Click here to switch to the map view.

The map label for this gene is mesJ

Identifier: 41406536

GI number: 41406536

Start: 466040

End: 467002

Strand: Direct

Name: mesJ

Synonym: MAP0438

Alternate gene names: 41406536

Gene position: 466040-467002 (Clockwise)

Preceding gene: 41406535

Following gene: 41406537

Centisome position: 9.65

GC content: 75.91

Gene sequence:

>963_bases
ATGGATCGACCGGGTGCTCTAGACCGGCTGCGCACCGCGGTCGAGGCGTTCAGCACGGCCCACCTGGGTGCGGTCGAACG
CTGGTGCGTGGCGCTGTCCGGCGGCCCGGATTCGCTGGCGCTCACCGCCGTGGCGGCCCGGCTGCGGCCCACCACCGCGG
TGATCGTCGACCACGGTCTGCAACCGGACTCGGCGGCCGTCGCGCAAACCGCCCGGGCCCAGGCAATTGCGTTGGGTTGT
GTTGCGGCGCAAGTCATTTCGGTTCACGTCGACGGCGAGGGCGGCCTGGAGGCGGCGGCGCGGCGGGCCCGCTACGCGGC
GCTGGCCGCGCACCGCGACGGGCCGGTGCTGTTGGGTCACACCCTCGACGATCAGGCCGAGACGGTGCTGTTGGGGCTCG
GCCGCGGGTCGGGGGTGCGCTCGATCGCGGGCATGCGTCCCCACGATCCGCCGTGGTGCCGGCCGCTGCTCGGGCAGCGG
CGCGCGGTGACCCACGCGGCATGCGCGGAGTTGGGCCTGACGGCGTGGCAGGACCCGCACAACAGCGACCGTCGGTTCGC
CCGGGCGCGCCTGCGCGCCGAGGTGCTGCCGCTGCTGGAAGAGGTGCTCGGCGGCGGCGTGGTCGAGGCGCTGGCCCGCA
CCGCGACGGCGCTGCGCGAGGACAACGAGTTGCTCGACGCGCTGGCCGAGCGGGCGCTGCCCGCCGCCCGGGCCGGGGCG
GGGCTGCAGGTGGCGGCGCTGGCCGGGCTGGACGCGCCCGTGCGGCGCCGGGTGATCCGGGCCTGGCTGCTGGCCGGCGG
TGCAACGGGATTGACCGACAAGCAGATTCGCGGGGTGGACAACCTGGTCACCGCCTGGCGCGGTCAGGGCGCGGTGGCGG
TGGGGTCGTCGCTGCCCGGCGAGCGGCTGTTCGCGGGGCGCCGCGACGGCGTGTTGACGCTGTGGCGCGAGCCGGTCCGT
TAA

Upstream 100 bases:

>100_bases
TCGACCAGGTGGTGGGCCGGGTCGGTATGGCGCGGTTCAACGCCATTTGGACGGGGCCTGAAACGCTGCCGCTGCCGGTC
GAAATCGAAGAGCCCCAGCG

Downstream 100 bases:

>100_bases
CCGCAATGCCGGAGCCGCCGTTGGTTATTCGCCCGCCGGCGTGGCACGCTGTGGGCGTGGCCCAGATCTCCTCGGCGATC
ACCCCCGCTCAGCCCGCGGA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MDRPGALDRLRTAVEAFSTAHLGAVERWCVALSGGPDSLALTAVAARLRPTTAVIVDHGLQPDSAAVAQTARAQAIALGC
VAAQVISVHVDGEGGLEAAARRARYAALAAHRDGPVLLGHTLDDQAETVLLGLGRGSGVRSIAGMRPHDPPWCRPLLGQR
RAVTHAACAELGLTAWQDPHNSDRRFARARLRAEVLPLLEEVLGGGVVEALARTATALREDNELLDALAERALPAARAGA
GLQVAALAGLDAPVRRRVIRAWLLAGGATGLTDKQIRGVDNLVTAWRGQGAVAVGSSLPGERLFAGRRDGVLTLWREPVR

Sequences:

>Translated_320_residues
MDRPGALDRLRTAVEAFSTAHLGAVERWCVALSGGPDSLALTAVAARLRPTTAVIVDHGLQPDSAAVAQTARAQAIALGC
VAAQVISVHVDGEGGLEAAARRARYAALAAHRDGPVLLGHTLDDQAETVLLGLGRGSGVRSIAGMRPHDPPWCRPLLGQR
RAVTHAACAELGLTAWQDPHNSDRRFARARLRAEVLPLLEEVLGGGVVEALARTATALREDNELLDALAERALPAARAGA
GLQVAALAGLDAPVRRRVIRAWLLAGGATGLTDKQIRGVDNLVTAWRGQGAVAVGSSLPGERLFAGRRDGVLTLWREPVR
>Mature_320_residues
MDRPGALDRLRTAVEAFSTAHLGAVERWCVALSGGPDSLALTAVAARLRPTTAVIVDHGLQPDSAAVAQTARAQAIALGC
VAAQVISVHVDGEGGLEAAARRARYAALAAHRDGPVLLGHTLDDQAETVLLGLGRGSGVRSIAGMRPHDPPWCRPLLGQR
RAVTHAACAELGLTAWQDPHNSDRRFARARLRAEVLPLLEEVLGGGVVEALARTATALREDNELLDALAERALPAARAGA
GLQVAALAGLDAPVRRRVIRAWLLAGGATGLTDKQIRGVDNLVTAWRGQGAVAVGSSLPGERLFAGRRDGVLTLWREPVR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=311, Percent_Identity=32.7974276527331, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_MYCPA (Q744A3)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_959372.1
- ProteinModelPortal:   Q744A3
- SMR:   Q744A3
- EnsemblBacteria:   EBMYCT00000037874
- GeneID:   2720208
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP0438
- NMPDR:   fig|262316.1.peg.438
- GeneTree:   EBGT00050000016642
- HOGENOM:   HBG692699
- OMA:   QDPHNSD
- ProtClustDB:   CLSK792603
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946

EC number: NA

Molecular weight: Translated: 33522; Mature: 33522

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRPGALDRLRTAVEAFSTAHLGAVERWCVALSGGPDSLALTAVAARLRPTTAVIVDHGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCEEEEEECCC
QPDSAAVAQTARAQAIALGCVAAQVISVHVDGEGGLEAAARRARYAALAAHRDGPVLLGH
CCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEC
TLDDQAETVLLGLGRGSGVRSIAGMRPHDPPWCRPLLGQRRAVTHAACAELGLTAWQDPH
CCCCCCCEEEEECCCCCCHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCHHCCCCC
NSDRRFARARLRAEVLPLLEEVLGGGVVEALARTATALREDNELLDALAERALPAARAGA
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCC
GLQVAALAGLDAPVRRRVIRAWLLAGGATGLTDKQIRGVDNLVTAWRGQGAVAVGSSLPG
CEEEEHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHCCCCCEEECCCCCC
ERLFAGRRDGVLTLWREPVR
HHHHCCCCCCEEEECCCCCC
>Mature Secondary Structure
MDRPGALDRLRTAVEAFSTAHLGAVERWCVALSGGPDSLALTAVAARLRPTTAVIVDHGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCEEEEEECCC
QPDSAAVAQTARAQAIALGCVAAQVISVHVDGEGGLEAAARRARYAALAAHRDGPVLLGH
CCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEC
TLDDQAETVLLGLGRGSGVRSIAGMRPHDPPWCRPLLGQRRAVTHAACAELGLTAWQDPH
CCCCCCCEEEEECCCCCCHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCHHCCCCC
NSDRRFARARLRAEVLPLLEEVLGGGVVEALARTATALREDNELLDALAERALPAARAGA
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCC
GLQVAALAGLDAPVRRRVIRAWLLAGGATGLTDKQIRGVDNLVTAWRGQGAVAVGSSLPG
CEEEEHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHCCCCCEEECCCCCC
ERLFAGRRDGVLTLWREPVR
HHHHCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA