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The map label for this gene is holB [H]

Identifier: 41406525

GI number: 41406525

Start: 453987

End: 455192

Strand: Direct

Name: holB [H]

Synonym: MAP0427

Alternate gene names: 41406525

Gene position: 453987-455192 (Clockwise)

Preceding gene: 41406523

Following gene: 41406526

Centisome position: 9.4

GC content: 75.54

Gene sequence:

>1206_bases
ATGTCCGGGGTGTTTACGCGGCTGGTAGGCCAGGACGCGGTGAAGGCCGAGCTGCTCGGTGCGGCCCGGGCCGCCCGCGG
TGATGCGGGCCACAGCGACGCGGGCGCCGGGACTATGACACACGCCTGGCTGATCACGGGCCCGCCGGGGTCCGGACGCT
CGGTGGCGGCGCTGTGCTTCGCGGCCGCGCTGCAATGCACCGCGGACGGCGAACCGGGTTGTGGCCGCTGCCATGCCTGC
ACGACGACCCTGGCCGGCACCCACGCCGACGTGCGCCGGGTGGTTCCCGAGGGCCTGTCGATCGGCGTCGACGAGATGCG
CGCCATCGTGCAGATCGCCGCGCGGCGCCCGGCCACCGGGAACTGGCAGATCGTGGTGATCGAGGACGCCGACCGGTTGA
CCGAGGGGGCTGCCAACGCGCTGCTGAAGGTGGTCGAGGAGCCGCCGCCGTCGACGGTGTTTCTGCTGTGCGCGCCGTCG
GTGGACCCCGAGGACGTGGCGATTACGCTGCGGTCCCGGTGCCGGCACGTCGCGCTGGTGACGCCGTCGACCGAGGCGAT
CGCCCGGGTGCTGGTCGACAGCGACGGCCTCACCCCCGAGACGGCGGACTGGGCGGCGTCGGTCAGCGGCGGCCATGTGG
GCCGGGCGCGGCGGCTGGCCACCGACCCCGAGGCGCGGCAGCGCCGGGAGCGGGCGCTGGGCCTGGTGCGCGACGCGGCC
ACGCCGTCGCGGGCCTACGCCGCGGCCGAAGAGTTGGTGGCCGCGGCCGAGGCGGAGGCGGTGGTGCTGACCGCCGAGCG
CGCCGAGGCCGAGACCGAGGAGCTGCGCACGGCGCTGGGCGCCGGCGGCACCGGCAAGGGCACCGCGGGCGCCATGCGCG
GGGCGGCCGGGGCGATCAAAGACCTTGAGCGGTGCCAGAAGTCGCGTCAGACCCGGGCGTCGCGGGATGCGCTGGACCGG
GCGCTGATCGACCTGGCGACCTACTTCCGGGACGCGCTGATGGCCTCCTCCGGCGCCGGGGCGGTGCGGGCCAACCACCC
GGACATGGCCGAGCGGGTCGCGGCGCTGGCCGCGCACGCGCCGCCCGAGCGGCTGCTGCGCTGCATCGAGGCGGTGCTGG
AGTGCCGCGAGGCGCTGGCGGTCAACGTCAAGCCCAAGTTCGCCGTCGACGCCATGGTCGCCACCATCGGCCAGCAACTT
GGGTGA

Upstream 100 bases:

>100_bases
CAGGTCATTTCGGCGGCGCGACGGCGGCGAAGATAACGGAATGGTCGCGGTATGGTGGCCGATTGGTATCTGTCGGGCGG
TCGCAGTAGGTTAGGCGCTG

Downstream 100 bases:

>100_bases
CGCGACCCCTCTGCCGTAGACTCGTGCCGCCCATCGGGCACGCCACCTTAGCTCAGTCGGTAGAGCGGCTCACTCGTAAT
GAGCAGGTCAGGAGTTCGAT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSGVFTRLVGQDAVKAELLGAARAARGDAGHSDAGAGTMTHAWLITGPPGSGRSVAALCFAAALQCTADGEPGCGRCHAC
TTTLAGTHADVRRVVPEGLSIGVDEMRAIVQIAARRPATGNWQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPS
VDPEDVAITLRSRCRHVALVTPSTEAIARVLVDSDGLTPETADWAASVSGGHVGRARRLATDPEARQRRERALGLVRDAA
TPSRAYAAAEELVAAAEAEAVVLTAERAEAETEELRTALGAGGTGKGTAGAMRGAAGAIKDLERCQKSRQTRASRDALDR
ALIDLATYFRDALMASSGAGAVRANHPDMAERVAALAAHAPPERLLRCIEAVLECREALAVNVKPKFAVDAMVATIGQQL
G

Sequences:

>Translated_401_residues
MSGVFTRLVGQDAVKAELLGAARAARGDAGHSDAGAGTMTHAWLITGPPGSGRSVAALCFAAALQCTADGEPGCGRCHAC
TTTLAGTHADVRRVVPEGLSIGVDEMRAIVQIAARRPATGNWQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPS
VDPEDVAITLRSRCRHVALVTPSTEAIARVLVDSDGLTPETADWAASVSGGHVGRARRLATDPEARQRRERALGLVRDAA
TPSRAYAAAEELVAAAEAEAVVLTAERAEAETEELRTALGAGGTGKGTAGAMRGAAGAIKDLERCQKSRQTRASRDALDR
ALIDLATYFRDALMASSGAGAVRANHPDMAERVAALAAHAPPERLLRCIEAVLECREALAVNVKPKFAVDAMVATIGQQL
G
>Mature_400_residues
SGVFTRLVGQDAVKAELLGAARAARGDAGHSDAGAGTMTHAWLITGPPGSGRSVAALCFAAALQCTADGEPGCGRCHACT
TTLAGTHADVRRVVPEGLSIGVDEMRAIVQIAARRPATGNWQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPSV
DPEDVAITLRSRCRHVALVTPSTEAIARVLVDSDGLTPETADWAASVSGGHVGRARRLATDPEARQRRERALGLVRDAAT
PSRAYAAAEELVAAAEAEAVVLTAERAEAETEELRTALGAGGTGKGTAGAMRGAAGAIKDLERCQKSRQTRASRDALDRA
LIDLATYFRDALMASSGAGAVRANHPDMAERVAALAAHAPPERLLRCIEAVLECREALAVNVKPKFAVDAMVATIGQQLG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=152, Percent_Identity=30.2631578947368, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787341, Length=153, Percent_Identity=37.9084967320261, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41578; Mature: 41446

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGVFTRLVGQDAVKAELLGAARAARGDAGHSDAGAGTMTHAWLITGPPGSGRSVAALCF
CCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHH
AAALQCTADGEPGCGRCHACTTTLAGTHADVRRVVPEGLSIGVDEMRAIVQIAARRPATG
HHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
NWQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPSVDPEDVAITLRSRCRHVALV
CEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHEEHHCCCEEEEE
TPSTEAIARVLVDSDGLTPETADWAASVSGGHVGRARRLATDPEARQRRERALGLVRDAA
CCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCC
TPSRAYAAAEELVAAAEAEAVVLTAERAEAETEELRTALGAGGTGKGTAGAMRGAAGAIK
CCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DLERCQKSRQTRASRDALDRALIDLATYFRDALMASSGAGAVRANHPDMAERVAALAAHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCC
PPERLLRCIEAVLECREALAVNVKPKFAVDAMVATIGQQLG
CHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGVFTRLVGQDAVKAELLGAARAARGDAGHSDAGAGTMTHAWLITGPPGSGRSVAALCF
CCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHH
AAALQCTADGEPGCGRCHACTTTLAGTHADVRRVVPEGLSIGVDEMRAIVQIAARRPATG
HHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
NWQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPSVDPEDVAITLRSRCRHVALV
CEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHEEHHCCCEEEEE
TPSTEAIARVLVDSDGLTPETADWAASVSGGHVGRARRLATDPEARQRRERALGLVRDAA
CCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCC
TPSRAYAAAEELVAAAEAEAVVLTAERAEAETEELRTALGAGGTGKGTAGAMRGAAGAIK
CCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DLERCQKSRQTRASRDALDRALIDLATYFRDALMASSGAGAVRANHPDMAERVAALAAHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCC
PPERLLRCIEAVLECREALAVNVKPKFAVDAMVATIGQQLG
CHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]