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The map label for this gene is murD

Identifier: 39998162

GI number: 39998162

Start: 3377285

End: 3378643

Strand: Reverse

Name: murD

Synonym: GSU3071

Alternate gene names: 39998162

Gene position: 3378643-3377285 (Counterclockwise)

Preceding gene: 39998163

Following gene: 308513336

Centisome position: 88.58

GC content: 65.42

Gene sequence:

>1359_bases
ATGGACCTCAAAGGAAAGAACATACTCGTTGTGGGCCTCGCCCGGACCGGCGTGGCGGTTGCCCGTTTCCTCGCGGCGCA
GGGCGCCCGGGTGACCGTGACCGATTTGCGGGACGAGGCCGCCCTGGCCGGGCCCCTGGAGCAACTGGCCGGCCACCCTG
TGAGCTACGTGCTGGGACGTCACGACGAGGCCGACTTCGCTGCCGCCGAAACGGTGGTGGTAAGCCCGGGCGTCCCCCAG
GAAAGCCCCTATCTCCAGGCGGCACGACGGGCCGGACGTGAGGTCATTACCGAGATCGAGCTTGCGTCCAGGTTCATCAC
TGCGCCTCTGGTGGCCATCACGGGCACCAACGGCAAGACGACCACCACGACCCTCACCGGCGAACTCTTCGCGGGATGCG
GGTTCCGGACGTTCGTTGGAGGAAATATCGGCAACCCGCTCATCGAACTGGTGGAGACCGGGGCGGCAGTGGACCGGGTG
GTGGTGGAGATCAGCTCCTTCCAGCTCGAATGGATCCGCTCGTTCCGGCCCACGGTCGCGGTGCTCCTCAACATCACCGA
AGACCACCTGGACCGGTACGCCACCTATCAGGAGTACATCGACGCCAAGGCCCGGATTTTCAAGAACCAGACCGCGTCGG
ACTGGGCGGTCCTCAACGTGGACGATCCGATCGTGGCAGGTCTGGCCGGCAGGACGTGCGCTGCAGTGTTCCCCATGAGC
CGGCAACGGGAGCTTGGCGAGGGGATATTCTATCGGGACGGGGCCATCATCTTCCGCCACGCCGGTCGCCAGGAGCGGTT
TCCCACGGACCGCTTCAGGATCACCGGGGTCCACAACATTGAGAACATCATGGCGTCCCTTGCCGCCGCGCTGCTTCTGG
GATGCGATGCCGACCGGGCGTTGGCGTGCGTTGAGGCGTTCGGCGGACTCCCCCACCGGATGGAACTGGTGCGCGAACTC
AACGGTGTCCGGTACTTCGAGGACAGCAAGGCCACCAACGTGGGGAGCGTGGAAAAGGCGCTGGCTAGCTTCAACGACAT
CACCCTCATCGCCGGCGGCAAAGACAAGGGGGGCAGCTATGCCCCCCTCGCCGGACTCGTGGCCGAGCGCGTACGGCATA
TGATCCTGATCGGCGAGGCAAAGGATCGAATGGCCCGTGAACTGGGGCATCTCACGGACACCCGCATGGCAGTAACCCTG
GAAGATGCCGTGGAACTGGCCGCCTCACTGACGCAGCCGGGGGGAGTGGTCCTCTTCTCGCCCGCCTGTTCCAGCTTCGA
CATGTTCCGCGATTACGAAGAGCGGGCGCAGCGGTTCCGCGCCGCCGTTGATGCGCTGAGTCCGGGAGGCACGCCGTGA

Upstream 100 bases:

>100_bases
GAACTGAAAGGGGTGGCGGAGCCGAAGATCATCGTCAGGTTCTGGATCATCACCATCATTCTTGCGCTAGTCGCCATTTC
GACGCTGAAGCTGAGATAGC

Downstream 100 bases:

>100_bases
ACCTGGGCCTGAACGTAAAGGAGATCGAACGCTACGACCTCGTGATCCTCCTCATGGCGGTGGCTCTCACCTGCTTCGGG
GTGGTGATGGTCTACTCGGC

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MDLKGKNILVVGLARTGVAVARFLAAQGARVTVTDLRDEAALAGPLEQLAGHPVSYVLGRHDEADFAAAETVVVSPGVPQ
ESPYLQAARRAGREVITEIELASRFITAPLVAITGTNGKTTTTTLTGELFAGCGFRTFVGGNIGNPLIELVETGAAVDRV
VVEISSFQLEWIRSFRPTVAVLLNITEDHLDRYATYQEYIDAKARIFKNQTASDWAVLNVDDPIVAGLAGRTCAAVFPMS
RQRELGEGIFYRDGAIIFRHAGRQERFPTDRFRITGVHNIENIMASLAAALLLGCDADRALACVEAFGGLPHRMELVREL
NGVRYFEDSKATNVGSVEKALASFNDITLIAGGKDKGGSYAPLAGLVAERVRHMILIGEAKDRMARELGHLTDTRMAVTL
EDAVELAASLTQPGGVVLFSPACSSFDMFRDYEERAQRFRAAVDALSPGGTP

Sequences:

>Translated_452_residues
MDLKGKNILVVGLARTGVAVARFLAAQGARVTVTDLRDEAALAGPLEQLAGHPVSYVLGRHDEADFAAAETVVVSPGVPQ
ESPYLQAARRAGREVITEIELASRFITAPLVAITGTNGKTTTTTLTGELFAGCGFRTFVGGNIGNPLIELVETGAAVDRV
VVEISSFQLEWIRSFRPTVAVLLNITEDHLDRYATYQEYIDAKARIFKNQTASDWAVLNVDDPIVAGLAGRTCAAVFPMS
RQRELGEGIFYRDGAIIFRHAGRQERFPTDRFRITGVHNIENIMASLAAALLLGCDADRALACVEAFGGLPHRMELVREL
NGVRYFEDSKATNVGSVEKALASFNDITLIAGGKDKGGSYAPLAGLVAERVRHMILIGEAKDRMARELGHLTDTRMAVTL
EDAVELAASLTQPGGVVLFSPACSSFDMFRDYEERAQRFRAAVDALSPGGTP
>Mature_452_residues
MDLKGKNILVVGLARTGVAVARFLAAQGARVTVTDLRDEAALAGPLEQLAGHPVSYVLGRHDEADFAAAETVVVSPGVPQ
ESPYLQAARRAGREVITEIELASRFITAPLVAITGTNGKTTTTTLTGELFAGCGFRTFVGGNIGNPLIELVETGAAVDRV
VVEISSFQLEWIRSFRPTVAVLLNITEDHLDRYATYQEYIDAKARIFKNQTASDWAVLNVDDPIVAGLAGRTCAAVFPMS
RQRELGEGIFYRDGAIIFRHAGRQERFPTDRFRITGVHNIENIMASLAAALLLGCDADRALACVEAFGGLPHRMELVREL
NGVRYFEDSKATNVGSVEKALASFNDITLIAGGKDKGGSYAPLAGLVAERVRHMILIGEAKDRMARELGHLTDTRMAVTL
EDAVELAASLTQPGGVVLFSPACSSFDMFRDYEERAQRFRAAVDALSPGGTP

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=448, Percent_Identity=37.2767857142857, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_GEOSL (Q748D4)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954113.1
- ProteinModelPortal:   Q748D4
- GeneID:   2686310
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3071
- NMPDR:   fig|243231.1.peg.3051
- TIGR:   GSU3071
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   CLSK829090
- BioCyc:   GSUL243231:GSU_3071-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48680; Mature: 48680

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKGKNILVVGLARTGVAVARFLAAQGARVTVTDLRDEAALAGPLEQLAGHPVSYVLGR
CCCCCCCEEEEEECHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCHHHHCCCCHHHHHCC
HDEADFAAAETVVVSPGVPQESPYLQAARRAGREVITEIELASRFITAPLVAITGTNGKT
CCCCCHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCE
TTTTLTGELFAGCGFRTFVGGNIGNPLIELVETGAAVDRVVVEISSFQLEWIRSFRPTVA
EEEEEEHHHHHCCCCHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEE
VLLNITEDHLDRYATYQEYIDAKARIFKNQTASDWAVLNVDDPIVAGLAGRTCAAVFPMS
HEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCHHHHHCCCH
RQRELGEGIFYRDGAIIFRHAGRQERFPTDRFRITGVHNIENIMASLAAALLLGCDADRA
HHHHHCCCEEEECCEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHH
LACVEAFGGLPHRMELVRELNGVRYFEDSKATNVGSVEKALASFNDITLIAGGKDKGGSY
HHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCC
APLAGLVAERVRHMILIGEAKDRMARELGHLTDTRMAVTLEDAVELAASLTQPGGVVLFS
CHHHHHHHHHHHHEEEEECHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCEEEEC
PACSSFDMFRDYEERAQRFRAAVDALSPGGTP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDLKGKNILVVGLARTGVAVARFLAAQGARVTVTDLRDEAALAGPLEQLAGHPVSYVLGR
CCCCCCCEEEEEECHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCHHHHCCCCHHHHHCC
HDEADFAAAETVVVSPGVPQESPYLQAARRAGREVITEIELASRFITAPLVAITGTNGKT
CCCCCHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCE
TTTTLTGELFAGCGFRTFVGGNIGNPLIELVETGAAVDRVVVEISSFQLEWIRSFRPTVA
EEEEEEHHHHHCCCCHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEE
VLLNITEDHLDRYATYQEYIDAKARIFKNQTASDWAVLNVDDPIVAGLAGRTCAAVFPMS
HEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCHHHHHCCCH
RQRELGEGIFYRDGAIIFRHAGRQERFPTDRFRITGVHNIENIMASLAAALLLGCDADRA
HHHHHCCCEEEECCEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHH
LACVEAFGGLPHRMELVRELNGVRYFEDSKATNVGSVEKALASFNDITLIAGGKDKGGSY
HHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCC
APLAGLVAERVRHMILIGEAKDRMARELGHLTDTRMAVTLEDAVELAASLTQPGGVVLFS
CHHHHHHHHHHHHEEEEECHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCEEEEC
PACSSFDMFRDYEERAQRFRAAVDALSPGGTP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA