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The map label for this gene is rimO

Identifier: 39998295

GI number: 39998295

Start: 3511168

End: 3512511

Strand: Direct

Name: rimO

Synonym: GSU3205

Alternate gene names: 39998295

Gene position: 3511168-3512511 (Clockwise)

Preceding gene: 39998294

Following gene: 39998296

Centisome position: 92.06

GC content: 62.05

Gene sequence:

>1344_bases
GTGAGTAACGCAAAAGAAAAAGTCAGCATGGTCAGCCTTGGCTGCCCCAAAAATCTGGTGGATGCAGAGGTAATGCTCGG
CCGCCTGGCAAAGGACCGCTACGAGATCACCACCGATGAGCGCGAGGCGGACATTATCATCGTCAACACCTGTTCCTTCA
TCAAAGAGGCGAAACAGGAGAGCATCGACACGATCCTCGACCTGGCCGACCGGAAACAGGACGGCCGCTGCCGGCTCCTC
ATTGTGACCGGGTGCCTCCCCCAGCGCTACCAGGAAGAGCTGGCCAGGGAACTGCCCGAGGTGGACATCTTCGTGGGCAC
CGGCGACTATCCCCGCATCGCGGAGATCATCGAGGAGAAGAGTTCGCGGCCGGAGCAGCTGCGCTATATCGGCGACCCAA
ACTTCGTATTCGACGAAAGCCTCACGCGCCTCAACTCCTCCCCGGCCTACACCGCCTACCTGAAAATCGCCGAGGGATGC
TCCAACTGCTGCTCCTACTGCGTGATCCCGTCTCTGCGGGGAGCCTTCCGCTCCCGTCCTCTGGAGAGCGTCCTCGCTGA
GGCCCGCTCCCTCGTGGCCGGCGGGGCGCGGGAGATCAACCTGATTGCCCAGGACATCACCACCTACGGCCGCGACCTGC
CCGGCGCACCCTCCCTGGAGACCCTCATCCGCGAACTGGCCGCCATTGACGGCCTCGCCTGGATCAGACTCCTCTATGCC
TACCCCGACGGTATTACCGACGGCCTGATCCAGACCATCAAGAACGAACCAAAAGTCTGCAAATACCTGGACCTGCCCAT
CCAGCACATCAGCGACCCGATCCTGAAGCGGATGAACCGCCGCAGCACCGAGCCCCAGATCCGGGAGCTCGTGGCCAGGC
TGCGGGAGGAGATCCCGGACATCGCGCTCCGCACCTCCCTCATCGTCGGCTTCCCCGGTGAAACGGAAGAGGACTTCCGC
ACGCTCCTCCATTTCGTGGAAGAAGCCCAGTTCGACCGTCTCGGCGTGTTCTGCTATTCACGCGAAGAAGGGACCCCGGC
CGCGGAGATGCCCGACCAGGTCTCCGAGCGGGTGAAGCGCGAGCGCTACAAAAAGCTTATGAAGGCCCAGGCTCGGGTAT
CTTTCAAGCGGAACCGCCGGCTCATCGACACGGAAGAACAGGTCATCGTGGAAGGATACAGCGAGGAGACAGAGCTCCTC
CTGAAGGGACGCTCCTCCCGCCAGGCGCCCGACATCGACGGCCAGGTCTACATCACCGCCGGCAACGCCAACGTGGGTGA
CATCGTACGTCTTCGGATTACCGATTCCTCCGACTACGATCTCATCGGCGAGATCATCTCCTGA

Upstream 100 bases:

>100_bases
AAACCGCGACGACCTCCAGGATGTCATCAAGCTTCTCAAGGGAAAGGATCTGGGGGTGGAACTCCAGTTCGTCAATTTCA
GGGACTAACGAGGGGATTCA

Downstream 100 bases:

>100_bases
CCCTGCCTCCCGCGGGCCGGCGGATGACATCTGACCGGCCCGCGTCCAGCACCGCTCACTCGGCATTTCCCCGTATACTC
ACCGGCAAAGGCTGATCGGC

Product: MiaB-like tRNA modifying enzyme YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSNAKEKVSMVSLGCPKNLVDAEVMLGRLAKDRYEITTDEREADIIIVNTCSFIKEAKQESIDTILDLADRKQDGRCRLL
IVTGCLPQRYQEELARELPEVDIFVGTGDYPRIAEIIEEKSSRPEQLRYIGDPNFVFDESLTRLNSSPAYTAYLKIAEGC
SNCCSYCVIPSLRGAFRSRPLESVLAEARSLVAGGAREINLIAQDITTYGRDLPGAPSLETLIRELAAIDGLAWIRLLYA
YPDGITDGLIQTIKNEPKVCKYLDLPIQHISDPILKRMNRRSTEPQIRELVARLREEIPDIALRTSLIVGFPGETEEDFR
TLLHFVEEAQFDRLGVFCYSREEGTPAAEMPDQVSERVKRERYKKLMKAQARVSFKRNRRLIDTEEQVIVEGYSEETELL
LKGRSSRQAPDIDGQVYITAGNANVGDIVRLRITDSSDYDLIGEIIS

Sequences:

>Translated_447_residues
MSNAKEKVSMVSLGCPKNLVDAEVMLGRLAKDRYEITTDEREADIIIVNTCSFIKEAKQESIDTILDLADRKQDGRCRLL
IVTGCLPQRYQEELARELPEVDIFVGTGDYPRIAEIIEEKSSRPEQLRYIGDPNFVFDESLTRLNSSPAYTAYLKIAEGC
SNCCSYCVIPSLRGAFRSRPLESVLAEARSLVAGGAREINLIAQDITTYGRDLPGAPSLETLIRELAAIDGLAWIRLLYA
YPDGITDGLIQTIKNEPKVCKYLDLPIQHISDPILKRMNRRSTEPQIRELVARLREEIPDIALRTSLIVGFPGETEEDFR
TLLHFVEEAQFDRLGVFCYSREEGTPAAEMPDQVSERVKRERYKKLMKAQARVSFKRNRRLIDTEEQVIVEGYSEETELL
LKGRSSRQAPDIDGQVYITAGNANVGDIVRLRITDSSDYDLIGEIIS
>Mature_446_residues
SNAKEKVSMVSLGCPKNLVDAEVMLGRLAKDRYEITTDEREADIIIVNTCSFIKEAKQESIDTILDLADRKQDGRCRLLI
VTGCLPQRYQEELARELPEVDIFVGTGDYPRIAEIIEEKSSRPEQLRYIGDPNFVFDESLTRLNSSPAYTAYLKIAEGCS
NCCSYCVIPSLRGAFRSRPLESVLAEARSLVAGGAREINLIAQDITTYGRDLPGAPSLETLIRELAAIDGLAWIRLLYAY
PDGITDGLIQTIKNEPKVCKYLDLPIQHISDPILKRMNRRSTEPQIRELVARLREEIPDIALRTSLIVGFPGETEEDFRT
LLHFVEEAQFDRLGVFCYSREEGTPAAEMPDQVSERVKRERYKKLMKAQARVSFKRNRRLIDTEEQVIVEGYSEETELLL
KGRSSRQAPDIDGQVYITAGNANVGDIVRLRITDSSDYDLIGEIIS

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=28.75, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=28.75, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI93277076, Length=455, Percent_Identity=27.6923076923077, Blast_Score=169, Evalue=4e-42,
Organism=Escherichia coli, GI1787057, Length=450, Percent_Identity=39.5555555555556, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1786882, Length=448, Percent_Identity=33.0357142857143, Blast_Score=219, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17553146, Length=473, Percent_Identity=27.6955602536998, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI71996771, Length=372, Percent_Identity=27.1505376344086, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=28.9308176100629, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI19922432, Length=455, Percent_Identity=26.8131868131868, Blast_Score=149, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_GEOSL (Q747R0)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954246.1
- ProteinModelPortal:   Q747R0
- SMR:   Q747R0
- GeneID:   2688354
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3205
- NMPDR:   fig|243231.1.peg.3184
- TIGR:   GSU3205
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK829158
- BioCyc:   GSUL243231:GSU_3205-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50435; Mature: 50304

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAKEKVSMVSLGCPKNLVDAEVMLGRLAKDRYEITTDEREADIIIVNTCSFIKEAKQE
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCEEEEECHHHHHHHHHH
SIDTILDLADRKQDGRCRLLIVTGCLPQRYQEELARELPEVDIFVGTGDYPRIAEIIEEK
HHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
SSRPEQLRYIGDPNFVFDESLTRLNSSPAYTAYLKIAEGCSNCCSYCVIPSLRGAFRSRP
CCCCHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LESVLAEARSLVAGGAREINLIAQDITTYGRDLPGAPSLETLIRELAAIDGLAWIRLLYA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YPDGITDGLIQTIKNEPKVCKYLDLPIQHISDPILKRMNRRSTEPQIRELVARLREEIPD
CCCCCHHHHHHHHCCCCCHHHHHCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
IALRTSLIVGFPGETEEDFRTLLHFVEEAQFDRLGVFCYSREEGTPAAEMPDQVSERVKR
HHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHCCCHHHHHHHHH
ERYKKLMKAQARVSFKRNRRLIDTEEQVIVEGYSEETELLLKGRSSRQAPDIDGQVYITA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEE
GNANVGDIVRLRITDSSDYDLIGEIIS
CCCCCCCEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
SNAKEKVSMVSLGCPKNLVDAEVMLGRLAKDRYEITTDEREADIIIVNTCSFIKEAKQE
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCEEEEECHHHHHHHHHH
SIDTILDLADRKQDGRCRLLIVTGCLPQRYQEELARELPEVDIFVGTGDYPRIAEIIEEK
HHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
SSRPEQLRYIGDPNFVFDESLTRLNSSPAYTAYLKIAEGCSNCCSYCVIPSLRGAFRSRP
CCCCHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LESVLAEARSLVAGGAREINLIAQDITTYGRDLPGAPSLETLIRELAAIDGLAWIRLLYA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YPDGITDGLIQTIKNEPKVCKYLDLPIQHISDPILKRMNRRSTEPQIRELVARLREEIPD
CCCCCHHHHHHHHCCCCCHHHHHCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
IALRTSLIVGFPGETEEDFRTLLHFVEEAQFDRLGVFCYSREEGTPAAEMPDQVSERVKR
HHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHCCCHHHHHHHHH
ERYKKLMKAQARVSFKRNRRLIDTEEQVIVEGYSEETELLLKGRSSRQAPDIDGQVYITA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEE
GNANVGDIVRLRITDSSDYDLIGEIIS
CCCCCCCEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA