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The map label for this gene is waaA [H]

Identifier: 39997357

GI number: 39997357

Start: 2473676

End: 2474980

Strand: Reverse

Name: waaA [H]

Synonym: GSU2259

Alternate gene names: 39997357

Gene position: 2474980-2473676 (Counterclockwise)

Preceding gene: 39997358

Following gene: 39997356

Centisome position: 64.89

GC content: 64.9

Gene sequence:

>1305_bases
ATGGTCTTTCTTGTGTACGACATACTGCTGCTCCTGCTGTCGCCCGCGATTATCGCCCACCACGCCTGGCGGACAGTGAG
CCGGGGGAGGAGTTTCGCCGGGTTCGGCGAGCGGTTCGGGTTCATCGCGCCGGAACGGCTCATACCCGTTGCGGGCAAGA
GGCCCGTCTGGGTCCACGCGGTTTCGGTGGGGGAAACCATGGCGGTGAAGCCCCTGCTGCGTGAGCTGAAACATCGGTAT
CCCGAGCGGCCCGTCGTGCTCTCAACCGTGACCGAAACCGGACGTTCTATTGCCGAGAAGATCGCCGAGGCGGATCTAGT
CGTCTACTTCCCCTTTGATTTCGGCTTTGCCGTGTCCCGCGCACTTCGGCTCGTATCCCCGTCGCTGGTCATTGTGGTGG
AGACGGAGATCTGGCCCAATTTCCTGCGCTACGCCCGTCGGAGGTCAATCCCCGCCGTCATGGTCAATGGTCGCATTTCG
GACCGCTCCTTTCCGCGCTATCTCCGGTTTTCGTGGTTTTTTGCCCCACTCCTGGCCAAGCTGTCCGCCCTCTGCATGCA
GAGCGAGGAGGATGCCCGCCGCATCGTGGCTATCGGTGGGCCGGCGCAGCGGGTGTTCGTGACACGCAACCTCAAGTATG
ATCTCCCCGTGAGAACCCTGAGCCCGGCGGAGCGCGAGGAATTGCACCAGCGTTACCGTCTCCCACCGGACACCCTCGTC
ATTACTGCCGGAAGTACCCATGCCGGTGAGGAAGAGGTCGTGGTGGACAGCTATGCCCGCCTTGCCCGGGAGCGGTCCGG
ACTGTTCCTGGTGCTCGTGCCCCGGCACCCGGAGCGCGCCGCTGAAGTAGGGACGATGCTGCAGGGCAAAGGGATTCCCC
ATGTGCGTCGTTCCGCCCTTGATGGGGCGCCTGAACCGGCAGCCGGCGGGGTTCTGCTGGTGGACACCGTCGGCGAACTC
ATGAACCTCTATGCCCTGTCCGACTTGGTCTTCGTGGGGGGAAGCCTCGTGCCGGTGGGCGGGCACAACCTGCTGGAACC
TGCGTCGGTCGGGGCCCCGGTCCTGTTCGGCCCCCACATGCACAATTTTCGGGAGATAACGGCCCTGGTCCTGGCAGCCA
ATGCGGGCGAGCAGGTTGAAGACCGAGCCGGTTTGGAGGATGCCCTGCGGCGGCTCCTGAACGACGAGCCCCGCCGACTC
GCCATGGGTGAGCGCGGCATCCGCCTCATGACAGAGCAAGGGGGGGCCGCCGCCCGTCACCTGGAGATCATCGGGCGCCT
GCTTGTCACGGGGAGCGCCGAGTGA

Upstream 100 bases:

>100_bases
GCGCGACGGGCTCTACCGCAAGCTCTACGACATGCAGTTCAAGTGAAAGGCCGGGACCGGTCCCGGGTTTGACGGCCGAC
CGGTCGGGGATGGCGTTTCG

Downstream 100 bases:

>100_bases
GTTGCACGGGGTTCTGGCGAAAACTGGCTTCCGGTGAAGAGTGTGGCGCGGCTGCCCGGCTTCTCGGCGCGGTTCTCGCC
ATCCCAGCCGCCCTCTATGG

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MVFLVYDILLLLLSPAIIAHHAWRTVSRGRSFAGFGERFGFIAPERLIPVAGKRPVWVHAVSVGETMAVKPLLRELKHRY
PERPVVLSTVTETGRSIAEKIAEADLVVYFPFDFGFAVSRALRLVSPSLVIVVETEIWPNFLRYARRRSIPAVMVNGRIS
DRSFPRYLRFSWFFAPLLAKLSALCMQSEEDARRIVAIGGPAQRVFVTRNLKYDLPVRTLSPAEREELHQRYRLPPDTLV
ITAGSTHAGEEEVVVDSYARLARERSGLFLVLVPRHPERAAEVGTMLQGKGIPHVRRSALDGAPEPAAGGVLLVDTVGEL
MNLYALSDLVFVGGSLVPVGGHNLLEPASVGAPVLFGPHMHNFREITALVLAANAGEQVEDRAGLEDALRRLLNDEPRRL
AMGERGIRLMTEQGGAAARHLEIIGRLLVTGSAE

Sequences:

>Translated_434_residues
MVFLVYDILLLLLSPAIIAHHAWRTVSRGRSFAGFGERFGFIAPERLIPVAGKRPVWVHAVSVGETMAVKPLLRELKHRY
PERPVVLSTVTETGRSIAEKIAEADLVVYFPFDFGFAVSRALRLVSPSLVIVVETEIWPNFLRYARRRSIPAVMVNGRIS
DRSFPRYLRFSWFFAPLLAKLSALCMQSEEDARRIVAIGGPAQRVFVTRNLKYDLPVRTLSPAEREELHQRYRLPPDTLV
ITAGSTHAGEEEVVVDSYARLARERSGLFLVLVPRHPERAAEVGTMLQGKGIPHVRRSALDGAPEPAAGGVLLVDTVGEL
MNLYALSDLVFVGGSLVPVGGHNLLEPASVGAPVLFGPHMHNFREITALVLAANAGEQVEDRAGLEDALRRLLNDEPRRL
AMGERGIRLMTEQGGAAARHLEIIGRLLVTGSAE
>Mature_434_residues
MVFLVYDILLLLLSPAIIAHHAWRTVSRGRSFAGFGERFGFIAPERLIPVAGKRPVWVHAVSVGETMAVKPLLRELKHRY
PERPVVLSTVTETGRSIAEKIAEADLVVYFPFDFGFAVSRALRLVSPSLVIVVETEIWPNFLRYARRRSIPAVMVNGRIS
DRSFPRYLRFSWFFAPLLAKLSALCMQSEEDARRIVAIGGPAQRVFVTRNLKYDLPVRTLSPAEREELHQRYRLPPDTLV
ITAGSTHAGEEEVVVDSYARLARERSGLFLVLVPRHPERAAEVGTMLQGKGIPHVRRSALDGAPEPAAGGVLLVDTVGEL
MNLYALSDLVFVGGSLVPVGGHNLLEPASVGAPVLFGPHMHNFREITALVLAANAGEQVEDRAGLEDALRRLLNDEPRRL
AMGERGIRLMTEQGGAAARHLEIIGRLLVTGSAE

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=430, Percent_Identity=37.906976744186, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47852; Mature: 47852

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFLVYDILLLLLSPAIIAHHAWRTVSRGRSFAGFGERFGFIAPERLIPVAGKRPVWVHA
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEECCCCCCCEEEEE
VSVGETMAVKPLLRELKHRYPERPVVLSTVTETGRSIAEKIAEADLVVYFPFDFGFAVSR
EECCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
ALRLVSPSLVIVVETEIWPNFLRYARRRSIPAVMVNGRISDRSFPRYLRFSWFFAPLLAK
HHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECEECCCCCCHHHHHHHHHHHHHHH
LSALCMQSEEDARRIVAIGGPAQRVFVTRNLKYDLPVRTLSPAEREELHQRYRLPPDTLV
HHHHHHCCCCCCCEEEEECCCCEEEEEEECCEECCCCCCCCCHHHHHHHHHHCCCCCCEE
ITAGSTHAGEEEVVVDSYARLARERSGLFLVLVPRHPERAAEVGTMLQGKGIPHVRRSAL
EEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHC
DGAPEPAAGGVLLVDTVGELMNLYALSDLVFVGGSLVPVGGHNLLEPASVGAPVLFGPHM
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHCCCCEEECCCC
HNFREITALVLAANAGEQVEDRAGLEDALRRLLNDEPRRLAMGERGIRLMTEQGGAAARH
HHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCEECCCCCCCEEEECCCCHHHHH
LEIIGRLLVTGSAE
HHHHHHHHHCCCCC
>Mature Secondary Structure
MVFLVYDILLLLLSPAIIAHHAWRTVSRGRSFAGFGERFGFIAPERLIPVAGKRPVWVHA
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEECCCCCCCEEEEE
VSVGETMAVKPLLRELKHRYPERPVVLSTVTETGRSIAEKIAEADLVVYFPFDFGFAVSR
EECCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
ALRLVSPSLVIVVETEIWPNFLRYARRRSIPAVMVNGRISDRSFPRYLRFSWFFAPLLAK
HHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECEECCCCCCHHHHHHHHHHHHHHH
LSALCMQSEEDARRIVAIGGPAQRVFVTRNLKYDLPVRTLSPAEREELHQRYRLPPDTLV
HHHHHHCCCCCCCEEEEECCCCEEEEEEECCEECCCCCCCCCHHHHHHHHHHCCCCCCEE
ITAGSTHAGEEEVVVDSYARLARERSGLFLVLVPRHPERAAEVGTMLQGKGIPHVRRSAL
EEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHC
DGAPEPAAGGVLLVDTVGELMNLYALSDLVFVGGSLVPVGGHNLLEPASVGAPVLFGPHM
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHCCCCEEECCCC
HNFREITALVLAANAGEQVEDRAGLEDALRRLLNDEPRRLAMGERGIRLMTEQGGAAARH
HHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCEECCCCCCCEEEECCCCHHHHH
LEIIGRLLVTGSAE
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]