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The map label for this gene is ispF

Identifier: 41406575

GI number: 41406575

Start: 503778

End: 504257

Strand: Direct

Name: ispF

Synonym: MAP0477

Alternate gene names: 41406575

Gene position: 503778-504257 (Clockwise)

Preceding gene: 41406574

Following gene: 41406576

Centisome position: 10.43

GC content: 76.67

Gene sequence:

>480_bases
GTGAACACCACCCCGCGGATCGGCCTGGGCGTCGACGTGCACCCGATCCAGCCCGGGCGGCCGTGCCGGCTGCTGGGCCT
GCTTTTCGACGACGCCGACGGCTGCGCCGGCCACTCCGACGGCGACGTGGGCGCGCACGCGCTGTGCGACGCCGTGTTGT
CGGCGGCGGGCCTGGGCGACGTGGGTGCGGTGTTCGGCGTCGACGATCCGCGCTGGGCGGGTGTCAGCGGCGCCGACATG
CTGCGCCACGTCGCCGATCTGACGGCGCGCCACGGCTTTCGGGTGGGCAACGCCGCCGTCCAGGTGATCGGGAACCGGCC
CAAGGTCGGGCCGCGCCGCGCCGAGGCGCAGCGGGTGCTGTCGGAGCTGCTGGGGGCGCCGGTGTCGGTGGCGGCGACGA
CGACCGACGGGCTCGGGCTGACCGGGCGCGGTGAGGGCCTGGCCGCCATCGCCACCGCGCTGGTGGTCCCCACCGGCTGA

Upstream 100 bases:

>100_bases
AGCACGTCGGCGGCCAGGTCCAGGTGGTCGACGGCGACCCGCTGGCCTTCAAGATCACCACCCAGCTCGATCTGCTGCTG
GCCGAGACCATCGTGCGCCG

Downstream 100 bases:

>100_bases
GCCCGCGCGGCGCGGACGCCGCGGAACCACCCGGTAAGCTGGCACGTCGTGACCGATCGCCCCCGCTTGCGGCTACACGA
CACGGCAGCCGGTGCTGTGC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNTTPRIGLGVDVHPIQPGRPCRLLGLLFDDADGCAGHSDGDVGAHALCDAVLSAAGLGDVGAVFGVDDPRWAGVSGADM
LRHVADLTARHGFRVGNAAVQVIGNRPKVGPRRAEAQRVLSELLGAPVSVAATTTDGLGLTGRGEGLAAIATALVVPTG

Sequences:

>Translated_159_residues
MNTTPRIGLGVDVHPIQPGRPCRLLGLLFDDADGCAGHSDGDVGAHALCDAVLSAAGLGDVGAVFGVDDPRWAGVSGADM
LRHVADLTARHGFRVGNAAVQVIGNRPKVGPRRAEAQRVLSELLGAPVSVAATTTDGLGLTGRGEGLAAIATALVVPTG
>Mature_159_residues
MNTTPRIGLGVDVHPIQPGRPCRLLGLLFDDADGCAGHSDGDVGAHALCDAVLSAAGLGDVGAVFGVDDPRWAGVSGADM
LRHVADLTARHGFRVGNAAVQVIGNRPKVGPRRAEAQRVLSELLGAPVSVAATTTDGLGLTGRGEGLAAIATALVVPTG

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=40.9090909090909, Blast_Score=100, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_MYCA1 (A0QAB4)

Other databases:

- EMBL:   CP000479
- RefSeq:   YP_879852.1
- ProteinModelPortal:   A0QAB4
- SMR:   A0QAB4
- STRING:   A0QAB4
- EnsemblBacteria:   EBMYCT00000013498
- GeneID:   4530535
- GenomeReviews:   CP000479_GR
- KEGG:   mav:MAV_0572
- TIGR:   MAV_0572
- eggNOG:   COG0245
- GeneTree:   EBGT00050000016933
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   MAVI243243:MAV_0572-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 15957; Mature: 15957

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTPRIGLGVDVHPIQPGRPCRLLGLLFDDADGCAGHSDGDVGAHALCDAVLSAAGLGD
CCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
VGAVFGVDDPRWAGVSGADMLRHVADLTARHGFRVGNAAVQVIGNRPKVGPRRAEAQRVL
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCHHHHHHHH
SELLGAPVSVAATTTDGLGLTGRGEGLAAIATALVVPTG
HHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNTTPRIGLGVDVHPIQPGRPCRLLGLLFDDADGCAGHSDGDVGAHALCDAVLSAAGLGD
CCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
VGAVFGVDDPRWAGVSGADMLRHVADLTARHGFRVGNAAVQVIGNRPKVGPRRAEAQRVL
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCHHHHHHHH
SELLGAPVSVAATTTDGLGLTGRGEGLAAIATALVVPTG
HHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA