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The map label for this gene is purB

Identifier: 41406709

GI number: 41406709

Start: 635803

End: 637221

Strand: Direct

Name: purB

Synonym: MAP0611

Alternate gene names: 41406709

Gene position: 635803-637221 (Clockwise)

Preceding gene: 41406707

Following gene: 41406710

Centisome position: 13.16

GC content: 69.98

Gene sequence:

>1419_bases
GTGAGCATTCCGAATGTGCTGGCTGCCCGGTACGCCAGTGCCGAGATGGTTGCGATCTGGTCGCCGGAAGCCAAAGTGGT
CGCCGAGCGGCGGCTGTGGCTCGCGGTGCTGCGCGCGCAGCAACAGTTGGGTGTCGCGGTTCCCGCCGAGGCGATCGCGG
ACTACGAGCGGGTACTCGAGGACGTCGATCTGGCCTCGATCGCGGACCGCGAACGCCTGCTGCGCCACGACGTCAAGGCC
CGCATCGAGGAATTCAACGCGCTGGCCGGCCACGAGCACGTGCACAAGGGCATGACCAGCCGGGATCTGACCGAGAACGT
CGAGCAGCTGCAGATCCGGCGCTCCCTGGAACTGGTCTTCGCCCACGGCGTCGCGGTCGCCGCTCGGCTGGCCGAACGGG
CGGTGGCCTACCGCGATCTGGTGATGGCCGGGCGCAGCCACAACGTCGCCGCCCAGGCCACCACCTTGGGCAAGCGATTC
GCCTCGGCCGCCCAGGAGACGCTGATCGCGTTGGCCCGGCTGCGCGAGTTGATCGACCGTTACCCGTTGCGCGGCATCAA
GGGCCCGATGGGCACGTCGCAGGACATGCTCGACCTGATCGGCGGCGACCCGGCCAAGCTCGCCGAACTCGAGCGGCGGG
TCGCCGAATTCCTGGGTTTCTCAATGGTTTTGACCAGCGTCGGGCAGGTGTACCCGCGGTCGCTGGACCACGACGTGGTC
TCCGCGCTGGTGCAGCTGGGCGCCGGCCCCTCGTCGATGGCGCACACCATCCGGCTGATGGCCGGGCACGAACTGGTCAC
CGAGGGATTCGCGCCGGGTCAGGTCGGGTCGTCGGCGATGCCGCACAAGATGAACACCCGCAGCTGCGAACGGGTCAACG
GCCTACAGGTGGTGCTGCGCGGCTACGCCTCGATGGCCGCCGAACTGGCCGGCGCGCAGTGGAACGAGGGCGACGTGTTC
TGCTCGGTGGTGCGCCGAGTTGCATTGCCGGACAGCTTCTTTGCCATCGACGGGCAGATCGAGACGTTTCTGACGGTGCT
CGACGAGTTCGGCGCCTACCCGGCGGTGATCGGTCGCGAGCTGGACCGCTACCTGCCGTTCTTGGCCACCACCAAGGTGT
TGATCGCCGCGGTGCGCGCCGGGATGGGCCGCGAGGCCGCGCATCACGTGATCCGCGAGCACGCGGTGGCCACGGCATTG
GCGATGCGGGAACACGGCGCCGAGCCCGACCTGTTGGAGCGGCTGGCCGCCGACGAGCGGCTGCCGCTGGACCGGGCGGC
CCTGGACGCCGCGCTGGCCGACAGGCGGGCGTTCACCGGCGCCGCCGCCGACCAGGTGGACGAGGTGGCCGCCCAGGTGG
ACGCCCTGGTGAGCCGCTACCCGGACGCGGCCAAGTACACCCCGGGGGCCATCCTGTGA

Upstream 100 bases:

>100_bases
TCCAGACTGCCGCCAAACCGCGACCGCGGACGCCGTTACCCGGTGAATCCGTTGCCCGCCGCGGTCTCGCGGTGCTCGAG
AAACCGTAAGCTCTATGTCT

Downstream 100 bases:

>100_bases
CTCTCGCCGGCACGCTTTCGCAGATCGACTTCACCGATCTGGACAACTTCGCCAACGGATTTCCGCACCACCTGTTCGCC
GTGCATCGGCGCGAGGCGCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSIPNVLAARYASAEMVAIWSPEAKVVAERRLWLAVLRAQQQLGVAVPAEAIADYERVLEDVDLASIADRERLLRHDVKA
RIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLELVFAHGVAVAARLAERAVAYRDLVMAGRSHNVAAQATTLGKRF
ASAAQETLIALARLRELIDRYPLRGIKGPMGTSQDMLDLIGGDPAKLAELERRVAEFLGFSMVLTSVGQVYPRSLDHDVV
SALVQLGAGPSSMAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLRGYASMAAELAGAQWNEGDVF
CSVVRRVALPDSFFAIDGQIETFLTVLDEFGAYPAVIGRELDRYLPFLATTKVLIAAVRAGMGREAAHHVIREHAVATAL
AMREHGAEPDLLERLAADERLPLDRAALDAALADRRAFTGAAADQVDEVAAQVDALVSRYPDAAKYTPGAIL

Sequences:

>Translated_472_residues
MSIPNVLAARYASAEMVAIWSPEAKVVAERRLWLAVLRAQQQLGVAVPAEAIADYERVLEDVDLASIADRERLLRHDVKA
RIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLELVFAHGVAVAARLAERAVAYRDLVMAGRSHNVAAQATTLGKRF
ASAAQETLIALARLRELIDRYPLRGIKGPMGTSQDMLDLIGGDPAKLAELERRVAEFLGFSMVLTSVGQVYPRSLDHDVV
SALVQLGAGPSSMAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLRGYASMAAELAGAQWNEGDVF
CSVVRRVALPDSFFAIDGQIETFLTVLDEFGAYPAVIGRELDRYLPFLATTKVLIAAVRAGMGREAAHHVIREHAVATAL
AMREHGAEPDLLERLAADERLPLDRAALDAALADRRAFTGAAADQVDEVAAQVDALVSRYPDAAKYTPGAIL
>Mature_471_residues
SIPNVLAARYASAEMVAIWSPEAKVVAERRLWLAVLRAQQQLGVAVPAEAIADYERVLEDVDLASIADRERLLRHDVKAR
IEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLELVFAHGVAVAARLAERAVAYRDLVMAGRSHNVAAQATTLGKRFA
SAAQETLIALARLRELIDRYPLRGIKGPMGTSQDMLDLIGGDPAKLAELERRVAEFLGFSMVLTSVGQVYPRSLDHDVVS
ALVQLGAGPSSMAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLRGYASMAAELAGAQWNEGDVFC
SVVRRVALPDSFFAIDGQIETFLTVLDEFGAYPAVIGRELDRYLPFLATTKVLIAAVRAGMGREAAHHVIREHAVATALA
MREHGAEPDLLERLAADERLPLDRAALDAALADRRAFTGAAADQVDEVAAQVDALVSRYPDAAKYTPGAIL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=442, Percent_Identity=34.1628959276018, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=34.3832020997375, Blast_Score=213, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17508577, Length=466, Percent_Identity=28.3261802575107, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI32564234, Length=343, Percent_Identity=31.4868804664723, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6323391, Length=451, Percent_Identity=35.920177383592, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24647570, Length=462, Percent_Identity=33.7662337662338, Blast_Score=237, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51168; Mature: 51037

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPNVLAARYASAEMVAIWSPEAKVVAERRLWLAVLRAQQQLGVAVPAEAIADYERVLE
CCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DVDLASIADRERLLRHDVKARIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLELVF
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AHGVAVAARLAERAVAYRDLVMAGRSHNVAAQATTLGKRFASAAQETLIALARLRELIDR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPLRGIKGPMGTSQDMLDLIGGDPAKLAELERRVAEFLGFSMVLTSVGQVYPRSLDHDVV
CCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
SALVQLGAGPSSMAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLR
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GYASMAAELAGAQWNEGDVFCSVVRRVALPDSFFAIDGQIETFLTVLDEFGAYPAVIGRE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHH
LDRYLPFLATTKVLIAAVRAGMGREAAHHVIREHAVATALAMREHGAEPDLLERLAADER
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
LPLDRAALDAALADRRAFTGAAADQVDEVAAQVDALVSRYPDAAKYTPGAIL
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SIPNVLAARYASAEMVAIWSPEAKVVAERRLWLAVLRAQQQLGVAVPAEAIADYERVLE
CCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DVDLASIADRERLLRHDVKARIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLELVF
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AHGVAVAARLAERAVAYRDLVMAGRSHNVAAQATTLGKRFASAAQETLIALARLRELIDR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPLRGIKGPMGTSQDMLDLIGGDPAKLAELERRVAEFLGFSMVLTSVGQVYPRSLDHDVV
CCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
SALVQLGAGPSSMAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLR
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GYASMAAELAGAQWNEGDVFCSVVRRVALPDSFFAIDGQIETFLTVLDEFGAYPAVIGRE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHH
LDRYLPFLATTKVLIAAVRAGMGREAAHHVIREHAVATALAMREHGAEPDLLERLAADER
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
LPLDRAALDAALADRRAFTGAAADQVDEVAAQVDALVSRYPDAAKYTPGAIL
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]