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The map label for this gene is walK [H]

Identifier: 41406358

GI number: 41406358

Start: 279272

End: 280762

Strand: Direct

Name: walK [H]

Synonym: MAP0260

Alternate gene names: 41406358

Gene position: 279272-280762 (Clockwise)

Preceding gene: 41406357

Following gene: 41406360

Centisome position: 5.78

GC content: 71.76

Gene sequence:

>1491_bases
TTGGCTGACACGCGCCGGGTCTGGTCGCTGCGGCTGCGGCTGCTGGTCGGACAGATCGTCGTGCTGGCGCTGGTGTGTCT
GGGCATCACCGCGGTCACCGAACTGGCGCTGAACCATCACCTGGTCAAGCAGCTCGACGGCCAACTCGCCGGAACCTCGT
TCCGTTCGGCGCTGATGTACCCCGAGCCCAACCATCCGCGCCACGAGCACTCGCCGTATCCGAGGCCGGGCCCCGGCCCG
CGGTTCCTGGACGCCCCGGGGCAGCCGGCGGGCATGGTCGCCGCGGTGGTCAGCCACGGCAAGACCGTCGACGCCGGCTA
CCTCACCAGCAGCGGCGCCCGGGACGCGCTGACGTCGACCGCCCAGACCCAACTCGAACAGATCGCCGGCAGCCGCGCAC
CGGTCACCGTGAACCTGGACGGTCTGGGTCGCTACCGCGTCGTCGCCGCCCCCAGCCGCAACGGCAGCGACATCATCGTC
ACCGGCCTGTCGATGGCCAACATCGACGCCACCCTGATCCGGATGCTGGTCATCTTCGGGATCGTCACCGTGATCGCGCT
GGCGGCCGCGACCATCGCCGGGGTGGTGATCATCAGGCGGGCACTGGCGCCGTTGCGCCGGGTGGCACAAACCGCGCGCG
AGGTGGCCGACCTGCCGCTGGCGCGCGGCGAGGTCGAATTGCCGGTGCGGGTACGGGAATCGGACGCCAACCCGTCCACC
GAGGTGGGCCAGCTCGGCGCCGCGCTCAACCGGATGCTCGACCACATCGCCGACGCGCTGTCGGCGCGCCAGGCCAGCGA
GACCCGGGTACGCCAGTTCGTCGCCGACGCCAGTCACGAACTGCGCACCCCGCTGGCCGCGATCCGCGGCTACACCGAGC
TGACCCAGCGCATGGGCGACGACCGCGAGGCGGTGGCGCAGGCGATGAGCCGGGTGGCCTCGGAGACCGAGCGGATGACC
CGGCTGGTCGAGGACCTGCTGCTGCTGGCGCGGCTGGATTCCGGCCGCCCGCTGGAGCGCGAACCGGTCGACCTGTCCCG
GCTCGCGGTGGACGCGGTCAACGACGCCCACGTCGCCGGCCCTGACCATCAGTGGGAACTCGACCTGCCCGAGGAACCGG
TGGTGGTCACCGGGGACGCGGCGCGGCTGCACCAGGTGCTGACCAACCTGCTCGCCAACGCGCGGGTGCACACCGGCGCG
GGCACCGTGGTGACCACCCGGCTGAGCACCGAGCCGGCGCACACCGTGCTGCAGGTCATCGACAACGGGCCGGGCATTCC
GGCGGAGCTGCAGTCGGAGGTGTTCGAACGGTTCGCCCGCGGCGACTCCTCGCGGTCGCGCAAGGGTGGCAGCACCGGGC
TGGGCCTGGCCATCGTCTCGGCGGTGGTCAAGGCGCACAACGGGACGATCTCGGTGAACAGCTCGCCGGGCCACACCGAG
TTCACGGTGCGGTTGCCACCCAACGGATGGCAACCGCACGCGTCGGCGTAG

Upstream 100 bases:

>100_bases
GAGCGATCGCAAGCGCGGCGAAGCCGGGCGCAGCGGGTCGCGACCATTGGCTAGGAGCGATCGCAAGCGCGGCGAAGCCG
GGCGCGGCGGGTCGCGACCA

Downstream 100 bases:

>100_bases
GTCAGCTGTTGCAGGTCACATCGATCTCGAACGGCTTGTTGACCGGCTGCAGCGGGTTGGCCATGTCGACCCCGGTCGCG
GTGCCGGTGATCTTGTAGCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MADTRRVWSLRLRLLVGQIVVLALVCLGITAVTELALNHHLVKQLDGQLAGTSFRSALMYPEPNHPRHEHSPYPRPGPGP
RFLDAPGQPAGMVAAVVSHGKTVDAGYLTSSGARDALTSTAQTQLEQIAGSRAPVTVNLDGLGRYRVVAAPSRNGSDIIV
TGLSMANIDATLIRMLVIFGIVTVIALAAATIAGVVIIRRALAPLRRVAQTAREVADLPLARGEVELPVRVRESDANPST
EVGQLGAALNRMLDHIADALSARQASETRVRQFVADASHELRTPLAAIRGYTELTQRMGDDREAVAQAMSRVASETERMT
RLVEDLLLLARLDSGRPLEREPVDLSRLAVDAVNDAHVAGPDHQWELDLPEEPVVVTGDAARLHQVLTNLLANARVHTGA
GTVVTTRLSTEPAHTVLQVIDNGPGIPAELQSEVFERFARGDSSRSRKGGSTGLGLAIVSAVVKAHNGTISVNSSPGHTE
FTVRLPPNGWQPHASA

Sequences:

>Translated_496_residues
MADTRRVWSLRLRLLVGQIVVLALVCLGITAVTELALNHHLVKQLDGQLAGTSFRSALMYPEPNHPRHEHSPYPRPGPGP
RFLDAPGQPAGMVAAVVSHGKTVDAGYLTSSGARDALTSTAQTQLEQIAGSRAPVTVNLDGLGRYRVVAAPSRNGSDIIV
TGLSMANIDATLIRMLVIFGIVTVIALAAATIAGVVIIRRALAPLRRVAQTAREVADLPLARGEVELPVRVRESDANPST
EVGQLGAALNRMLDHIADALSARQASETRVRQFVADASHELRTPLAAIRGYTELTQRMGDDREAVAQAMSRVASETERMT
RLVEDLLLLARLDSGRPLEREPVDLSRLAVDAVNDAHVAGPDHQWELDLPEEPVVVTGDAARLHQVLTNLLANARVHTGA
GTVVTTRLSTEPAHTVLQVIDNGPGIPAELQSEVFERFARGDSSRSRKGGSTGLGLAIVSAVVKAHNGTISVNSSPGHTE
FTVRLPPNGWQPHASA
>Mature_495_residues
ADTRRVWSLRLRLLVGQIVVLALVCLGITAVTELALNHHLVKQLDGQLAGTSFRSALMYPEPNHPRHEHSPYPRPGPGPR
FLDAPGQPAGMVAAVVSHGKTVDAGYLTSSGARDALTSTAQTQLEQIAGSRAPVTVNLDGLGRYRVVAAPSRNGSDIIVT
GLSMANIDATLIRMLVIFGIVTVIALAAATIAGVVIIRRALAPLRRVAQTAREVADLPLARGEVELPVRVRESDANPSTE
VGQLGAALNRMLDHIADALSARQASETRVRQFVADASHELRTPLAAIRGYTELTQRMGDDREAVAQAMSRVASETERMTR
LVEDLLLLARLDSGRPLEREPVDLSRLAVDAVNDAHVAGPDHQWELDLPEEPVVVTGDAARLHQVLTNLLANARVHTGAG
TVVTTRLSTEPAHTVLQVIDNGPGIPAELQSEVFERFARGDSSRSRKGGSTGLGLAIVSAVVKAHNGTISVNSSPGHTEF
TVRLPPNGWQPHASA

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=323, Percent_Identity=29.7213622291022, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1788393, Length=292, Percent_Identity=32.1917808219178, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1786600, Length=221, Percent_Identity=34.3891402714932, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790861, Length=346, Percent_Identity=29.1907514450867, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1789149, Length=359, Percent_Identity=26.7409470752089, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693157, Length=239, Percent_Identity=32.2175732217573, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790346, Length=286, Percent_Identity=27.972027972028, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=35.1931330472103, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788713, Length=224, Percent_Identity=30.8035714285714, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787894, Length=247, Percent_Identity=32.7935222672065, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789403, Length=353, Percent_Identity=28.8951841359773, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788279, Length=318, Percent_Identity=26.7295597484277, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788549, Length=248, Percent_Identity=29.8387096774194, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789808, Length=228, Percent_Identity=29.3859649122807, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790300, Length=240, Percent_Identity=30.4166666666667, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081816, Length=353, Percent_Identity=28.6118980169972, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790551, Length=318, Percent_Identity=28.9308176100629, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87082128, Length=224, Percent_Identity=29.9107142857143, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=32.1428571428571, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI48994928, Length=222, Percent_Identity=29.2792792792793, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52881; Mature: 52750

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTRRVWSLRLRLLVGQIVVLALVCLGITAVTELALNHHLVKQLDGQLAGTSFRSALMY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECC
PEPNHPRHEHSPYPRPGPGPRFLDAPGQPAGMVAAVVSHGKTVDAGYLTSSGARDALTST
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
AQTQLEQIAGSRAPVTVNLDGLGRYRVVAAPSRNGSDIIVTGLSMANIDATLIRMLVIFG
HHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
IVTVIALAAATIAGVVIIRRALAPLRRVAQTAREVADLPLARGEVELPVRVRESDANPST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEEEECCCCCCCH
EVGQLGAALNRMLDHIADALSARQASETRVRQFVADASHELRTPLAAIRGYTELTQRMGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DREAVAQAMSRVASETERMTRLVEDLLLLARLDSGRPLEREPVDLSRLAVDAVNDAHVAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
PDHQWELDLPEEPVVVTGDAARLHQVLTNLLANARVHTGAGTVVTTRLSTEPAHTVLQVI
CCCCEECCCCCCCEEEECCHHHHHHHHHHHHHCCEEECCCCCEEEEECCCCHHHHHHHHH
DNGPGIPAELQSEVFERFARGDSSRSRKGGSTGLGLAIVSAVVKAHNGTISVNSSPGHTE
HCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCEE
FTVRLPPNGWQPHASA
EEEEECCCCCCCCCCC
>Mature Secondary Structure 
ADTRRVWSLRLRLLVGQIVVLALVCLGITAVTELALNHHLVKQLDGQLAGTSFRSALMY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECC
PEPNHPRHEHSPYPRPGPGPRFLDAPGQPAGMVAAVVSHGKTVDAGYLTSSGARDALTST
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
AQTQLEQIAGSRAPVTVNLDGLGRYRVVAAPSRNGSDIIVTGLSMANIDATLIRMLVIFG
HHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
IVTVIALAAATIAGVVIIRRALAPLRRVAQTAREVADLPLARGEVELPVRVRESDANPST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEEEECCCCCCCH
EVGQLGAALNRMLDHIADALSARQASETRVRQFVADASHELRTPLAAIRGYTELTQRMGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DREAVAQAMSRVASETERMTRLVEDLLLLARLDSGRPLEREPVDLSRLAVDAVNDAHVAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
PDHQWELDLPEEPVVVTGDAARLHQVLTNLLANARVHTGAGTVVTTRLSTEPAHTVLQVI
CCCCEECCCCCCCEEEECCHHHHHHHHHHHHHCCEEECCCCCEEEEECCCCHHHHHHHHH
DNGPGIPAELQSEVFERFARGDSSRSRKGGSTGLGLAIVSAVVKAHNGTISVNSSPGHTE
HCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCEE
FTVRLPPNGWQPHASA
EEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA