTDAVRGWLAKTFSRNQNATRYTFTIRDGVSFSDHTPLTAEVV
HHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHH
KANFDQLGKGDPAKKMPAFADFVGYDHSEVSGNVVTVFLDRPNTNFYKVLSYSRAGIRAP
HCCHHHCCCCCCHHHCCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCC
ATLALDYQRQAKVENVIGSGPFVYQSQIPDQQVVLTRRPDYAWPPSSWPNQGPAYVDKVV
EEEEECCHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
FRVIGEPGLRAGAVQSHQVDVARGIQPTDEPALKQGGLQVIPVAAPAETANLVGFRINNQ
HHHHCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHCCEEEEEECCCCCCCEEEEEEECCH
VVDDVRVRRALQLGIDRRAVVDTVLSDSYRAADSVLGHDDPLFADVSADIGYQPQRAAGL
HHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCCCCCCHHHHCC
LDAAGWKPGGDGVREKDGKKLVLTLAASNQSVVFRPAFEFIEQQWRELGVVLQNRAGDTT
HHCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCE
FFNASSSQSSTPLFGTRIQYNIGLGLLFGRPKSNLTFAQNDALLALSEEELRQTDPTRLK
EEECCCCCCCCCEEEEEEEEEECEEEEECCCCCCCEEECCCEEEEECHHHHHCCCHHHHH
DVVAREQRRIIDDQLALVLWDEVQVHAAAPNVHVEFTRYTEPLLQSAWKA
HHHHHHHHHHHHHHEEEEEEEEEEEEEECCCEEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]