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The map label for this gene is sseA [C]

Identifier: 39997612

GI number: 39997612

Start: 2775205

End: 2777202

Strand: Reverse

Name: sseA [C]

Synonym: GSU2517

Alternate gene names: 39997612

Gene position: 2777202-2775205 (Counterclockwise)

Preceding gene: 39997613

Following gene: 39997611

Centisome position: 72.81

GC content: 68.92

Gene sequence:

>1998_bases
ATGAGTCATTCCCCCTGGTCCGCCGGTGACGTGGCGACGCGTTGCACCGCCTGCGGCATCTGCGTAGCCGAATGCGCCTT
CCTGCGGGAGCACGGCACGCCAAAGGCGCTGGCAGCATCCTTTGACCCCCGGCGACTGGACCAGTCCCATCTCCCCTTCG
GGTGCAGCCTGTGCGGCCTCTGCCGCGCGGTCTGCCCCGAAGGGGTCGATCCTGCCGCCCTGTTCCTTGCCATGCGGCGC
GCCACCGCCGATGCCGGTACGCTGCAACTGTCTCCCTACCGGGGGCTCCTTTCCTACGAGCGGGCCGGGACATCGAGACT
TTTCACCTGGTACGGGCTCCCCGACGGGTGCCGTACGATCTTTTTCCCCGGCTGCGCGCTGCCGGGGACCCGCCCCCGGA
CAACGCTGAAGCTCTTCGACCTGCTGCGCGGCAGCGAACCATCCCTCGGCATGGTTCTCGACTGTTGCACCAAGCCGTCC
CACGACCTGGGACGATCCGGGCACTTTACGGCCATGTTCGGCGAAATGAAGGCCTACCTGCTGGCGCAGGGGGTGAAGAC
CGTCCAGGTGGCCTGTCCCAACTGCCATGAGGTGTTCTCCCGCTATGCCCCGGAGCTGGAGGTGGTCACCGTTTACGAGG
TACTGGCGCGGGCGGGCATCAGCTTCAAACAGACCGAGGGCGCTGTGACCGTGCACGATCCCTGCGTCTCGCGCGATAAC
GCCGGGGTGCACGCGGCCGTGAGAACGCTGCTGGGCGCAGCCGGGCTCAGGGTTCGGGAAATGCCCCACCACGGCGTCCG
GACCCTCTGCTGCGGCGAGGGAGGAGCGGTCCGCTGCACCGCGCCCCGCCTGGCCGGGGCATGGACCTCCCTGCGCGCGG
CAGAGGCCGGCGGCAGCACGACGATCACCTACTGTGCCGGCTGCGCCTCGTATCTCGGTCCGCACACGCCCACCAGCCAC
GTGGCGGACCTGCTTTTCGAACCTGAGGCCGCCCTGGCGGGCAGGGTCAAGGTGGCCAGCGGTCCCCGGACCTACCTGAA
CCGGTTGCTGCTGAAGAGGCGGTTGCGCCGCAGCCTCCCGGTGGCCTCGTCGCGGGAGCGGACCTTCCGCAGCGCTGCCG
ATGGCGTCGGCAGCGCTGTCGGCGGGAGACGCGCCGCCGTACTCATCCTCCTGATGCTGGCGGCGGCTGTCCTTGTGGTG
ATGCTTGCCGTTTCCGGTGCGTCAGCCCGCGATTTCGGGCTGATGGAGCCCCAGACCCTGGCGCGCGCCCCCGGAACCTG
GGTGGTGCTCGACGGCCGTCCGCGCTCCGACTGGCAGGCGGGTCACATCCCCGGCGCCCGTTCCTTTTCGTGGGAGAACT
ACACCAGAACGGACGACAAGGGGGTCCCCTACAAGCTCTGGCCGCCCCGCGACCTCGCCCGGGCCCTGGGATCCATGGGG
ATCGACGAGCGGACTCCCGTGGTGGTGTACGGGGATGCGGACAAGAGCTGGGGAGGGGAGGGCTGGGCCGCCTGGGTCCT
GGCCTGGCTCGGGCACAAGGGCCCCGTGCGGATGCTCAACGGCGGCATCCAGGCCTGGACCGCCGCGGGACTTCCCCTCA
CGCGAGGCCATGAACGGTACAGCGGCGGCACCCTGACGTACCGCTCTGCCGTTCGGCCCGAGGAAGATATTACCACCGCC
GAACTGGAGCGTTCGCTCGGCGCGGTCACGATTATCGATACCCGCTCCTTTTTCGAGCGGCTCGCCGGACGTATCCCAGG
CTCGGTGCACATCCCCTGGAACAAGTTCTTTACGGGCAAGGAGCGTCGCCCCCTGGCTCCGGCCGAGGTGCGCAGGCTTC
TGGCCAGGAACGGGGTCGATGCGGACCGGCCCGTTGTCTATTACTGTGCCGGGGGAATCCGGTCGGCGTACGCCCTGATG
GTGCACGAACTGGCCGGCCTGGGCCCTGCCCGCAATTACGAGGGGGGCATGGAGGAGTGGAAAAGGCTGCACCGCTGA

Upstream 100 bases:

>100_bases
ATGACTACCTGAAGTCCCGCGGCGTGGATGAGAAGCGGCTCTTCATCCTTGAAAAAGGAATGGAGGGGTGGCCCCATCCC
CAGCTCTGCGAATCGGGCAA

Downstream 100 bases:

>100_bases
TCCGCCGCGGCCCCCGGTCCGGCCGAAGCGATCCGTCTGATGTTGTGTCGCCCCCGCGCGGGGTTATGAAATCCGGGCGT
TCCTGCCCGAGCCTGTCACC

Product: rhodanese-like domain/cysteine-rich domain-containing protein

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MSHSPWSAGDVATRCTACGICVAECAFLREHGTPKALAASFDPRRLDQSHLPFGCSLCGLCRAVCPEGVDPAALFLAMRR
ATADAGTLQLSPYRGLLSYERAGTSRLFTWYGLPDGCRTIFFPGCALPGTRPRTTLKLFDLLRGSEPSLGMVLDCCTKPS
HDLGRSGHFTAMFGEMKAYLLAQGVKTVQVACPNCHEVFSRYAPELEVVTVYEVLARAGISFKQTEGAVTVHDPCVSRDN
AGVHAAVRTLLGAAGLRVREMPHHGVRTLCCGEGGAVRCTAPRLAGAWTSLRAAEAGGSTTITYCAGCASYLGPHTPTSH
VADLLFEPEAALAGRVKVASGPRTYLNRLLLKRRLRRSLPVASSRERTFRSAADGVGSAVGGRRAAVLILLMLAAAVLVV
MLAVSGASARDFGLMEPQTLARAPGTWVVLDGRPRSDWQAGHIPGARSFSWENYTRTDDKGVPYKLWPPRDLARALGSMG
IDERTPVVVYGDADKSWGGEGWAAWVLAWLGHKGPVRMLNGGIQAWTAAGLPLTRGHERYSGGTLTYRSAVRPEEDITTA
ELERSLGAVTIIDTRSFFERLAGRIPGSVHIPWNKFFTGKERRPLAPAEVRRLLARNGVDADRPVVYYCAGGIRSAYALM
VHELAGLGPARNYEGGMEEWKRLHR

Sequences:

>Translated_665_residues
MSHSPWSAGDVATRCTACGICVAECAFLREHGTPKALAASFDPRRLDQSHLPFGCSLCGLCRAVCPEGVDPAALFLAMRR
ATADAGTLQLSPYRGLLSYERAGTSRLFTWYGLPDGCRTIFFPGCALPGTRPRTTLKLFDLLRGSEPSLGMVLDCCTKPS
HDLGRSGHFTAMFGEMKAYLLAQGVKTVQVACPNCHEVFSRYAPELEVVTVYEVLARAGISFKQTEGAVTVHDPCVSRDN
AGVHAAVRTLLGAAGLRVREMPHHGVRTLCCGEGGAVRCTAPRLAGAWTSLRAAEAGGSTTITYCAGCASYLGPHTPTSH
VADLLFEPEAALAGRVKVASGPRTYLNRLLLKRRLRRSLPVASSRERTFRSAADGVGSAVGGRRAAVLILLMLAAAVLVV
MLAVSGASARDFGLMEPQTLARAPGTWVVLDGRPRSDWQAGHIPGARSFSWENYTRTDDKGVPYKLWPPRDLARALGSMG
IDERTPVVVYGDADKSWGGEGWAAWVLAWLGHKGPVRMLNGGIQAWTAAGLPLTRGHERYSGGTLTYRSAVRPEEDITTA
ELERSLGAVTIIDTRSFFERLAGRIPGSVHIPWNKFFTGKERRPLAPAEVRRLLARNGVDADRPVVYYCAGGIRSAYALM
VHELAGLGPARNYEGGMEEWKRLHR
>Mature_664_residues
SHSPWSAGDVATRCTACGICVAECAFLREHGTPKALAASFDPRRLDQSHLPFGCSLCGLCRAVCPEGVDPAALFLAMRRA
TADAGTLQLSPYRGLLSYERAGTSRLFTWYGLPDGCRTIFFPGCALPGTRPRTTLKLFDLLRGSEPSLGMVLDCCTKPSH
DLGRSGHFTAMFGEMKAYLLAQGVKTVQVACPNCHEVFSRYAPELEVVTVYEVLARAGISFKQTEGAVTVHDPCVSRDNA
GVHAAVRTLLGAAGLRVREMPHHGVRTLCCGEGGAVRCTAPRLAGAWTSLRAAEAGGSTTITYCAGCASYLGPHTPTSHV
ADLLFEPEAALAGRVKVASGPRTYLNRLLLKRRLRRSLPVASSRERTFRSAADGVGSAVGGRRAAVLILLMLAAAVLVVM
LAVSGASARDFGLMEPQTLARAPGTWVVLDGRPRSDWQAGHIPGARSFSWENYTRTDDKGVPYKLWPPRDLARALGSMGI
DERTPVVVYGDADKSWGGEGWAAWVLAWLGHKGPVRMLNGGIQAWTAAGLPLTRGHERYSGGTLTYRSAVRPEEDITTAE
LERSLGAVTIIDTRSFFERLAGRIPGSVHIPWNKFFTGKERRPLAPAEVRRLLARNGVDADRPVVYYCAGGIRSAYALMV
HELAGLGPARNYEGGMEEWKRLHR

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=241, Percent_Identity=25.7261410788382, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI61835204, Length=241, Percent_Identity=25.7261410788382, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI194473681, Length=241, Percent_Identity=25.7261410788382, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI87082121, Length=238, Percent_Identity=24.7899159663866, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 71782; Mature: 71651

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50206 RHODANESE_3 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSPWSAGDVATRCTACGICVAECAFLREHGTPKALAASFDPRRLDQSHLPFGCSLCGL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCCCHHHHHH
CRAVCPEGVDPAALFLAMRRATADAGTLQLSPYRGLLSYERAGTSRLFTWYGLPDGCRTI
HHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCEE
FFPGCALPGTRPRTTLKLFDLLRGSEPSLGMVLDCCTKPSHDLGRSGHFTAMFGEMKAYL
EECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHCCCCCCEEHHHHHHHHHH
LAQGVKTVQVACPNCHEVFSRYAPELEVVTVYEVLARAGISFKQTEGAVTVHDPCVSRDN
HHCCCCEEEEECCCHHHHHHHHCCCEEEHHHHHHHHHCCCCEEECCCCEEEECCCCCCCC
AGVHAAVRTLLGAAGLRVREMPHHGVRTLCCGEGGAVRCTAPRLAGAWTSLRAAEAGGST
CCHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCC
TITYCAGCASYLGPHTPTSHVADLLFEPEAALAGRVKVASGPRTYLNRLLLKRRLRRSLP
EEHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCEEECCCCHHHHHHHHHHHHHHHHCC
VASSRERTFRSAADGVGSAVGGRRAAVLILLMLAAAVLVVMLAVSGASARDFGLMEPQTL
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
ARAPGTWVVLDGRPRSDWQAGHIPGARSFSWENYTRTDDKGVPYKLWPPRDLARALGSMG
HCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCC
IDERTPVVVYGDADKSWGGEGWAAWVLAWLGHKGPVRMLNGGIQAWTAAGLPLTRGHERY
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCCCCCCCCCC
SGGTLTYRSAVRPEEDITTAELERSLGAVTIIDTRSFFERLAGRIPGSVHIPWNKFFTGK
CCCCEEECCCCCCCCCCHHHHHHHHCCCEEEEECHHHHHHHHHCCCCEEECCHHHCCCCC
ERRPLAPAEVRRLLARNGVDADRPVVYYCAGGIRSAYALMVHELAGLGPARNYEGGMEEW
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
KRLHR
HHHCC
>Mature Secondary Structure 
SHSPWSAGDVATRCTACGICVAECAFLREHGTPKALAASFDPRRLDQSHLPFGCSLCGL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCCCHHHHHH
CRAVCPEGVDPAALFLAMRRATADAGTLQLSPYRGLLSYERAGTSRLFTWYGLPDGCRTI
HHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCEE
FFPGCALPGTRPRTTLKLFDLLRGSEPSLGMVLDCCTKPSHDLGRSGHFTAMFGEMKAYL
EECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHCCCCCCEEHHHHHHHHHH
LAQGVKTVQVACPNCHEVFSRYAPELEVVTVYEVLARAGISFKQTEGAVTVHDPCVSRDN
HHCCCCEEEEECCCHHHHHHHHCCCEEEHHHHHHHHHCCCCEEECCCCEEEECCCCCCCC
AGVHAAVRTLLGAAGLRVREMPHHGVRTLCCGEGGAVRCTAPRLAGAWTSLRAAEAGGST
CCHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCC
TITYCAGCASYLGPHTPTSHVADLLFEPEAALAGRVKVASGPRTYLNRLLLKRRLRRSLP
EEHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCEEECCCCHHHHHHHHHHHHHHHHCC
VASSRERTFRSAADGVGSAVGGRRAAVLILLMLAAAVLVVMLAVSGASARDFGLMEPQTL
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
ARAPGTWVVLDGRPRSDWQAGHIPGARSFSWENYTRTDDKGVPYKLWPPRDLARALGSMG
HCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCC
IDERTPVVVYGDADKSWGGEGWAAWVLAWLGHKGPVRMLNGGIQAWTAAGLPLTRGHERY
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCHHHHHCCCCCCCCCCCCC
SGGTLTYRSAVRPEEDITTAELERSLGAVTIIDTRSFFERLAGRIPGSVHIPWNKFFTGK
CCCCEEECCCCCCCCCCHHHHHHHHCCCEEEEECHHHHHHHHHCCCCEEECCHHHCCCCC
ERRPLAPAEVRRLLARNGVDADRPVVYYCAGGIRSAYALMVHELAGLGPARNYEGGMEEW
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
KRLHR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]