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The map label for this gene is nrfA [H]

Identifier: 39998244

GI number: 39998244

Start: 3460814

End: 3462286

Strand: Reverse

Name: nrfA [H]

Synonym: GSU3154

Alternate gene names: 39998244

Gene position: 3462286-3460814 (Counterclockwise)

Preceding gene: 39998245

Following gene: 39998243

Centisome position: 90.78

GC content: 62.12

Gene sequence:

>1473_bases
ATGAGTAAGAGAAGCATGAAGCGTTGGGCCGTCGTGACTGCCCTGGCCGCAACGGCAGCGGTGGCGGCGGCCGGAGCCGG
CTGTGCCCCCAAGAAGGTGGAACAGGCCGGCAGGGTCGCCATCCCCGACGGTACCATCGACCCGGCCGAATGGGGAAAGG
CCTACCCGGTCCACTACGATCTCTGGAAAAAGACCGAAGAGCCCACCGTGGCGGGTAAGAGCCGGTACAAGAAGGGGTAT
GACGACGACGGCGTTTTCTACGACAAGCTGGACGAGTTCCCCTTCCTGGCGCTCCTCTACAACGGTTGGGGGTTCGGCGT
GGAGTACAAGGAGCCCAGGGGCCATGCCCACATGGTGCGGGACCAGTTCGAAGTTGACCCGGGGCGAGTCAAGGCGGGAG
GCTCCTGTCTCACCTGCAAGACCCCCTATGCGCCGGCCCTGCAGCAGCGCCTCGGCAAGGACTACTTCTCCAAACCCTAC
AAGGAAGTCCATGCCCAGATCCCCAAGGAGCACCAGATGCTGGGCGTGGCCTGCATCGACTGCCACAACAACAATGACCT
GTCCCTGAAGGTCTCGCGGGGGTTCACCCTCGGCAAGGCCCTGGAAAAGCTCGGCGTGGATCAGGAGAAGCTCTCCACCC
AGGACAAGCGGACCCTGGTCTGTGCCCAGTGCCACGTCACCTACAGCATTCCCAAGGACGAGAACATGAAGTCGACCGAT
GTGTTCTTCCCCTGGGATGGCAGCAGCTATGGCGGCATTACCATCGAGAACATCATCGCAAAACTGCGGAGCAACCCGGC
AAACCGCGAGTGGACCCAAGGGGTAACCGGCTTCAAACTCGCCTTCATCCGCCACCCCGAGTTCGAGCTCTTCTCCAACA
ACAGTGTCCACTGGCAGGCGGGGGTCGCCTGCAGCGACTGCCACATGCCCTACACCAAGGTGGGCACCCGCAAGGTCTCC
GACCACCGGGTCATGAGCCCCCTCAAGAACGATCTCAAGGCGTGCCAGCAGTGCCATGCCGAGAGTCCCGAATGGCTCCG
TAATCAGGTGTACGCCATCCAGGACCGGACCATGTCCCAGTTCATCCGGGCCGGATACGCCACTGCCACCGTGGCCAAGC
TCTTCGAAATGGCCCACAAGGCCCAGGCCGAAGGCAAGCAGATCGACAAGGCCCTCTATGACCAAGCCAGGGATCACTAC
GAAGAGGCGTTCTACCGGGTAGTCTTCATCGGCGCCGAGAACTCGATCGGTTTCCATAATCCCACCGAAGCTCTGCGAAT
AACGGGCGACGCCGCTGCCCACGCGGGCAAGGCCGAAGGGTTCCTGCGGCAGGCCCTGGCCAAGGCCGGGGTGACGGTTC
CCCTCAAGGTCGACCTGGAGCTGGCCAAGTACACGAACAACCGCGGAGTGAAGAAGCTCATGTTCAAGGCCGAGCACGAG
ATCAAGGACCCGTTGGCGGCCAAGGAAAAGTAG

Upstream 100 bases:

>100_bases
GGGCTGTCACCGGCAGCTGCGGCACAAGCTGACCGGCGCCATGGCCACCCGGTGACGGGCCGGGACAGAGCATGCATTTA
GCGCAGGAGAGGAGAGCGAT

Downstream 100 bases:

>100_bases
TCACGATGGCAGCGGGGCCGGGGCCGGTATCAGTCCCTGCCGGCTCCGGTACCGCTGCTGCGCGAGGCACTACCCATGAA
CAATCGGCATCAGCATGCAC

Product: cytochrome C nitrite reductase, catalytic subunit NrfA

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSKRSMKRWAVVTALAATAAVAAAGAGCAPKKVEQAGRVAIPDGTIDPAEWGKAYPVHYDLWKKTEEPTVAGKSRYKKGY
DDDGVFYDKLDEFPFLALLYNGWGFGVEYKEPRGHAHMVRDQFEVDPGRVKAGGSCLTCKTPYAPALQQRLGKDYFSKPY
KEVHAQIPKEHQMLGVACIDCHNNNDLSLKVSRGFTLGKALEKLGVDQEKLSTQDKRTLVCAQCHVTYSIPKDENMKSTD
VFFPWDGSSYGGITIENIIAKLRSNPANREWTQGVTGFKLAFIRHPEFELFSNNSVHWQAGVACSDCHMPYTKVGTRKVS
DHRVMSPLKNDLKACQQCHAESPEWLRNQVYAIQDRTMSQFIRAGYATATVAKLFEMAHKAQAEGKQIDKALYDQARDHY
EEAFYRVVFIGAENSIGFHNPTEALRITGDAAAHAGKAEGFLRQALAKAGVTVPLKVDLELAKYTNNRGVKKLMFKAEHE
IKDPLAAKEK

Sequences:

>Translated_490_residues
MSKRSMKRWAVVTALAATAAVAAAGAGCAPKKVEQAGRVAIPDGTIDPAEWGKAYPVHYDLWKKTEEPTVAGKSRYKKGY
DDDGVFYDKLDEFPFLALLYNGWGFGVEYKEPRGHAHMVRDQFEVDPGRVKAGGSCLTCKTPYAPALQQRLGKDYFSKPY
KEVHAQIPKEHQMLGVACIDCHNNNDLSLKVSRGFTLGKALEKLGVDQEKLSTQDKRTLVCAQCHVTYSIPKDENMKSTD
VFFPWDGSSYGGITIENIIAKLRSNPANREWTQGVTGFKLAFIRHPEFELFSNNSVHWQAGVACSDCHMPYTKVGTRKVS
DHRVMSPLKNDLKACQQCHAESPEWLRNQVYAIQDRTMSQFIRAGYATATVAKLFEMAHKAQAEGKQIDKALYDQARDHY
EEAFYRVVFIGAENSIGFHNPTEALRITGDAAAHAGKAEGFLRQALAKAGVTVPLKVDLELAKYTNNRGVKKLMFKAEHE
IKDPLAAKEK
>Mature_489_residues
SKRSMKRWAVVTALAATAAVAAAGAGCAPKKVEQAGRVAIPDGTIDPAEWGKAYPVHYDLWKKTEEPTVAGKSRYKKGYD
DDGVFYDKLDEFPFLALLYNGWGFGVEYKEPRGHAHMVRDQFEVDPGRVKAGGSCLTCKTPYAPALQQRLGKDYFSKPYK
EVHAQIPKEHQMLGVACIDCHNNNDLSLKVSRGFTLGKALEKLGVDQEKLSTQDKRTLVCAQCHVTYSIPKDENMKSTDV
FFPWDGSSYGGITIENIIAKLRSNPANREWTQGVTGFKLAFIRHPEFELFSNNSVHWQAGVACSDCHMPYTKVGTRKVSD
HRVMSPLKNDLKACQQCHAESPEWLRNQVYAIQDRTMSQFIRAGYATATVAKLFEMAHKAQAEGKQIDKALYDQARDHYE
EAFYRVVFIGAENSIGFHNPTEALRITGDAAAHAGKAEGFLRQALAKAGVTVPLKVDLELAKYTNNRGVKKLMFKAEHEI
KDPLAAKEK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=393, Percent_Identity=29.2620865139949, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 54601; Mature: 54469

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRSMKRWAVVTALAATAAVAAAGAGCAPKKVEQAGRVAIPDGTIDPAEWGKAYPVHYD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEECCCCCCCHHHHCCCCCEEEH
LWKKTEEPTVAGKSRYKKGYDDDGVFYDKLDEFPFLALLYNGWGFGVEYKEPRGHAHMVR
HHCCCCCCCCCCHHHHCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCEECCCCCCHHHHH
DQFEVDPGRVKAGGSCLTCKTPYAPALQQRLGKDYFSKPYKEVHAQIPKEHQMLGVACID
HHHCCCCCCEECCCCEEEECCCCCHHHHHHHCHHHHHCCHHHHHHHCCHHHHEEEEEEEE
CHNNNDLSLKVSRGFTLGKALEKLGVDQEKLSTQDKRTLVCAQCHVTYSIPKDENMKSTD
ECCCCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCE
VFFPWDGSSYGGITIENIIAKLRSNPANREWTQGVTGFKLAFIRHPEFELFSNNSVHWQA
EEECCCCCCCCCEEHHHHHHHHHCCCCCCHHHCCCCCEEEEEEECCCEEEECCCCEEEEE
GVACSDCHMPYTKVGTRKVSDHRVMSPLKNDLKACQQCHAESPEWLRNQVYAIQDRTMSQ
CCEECCCCCCHHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEHHHHHHHHH
FIRAGYATATVAKLFEMAHKAQAEGKQIDKALYDQARDHYEEAFYRVVFIGAENSIGFHN
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCC
PTEALRITGDAAAHAGKAEGFLRQALAKAGVTVPLKVDLELAKYTNNRGVKKLMFKAEHE
CCCCEEEECHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEEEEHHCCCCCHHHHHHHHHHH
IKDPLAAKEK
HCCCCCCCCC
>Mature Secondary Structure 
SKRSMKRWAVVTALAATAAVAAAGAGCAPKKVEQAGRVAIPDGTIDPAEWGKAYPVHYD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEECCCCCCCHHHHCCCCCEEEH
LWKKTEEPTVAGKSRYKKGYDDDGVFYDKLDEFPFLALLYNGWGFGVEYKEPRGHAHMVR
HHCCCCCCCCCCHHHHCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCEECCCCCCHHHHH
DQFEVDPGRVKAGGSCLTCKTPYAPALQQRLGKDYFSKPYKEVHAQIPKEHQMLGVACID
HHHCCCCCCEECCCCEEEECCCCCHHHHHHHCHHHHHCCHHHHHHHCCHHHHEEEEEEEE
CHNNNDLSLKVSRGFTLGKALEKLGVDQEKLSTQDKRTLVCAQCHVTYSIPKDENMKSTD
ECCCCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCE
VFFPWDGSSYGGITIENIIAKLRSNPANREWTQGVTGFKLAFIRHPEFELFSNNSVHWQA
EEECCCCCCCCCEEHHHHHHHHHCCCCCCHHHCCCCCEEEEEEECCCEEEECCCCEEEEE
GVACSDCHMPYTKVGTRKVSDHRVMSPLKNDLKACQQCHAESPEWLRNQVYAIQDRTMSQ
CCEECCCCCCHHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEHHHHHHHHH
FIRAGYATATVAKLFEMAHKAQAEGKQIDKALYDQARDHYEEAFYRVVFIGAENSIGFHN
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCC
PTEALRITGDAAAHAGKAEGFLRQALAKAGVTVPLKVDLELAKYTNNRGVKKLMFKAEHE
CCCCEEEECHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEEEEHHCCCCCHHHHHHHHHHH
IKDPLAAKEK
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA