Click here to switch to the map view.

The map label for this gene is garR [C]

Identifier: 41406702

GI number: 41406702

Start: 629050

End: 629928

Strand: Direct

Name: garR [C]

Synonym: MAP0604

Alternate gene names: 41406702

Gene position: 629050-629928 (Clockwise)

Preceding gene: 41406701

Following gene: 41406703

Centisome position: 13.02

GC content: 69.28

Gene sequence:

>879_bases
ATGACGACGAACGCGGCGCACACGACCGAGCAGCTGAAGCTCGGCTACATCGGGCTGGGCAATATGGGCGCGCCGATGGC
CACGAGGATGACCGAATGGCCGGGCGGGGTAACGGTTTACGACATCCGGATCGAGGCGATGACGCCGCTGGCCGAGGCGG
GCGCCACCCTTGCCGACAGCGTGGCCGACGTCGCCGCCGCCGACCTGATCCACATCACCGTGCTCGACGACGCCCAGGTG
CGTGAGGTGGTCGGCGAATTGGCCGGGCACGCCAAGCCCGGCACGGTGATCGCGATCCACTCGACCATCAGCGACACCAC
CGCCGTCGAACTGGCCCGCGATCTCAAGGCGCGGGACATCCACATCGTCGACGCCCCGGTCAGCGGCGGCGCGGCCGCCG
CCGCCCGGGGTGAGCTCGCCACCATGGTGGGCGCCGGCCGCGAGGTCTACGAACGGATCAAGCCGGCGTTCAAACACTGG
GCGGCGGTCGTCATCCACGCCGGTGAACCGGGTGCGGGAACCCGAATGAAGTTGGCCCGCAACATGTTGACGTTCACCTC
GTATGCCGCAGCGTGCGAGGCGATGAAGTTGGCCGAGGCGGCCGGGCTGGACCTGCAGGCGCTGGGCCGGGTGGTGCGTC
ATACCGACGCGCTCACCGGCGGTCCGGGCGCGATCATGGTGCGCGACAACATGAAAGACCTCGAGCCGGACAACTTCCTC
TACCAGCCGTTCTTGCACACCCGCGGCCTGGGGGAGAAGGATCTGAGCCTGGCGCTGGCGTTGGGTGAGGCGGTCTCGGT
CGACCTGCCGCTGGCCCGGCTGGCGTACGAGGGACTGGCGGCCGGGCTCGGCGTGCCACACACGGAGAAAGAGGCGTGA

Upstream 100 bases:

>100_bases
CCGACGACCTGGTCGGCATGTGCCTGTTCCTGCTGTCCGACGAGGCGTCGTGGATCACCGGGCAGATCTTCAACGTCGAC
GGCGGGCAGATAATCCGATC

Downstream 100 bases:

>100_bases
TGGACGAACGGCGGCGCAAGGGCCTCGAGAAGATGAACGAGGTCTACGGCTGGGAGATGCCCAACGTCGAGGGCGACGCG
TATTTCGACCTGACCGTCGA

Product: hypothetical protein

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTTNAAHTTEQLKLGYIGLGNMGAPMATRMTEWPGGVTVYDIRIEAMTPLAEAGATLADSVADVAAADLIHITVLDDAQV
REVVGELAGHAKPGTVIAIHSTISDTTAVELARDLKARDIHIVDAPVSGGAAAAARGELATMVGAGREVYERIKPAFKHW
AAVVIHAGEPGAGTRMKLARNMLTFTSYAAACEAMKLAEAAGLDLQALGRVVRHTDALTGGPGAIMVRDNMKDLEPDNFL
YQPFLHTRGLGEKDLSLALALGEAVSVDLPLARLAYEGLAAGLGVPHTEKEA

Sequences:

>Translated_292_residues
MTTNAAHTTEQLKLGYIGLGNMGAPMATRMTEWPGGVTVYDIRIEAMTPLAEAGATLADSVADVAAADLIHITVLDDAQV
REVVGELAGHAKPGTVIAIHSTISDTTAVELARDLKARDIHIVDAPVSGGAAAAARGELATMVGAGREVYERIKPAFKHW
AAVVIHAGEPGAGTRMKLARNMLTFTSYAAACEAMKLAEAAGLDLQALGRVVRHTDALTGGPGAIMVRDNMKDLEPDNFL
YQPFLHTRGLGEKDLSLALALGEAVSVDLPLARLAYEGLAAGLGVPHTEKEA
>Mature_291_residues
TTNAAHTTEQLKLGYIGLGNMGAPMATRMTEWPGGVTVYDIRIEAMTPLAEAGATLADSVADVAAADLIHITVLDDAQVR
EVVGELAGHAKPGTVIAIHSTISDTTAVELARDLKARDIHIVDAPVSGGAAAAARGELATMVGAGREVYERIKPAFKHWA
AVVIHAGEPGAGTRMKLARNMLTFTSYAAACEAMKLAEAAGLDLQALGRVVRHTDALTGGPGAIMVRDNMKDLEPDNFLY
QPFLHTRGLGEKDLSLALALGEAVSVDLPLARLAYEGLAAGLGVPHTEKEA

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=279, Percent_Identity=27.9569892473118, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI23308751, Length=208, Percent_Identity=31.7307692307692, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=33.8028169014084, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1786719, Length=269, Percent_Identity=31.2267657992565, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789092, Length=277, Percent_Identity=33.5740072202166, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790315, Length=269, Percent_Identity=31.5985130111524, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=31.6363636363636, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24655230, Length=280, Percent_Identity=30.3571428571429, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19922568, Length=280, Percent_Identity=30.3571428571429, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28574115, Length=269, Percent_Identity=24.907063197026, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30503; Mature: 30372

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNAAHTTEQLKLGYIGLGNMGAPMATRMTEWPGGVTVYDIRIEAMTPLAEAGATLADS
CCCCCCCCHHHEEEEEEECCCCCCCHHHHHHCCCCCEEEEEEEEEEECCHHHHCCHHHHH
VADVAAADLIHITVLDDAQVREVVGELAGHAKPGTVIAIHSTISDTTAVELARDLKARDI
HHHHHHHCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEE
HIVDAPVSGGAAAAARGELATMVGAGREVYERIKPAFKHWAAVVIHAGEPGAGTRMKLAR
EEEECCCCCCCHHHHCCHHHHHHHCCHHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHH
NMLTFTSYAAACEAMKLAEAAGLDLQALGRVVRHTDALTGGPGAIMVRDNMKDLEPDNFL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEE
YQPFLHTRGLGEKDLSLALALGEAVSVDLPLARLAYEGLAAGLGVPHTEKEA
ECCHHHHCCCCCHHHEEEEECCCCEECCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TTNAAHTTEQLKLGYIGLGNMGAPMATRMTEWPGGVTVYDIRIEAMTPLAEAGATLADS
CCCCCCCHHHEEEEEEECCCCCCCHHHHHHCCCCCEEEEEEEEEEECCHHHHCCHHHHH
VADVAAADLIHITVLDDAQVREVVGELAGHAKPGTVIAIHSTISDTTAVELARDLKARDI
HHHHHHHCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEE
HIVDAPVSGGAAAAARGELATMVGAGREVYERIKPAFKHWAAVVIHAGEPGAGTRMKLAR
EEEECCCCCCCHHHHCCHHHHHHHCCHHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHH
NMLTFTSYAAACEAMKLAEAAGLDLQALGRVVRHTDALTGGPGAIMVRDNMKDLEPDNFL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEE
YQPFLHTRGLGEKDLSLALALGEAVSVDLPLARLAYEGLAAGLGVPHTEKEA
ECCHHHHCCCCCHHHEEEEECCCCEECCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]