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The map label for this gene is irlS [H]

Identifier: 39998038

GI number: 39998038

Start: 3244341

End: 3245768

Strand: Direct

Name: irlS [H]

Synonym: GSU2947

Alternate gene names: 39998038

Gene position: 3244341-3245768 (Clockwise)

Preceding gene: 39998037

Following gene: 39998039

Centisome position: 85.06

GC content: 63.87

Gene sequence:

>1428_bases
ATGTACTTGAAGCGAAGTGACGGCCCCTCCCCGGGAACGCTCTCCCTTGCCGCGCGTCTCACCGTCCTCTCCACCCTCGC
CACCACCGGCGTCCTCCTCTTTGCCATCGTGTTTCAGTTCCTTGCCCTGGTAAGCGACCTGGAGTTCGAGGACAACGACT
TCATCATCGACAAGATCCGGGTTATCGAGGCGATCATCGTCCGTTACCCCGAAGACAGAGTGCACCTTGAGCAGGAAGTC
CAGTGGGAAGGGAGCATGCGCGAGGATAGCCGGTACCTGGTCCGCATCGCCGACGCCCGGAAAAATATCATAATGGAGAC
GCGGGGAATGGACCGCATTGCCCCGCCCGCCATGTTTCCCCGGCCGGCCCGGGACACGCACGCCATCGGCCGGGGGAAGA
AGCTCAAGACCCCCGAGGGCCGCGTCTACCTGGTAAACGCAGCCTGGACCGACGGGGGACGAGCGCCGGACAGCCGCCAG
ATCCAGGTGGCGCTGGACGTAACCGAAGAAGAGGAGATGCTTGAAGGATACAAGCAGAAGATGGCTTTTGTGTTTCTGGC
CGGACTCTTTCTCTCGGCAGGTCTCAACGTGGTGGTGGTGCGACGGGGGCTGCGCCCCCTGGTCCAACTCACGGAGACGG
CTGGCCGCATTGACGTGACAACCCTCCACGAACGGATCGATGCCCGGGTATGGCCTCGTGAGCTGGCCCGTTTCGCCGCC
GCCTTCGACGCGATGCTCACCCGGCTCGAATCCTCCTTTGAGCGGCTTGAATCCTCCTCCGCCAACCTGGCCCACGAAAT
CCGCACCCCCCTCAACATCCTGCGGGGCGAAGCGGAAGTGACCCTCTCCCGGGCCCGTTCTCCGGAAGAATACCGCCGGG
TCATCGAGTCGAGCCTTGAGGAGTACGAGCGGCTCTCGCGGCTCATCGACAACATTCTCTTCCTGGCCCGGGCCGAGCAG
CGGATCGAACCGGTCCCCCTGGACGCGGGCCATGAACTGGAACTGCTTCAGGACTACTACGGCACCCTGGCAGAGGAAAA
GGGAATCGCCATCTCGTGCACCGGCGAAGGAACGATTTCGGCCGATCCGCTCCTGTTCCAACGGGCCGTCGGGAACGTCC
TCTCCAACGCCATCCGCTACACCCCAGCAGGGGGAACGATTACCGTCAGCATCGATCGGGCCGATGACGGCGCCGCACTC
CTGACCGTCATCGACACGGGCATCGGTATCTCCGCGGACGACCTCCCACGGGTCTTCGACCGGTTCTACCGCAGCAGCGA
GGCCCGTACCCTCAATGCCCAGGGAACCGGGCTCGGCCTCGCCATCGTTCGTTCTATCATGGAACTTCACCGGGGGAGCG
TGACCATCGATAGCGCTCCGGGCAGGGGGACCGCCGTGACGCTCCGCTTCCCACTCGCCAGCCAGTAG

Upstream 100 bases:

>100_bases
AGCGACACTAACATCGTCGACGTAGCGATCCGTCGGCTACGGCGCAAGGTCGACGACCCCTTCGAGAGGAAGCTCATCCA
CACCGTCCGCGGAGCAGGCT

Downstream 100 bases:

>100_bases
TTTTTCTTTTCTTTCGAACTACCATTGGAGGCTCCCGAAGGGGACGGAGAGAGATCCTCAGCCACCGCCTCGCCACGCTC
CAGTTACACCAGGATGACAA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MYLKRSDGPSPGTLSLAARLTVLSTLATTGVLLFAIVFQFLALVSDLEFEDNDFIIDKIRVIEAIIVRYPEDRVHLEQEV
QWEGSMREDSRYLVRIADARKNIIMETRGMDRIAPPAMFPRPARDTHAIGRGKKLKTPEGRVYLVNAAWTDGGRAPDSRQ
IQVALDVTEEEEMLEGYKQKMAFVFLAGLFLSAGLNVVVVRRGLRPLVQLTETAGRIDVTTLHERIDARVWPRELARFAA
AFDAMLTRLESSFERLESSSANLAHEIRTPLNILRGEAEVTLSRARSPEEYRRVIESSLEEYERLSRLIDNILFLARAEQ
RIEPVPLDAGHELELLQDYYGTLAEEKGIAISCTGEGTISADPLLFQRAVGNVLSNAIRYTPAGGTITVSIDRADDGAAL
LTVIDTGIGISADDLPRVFDRFYRSSEARTLNAQGTGLGLAIVRSIMELHRGSVTIDSAPGRGTAVTLRFPLASQ

Sequences:

>Translated_475_residues
MYLKRSDGPSPGTLSLAARLTVLSTLATTGVLLFAIVFQFLALVSDLEFEDNDFIIDKIRVIEAIIVRYPEDRVHLEQEV
QWEGSMREDSRYLVRIADARKNIIMETRGMDRIAPPAMFPRPARDTHAIGRGKKLKTPEGRVYLVNAAWTDGGRAPDSRQ
IQVALDVTEEEEMLEGYKQKMAFVFLAGLFLSAGLNVVVVRRGLRPLVQLTETAGRIDVTTLHERIDARVWPRELARFAA
AFDAMLTRLESSFERLESSSANLAHEIRTPLNILRGEAEVTLSRARSPEEYRRVIESSLEEYERLSRLIDNILFLARAEQ
RIEPVPLDAGHELELLQDYYGTLAEEKGIAISCTGEGTISADPLLFQRAVGNVLSNAIRYTPAGGTITVSIDRADDGAAL
LTVIDTGIGISADDLPRVFDRFYRSSEARTLNAQGTGLGLAIVRSIMELHRGSVTIDSAPGRGTAVTLRFPLASQ
>Mature_475_residues
MYLKRSDGPSPGTLSLAARLTVLSTLATTGVLLFAIVFQFLALVSDLEFEDNDFIIDKIRVIEAIIVRYPEDRVHLEQEV
QWEGSMREDSRYLVRIADARKNIIMETRGMDRIAPPAMFPRPARDTHAIGRGKKLKTPEGRVYLVNAAWTDGGRAPDSRQ
IQVALDVTEEEEMLEGYKQKMAFVFLAGLFLSAGLNVVVVRRGLRPLVQLTETAGRIDVTTLHERIDARVWPRELARFAA
AFDAMLTRLESSFERLESSSANLAHEIRTPLNILRGEAEVTLSRARSPEEYRRVIESSLEEYERLSRLIDNILFLARAEQ
RIEPVPLDAGHELELLQDYYGTLAEEKGIAISCTGEGTISADPLLFQRAVGNVLSNAIRYTPAGGTITVSIDRADDGAAL
LTVIDTGIGISADDLPRVFDRFYRSSEARTLNAQGTGLGLAIVRSIMELHRGSVTIDSAPGRGTAVTLRFPLASQ

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=478, Percent_Identity=28.8702928870293, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788279, Length=301, Percent_Identity=35.5481727574751, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1790861, Length=240, Percent_Identity=34.1666666666667, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788393, Length=246, Percent_Identity=30.0813008130081, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=29.3040293040293, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI145693157, Length=219, Percent_Identity=31.9634703196347, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786600, Length=219, Percent_Identity=30.5936073059361, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1788713, Length=222, Percent_Identity=29.7297297297297, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=32.2727272727273, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788549, Length=219, Percent_Identity=29.6803652968037, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1786912, Length=234, Percent_Identity=31.1965811965812, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789403, Length=307, Percent_Identity=28.3387622149837, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI48994928, Length=219, Percent_Identity=28.310502283105, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=216, Percent_Identity=27.3148148148148, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787894, Length=283, Percent_Identity=29.3286219081272, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1789808, Length=307, Percent_Identity=25.4071661237785, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=222, Percent_Identity=28.8288288288288, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790551, Length=304, Percent_Identity=24.6710526315789, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87082128, Length=221, Percent_Identity=24.4343891402715, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52652; Mature: 52652

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN ; PS01088 CAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLKRSDGPSPGTLSLAARLTVLSTLATTGVLLFAIVFQFLALVSDLEFEDNDFIIDKIR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
VIEAIIVRYPEDRVHLEQEVQWEGSMREDSRYLVRIADARKNIIMETRGMDRIAPPAMFP
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEEHHHCCCEEECCCCCCCCCCCCCC
RPARDTHAIGRGKKLKTPEGRVYLVNAAWTDGGRAPDSRQIQVALDVTEEEEMLEGYKQK
CCCCCHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
MAFVFLAGLFLSAGLNVVVVRRGLRPLVQLTETAGRIDVTTLHERIDARVWPRELARFAA
HHHHHHHHHHHHCCCCEEEEHHCHHHHHHHHHHCCCEEHHHHHHHHCCCCCHHHHHHHHH
AFDAMLTRLESSFERLESSSANLAHEIRTPLNILRGEAEVTLSRARSPEEYRRVIESSLE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHH
EYERLSRLIDNILFLARAEQRIEPVPLDAGHELELLQDYYGTLAEEKGIAISCTGEGTIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCC
ADPLLFQRAVGNVLSNAIRYTPAGGTITVSIDRADDGAALLTVIDTGIGISADDLPRVFD
CCHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHH
RFYRSSEARTLNAQGTGLGLAIVRSIMELHRGSVTIDSAPGRGTAVTLRFPLASQ
HHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MYLKRSDGPSPGTLSLAARLTVLSTLATTGVLLFAIVFQFLALVSDLEFEDNDFIIDKIR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
VIEAIIVRYPEDRVHLEQEVQWEGSMREDSRYLVRIADARKNIIMETRGMDRIAPPAMFP
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEEHHHCCCEEECCCCCCCCCCCCCC
RPARDTHAIGRGKKLKTPEGRVYLVNAAWTDGGRAPDSRQIQVALDVTEEEEMLEGYKQK
CCCCCHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHH
MAFVFLAGLFLSAGLNVVVVRRGLRPLVQLTETAGRIDVTTLHERIDARVWPRELARFAA
HHHHHHHHHHHHCCCCEEEEHHCHHHHHHHHHHCCCEEHHHHHHHHCCCCCHHHHHHHHH
AFDAMLTRLESSFERLESSSANLAHEIRTPLNILRGEAEVTLSRARSPEEYRRVIESSLE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHH
EYERLSRLIDNILFLARAEQRIEPVPLDAGHELELLQDYYGTLAEEKGIAISCTGEGTIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCC
ADPLLFQRAVGNVLSNAIRYTPAGGTITVSIDRADDGAALLTVIDTGIGISADDLPRVFD
CCHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHH
RFYRSSEARTLNAQGTGLGLAIVRSIMELHRGSVTIDSAPGRGTAVTLRFPLASQ
HHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]