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The map label for this gene is paaF [C]

Identifier: 41406781

GI number: 41406781

Start: 703972

End: 704736

Strand: Direct

Name: paaF [C]

Synonym: MAP0683

Alternate gene names: 41406781

Gene position: 703972-704736 (Clockwise)

Preceding gene: 41406780

Following gene: 41406783

Centisome position: 14.58

GC content: 67.06

Gene sequence:

>765_bases
GTGTACGACATGCCTACCGAAATCGATGTCCGGGCCGACGGTGCGCTGCGCATCATCACGCTGAACCGGCCGGACTCGCT
CAACTCGGTCAACGACGACCTGCACGTGGGGCTGGCGCGGCTGTGGCAGCGGTTGACCGATGACCCCACCGCGCGTGCCG
CCGTGATCACCGGTGCCGGCCGGGCTTTTTCGGCGGGCGGCGATTTCGGGTATCTCAAGGAGCTGTCCGCCGACGCCGAC
CTGCGCGCCAAGACGATCCGGGACGGGCGCGAGATCGTGCTGGGCATGGCGCGCTGCCGGATTCCGGTGGTAGCGGCCGT
CAATGGCCCCGCGGTAGGGCTGGGCTGCAGCCTGGTGGCGCTGAGCGACATCGTCTACATCGCCGAGAACGCCTACCTGG
CCGACCCGCATGTGCAGGTGGGTCTGGTGGCCGCCGACGGTGGGCCGTTGACCTGGCCGCTGCACATCAGCCTGTTGCTG
GCCAAGGAGTACGCGCTGACCGGCACCCGGATCAGCGCGCAGCGCGCCGTCGAGCTCGGGTTGGCCAATCACGTGGCCGA
CGATCCCGTCGCCGAGGCAATCGCCTGCGCCAAAAAGATTTTGGAGCTGCCGCAGCAGGCGGTGGAAAGCACCAAGCGGG
TGCTCAACATCCATCTGGAGCGGGCCGTGCTGGCCAGTCTGGACTATGCGTTGTCGGCCGAGAGCCAGTCGTTCGTCACC
GAGGATTTCCGGTCGATCGTCACCAAACTGGCGGACAAGAACTGA

Upstream 100 bases:

>100_bases
AAGCCCGGGTGAAGCGGGTCACGACCATTAAACAAGGAGTGATCGCGAGCGCGGGCGAAGCCCGGGTGAAGCGGGTCACG
ACCATCTAAAGGAGTCTGAC

Downstream 100 bases:

>100_bases
ATCGATCAGTCGTGCAGCCGTAAGAAGTCACGCATCACCGCGTCGAACTTCTCCGGTTCCTCGATGAACGGCATGTGGGC
GCTGGACTCGAACAGCTCGA

Product: hypothetical protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MYDMPTEIDVRADGALRIITLNRPDSLNSVNDDLHVGLARLWQRLTDDPTARAAVITGAGRAFSAGGDFGYLKELSADAD
LRAKTIRDGREIVLGMARCRIPVVAAVNGPAVGLGCSLVALSDIVYIAENAYLADPHVQVGLVAADGGPLTWPLHISLLL
AKEYALTGTRISAQRAVELGLANHVADDPVAEAIACAKKILELPQQAVESTKRVLNIHLERAVLASLDYALSAESQSFVT
EDFRSIVTKLADKN

Sequences:

>Translated_254_residues
MYDMPTEIDVRADGALRIITLNRPDSLNSVNDDLHVGLARLWQRLTDDPTARAAVITGAGRAFSAGGDFGYLKELSADAD
LRAKTIRDGREIVLGMARCRIPVVAAVNGPAVGLGCSLVALSDIVYIAENAYLADPHVQVGLVAADGGPLTWPLHISLLL
AKEYALTGTRISAQRAVELGLANHVADDPVAEAIACAKKILELPQQAVESTKRVLNIHLERAVLASLDYALSAESQSFVT
EDFRSIVTKLADKN
>Mature_254_residues
MYDMPTEIDVRADGALRIITLNRPDSLNSVNDDLHVGLARLWQRLTDDPTARAAVITGAGRAFSAGGDFGYLKELSADAD
LRAKTIRDGREIVLGMARCRIPVVAAVNGPAVGLGCSLVALSDIVYIAENAYLADPHVQVGLVAADGGPLTWPLHISLLL
AKEYALTGTRISAQRAVELGLANHVADDPVAEAIACAKKILELPQQAVESTKRVLNIHLERAVLASLDYALSAESQSFVT
EDFRSIVTKLADKN

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=238, Percent_Identity=32.3529411764706, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI70995211, Length=238, Percent_Identity=30.672268907563, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI116235487, Length=142, Percent_Identity=33.0985915492958, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4757968, Length=178, Percent_Identity=27.5280898876405, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI49355787, Length=178, Percent_Identity=27.5280898876405, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI25453481, Length=178, Percent_Identity=27.5280898876405, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI51538573, Length=178, Percent_Identity=27.5280898876405, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4757966, Length=178, Percent_Identity=27.5280898876405, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI51538581, Length=178, Percent_Identity=27.5280898876405, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI45643119, Length=208, Percent_Identity=27.4038461538462, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260275230, Length=208, Percent_Identity=27.4038461538462, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260274832, Length=208, Percent_Identity=27.4038461538462, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594469, Length=225, Percent_Identity=27.5555555555556, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI37594471, Length=225, Percent_Identity=27.5555555555556, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221307494, Length=178, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI221136756, Length=178, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI221136753, Length=178, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI31542718, Length=221, Percent_Identity=28.0542986425339, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=30.5882352941176, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI221142681, Length=209, Percent_Identity=33.9712918660287, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787660, Length=175, Percent_Identity=36.5714285714286, Blast_Score=74, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=33.4710743801653, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=240, Percent_Identity=31.6666666666667, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17535521, Length=226, Percent_Identity=29.646017699115, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=253, Percent_Identity=26.4822134387352, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=228, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=237, Percent_Identity=26.1603375527426, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=246, Percent_Identity=25.609756097561, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=218, Percent_Identity=26.605504587156, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323314, Length=213, Percent_Identity=26.7605633802817, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324754, Length=180, Percent_Identity=28.8888888888889, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129971, Length=238, Percent_Identity=31.9327731092437, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24653477, Length=238, Percent_Identity=31.9327731092437, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24654903, Length=219, Percent_Identity=28.310502283105, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19920382, Length=243, Percent_Identity=27.1604938271605, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19922422, Length=139, Percent_Identity=31.6546762589928, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21357171, Length=213, Percent_Identity=27.6995305164319, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27134; Mature: 27134

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDMPTEIDVRADGALRIITLNRPDSLNSVNDDLHVGLARLWQRLTDDPTARAAVITGAG
CCCCCCCEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
RAFSAGGDFGYLKELSADADLRAKTIRDGREIVLGMARCRIPVVAAVNGPAVGLGCSLVA
CEECCCCCCHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCEEEEEEECCCCCCCCHHHHH
LSDIVYIAENAYLADPHVQVGLVAADGGPLTWPLHISLLLAKEYALTGTRISAQRAVELG
HHHHHHEECCCEEECCCEEEEEEEECCCCEEEHHHHEEEEHHHHHHCCCCHHHHHHHHHH
LANHVADDPVAEAIACAKKILELPQQAVESTKRVLNIHLERAVLASLDYALSAESQSFVT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EDFRSIVTKLADKN
HHHHHHHHHHCCCC
>Mature Secondary Structure
MYDMPTEIDVRADGALRIITLNRPDSLNSVNDDLHVGLARLWQRLTDDPTARAAVITGAG
CCCCCCCEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
RAFSAGGDFGYLKELSADADLRAKTIRDGREIVLGMARCRIPVVAAVNGPAVGLGCSLVA
CEECCCCCCHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCEEEEEEECCCCCCCCHHHHH
LSDIVYIAENAYLADPHVQVGLVAADGGPLTWPLHISLLLAKEYALTGTRISAQRAVELG
HHHHHHEECCCEEECCCEEEEEEEECCCCEEEHHHHEEEEHHHHHHCCCCHHHHHHHHHH
LANHVADDPVAEAIACAKKILELPQQAVESTKRVLNIHLERAVLASLDYALSAESQSFVT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EDFRSIVTKLADKN
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA