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The map label for this gene is capI [H]

Identifier: 39997339

GI number: 39997339

Start: 2455686

End: 2456696

Strand: Reverse

Name: capI [H]

Synonym: GSU2241

Alternate gene names: 39997339

Gene position: 2456696-2455686 (Counterclockwise)

Preceding gene: 39997340

Following gene: 39997338

Centisome position: 64.41

GC content: 60.44

Gene sequence:

>1011_bases
ATGTCTTCGATACTCGTCACCGGAGCAGCCGGGTTCATCGGTTTTCATCTTACGAAACGCCTTCTTGACCGGGGCGATCG
CGTGGTGGGGCTCGACAACCTCAACGACTATTATGACGTGAACCTGAAGCTGGACCGACTCCGCCAGTTGGAGGGGCGCG
AGGGATTCAGCTTTGTGCGGACCAGTCTGGCAGACCGGCCGGCCCTGGAGGATCTCTTTGCCGGCCAGCGTTTCGATGTG
GTGGTGAACCTGGCTGCCCAGGCCGGGGTCCGCTACTCCATCACCAACCCCCACGCTTACGTGGACAGTAATCTGGTCGG
CTTCATCAACATTCTGGAGGGGTGCCGGCATCACGGGGTGAAGCACTTGGTCTACGCATCGTCCAGCTCCGTCTATGGTG
CCAATACGGCAATGCCGTTTTCGATCCACCACAACGTGGATCATCCGGTTTCCCTGTATGCCGCCACCAAGAAGGCCAAC
GAGCTCATGGCCCACACCTATTCGAGCCTCTACGGGCTGCCCACCACGGGCCTGCGCTTCTTCACGGTCTACGGCCCCTG
GGGGCGCCCCGACATGGCGCTCTTCCTCTTTACCAAGGCAATCCTCGAAGGCCGGCCCATCGATGTCTATAATTTTGGCA
AAATGCAGCGTGATTTCACTTATGTAGACGACATTGTCGAGGGGGTGACGCGGGTCATGGACCGCACGCCGGAGCCCAAC
CCTGCCTGGAGCGGGGCCCGACCCGATCCCGGCACGAGCTACGCTCCCTATCGCATCTACAACATCGGCAACAACAACCC
GGTCGAGCTTCTCGCGTTCATTGAAGCCATCGAACAGAACCTGGGGATCACTGCGCAGAAGAATCTGCTTCCCCTGCAGG
CGGGTGACGTGCCCGCCACCTACGCCGACGTGGATGACCTGATGAACGACGTGGGGTTCAAGCCGGCCACTCCCATCGGG
GAGGGGATAGAGCGGTTCGTCGAGTGGTACCGGGGATACTACGGCGTCTGA

Upstream 100 bases:

>100_bases
GGGATCGCCCGGCTCCTTGCGACCCCCTTTCCATCAACCGGGTGATGTGCTACACTGCACCTCTTTCGCAATCACGCGTT
AAATCAAAAGGATAGCGTGC

Downstream 100 bases:

>100_bases
CGCCGTAAGAGGAGGACGTATGCCTGTTCTGGTCACCGGCGGTGCCGGCTATATTGGAAGCCATGTGGTCCGCCAGCTCT
CGGAGGCCGGCTATACGGTG

Product: capsular polysaccharide biosynthesis protein I

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSSILVTGAAGFIGFHLTKRLLDRGDRVVGLDNLNDYYDVNLKLDRLRQLEGREGFSFVRTSLADRPALEDLFAGQRFDV
VVNLAAQAGVRYSITNPHAYVDSNLVGFINILEGCRHHGVKHLVYASSSSVYGANTAMPFSIHHNVDHPVSLYAATKKAN
ELMAHTYSSLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIDVYNFGKMQRDFTYVDDIVEGVTRVMDRTPEPN
PAWSGARPDPGTSYAPYRIYNIGNNNPVELLAFIEAIEQNLGITAQKNLLPLQAGDVPATYADVDDLMNDVGFKPATPIG
EGIERFVEWYRGYYGV

Sequences:

>Translated_336_residues
MSSILVTGAAGFIGFHLTKRLLDRGDRVVGLDNLNDYYDVNLKLDRLRQLEGREGFSFVRTSLADRPALEDLFAGQRFDV
VVNLAAQAGVRYSITNPHAYVDSNLVGFINILEGCRHHGVKHLVYASSSSVYGANTAMPFSIHHNVDHPVSLYAATKKAN
ELMAHTYSSLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIDVYNFGKMQRDFTYVDDIVEGVTRVMDRTPEPN
PAWSGARPDPGTSYAPYRIYNIGNNNPVELLAFIEAIEQNLGITAQKNLLPLQAGDVPATYADVDDLMNDVGFKPATPIG
EGIERFVEWYRGYYGV
>Mature_335_residues
SSILVTGAAGFIGFHLTKRLLDRGDRVVGLDNLNDYYDVNLKLDRLRQLEGREGFSFVRTSLADRPALEDLFAGQRFDVV
VNLAAQAGVRYSITNPHAYVDSNLVGFINILEGCRHHGVKHLVYASSSSVYGANTAMPFSIHHNVDHPVSLYAATKKANE
LMAHTYSSLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIDVYNFGKMQRDFTYVDDIVEGVTRVMDRTPEPNP
AWSGARPDPGTSYAPYRIYNIGNNNPVELLAFIEAIEQNLGITAQKNLLPLQAGDVPATYADVDDLMNDVGFKPATPIGE
GIERFVEWYRGYYGV

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=24.9258160237389, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI56237023, Length=352, Percent_Identity=25.8522727272727, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI56118217, Length=352, Percent_Identity=25.8522727272727, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI189083684, Length=352, Percent_Identity=25.8522727272727, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=26.1127596439169, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=26.4788732394366, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994969, Length=354, Percent_Identity=27.683615819209, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786974, Length=345, Percent_Identity=23.4782608695652, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788589, Length=249, Percent_Identity=25.7028112449799, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=349, Percent_Identity=25.7879656160458, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71982038, Length=351, Percent_Identity=25.6410256410256, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17568069, Length=345, Percent_Identity=25.2173913043478, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17539532, Length=337, Percent_Identity=24.9258160237389, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI115532424, Length=342, Percent_Identity=22.8070175438596, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=350, Percent_Identity=25.1428571428571, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19923002, Length=349, Percent_Identity=26.647564469914, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356223, Length=339, Percent_Identity=25.9587020648968, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37356; Mature: 37224

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSILVTGAAGFIGFHLTKRLLDRGDRVVGLDNLNDYYDVNLKLDRLRQLEGREGFSFVR
CCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECEEHHHHHHHCCCCCHHHHH
TSLADRPALEDLFAGQRFDVVVNLAAQAGVRYSITNPHAYVDSNLVGFINILEGCRHHGV
HHHCCCCCHHHHHCCCCCHHEEEEHHHCCCEEEECCCCEEECCCHHHHHHHHHHHHHCCC
KHLVYASSSSVYGANTAMPFSIHHNVDHPVSLYAATKKANELMAHTYSSLYGLPTTGLRF
EEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEE
FTVYGPWGRPDMALFLFTKAILEGRPIDVYNFGKMQRDFTYVDDIVEGVTRVMDRTPEPN
EEEECCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PAWSGARPDPGTSYAPYRIYNIGNNNPVELLAFIEAIEQNLGITAQKNLLPLQAGDVPAT
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCCH
YADVDDLMNDVGFKPATPIGEGIERFVEWYRGYYGV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSILVTGAAGFIGFHLTKRLLDRGDRVVGLDNLNDYYDVNLKLDRLRQLEGREGFSFVR
CCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECEEHHHHHHHCCCCCHHHHH
TSLADRPALEDLFAGQRFDVVVNLAAQAGVRYSITNPHAYVDSNLVGFINILEGCRHHGV
HHHCCCCCHHHHHCCCCCHHEEEEHHHCCCEEEECCCCEEECCCHHHHHHHHHHHHHCCC
KHLVYASSSSVYGANTAMPFSIHHNVDHPVSLYAATKKANELMAHTYSSLYGLPTTGLRF
EEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEE
FTVYGPWGRPDMALFLFTKAILEGRPIDVYNFGKMQRDFTYVDDIVEGVTRVMDRTPEPN
EEEECCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PAWSGARPDPGTSYAPYRIYNIGNNNPVELLAFIEAIEQNLGITAQKNLLPLQAGDVPAT
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCCH
YADVDDLMNDVGFKPATPIGEGIERFVEWYRGYYGV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]