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The map label for this gene is virB8

Identifier: 41223309

GI number: 41223309

Start: 189531

End: 190244

Strand: Direct

Name: virB8

Synonym: Atu6174

Alternate gene names: 41223309

Gene position: 189531-190244 (Clockwise)

Preceding gene: 159161998

Following gene: 16119791

Centisome position: 88.47

GC content: 54.9

Gene sequence:

>714_bases
ATGAAGGGGTCTGAATACGCCTTGCTAGTAGCGCGGGAAACCCTGGCTGAGCACTACAAGGAAGTGGAAGCTTTCCAAAC
TGCGCGTGCGAAATCAGCGCGGCGCCTCTCCAAGGTCATTGCAGCTGTCGCAACCATCGCGGTTTTGGGGAATGTTGCGC
AAGCCTTCACAATTGCCACCATGGTGCCGCTGATCAGGCTTGTGCCGGTATATCTTTGGATACGGCCGGATGGCACCGTT
GACAGCGAGGTGTCCGTCTCCCGATTGCCTGCAACTCAAGAGGAGGCCGTCGTTAACGCCTCACTGTGGGAGTATGTTCG
GCTGCGCGAGAGCTATGATGCCGATACCGCCCAGTATGCCTATGACCTGGTCTCGAACTTCAGCGCCCCAATGGTGCGCC
AAAATTATCAGCAATTCTTCAATTATCCCAATCCAACTTCGCCTCAAGTCATCCTCGGCAAACACGGCAGGCTAGAGGTC
GAACACATCGCTTCGAATGATGTTACTCCGGGTGTGCAGCAAATTCGCTATAAGCGAACCCTCATCGTTGACGGCAAAAT
GCCGATGGCGAGCACTTGGACTGCTACGGTTCGTTATGAAAAGGTGACCAGCTTGCCCGGCAGATTGAGACTGACCAACC
CGGGAGGCTTGGTTGTCACCTCCTACCAGACATCGGAAGATACCGTTTCGAACGCAGGCCACAGCGAACCATGA

Upstream 100 bases:

>100_bases
GATAAATTGGCGAGCTGCAAAGGCCCAATATTCCCGCTGAATGTGGGGCGATGGCAGCCTACGCCGTCAGATCTTCAGCT
CAGCAACGTAGGTGGTCGCC

Downstream 100 bases:

>100_bases
CGAAAAAAGCATTTCTCACTCTGGCATGTTTACTTTTTGCGGCGATTGGCGCGAGGGCTGAAGACACGCCAACGGCGGGC
AGACTTGATCCGCGCATGCG

Product: type IV secretion system protein VirB8

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MKGSEYALLVARETLAEHYKEVEAFQTARAKSARRLSKVIAAVATIAVLGNVAQAFTIATMVPLIRLVPVYLWIRPDGTV
DSEVSVSRLPATQEEAVVNASLWEYVRLRESYDADTAQYAYDLVSNFSAPMVRQNYQQFFNYPNPTSPQVILGKHGRLEV
EHIASNDVTPGVQQIRYKRTLIVDGKMPMASTWTATVRYEKVTSLPGRLRLTNPGGLVVTSYQTSEDTVSNAGHSEP

Sequences:

>Translated_237_residues
MKGSEYALLVARETLAEHYKEVEAFQTARAKSARRLSKVIAAVATIAVLGNVAQAFTIATMVPLIRLVPVYLWIRPDGTV
DSEVSVSRLPATQEEAVVNASLWEYVRLRESYDADTAQYAYDLVSNFSAPMVRQNYQQFFNYPNPTSPQVILGKHGRLEV
EHIASNDVTPGVQQIRYKRTLIVDGKMPMASTWTATVRYEKVTSLPGRLRLTNPGGLVVTSYQTSEDTVSNAGHSEP
>Mature_237_residues
MKGSEYALLVARETLAEHYKEVEAFQTARAKSARRLSKVIAAVATIAVLGNVAQAFTIATMVPLIRLVPVYLWIRPDGTV
DSEVSVSRLPATQEEAVVNASLWEYVRLRESYDADTAQYAYDLVSNFSAPMVRQNYQQFFNYPNPTSPQVILGKHGRLEV
EHIASNDVTPGVQQIRYKRTLIVDGKMPMASTWTATVRYEKVTSLPGRLRLTNPGGLVVTSYQTSEDTVSNAGHSEP

Specific function: VirB proteins are suggested to act at the bacterial surface and there play an important role in directing T-DNA transfer to plant cells

COG id: COG3736

COG function: function code U; Type IV secretory pathway, component VirB8

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virB8 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VIRB8_AGRT5 (P17798)

Other databases:

- EMBL:   X53264
- EMBL:   J03320
- EMBL:   AE007871
- PIR:   AD3249
- PIR:   S12348
- RefSeq:   NP_396495.2
- PDB:   2CC3
- PDBsum:   2CC3
- ProteinModelPortal:   P17798
- SMR:   P17798
- IntAct:   P17798
- STRING:   P17798
- GeneID:   1137497
- GenomeReviews:   AE007871_GR
- KEGG:   atu:Atu6174
- eggNOG:   COG3736
- HOGENOM:   HBG294922
- OMA:   QAFTIAT
- ProtClustDB:   PRK13865
- BioCyc:   ATUM176299-1:ATU6174-MONOMER
- InterPro:   IPR007430

Pfam domain/function: PF04335 VirB8

EC number: NA

Molecular weight: Translated: 26294; Mature: 26294

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x955debc)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGSEYALLVARETLAEHYKEVEAFQTARAKSARRLSKVIAAVATIAVLGNVAQAFTIAT
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVPLIRLVPVYLWIRPDGTVDSEVSVSRLPATQEEAVVNASLWEYVRLRESYDADTAQYA
HHHHHHHHHEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YDLVSNFSAPMVRQNYQQFFNYPNPTSPQVILGKHGRLEVEHIASNDVTPGVQQIRYKRT
HHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHEE
LIVDGKMPMASTWTATVRYEKVTSLPGRLRLTNPGGLVVTSYQTSEDTVSNAGHSEP
EEEECCCCCCCCEEEEEEEHHHHCCCCEEEEECCCCEEEEEECCCCHHHHCCCCCCH
>Mature Secondary Structure
MKGSEYALLVARETLAEHYKEVEAFQTARAKSARRLSKVIAAVATIAVLGNVAQAFTIAT
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVPLIRLVPVYLWIRPDGTVDSEVSVSRLPATQEEAVVNASLWEYVRLRESYDADTAQYA
HHHHHHHHHEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YDLVSNFSAPMVRQNYQQFFNYPNPTSPQVILGKHGRLEVEHIASNDVTPGVQQIRYKRT
HHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHEE
LIVDGKMPMASTWTATVRYEKVTSLPGRLRLTNPGGLVVTSYQTSEDTVSNAGHSEP
EEEECCCCCCCCEEEEEEEHHHHCCCCEEEEECCCCEEEEEECCCCHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2370849; 2194232; 2233252; 11743193; 11743194