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The map label for this gene is pol [H]

Identifier: 39998335

GI number: 39998335

Start: 3557188

End: 3559425

Strand: Reverse

Name: pol [H]

Synonym: GSU3245

Alternate gene names: 39998335

Gene position: 3559425-3557188 (Counterclockwise)

Preceding gene: 39998336

Following gene: 39998334

Centisome position: 93.32

GC content: 64.39

Gene sequence:

>2238_bases
ATGAACGACCTGGAACGGACCGACAATCGTCTCCTCTTCGGCGCCGACGACACCGCCGGGATCGTGGCAGCGGAACTGGC
CGGGCGGTTCGTCCGTCTTTTCATCCGGACTCCCCGCGGGGTCGTGGTCAGGGACGATCCCTTCCACCCGTTCATCCTCC
TGACGGACCCATCGCTCTTGGATGGATTCCGGGATGAGGTGACCTTTCACCCGCTTTCCGGACCGGGCGAGCTCCGGTTT
ATGGCACTGTTTCGCGATTGGCACGGCTGCGCCGCGGCCCGTGATTTTCTGGCCCGCCATACCGGTCAAAACCCGTCGGC
GCCCGGCGCCCCCTACCTCTATCTGTCGGACCCGGTCCACCAGCACTTTCTGCTCACAGGCAAGACCTTTTTCAAGGGAT
TTGAGTTCGGGCACCTGCGGCGGCTCGCCCTGGACATCGAGACCTCCTGCGGCGAGGGGTTCGAGTTCTCCAACCCGGAC
CGGGAAGATGACCGGATTCTCTCCATTGCTCTGATGGAGGAGGGCGGGTACGAAGCGTTCCTGTCGGCGACGGAGATGGA
CGAGCGGGAGATGCTCGAACGGCTGGGCGGGATCATCCGGGAACGTGATCCGGATGTGATCGAGGGGCACAATATCTTTC
GCTTCGACCTGGAGTACATCCGGGCACGGGCTGCCCGCCACGGGGTAAAGCTTGCCTGGGGGAGGGACGGGAGCATCCCC
CGGGTTCACCCTTCGCGCTTCTCCGTGGCTGAGCGGCTCATCGACTACCCGCGCTGGGACGTCTACGGCAGGACGGTCAT
CGATACCTACTTCCTCGTCCAGATCCACGACGTGTCGAGCAGGGATCTGGAAAGCTACGGTCTCAAGCAGGCGGCCATCC
ACTTCGGCCTGGCCGCGCCGGACCGGGTCTATCTGGACCGACAGGCCATGGACGAGGTATTCCGTTCCGATCCGGAGCGC
ATGAAGCGCTACAACCTGGACGACACCCGCGAGACTCTGGCCCTGTCCCGGCTCCTGTCCTACCCCTGGTTCCTCCAGGC
GCGCATCTTTCCCTACACCTACCAGACATCGGTCATCAGGGGGAACGCCACCAAGATCAATGCCCTCTTCCTGCGCGAGT
ACCTGCACCGGCGCGAGGCGGTGCCGCTGCCTGGTGGGGGTGACGGTGCAGGTCTTGAAGGGGGGTATGTGGAGCTACGG
GAGACCGGCATTCTCGGTCCCATTGTCCACTGTGACGTGACCTCCCTCTATCCGTCGCTCATGCTTGCCCGGAAGATCGC
TCCGGCCCGGGAGCACCTTGGTCTGTTCCTCCCCCTTCTGGAGGAGCTTCGCCGGTTCCGGCTGACAGCCAAGGCGCGCG
CCCGGGAAGAGTCAGATCCCCATCGGCGCGACTACTACGCGGCGCTCCAGCAAACCTTCAAGGTTCTCATCAACTCCTTT
TATGGCTACCTGGGCGCGCCCCTCCACCACTTTGCCGATCCGGCTGCCGCCGCCGAGGTTACCCGGGCGGGCCGGCAGCT
CATCGGGGCCATGCTTGACTGGTTACAGGACAGGGGTGCCCGCCCGGTGGAGGTGGACACGGACGGCGTCTACTTTATTC
CGCCCCCCGGCGTAAGCGGCGAAGAGGCCGAGTCCGCTCTGGTCCGCGCCCTTTCCGCTACGCTCCCGGCCGGCATTGAG
GTCGAGCTGGCCGGCCGCTACCGTGCCATGTTTTCCTATAAAGCCAAAAACTACGCTCTGCTCGGCCATGACGGCCGGAT
CACCGTGCGGGGGAGCGCCCTCCGTTCGCGGGGGATCGAGCGCTACCTGCGCGAGTTCATGGGGGAGATGATCCGCCTCC
TCCTGACGGACGAGGCGCAGAAGGTACCGGTCCTGTACGATGACTACGTCCACCGTATCCGCGAACACCGGTTTCCTGTG
GAATGGCTCGTCCGCACCGAGACCCTCGGCGAGTCGCCGGCCACGTACCAGCAGAAGGTCCGCGCCGGAAAACGCAATCC
CTCCGCGGCCTTCGAGATAGCGCTCCGCGCCGACCGGCCGTATCGGGCCGGCGATCAGATCAGCTATTACGTCACGGGAC
GGGGGAAAGGGGTAACCGTCTACGAGCAGAGCGCACCGGTCTTCCGGTACGACCCGGCCCGTCCCGACGAGAACGTGGAC
TATTATGTCGACAAGCTGGCCCAGCTCATGAAAAAATTTGCCCCCTTTCTCCCCCGGGAACGGACCCTGTTCGACTGA

Upstream 100 bases:

>100_bases
TACCTGGCAAAGCACTCCGTGGGGTAGCCTCCGGCGCAGTGCATCCGAATGAACGGCACGGTCCCACACCGTGCCGTTTG
TTTTTGGGGAGGCGCATGAC

Downstream 100 bases:

>100_bases
CGGTTTCTCCGCCATTCGCGGCCTGTTGCCGCTTGCCACCTCGGCCCAAGTGTGTTACAAGGGACAGCAGGTTGCAACCA
TGCAGCAAAAGGAGAAAACA

Product: DNA polymerase II

Products: NA

Alternate protein names: Pfu polymerase [H]

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MNDLERTDNRLLFGADDTAGIVAAELAGRFVRLFIRTPRGVVVRDDPFHPFILLTDPSLLDGFRDEVTFHPLSGPGELRF
MALFRDWHGCAAARDFLARHTGQNPSAPGAPYLYLSDPVHQHFLLTGKTFFKGFEFGHLRRLALDIETSCGEGFEFSNPD
REDDRILSIALMEEGGYEAFLSATEMDEREMLERLGGIIRERDPDVIEGHNIFRFDLEYIRARAARHGVKLAWGRDGSIP
RVHPSRFSVAERLIDYPRWDVYGRTVIDTYFLVQIHDVSSRDLESYGLKQAAIHFGLAAPDRVYLDRQAMDEVFRSDPER
MKRYNLDDTRETLALSRLLSYPWFLQARIFPYTYQTSVIRGNATKINALFLREYLHRREAVPLPGGGDGAGLEGGYVELR
ETGILGPIVHCDVTSLYPSLMLARKIAPAREHLGLFLPLLEELRRFRLTAKARAREESDPHRRDYYAALQQTFKVLINSF
YGYLGAPLHHFADPAAAAEVTRAGRQLIGAMLDWLQDRGARPVEVDTDGVYFIPPPGVSGEEAESALVRALSATLPAGIE
VELAGRYRAMFSYKAKNYALLGHDGRITVRGSALRSRGIERYLREFMGEMIRLLLTDEAQKVPVLYDDYVHRIREHRFPV
EWLVRTETLGESPATYQQKVRAGKRNPSAAFEIALRADRPYRAGDQISYYVTGRGKGVTVYEQSAPVFRYDPARPDENVD
YYVDKLAQLMKKFAPFLPRERTLFD

Sequences:

>Translated_745_residues
MNDLERTDNRLLFGADDTAGIVAAELAGRFVRLFIRTPRGVVVRDDPFHPFILLTDPSLLDGFRDEVTFHPLSGPGELRF
MALFRDWHGCAAARDFLARHTGQNPSAPGAPYLYLSDPVHQHFLLTGKTFFKGFEFGHLRRLALDIETSCGEGFEFSNPD
REDDRILSIALMEEGGYEAFLSATEMDEREMLERLGGIIRERDPDVIEGHNIFRFDLEYIRARAARHGVKLAWGRDGSIP
RVHPSRFSVAERLIDYPRWDVYGRTVIDTYFLVQIHDVSSRDLESYGLKQAAIHFGLAAPDRVYLDRQAMDEVFRSDPER
MKRYNLDDTRETLALSRLLSYPWFLQARIFPYTYQTSVIRGNATKINALFLREYLHRREAVPLPGGGDGAGLEGGYVELR
ETGILGPIVHCDVTSLYPSLMLARKIAPAREHLGLFLPLLEELRRFRLTAKARAREESDPHRRDYYAALQQTFKVLINSF
YGYLGAPLHHFADPAAAAEVTRAGRQLIGAMLDWLQDRGARPVEVDTDGVYFIPPPGVSGEEAESALVRALSATLPAGIE
VELAGRYRAMFSYKAKNYALLGHDGRITVRGSALRSRGIERYLREFMGEMIRLLLTDEAQKVPVLYDDYVHRIREHRFPV
EWLVRTETLGESPATYQQKVRAGKRNPSAAFEIALRADRPYRAGDQISYYVTGRGKGVTVYEQSAPVFRYDPARPDENVD
YYVDKLAQLMKKFAPFLPRERTLFD
>Mature_745_residues
MNDLERTDNRLLFGADDTAGIVAAELAGRFVRLFIRTPRGVVVRDDPFHPFILLTDPSLLDGFRDEVTFHPLSGPGELRF
MALFRDWHGCAAARDFLARHTGQNPSAPGAPYLYLSDPVHQHFLLTGKTFFKGFEFGHLRRLALDIETSCGEGFEFSNPD
REDDRILSIALMEEGGYEAFLSATEMDEREMLERLGGIIRERDPDVIEGHNIFRFDLEYIRARAARHGVKLAWGRDGSIP
RVHPSRFSVAERLIDYPRWDVYGRTVIDTYFLVQIHDVSSRDLESYGLKQAAIHFGLAAPDRVYLDRQAMDEVFRSDPER
MKRYNLDDTRETLALSRLLSYPWFLQARIFPYTYQTSVIRGNATKINALFLREYLHRREAVPLPGGGDGAGLEGGYVELR
ETGILGPIVHCDVTSLYPSLMLARKIAPAREHLGLFLPLLEELRRFRLTAKARAREESDPHRRDYYAALQQTFKVLINSF
YGYLGAPLHHFADPAAAAEVTRAGRQLIGAMLDWLQDRGARPVEVDTDGVYFIPPPGVSGEEAESALVRALSATLPAGIE
VELAGRYRAMFSYKAKNYALLGHDGRITVRGSALRSRGIERYLREFMGEMIRLLLTDEAQKVPVLYDDYVHRIREHRFPV
EWLVRTETLGESPATYQQKVRAGKRNPSAAFEIALRADRPYRAGDQISYYVTGRGKGVTVYEQSAPVFRYDPARPDENVD
YYVDKLAQLMKKFAPFLPRERTLFD

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=689, Percent_Identity=26.7053701015965, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786246, Length=385, Percent_Identity=25.1948051948052, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17559488, Length=693, Percent_Identity=24.3867243867244, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR004578
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 84827; Mature: 84827

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLERTDNRLLFGADDTAGIVAAELAGRFVRLFIRTPRGVVVRDDPFHPFILLTDPSLL
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEECCCCEEEECCCCCCEEEEECCHHH
DGFRDEVTFHPLSGPGELRFMALFRDWHGCAAARDFLARHTGQNPSAPGAPYLYLSDPVH
CCHHHCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCH
QHFLLTGKTFFKGFEFGHLRRLALDIETSCGEGFEFSNPDREDDRILSIALMEEGGYEAF
HHEEEECHHHHCCCCHHHHEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEH
LSATEMDEREMLERLGGIIRERDPDVIEGHNIFRFDLEYIRARAARHGVKLAWGRDGSIP
HHHHCCCHHHHHHHHHHHHHCCCCCEECCCCEEEEEHHHHHHHHHHCCCEEEECCCCCCC
RVHPSRFSVAERLIDYPRWDVYGRTVIDTYFLVQIHDVSSRDLESYGLKQAAIHFGLAAP
CCCCHHHHHHHHHHCCCCCCCCCCHHEEEEEEEEEECCCCCCHHHHCCHHHHHHHCCCCC
DRVYLDRQAMDEVFRSDPERMKRYNLDDTRETLALSRLLSYPWFLQARIFPYTYQTSVIR
CHHEECHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECEEEEEEEEE
GNATKINALFLREYLHRREAVPLPGGGDGAGLEGGYVELRETGILGPIVHCDVTSLYPSL
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHCCHHHHHHHH
MLARKIAPAREHLGLFLPLLEELRRFRLTAKARAREESDPHRRDYYAALQQTFKVLINSF
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
YGYLGAPLHHFADPAAAAEVTRAGRQLIGAMLDWLQDRGARPVEVDTDGVYFIPPPGVSG
HHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCC
EEAESALVRALSATLPAGIEVELAGRYRAMFSYKAKNYALLGHDGRITVRGSALRSRGIE
HHHHHHHHHHHHHHCCCCEEEEECCCEEEHEEECCCCEEEEECCCEEEEECHHHHHHHHH
RYLREFMGEMIRLLLTDEAQKVPVLYDDYVHRIREHRFPVEWLVRTETLGESPATYQQKV
HHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCHHHEEEHHHCCCCCHHHHHHH
RAGKRNPSAAFEIALRADRPYRAGDQISYYVTGRGKGVTVYEQSAPVFRYDPARPDENVD
HCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCEEEECCCCCCCCHH
YYVDKLAQLMKKFAPFLPRERTLFD
HHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNDLERTDNRLLFGADDTAGIVAAELAGRFVRLFIRTPRGVVVRDDPFHPFILLTDPSLL
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEECCCCEEEECCCCCCEEEEECCHHH
DGFRDEVTFHPLSGPGELRFMALFRDWHGCAAARDFLARHTGQNPSAPGAPYLYLSDPVH
CCHHHCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCH
QHFLLTGKTFFKGFEFGHLRRLALDIETSCGEGFEFSNPDREDDRILSIALMEEGGYEAF
HHEEEECHHHHCCCCHHHHEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEH
LSATEMDEREMLERLGGIIRERDPDVIEGHNIFRFDLEYIRARAARHGVKLAWGRDGSIP
HHHHCCCHHHHHHHHHHHHHCCCCCEECCCCEEEEEHHHHHHHHHHCCCEEEECCCCCCC
RVHPSRFSVAERLIDYPRWDVYGRTVIDTYFLVQIHDVSSRDLESYGLKQAAIHFGLAAP
CCCCHHHHHHHHHHCCCCCCCCCCHHEEEEEEEEEECCCCCCHHHHCCHHHHHHHCCCCC
DRVYLDRQAMDEVFRSDPERMKRYNLDDTRETLALSRLLSYPWFLQARIFPYTYQTSVIR
CHHEECHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECEEEEEEEEE
GNATKINALFLREYLHRREAVPLPGGGDGAGLEGGYVELRETGILGPIVHCDVTSLYPSL
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHCCHHHHHHHH
MLARKIAPAREHLGLFLPLLEELRRFRLTAKARAREESDPHRRDYYAALQQTFKVLINSF
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
YGYLGAPLHHFADPAAAAEVTRAGRQLIGAMLDWLQDRGARPVEVDTDGVYFIPPPGVSG
HHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCC
EEAESALVRALSATLPAGIEVELAGRYRAMFSYKAKNYALLGHDGRITVRGSALRSRGIE
HHHHHHHHHHHHHHCCCCEEEEECCCEEEHEEECCCCEEEEECCCEEEEECHHHHHHHHH
RYLREFMGEMIRLLLTDEAQKVPVLYDDYVHRIREHRFPVEWLVRTETLGESPATYQQKV
HHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCHHHEEEHHHCCCCCHHHHHHH
RAGKRNPSAAFEIALRADRPYRAGDQISYYVTGRGKGVTVYEQSAPVFRYDPARPDENVD
HCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCEEEECCCCCCCCHH
YYVDKLAQLMKKFAPFLPRERTLFD
HHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8441634; 1762925; 1579479 [H]