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The map label for this gene is cysS

Identifier: 39998454

GI number: 39998454

Start: 3697619

End: 3699064

Strand: Reverse

Name: cysS

Synonym: GSU3365

Alternate gene names: 39998454

Gene position: 3699064-3697619 (Counterclockwise)

Preceding gene: 39998455

Following gene: 39998451

Centisome position: 96.98

GC content: 60.03

Gene sequence:

>1446_bases
ATGGCATTGCGCGTCTACAACACGCTTTCGGGCACAAAGGAAGATTTCGTCACACTTGAGCCGGGCCGGGTGAAGATGTA
TGTCTGCGGCGTCACGGTCTACGATCATTGCCACATCGGCCACGCCCGGGCCAACGTGGTCTTCGACATGATCTACCGCT
ACCTCCGCCATGCAGGCTACGAGGTGACCTATGTCCGCAACTATACCGACGTAGACGACAAGATCATCAATCGGGCCAAC
AAGGAAGGGGTGCCGTTCAACGTCATCTCCGAGCGTTTCATTGCCGAGTTCGACCGGGATATGGCGGCTCTGGGGCTCGA
TCTGCCGACCTATCAACCCAAGGCCACCGAGCACATCGCCGAGATGATCCAGGTCATCGAGCGTTTGGTGGCAAAGGGTT
TTGCCTATGCCGCCGACGGAGACGTCTACTTCAGCGTTGAGGCCTTCGATCAGTACCTGAAGCTCTCCAAGCGCAATCTT
GAGGAAATGCAGGCCGGCGCACGCATCGAGGTGGGAGAGAAAAAGCGTCACCCCATGGACTTTGCCCTCTGGAAAGGGTC
GAAGCCGGGTGAGCCCTACTGGGATTCCCCCTGGGGCCAGGGACGTCCCGGCTGGCACATCGAGTGTTCAGCCATGAGCA
TGAAGTACCTGGGCGAGACGTTCGATATTCACGGCGGCGGCAAGGATCTGGTATTTCCTCACCACGAGAACGAGATCGCC
CAGTCCGAGGCGGCCAACGGCAAGCCCTTTGCCCGCTACTGGATTCACAACGGCTTCGTGAACATCAATGCCGAGAAGAT
GAGCAAGTCCCTCGGCAACTTCTTCACCATCAAGGAAGTGCTGGAGAGCTATGACGCCGAGGTGCTCCGGTTCTTCCTCC
TGTCGGCCCACTACCGTTCCCCCATTGATTTTTCGGATCAGAACCTGAAGGAGGCCGCAGCTGGTCTGGAACGGATCTAC
AATGCCCTGGCCGGCATCGACGAGGCAGTGGCCGCAGACGGCACGGGGGCGGGGCGGATCGACGAGGAGCTGGCCGAAAA
GGCGGCCGGACTACCGACACGCTTCCGTGAGGCCATGGACGATGATTTTAACACAGCCCAGGCGTTGGGTTATGTCTTTG
ATCTGGTGCGGGCGGTCAACCGGGTGCTCGCAGAGGGTGAGATCGACCGGGCCGTACTTGCGAGCGCGCGGGAGGCAATC
GATCGGGTGGGAGCGGTGCTTGGCCTGTTCACCTCGGAGCCGGGCGCATTCGTGGAACGGCTCAAAAGCCGCAAGGCGGC
GGCTCTACCCATTGAAGCCGCTGAGATCGAGCGCCTGATCGAGGAGCGAAATGCAGCCCGCAAGGCCCGGGATTTTCGTC
GTGCCGACGAGATTCGCGATACCTTGGCGGCCCAGGGGATTTTGCTCCTCGACTCAGCCCAGGGAACAACCTGGAAGGTG
AAATAG

Upstream 100 bases:

>100_bases
ATTCGCAGCCCGGCGCGCCGGTCTTCAACCGGACCGTGACGCTGCGGGATTCGTGGGCCAAGAAGTAATACGTAGAATTA
AGAATCTGAAAGGGCTTTCC

Downstream 100 bases:

>100_bases
ATGAGCTGAAACAAAAAAGCGAATCGCCGCGGATTGATTCCGCCCGGTTCGCTTCATGTGCCGACGATGTGCGGGGCACC
CCTGGTGAGGGGTGCCCCAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MALRVYNTLSGTKEDFVTLEPGRVKMYVCGVTVYDHCHIGHARANVVFDMIYRYLRHAGYEVTYVRNYTDVDDKIINRAN
KEGVPFNVISERFIAEFDRDMAALGLDLPTYQPKATEHIAEMIQVIERLVAKGFAYAADGDVYFSVEAFDQYLKLSKRNL
EEMQAGARIEVGEKKRHPMDFALWKGSKPGEPYWDSPWGQGRPGWHIECSAMSMKYLGETFDIHGGGKDLVFPHHENEIA
QSEAANGKPFARYWIHNGFVNINAEKMSKSLGNFFTIKEVLESYDAEVLRFFLLSAHYRSPIDFSDQNLKEAAAGLERIY
NALAGIDEAVAADGTGAGRIDEELAEKAAGLPTRFREAMDDDFNTAQALGYVFDLVRAVNRVLAEGEIDRAVLASAREAI
DRVGAVLGLFTSEPGAFVERLKSRKAAALPIEAAEIERLIEERNAARKARDFRRADEIRDTLAAQGILLLDSAQGTTWKV
K

Sequences:

>Translated_481_residues
MALRVYNTLSGTKEDFVTLEPGRVKMYVCGVTVYDHCHIGHARANVVFDMIYRYLRHAGYEVTYVRNYTDVDDKIINRAN
KEGVPFNVISERFIAEFDRDMAALGLDLPTYQPKATEHIAEMIQVIERLVAKGFAYAADGDVYFSVEAFDQYLKLSKRNL
EEMQAGARIEVGEKKRHPMDFALWKGSKPGEPYWDSPWGQGRPGWHIECSAMSMKYLGETFDIHGGGKDLVFPHHENEIA
QSEAANGKPFARYWIHNGFVNINAEKMSKSLGNFFTIKEVLESYDAEVLRFFLLSAHYRSPIDFSDQNLKEAAAGLERIY
NALAGIDEAVAADGTGAGRIDEELAEKAAGLPTRFREAMDDDFNTAQALGYVFDLVRAVNRVLAEGEIDRAVLASAREAI
DRVGAVLGLFTSEPGAFVERLKSRKAAALPIEAAEIERLIEERNAARKARDFRRADEIRDTLAAQGILLLDSAQGTTWKV
K
>Mature_480_residues
ALRVYNTLSGTKEDFVTLEPGRVKMYVCGVTVYDHCHIGHARANVVFDMIYRYLRHAGYEVTYVRNYTDVDDKIINRANK
EGVPFNVISERFIAEFDRDMAALGLDLPTYQPKATEHIAEMIQVIERLVAKGFAYAADGDVYFSVEAFDQYLKLSKRNLE
EMQAGARIEVGEKKRHPMDFALWKGSKPGEPYWDSPWGQGRPGWHIECSAMSMKYLGETFDIHGGGKDLVFPHHENEIAQ
SEAANGKPFARYWIHNGFVNINAEKMSKSLGNFFTIKEVLESYDAEVLRFFLLSAHYRSPIDFSDQNLKEAAAGLERIYN
ALAGIDEAVAADGTGAGRIDEELAEKAAGLPTRFREAMDDDFNTAQALGYVFDLVRAVNRVLAEGEIDRAVLASAREAID
RVGAVLGLFTSEPGAFVERLKSRKAAALPIEAAEIERLIEERNAARKARDFRRADEIRDTLAAQGILLLDSAQGTTWKVK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=513, Percent_Identity=36.0623781676413, Blast_Score=265, Evalue=6e-71,
Organism=Homo sapiens, GI21269877, Length=320, Percent_Identity=37.1875, Blast_Score=201, Evalue=8e-52,
Organism=Homo sapiens, GI303304987, Length=320, Percent_Identity=37.1875, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI10835051, Length=320, Percent_Identity=37.1875, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI62240992, Length=320, Percent_Identity=37.1875, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1786737, Length=480, Percent_Identity=52.9166666666667, Blast_Score=487, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17509795, Length=331, Percent_Identity=35.9516616314199, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17509797, Length=331, Percent_Identity=35.6495468277946, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324082, Length=309, Percent_Identity=36.2459546925566, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI19922218, Length=502, Percent_Identity=33.6653386454183, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19922364, Length=429, Percent_Identity=32.1678321678322, Blast_Score=207, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_GEOSL (Q747A2)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954405.1
- ProteinModelPortal:   Q747A2
- GeneID:   2688225
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3365
- NMPDR:   fig|243231.1.peg.3343
- TIGR:   GSU3365
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   GSUL243231:GSU_3365-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 53913; Mature: 53781

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRVYNTLSGTKEDFVTLEPGRVKMYVCGVTVYDHCHIGHARANVVFDMIYRYLRHAGY
CEEEEEECCCCCCCCEEEECCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
EVTYVRNYTDVDDKIINRANKEGVPFNVISERFIAEFDRDMAALGLDLPTYQPKATEHIA
EEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
EMIQVIERLVAKGFAYAADGDVYFSVEAFDQYLKLSKRNLEEMQAGARIEVGEKKRHPMD
HHHHHHHHHHHCCCCEEECCCEEEEHHHHHHHHHHHHHCHHHHHCCCEEEECCCCCCCCC
FALWKGSKPGEPYWDSPWGQGRPGWHIECSAMSMKYLGETFDIHGGGKDLVFPHHENEIA
EEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCEEEECCCCCCCCCCCCCCHHH
QSEAANGKPFARYWIHNGFVNINAEKMSKSLGNFFTIKEVLESYDAEVLRFFLLSAHYRS
HHHCCCCCCHHHHEEECCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIDFSDQNLKEAAAGLERIYNALAGIDEAVAADGTGAGRIDEELAEKAAGLPTRFREAMD
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHC
DDFNTAQALGYVFDLVRAVNRVLAEGEIDRAVLASAREAIDRVGAVLGLFTSEPGAFVER
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LKSRKAAALPIEAAEIERLIEERNAARKARDFRRADEIRDTLAAQGILLLDSAQGTTWKV
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEE
K
C
>Mature Secondary Structure 
ALRVYNTLSGTKEDFVTLEPGRVKMYVCGVTVYDHCHIGHARANVVFDMIYRYLRHAGY
EEEEEECCCCCCCCEEEECCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
EVTYVRNYTDVDDKIINRANKEGVPFNVISERFIAEFDRDMAALGLDLPTYQPKATEHIA
EEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
EMIQVIERLVAKGFAYAADGDVYFSVEAFDQYLKLSKRNLEEMQAGARIEVGEKKRHPMD
HHHHHHHHHHHCCCCEEECCCEEEEHHHHHHHHHHHHHCHHHHHCCCEEEECCCCCCCCC
FALWKGSKPGEPYWDSPWGQGRPGWHIECSAMSMKYLGETFDIHGGGKDLVFPHHENEIA
EEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCEEEECCCCCCCCCCCCCCHHH
QSEAANGKPFARYWIHNGFVNINAEKMSKSLGNFFTIKEVLESYDAEVLRFFLLSAHYRS
HHHCCCCCCHHHHEEECCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIDFSDQNLKEAAAGLERIYNALAGIDEAVAADGTGAGRIDEELAEKAAGLPTRFREAMD
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHC
DDFNTAQALGYVFDLVRAVNRVLAEGEIDRAVLASAREAIDRVGAVLGLFTSEPGAFVER
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LKSRKAAALPIEAAEIERLIEERNAARKARDFRRADEIRDTLAAQGILLLDSAQGTTWKV
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEE
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA