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The map label for this gene is miaB

Identifier: 39997296

GI number: 39997296

Start: 2410541

End: 2411881

Strand: Reverse

Name: miaB

Synonym: GSU2198

Alternate gene names: 39997296

Gene position: 2411881-2410541 (Counterclockwise)

Preceding gene: 39997300

Following gene: 39997295

Centisome position: 63.24

GC content: 60.48

Gene sequence:

>1341_bases
GTGACGGAAAACAAGTATCTGTATGTAGAAACATTCGGATGTCAGATGAATGTCAACGACTCCGAAAAGATCGCGACGCT
CCTGAAAGATGAGGGGTATCTCCCCACCGACGATCCGGAACGGGCCGACTTGGTCATTCTCAACACGTGCAGCGTGAGGG
CCAAGGCCGAGCAGAAAGTATACGGGCACCTTGGCCGGTTCAAGGGCGTCCGGAGCAGGAAGAAGGGATTCCTGCTCGGC
GTGGGGGGGTGTGTGGCACAGCAGGAGGGAGAGCGCCTGCTGCAGAAGGTTCCCTGGCTCGATCTGGTCTTCGGCACCCA
CAATCTCCACCTGCTGCCGGAGATTGTGCGGGCGGCCGAGCGGGGCGAGCGCCGGGCCGAGGTGGGGTTCATCGACAACG
AAACCCGGCTCGACCTGTTCCCCGAGACAGGTGGAGAGGGTGGTGTCACCCGCTTTGTGACGGTGATGCAGGGTTGTGAC
AATTTTTGTTCCTACTGCATTGTCCCCTATGTGAGGGGGCGCGAGATAAGTCGCCGTTCATCCGACATAATTGACGAGGT
GCGAAAGTCCGTTGCGGAGGGTGTCAAGGAAGTGACTCTTCTGGGGCAGAATGTCAACTCTTATGGTCTCAAGACCGAGG
GAGAGTTGTCGTTCGCCGGACTCATCCGTCGCATTGCCGAGGTGGAGGGGCTTGAACGGATACGTTTCACCACGTCACAC
CCGAAGGATATCTCGCCCGAGCTCATCGCCTGCTTCGCCGAGGTACCCAAGCTGTGCGGCCACATTCATCTGCCGGCCCA
GTCGGGGAGCGATGCGGTCCTTGCGCGCATGAACCGGGGATACACCCGGGCCGGGTACCTCGAAAAGGTGGAGGCCCTCA
GGGCAGCCCGGCCGGGTATCCAGTTCACGGGAGACATGATCGTGGGCTTCCCGGGTGAGACGGAAGAGGACTTCCAGGCA
ACCATCTCGCTCATGGAGGAAGTGCGCTACGCCGATCTCTTTTCCTTCATCTACTCGCCGCGCCCTGAAACGGCCGCGGC
CGGTATCCGGGATACGGTGAGCCGGAAGGAGAAGCAGTCGCGCCTCGACCGGCTCCAGACACTCCAGCAGCAGATGAAGC
GCGAGCGCAACATCAGCTTTGTCGGCACCCGCCAGCTTGTCCTGGTGGAGGGGGTGAGCAAGCGAGGCGATCAGCTCTAC
GGTCGCATCGACGGCAACCGGATCGTCAACTTCGCGGCCGACCAGTCGCTCATCGGAACCATGGCAGAGGTAACCATCAC
CCAGGACTATCAGAATTCGCTGCTGGGCGAAATCGTCACCGATAAAGGGGACGCACGGTAA

Upstream 100 bases:

>100_bases
ACGTAACGCGGCCATGACGGATGCCCGGTGGCGATAGCGGTTGACGTCCGAGCCCCCGATTGCTTACTATAGGCGGGAGT
TACCTGGAAAGAGGCACTAC

Downstream 100 bases:

>100_bases
TGCACATCGTTATGACCATCCACGGCTTTGCCCTTGACTCCATCGCCCAGATGCCGGTGGTGCTCCTGAAGGATGAACGC
GGCGAAGTAACGCTGCCCAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTENKYLYVETFGCQMNVNDSEKIATLLKDEGYLPTDDPERADLVILNTCSVRAKAEQKVYGHLGRFKGVRSRKKGFLLG
VGGCVAQQEGERLLQKVPWLDLVFGTHNLHLLPEIVRAAERGERRAEVGFIDNETRLDLFPETGGEGGVTRFVTVMQGCD
NFCSYCIVPYVRGREISRRSSDIIDEVRKSVAEGVKEVTLLGQNVNSYGLKTEGELSFAGLIRRIAEVEGLERIRFTTSH
PKDISPELIACFAEVPKLCGHIHLPAQSGSDAVLARMNRGYTRAGYLEKVEALRAARPGIQFTGDMIVGFPGETEEDFQA
TISLMEEVRYADLFSFIYSPRPETAAAGIRDTVSRKEKQSRLDRLQTLQQQMKRERNISFVGTRQLVLVEGVSKRGDQLY
GRIDGNRIVNFAADQSLIGTMAEVTITQDYQNSLLGEIVTDKGDAR

Sequences:

>Translated_446_residues
MTENKYLYVETFGCQMNVNDSEKIATLLKDEGYLPTDDPERADLVILNTCSVRAKAEQKVYGHLGRFKGVRSRKKGFLLG
VGGCVAQQEGERLLQKVPWLDLVFGTHNLHLLPEIVRAAERGERRAEVGFIDNETRLDLFPETGGEGGVTRFVTVMQGCD
NFCSYCIVPYVRGREISRRSSDIIDEVRKSVAEGVKEVTLLGQNVNSYGLKTEGELSFAGLIRRIAEVEGLERIRFTTSH
PKDISPELIACFAEVPKLCGHIHLPAQSGSDAVLARMNRGYTRAGYLEKVEALRAARPGIQFTGDMIVGFPGETEEDFQA
TISLMEEVRYADLFSFIYSPRPETAAAGIRDTVSRKEKQSRLDRLQTLQQQMKRERNISFVGTRQLVLVEGVSKRGDQLY
GRIDGNRIVNFAADQSLIGTMAEVTITQDYQNSLLGEIVTDKGDAR
>Mature_445_residues
TENKYLYVETFGCQMNVNDSEKIATLLKDEGYLPTDDPERADLVILNTCSVRAKAEQKVYGHLGRFKGVRSRKKGFLLGV
GGCVAQQEGERLLQKVPWLDLVFGTHNLHLLPEIVRAAERGERRAEVGFIDNETRLDLFPETGGEGGVTRFVTVMQGCDN
FCSYCIVPYVRGREISRRSSDIIDEVRKSVAEGVKEVTLLGQNVNSYGLKTEGELSFAGLIRRIAEVEGLERIRFTTSHP
KDISPELIACFAEVPKLCGHIHLPAQSGSDAVLARMNRGYTRAGYLEKVEALRAARPGIQFTGDMIVGFPGETEEDFQAT
ISLMEEVRYADLFSFIYSPRPETAAAGIRDTVSRKEKQSRLDRLQTLQQQMKRERNISFVGTRQLVLVEGVSKRGDQLYG
RIDGNRIVNFAADQSLIGTMAEVTITQDYQNSLLGEIVTDKGDAR

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=436, Percent_Identity=41.2844036697248, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI28872782, Length=436, Percent_Identity=41.2844036697248, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI93277076, Length=446, Percent_Identity=28.9237668161435, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=45.3514739229025, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787057, Length=398, Percent_Identity=28.1407035175879, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=459, Percent_Identity=38.1263616557734, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI71996771, Length=330, Percent_Identity=28.7878787878788, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21356207, Length=430, Percent_Identity=39.5348837209302, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI19922432, Length=451, Percent_Identity=29.490022172949, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_GEOSL (Q74B44)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953247.1
- ProteinModelPortal:   Q74B44
- SMR:   Q74B44
- GeneID:   2685659
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2198
- NMPDR:   fig|243231.1.peg.2185
- TIGR:   GSU2198
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14325
- BioCyc:   GSUL243231:GSU_2198-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49771; Mature: 49640

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENKYLYVETFGCQMNVNDSEKIATLLKDEGYLPTDDPERADLVILNTCSVRAKAEQKV
CCCCCEEEEEEECEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
YGHLGRFKGVRSRKKGFLLGVGGCVAQQEGERLLQKVPWLDLVFGTHNLHLLPEIVRAAE
HHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
RGERRAEVGFIDNETRLDLFPETGGEGGVTRFVTVMQGCDNFCSYCIVPYVRGREISRRS
CCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SDIIDEVRKSVAEGVKEVTLLGQNVNSYGLKTEGELSFAGLIRRIAEVEGLERIRFTTSH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCC
PKDISPELIACFAEVPKLCGHIHLPAQSGSDAVLARMNRGYTRAGYLEKVEALRAARPGI
CCCCCHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
QFTGDMIVGFPGETEEDFQATISLMEEVRYADLFSFIYSPRPETAAAGIRDTVSRKEKQS
EEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RLDRLQTLQQQMKRERNISFVGTRQLVLVEGVSKRGDQLYGRIDGNRIVNFAADQSLIGT
HHHHHHHHHHHHHHHCCCEEEECCEEEEEECCHHCCCCEEEEECCCEEEEEECCHHHHHH
MAEVTITQDYQNSLLGEIVTDKGDAR
HHHEEEEHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TENKYLYVETFGCQMNVNDSEKIATLLKDEGYLPTDDPERADLVILNTCSVRAKAEQKV
CCCCEEEEEEECEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
YGHLGRFKGVRSRKKGFLLGVGGCVAQQEGERLLQKVPWLDLVFGTHNLHLLPEIVRAAE
HHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
RGERRAEVGFIDNETRLDLFPETGGEGGVTRFVTVMQGCDNFCSYCIVPYVRGREISRRS
CCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SDIIDEVRKSVAEGVKEVTLLGQNVNSYGLKTEGELSFAGLIRRIAEVEGLERIRFTTSH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCC
PKDISPELIACFAEVPKLCGHIHLPAQSGSDAVLARMNRGYTRAGYLEKVEALRAARPGI
CCCCCHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
QFTGDMIVGFPGETEEDFQATISLMEEVRYADLFSFIYSPRPETAAAGIRDTVSRKEKQS
EEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RLDRLQTLQQQMKRERNISFVGTRQLVLVEGVSKRGDQLYGRIDGNRIVNFAADQSLIGT
HHHHHHHHHHHHHHHCCCEEEECCEEEEEECCHHCCCCEEEEECCCEEEEEECCHHHHHH
MAEVTITQDYQNSLLGEIVTDKGDAR
HHHEEEEHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA