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The map label for this gene is gyrB

Identifier: 41406103

GI number: 41406103

Start: 5258

End: 7291

Strand: Direct

Name: gyrB

Synonym: MAP0005

Alternate gene names: 41406103

Gene position: 5258-7291 (Clockwise)

Preceding gene: 41406102

Following gene: 41406104

Centisome position: 0.11

GC content: 64.01

Gene sequence:

>2034_bases
GTGGCTGCCCAGAAGAAGAAGGCGCAAGACGAATACGGTGCTTCAGCGATCACCGTCCTGGAAGGACTGGAGGCGGTCCG
CAAACGCCCCGGCATGTACATCGGCTCTACCGGCGAGCGAGGTCTGCACCACCTCATCTGGGAGGTGGTCGACAACTCGG
TCGACGAAGCGATGGCCGGCTACGCCGACCGGGTCGACGTGCGGATCCTGGACGACGGCAGCGTTGAGGTCGCCGACAAC
GGCCGCGGCATCCCCGTCGCGATGCACGCGACCGGCGCCCCCACCGTCGACGTGGTGATGACGCAGCTGCACGCCGGCGG
CAAGTTCGGCGGCGAAAACAGCGGCTACAACGTCAGCGGCGGTCTGCACGGCGTCGGCGTCTCGGTGGTCAACGCACTGT
CCACCCGGCTCGAGGTCAACATCGCCCGCGACGGCTACGAGTGGTCGCAGTACTACGACCACGCCGTGCCCGGCACCCTC
AAACAGGGCGAGGCCACCAAGCGCACCGGCACCACCATCCGGTTCTGGGCCGACCCCGACATCTTCGAGACCACCGAGTA
CGACTTCGAAACGGTGGCCCGACGGCTGCAGGAAATGGCGTTCCTCAACAAGGGCCTGACCATCAACCTCACCGACGAGC
GGGTGACCAACGAAGAGGTCGTCGACGAGGTGGTCAGCGACACCGCCGACGCACCCAAGTCGGCGCAGGAGAAGGCGGCG
GAATCGGCTGCGCCGCATAAGGTCAAGCACCGCACCTTCCACTACCCCGGCGGCCTGGTCGACTTCGTCAAACACATCAA
TCGCACCAAAAACCCCATCCACCAGAGCATCATCGATTTCGGTGGGAAGGGCCCCGGCCACGAGGTCGAGATCGCGATGC
AGTGGAACGGCGGCTATTCCGAATCGGTGCACACGTTCGCCAACACCATCAACACGCACGAGGGCGGCACCCACGAGGAG
GGCTTCCGCAGCGCGTTGACCTCCGTGGTCAACAAGTACGCCAAGGACAAGAAGCTGCTCAAGGACAAGGACCCCAACCT
CACCGGCGACGACATCCGCGAGGGTTTGGCCGCGGTGATCTCGGTCAAGGTGAGCGAACCGCAGTTCGAGGGCCAGACCA
AGACCAAACTGGGCAACACCGAGGTGAAGTCGTTCGTGCAGAAGGTGTGCAACGAACAGCTCACCCACTGGTTCGAAGCC
AACCCCGCAGACGCCAAAGTCATTGTCAACAAGGCGGTTTCGTCAGCGCAGGCGCGCATTGCCGCGCGCAAGGCGCGAGA
GTTGGTGCGCCGCAAGAGCGCAACCGACCTGGGCGGGCTGCCCGGCAAGCTCGCCGACTGCCGGTCGACCGATCCGCGCA
AGTCGGAATTGTATGTGGTCGAGGGTGACTCGGCCGGCGGCTCGGCGAAAAGCGGCCGGGACTCGATGTTCCAGGCCATC
CTTCCGCTGCGCGGCAAGATCATCAACGTCGAAAAGGCCCGCATCGACCGGGTTTTGAAGAACACCGAAGTGCAGGCGAT
CATCACCGCGCTGGGCACCGGGATTCACGACGAGTTCGACATCACCAAGCTGCGCTACCACAAGATCGTGTTGATGGCCG
ACGCCGACGTGGACGGCCAGCACATCTCGACGCTGTTGTTGACGCTGCTGTTCCGGTTCATGCGGCCGCTGATCGAACAC
GGGCACGTGTTCTTGGCCCAGCCACCGCTGTACAAGCTGAAATGGCAGCGCAGCGATCCAGAGTTCGCCTACTCCGACCG
CGAGCGGGACGGGCTGCTCGAGGCCGGCCTGAAGGCCGGCAAGAAGATCAACAAGGACGACGGTATCCAGCGCTACAAGG
GTCTGGGCGAGATGGACGCCAAGGAATTGTGGGAAACCACAATGGATCCCACCGTGCGGGTGCTGCGCCAGGTCACGCTG
GACGACGCCGCGGCCGCCGACGAGCTGTTCTCCATCCTGATGGGCGAGGACGTCGACGCGCGCCGCAGCTTCATCACCCG
CAATGCCAAAGACGTTCGCTTCCTAGACGTTTAA

Upstream 100 bases:

>100_bases
AAAATCGGTGCGGACCGCTCGTCACGGTAGACTGGCAGGATGCGATTGTTGCGGTGATCAGGAACCGCCCGCATGAAACC
CCAAGGAGAGCTTTCCGACC

Downstream 100 bases:

>100_bases
ACGAGGAATAGATGACTGACACCACGCTGCCACCCGGCGGTGACGCCGCCGACCGCGTCGAACCGGTCGACATCCAGCAG
GAGATGCAGCGCAGCTACAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 677; Mature: 676

Protein sequence:

>677_residues
MAAQKKKAQDEYGASAITVLEGLEAVRKRPGMYIGSTGERGLHHLIWEVVDNSVDEAMAGYADRVDVRILDDGSVEVADN
GRGIPVAMHATGAPTVDVVMTQLHAGGKFGGENSGYNVSGGLHGVGVSVVNALSTRLEVNIARDGYEWSQYYDHAVPGTL
KQGEATKRTGTTIRFWADPDIFETTEYDFETVARRLQEMAFLNKGLTINLTDERVTNEEVVDEVVSDTADAPKSAQEKAA
ESAAPHKVKHRTFHYPGGLVDFVKHINRTKNPIHQSIIDFGGKGPGHEVEIAMQWNGGYSESVHTFANTINTHEGGTHEE
GFRSALTSVVNKYAKDKKLLKDKDPNLTGDDIREGLAAVISVKVSEPQFEGQTKTKLGNTEVKSFVQKVCNEQLTHWFEA
NPADAKVIVNKAVSSAQARIAARKARELVRRKSATDLGGLPGKLADCRSTDPRKSELYVVEGDSAGGSAKSGRDSMFQAI
LPLRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDITKLRYHKIVLMADADVDGQHISTLLLTLLFRFMRPLIEH
GHVFLAQPPLYKLKWQRSDPEFAYSDRERDGLLEAGLKAGKKINKDDGIQRYKGLGEMDAKELWETTMDPTVRVLRQVTL
DDAAAADELFSILMGEDVDARRSFITRNAKDVRFLDV

Sequences:

>Translated_677_residues
MAAQKKKAQDEYGASAITVLEGLEAVRKRPGMYIGSTGERGLHHLIWEVVDNSVDEAMAGYADRVDVRILDDGSVEVADN
GRGIPVAMHATGAPTVDVVMTQLHAGGKFGGENSGYNVSGGLHGVGVSVVNALSTRLEVNIARDGYEWSQYYDHAVPGTL
KQGEATKRTGTTIRFWADPDIFETTEYDFETVARRLQEMAFLNKGLTINLTDERVTNEEVVDEVVSDTADAPKSAQEKAA
ESAAPHKVKHRTFHYPGGLVDFVKHINRTKNPIHQSIIDFGGKGPGHEVEIAMQWNGGYSESVHTFANTINTHEGGTHEE
GFRSALTSVVNKYAKDKKLLKDKDPNLTGDDIREGLAAVISVKVSEPQFEGQTKTKLGNTEVKSFVQKVCNEQLTHWFEA
NPADAKVIVNKAVSSAQARIAARKARELVRRKSATDLGGLPGKLADCRSTDPRKSELYVVEGDSAGGSAKSGRDSMFQAI
LPLRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDITKLRYHKIVLMADADVDGQHISTLLLTLLFRFMRPLIEH
GHVFLAQPPLYKLKWQRSDPEFAYSDRERDGLLEAGLKAGKKINKDDGIQRYKGLGEMDAKELWETTMDPTVRVLRQVTL
DDAAAADELFSILMGEDVDARRSFITRNAKDVRFLDV
>Mature_676_residues
AAQKKKAQDEYGASAITVLEGLEAVRKRPGMYIGSTGERGLHHLIWEVVDNSVDEAMAGYADRVDVRILDDGSVEVADNG
RGIPVAMHATGAPTVDVVMTQLHAGGKFGGENSGYNVSGGLHGVGVSVVNALSTRLEVNIARDGYEWSQYYDHAVPGTLK
QGEATKRTGTTIRFWADPDIFETTEYDFETVARRLQEMAFLNKGLTINLTDERVTNEEVVDEVVSDTADAPKSAQEKAAE
SAAPHKVKHRTFHYPGGLVDFVKHINRTKNPIHQSIIDFGGKGPGHEVEIAMQWNGGYSESVHTFANTINTHEGGTHEEG
FRSALTSVVNKYAKDKKLLKDKDPNLTGDDIREGLAAVISVKVSEPQFEGQTKTKLGNTEVKSFVQKVCNEQLTHWFEAN
PADAKVIVNKAVSSAQARIAARKARELVRRKSATDLGGLPGKLADCRSTDPRKSELYVVEGDSAGGSAKSGRDSMFQAIL
PLRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDITKLRYHKIVLMADADVDGQHISTLLLTLLFRFMRPLIEHG
HVFLAQPPLYKLKWQRSDPEFAYSDRERDGLLEAGLKAGKKINKDDGIQRYKGLGEMDAKELWETTMDPTVRVLRQVTLD
DAAAADELFSILMGEDVDARRSFITRNAKDVRFLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=713, Percent_Identity=23.9831697054698, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI19913408, Length=386, Percent_Identity=28.7564766839378, Blast_Score=96, Evalue=2e-19,
Organism=Escherichia coli, GI48994957, Length=570, Percent_Identity=50.7017543859649, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1789408, Length=638, Percent_Identity=40.1253918495298, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17535065, Length=703, Percent_Identity=23.4708392603129, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI212645845, Length=638, Percent_Identity=23.9811912225705, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=259, Percent_Identity=27.7992277992278, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=216, Percent_Identity=31.4814814814815, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17136538, Length=722, Percent_Identity=24.6537396121884, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74532; Mature: 74401

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQKKKAQDEYGASAITVLEGLEAVRKRPGMYIGSTGERGLHHLIWEVVDNSVDEAMAG
CCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHC
YADRVDVRILDDGSVEVADNGRGIPVAMHATGAPTVDVVMTQLHAGGKFGGENSGYNVSG
CCCCCEEEEECCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEECCC
GLHGVGVSVVNALSTRLEVNIARDGYEWSQYYDHAVPGTLKQGEATKRTGTTIRFWADPD
CCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCC
IFETTEYDFETVARRLQEMAFLNKGLTINLTDERVTNEEVVDEVVSDTADAPKSAQEKAA
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
ESAAPHKVKHRTFHYPGGLVDFVKHINRTKNPIHQSIIDFGGKGPGHEVEIAMQWNGGYS
HHCCCCHHCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCC
ESVHTFANTINTHEGGTHEEGFRSALTSVVNKYAKDKKLLKDKDPNLTGDDIREGLAAVI
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SVKVSEPQFEGQTKTKLGNTEVKSFVQKVCNEQLTHWFEANPADAKVIVNKAVSSAQARI
EEEECCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AARKARELVRRKSATDLGGLPGKLADCRSTDPRKSELYVVEGDSAGGSAKSGRDSMFQAI
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
LPLRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDITKLRYHKIVLMADADVDGQ
HHCCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHEEEEEEEEEEECCCCCHH
HISTLLLTLLFRFMRPLIEHGHVFLAQPPLYKLKWQRSDPEFAYSDRERDGLLEAGLKAG
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHC
KKINKDDGIQRYKGLGEMDAKELWETTMDPTVRVLRQVTLDDAAAADELFSILMGEDVDA
CCCCCCHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RRSFITRNAKDVRFLDV
HHHHHHCCCCCEEEECC
>Mature Secondary Structure 
AAQKKKAQDEYGASAITVLEGLEAVRKRPGMYIGSTGERGLHHLIWEVVDNSVDEAMAG
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHC
YADRVDVRILDDGSVEVADNGRGIPVAMHATGAPTVDVVMTQLHAGGKFGGENSGYNVSG
CCCCCEEEEECCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEECCC
GLHGVGVSVVNALSTRLEVNIARDGYEWSQYYDHAVPGTLKQGEATKRTGTTIRFWADPD
CCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCC
IFETTEYDFETVARRLQEMAFLNKGLTINLTDERVTNEEVVDEVVSDTADAPKSAQEKAA
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
ESAAPHKVKHRTFHYPGGLVDFVKHINRTKNPIHQSIIDFGGKGPGHEVEIAMQWNGGYS
HHCCCCHHCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCC
ESVHTFANTINTHEGGTHEEGFRSALTSVVNKYAKDKKLLKDKDPNLTGDDIREGLAAVI
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SVKVSEPQFEGQTKTKLGNTEVKSFVQKVCNEQLTHWFEANPADAKVIVNKAVSSAQARI
EEEECCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AARKARELVRRKSATDLGGLPGKLADCRSTDPRKSELYVVEGDSAGGSAKSGRDSMFQAI
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
LPLRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDITKLRYHKIVLMADADVDGQ
HHCCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHEEEEEEEEEEECCCCCHH
HISTLLLTLLFRFMRPLIEHGHVFLAQPPLYKLKWQRSDPEFAYSDRERDGLLEAGLKAG
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHC
KKINKDDGIQRYKGLGEMDAKELWETTMDPTVRVLRQVTLDDAAAADELFSILMGEDVDA
CCCCCCHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RRSFITRNAKDVRFLDV
HHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7981652 [H]