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The map label for this gene is amy [H]

Identifier: 39997730

GI number: 39997730

Start: 2905877

End: 2907730

Strand: Reverse

Name: amy [H]

Synonym: GSU2636

Alternate gene names: 39997730

Gene position: 2907730-2905877 (Counterclockwise)

Preceding gene: 39997731

Following gene: 39997729

Centisome position: 76.24

GC content: 64.67

Gene sequence:

>1854_bases
ATGACGGCACCGCAATCGATCAGGGACCTGGATCTGGAACGCCTCTGCAATCGCACCTTTCACCCTTCCCCGTCAGCCTG
GGAGGACGAGGTGCTCTACTTCCTGCTGCTCGACCGCTTTTCCGACGGCAACGAGACGGGCTACCGGGACAACCGGGGAC
GGCGCACGAACCGGGGCGCCACCCCGCTGTACCGCCCGGCCGATGCGGGCAACGCCATCGCCACCGAGCCCGAGGCAGCG
GCCTGGCGCGAGGCCGGCACCCGCTGGTGCGGCGGGAACCTGAAGGGCCTCATGGACAAGATGGGGTATCTCCGGCGATT
GGGGGTGACCGCGGTCTGGGTCAGCCCCCTGTTCAAGCAGTGCTCATTTGTCCCCACCTACCACGGTTACGGTATCCAGA
ACTTCCTGGACGTGGACCCCCACTTCGGCACTCGCGACGACCTGCGGGAACTAGTCCGCGTGGCCCACGCCAACGGCATT
TACGTCATCCTCGACATCATTCTCAATCATGCGGGGAACGTGTTCGCCTACGACCGGGGATACACCCCCTACGACGGCGG
CACCAGCTACCCGGTGACGGGATTCCGCACGGCCGACGGCACTCCGTCGCTTCCCTTCGGCCCCGTCGGTTCACCGGCCC
CCGCCGAAGCCTGGCCCGACGGTGCGGTCTGGCCCCGGGAACTGCAGGCGCCGGAGTGCTTCACGCGCAAAGGATACATC
CGGGATTGGGACCGTTATCACGAATACCTGGATGGTGATTTCTTCGACCTGAAGGATATTCACCTGGGCGACGGGGACGT
GGATTCGTTCAGTCCGTCCCCGGCGCTCCTGGCACTTTGCGAGGTGTACAAGTACTGGATCGCCTACGCCGACCTGGACG
GCTACCGGATAGACACCGTAAAGCATATGGGCCCCGGGGCCACCCGTTTTTTCGCCTCCACCATCCACGAATTCGCCCAG
AGGATCGGCAAGGAGAATTTCTATCTCATCGGTGAGATCACCGGCGGCCGGGTCAATGCCTTCGACACCCTGGAGACCAC
CGGCCTCAACGCCGCGCTGGGGGTGGACGACATTCCGCTGCGCCTGGAAGATCTGGTGAAGGGAAAGGCCAACCCGGCCG
ACTACTTCGATCTCTTCCGCAACTCCCTGCTGGTGCGCAAGGAGTCCCACATCTGGTTCCGCGACAAGGTGGTGACGGTT
ATCGACGACCACGACCAGGTCTGCCGCGGCGCCAAGAAAGAGCGGTTCTGCGCCGGCGATCCCACGTGGCGCAAGCTGGT
GCCAAACGCCCTGGCCCTCAATGCCACCACCCTCGGCATCCCCTGCATCTACTACGGTACCGAGCAGGCCTTTGACGGCG
AAGGGGGGGACGACCGCTACCTGCGCGAATGCATGTTCGGCGGCCCCTTCGGCGCCTTTCGCAGCCGCGGGGTCCACTTC
TTCGACGAGGATAATCCGGTCTACCGGGAGTGTGCCAAGGTCCTTGCCCTGCGCCGTGACATCATCGCCCTGCGCCGGGG
GCGCCAGTATCTGCGGGAGATCTCCGGCGATGGAGTGAACTTCGGGCTTCCCTACGTCATCGGTGGCGAGATCCGCTCCG
TGGTGGCCTGGTCGCGGATCTTCGACACCGAAGAGATAGTTCTCGCCATCAACACCGACCCTGACCATCCGCGCACCGCA
CTGGTGCTCGTGGACGGCTCCCTTCACCGGCCGGGGGACGTTTTCTTCTGCCGCTACTCGACGGACCCGGGTCAGGTCCG
TCGAAGGGTGAGGGTGCGGGATGCCGGTCCCGCCATTCGGGCCGTAGAGCTTACGGTGCCGGCGGCCGGCTTCGTACTGT
TCCAGAAGCGATAA

Upstream 100 bases:

>100_bases
GCTGAAGATCAGCTAACGTTCGATTCACCGGCTGTTTCAGGCCCTCTCCCCTTGTCGGGAGAGGGCTTTTCTCTTTCAGC
GGAACAAGGAGGATCACGCC

Downstream 100 bases:

>100_bases
TACAATCGGGATAATGGCCCCGCTTTTGCTTTGAACGACATGGTGCGAGCCATTTTTTCAGAAGGAGGGGCCATGTGGAC
CGATGATTTGACAGTGGGCA

Product: alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MTAPQSIRDLDLERLCNRTFHPSPSAWEDEVLYFLLLDRFSDGNETGYRDNRGRRTNRGATPLYRPADAGNAIATEPEAA
AWREAGTRWCGGNLKGLMDKMGYLRRLGVTAVWVSPLFKQCSFVPTYHGYGIQNFLDVDPHFGTRDDLRELVRVAHANGI
YVILDIILNHAGNVFAYDRGYTPYDGGTSYPVTGFRTADGTPSLPFGPVGSPAPAEAWPDGAVWPRELQAPECFTRKGYI
RDWDRYHEYLDGDFFDLKDIHLGDGDVDSFSPSPALLALCEVYKYWIAYADLDGYRIDTVKHMGPGATRFFASTIHEFAQ
RIGKENFYLIGEITGGRVNAFDTLETTGLNAALGVDDIPLRLEDLVKGKANPADYFDLFRNSLLVRKESHIWFRDKVVTV
IDDHDQVCRGAKKERFCAGDPTWRKLVPNALALNATTLGIPCIYYGTEQAFDGEGGDDRYLRECMFGGPFGAFRSRGVHF
FDEDNPVYRECAKVLALRRDIIALRRGRQYLREISGDGVNFGLPYVIGGEIRSVVAWSRIFDTEEIVLAINTDPDHPRTA
LVLVDGSLHRPGDVFFCRYSTDPGQVRRRVRVRDAGPAIRAVELTVPAAGFVLFQKR

Sequences:

>Translated_617_residues
MTAPQSIRDLDLERLCNRTFHPSPSAWEDEVLYFLLLDRFSDGNETGYRDNRGRRTNRGATPLYRPADAGNAIATEPEAA
AWREAGTRWCGGNLKGLMDKMGYLRRLGVTAVWVSPLFKQCSFVPTYHGYGIQNFLDVDPHFGTRDDLRELVRVAHANGI
YVILDIILNHAGNVFAYDRGYTPYDGGTSYPVTGFRTADGTPSLPFGPVGSPAPAEAWPDGAVWPRELQAPECFTRKGYI
RDWDRYHEYLDGDFFDLKDIHLGDGDVDSFSPSPALLALCEVYKYWIAYADLDGYRIDTVKHMGPGATRFFASTIHEFAQ
RIGKENFYLIGEITGGRVNAFDTLETTGLNAALGVDDIPLRLEDLVKGKANPADYFDLFRNSLLVRKESHIWFRDKVVTV
IDDHDQVCRGAKKERFCAGDPTWRKLVPNALALNATTLGIPCIYYGTEQAFDGEGGDDRYLRECMFGGPFGAFRSRGVHF
FDEDNPVYRECAKVLALRRDIIALRRGRQYLREISGDGVNFGLPYVIGGEIRSVVAWSRIFDTEEIVLAINTDPDHPRTA
LVLVDGSLHRPGDVFFCRYSTDPGQVRRRVRVRDAGPAIRAVELTVPAAGFVLFQKR
>Mature_616_residues
TAPQSIRDLDLERLCNRTFHPSPSAWEDEVLYFLLLDRFSDGNETGYRDNRGRRTNRGATPLYRPADAGNAIATEPEAAA
WREAGTRWCGGNLKGLMDKMGYLRRLGVTAVWVSPLFKQCSFVPTYHGYGIQNFLDVDPHFGTRDDLRELVRVAHANGIY
VILDIILNHAGNVFAYDRGYTPYDGGTSYPVTGFRTADGTPSLPFGPVGSPAPAEAWPDGAVWPRELQAPECFTRKGYIR
DWDRYHEYLDGDFFDLKDIHLGDGDVDSFSPSPALLALCEVYKYWIAYADLDGYRIDTVKHMGPGATRFFASTIHEFAQR
IGKENFYLIGEITGGRVNAFDTLETTGLNAALGVDDIPLRLEDLVKGKANPADYFDLFRNSLLVRKESHIWFRDKVVTVI
DDHDQVCRGAKKERFCAGDPTWRKLVPNALALNATTLGIPCIYYGTEQAFDGEGGDDRYLRECMFGGPFGAFRSRGVHFF
DEDNPVYRECAKVLALRRDIIALRRGRQYLREISGDGVNFGLPYVIGGEIRSVVAWSRIFDTEEIVLAINTDPDHPRTAL
VLVDGSLHRPGDVFFCRYSTDPGQVRRRVRVRDAGPAIRAVELTVPAAGFVLFQKR

Specific function: May Play A Role In Regulating The Intracellular Level Of Maltotriose. Cleaves Glucose From The Reducing End Of Maltotriose And Longer Maltodextrins With A Chain Length Of Up To 7 Glucose Units. [C]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=546, Percent_Identity=23.6263736263736, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789995, Length=174, Percent_Identity=32.7586206896552, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790687, Length=282, Percent_Identity=24.468085106383, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322245, Length=405, Percent_Identity=23.7037037037037, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24586587, Length=214, Percent_Identity=28.0373831775701, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24586599, Length=82, Percent_Identity=40.2439024390244, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24586597, Length=100, Percent_Identity=36, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221330053, Length=82, Percent_Identity=39.0243902439024, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24586591, Length=84, Percent_Identity=40.4761904761905, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586593, Length=82, Percent_Identity=40.2439024390244, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005323 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF03714 PUD [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 69291; Mature: 69160

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPQSIRDLDLERLCNRTFHPSPSAWEDEVLYFLLLDRFSDGNETGYRDNRGRRTNRGA
CCCCCCHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TPLYRPADAGNAIATEPEAAAWREAGTRWCGGNLKGLMDKMGYLRRLGVTAVWVSPLFKQ
CCCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHH
CSFVPTYHGYGIQNFLDVDPHFGTRDDLRELVRVAHANGIYVILDIILNHAGNVFAYDRG
CCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHCCCCEEEECCC
YTPYDGGTSYPVTGFRTADGTPSLPFGPVGSPAPAEAWPDGAVWPRELQAPECFTRKGYI
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHCCCCCC
RDWDRYHEYLDGDFFDLKDIHLGDGDVDSFSPSPALLALCEVYKYWIAYADLDGYRIDTV
CCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEE
KHMGPGATRFFASTIHEFAQRIGKENFYLIGEITGGRVNAFDTLETTGLNAALGVDDIPL
HHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCHHHCCCCEECCCCCCCC
RLEDLVKGKANPADYFDLFRNSLLVRKESHIWFRDKVVTVIDDHDQVCRGAKKERFCAGD
HHHHHHCCCCCCHHHHHHHHHHEEEEECCCEEEECEEEEEECCCHHHHCCCCCCCCCCCC
PTWRKLVPNALALNATTLGIPCIYYGTEQAFDGEGGDDRYLRECMFGGPFGAFRSRGVHF
CHHHHHCCCHHEECCEEECCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCEE
FDEDNPVYRECAKVLALRRDIIALRRGRQYLREISGDGVNFGLPYVIGGEIRSVVAWSRI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHH
FDTEEIVLAINTDPDHPRTALVLVDGSLHRPGDVFFCRYSTDPGQVRRRVRVRDAGPAIR
CCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHEEECCCCCEE
AVELTVPAAGFVLFQKR
EEEEECCCCEEEEEECC
>Mature Secondary Structure 
TAPQSIRDLDLERLCNRTFHPSPSAWEDEVLYFLLLDRFSDGNETGYRDNRGRRTNRGA
CCCCCHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
TPLYRPADAGNAIATEPEAAAWREAGTRWCGGNLKGLMDKMGYLRRLGVTAVWVSPLFKQ
CCCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHH
CSFVPTYHGYGIQNFLDVDPHFGTRDDLRELVRVAHANGIYVILDIILNHAGNVFAYDRG
CCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHCCCCEEEECCC
YTPYDGGTSYPVTGFRTADGTPSLPFGPVGSPAPAEAWPDGAVWPRELQAPECFTRKGYI
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHCCCCCC
RDWDRYHEYLDGDFFDLKDIHLGDGDVDSFSPSPALLALCEVYKYWIAYADLDGYRIDTV
CCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEE
KHMGPGATRFFASTIHEFAQRIGKENFYLIGEITGGRVNAFDTLETTGLNAALGVDDIPL
HHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCHHHCCCCEECCCCCCCC
RLEDLVKGKANPADYFDLFRNSLLVRKESHIWFRDKVVTVIDDHDQVCRGAKKERFCAGD
HHHHHHCCCCCCHHHHHHHHHHEEEEECCCEEEECEEEEEECCCHHHHCCCCCCCCCCCC
PTWRKLVPNALALNATTLGIPCIYYGTEQAFDGEGGDDRYLRECMFGGPFGAFRSRGVHF
CHHHHHCCCHHEECCEEECCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCEE
FDEDNPVYRECAKVLALRRDIIALRRGRQYLREISGDGVNFGLPYVIGGEIRSVVAWSRI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHH
FDTEEIVLAINTDPDHPRTALVLVDGSLHRPGDVFFCRYSTDPGQVRRRVRVRDAGPAIR
CCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHEEECCCCCEE
AVELTVPAAGFVLFQKR
EEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8424949; 8485150 [H]