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The map label for this gene is pflC [H]

Identifier: 39997200

GI number: 39997200

Start: 2314210

End: 2315106

Strand: Reverse

Name: pflC [H]

Synonym: GSU2102

Alternate gene names: 39997200

Gene position: 2315106-2314210 (Counterclockwise)

Preceding gene: 39997202

Following gene: 39997199

Centisome position: 60.7

GC content: 68.23

Gene sequence:

>897_bases
ATGATCGATCGGGGCATTGTCTTCAATATCCAGAAATATTCCATCTCCGATGGCCCCGGTATCCGCACGACCGTGTTTCT
GAAGGGGTGCCCGGCCAGTTGCTGGTGGTGCCACAACCCGGAAAGCCAGGCGCCGGCCCCGGAGATGACCCTGGTGGAAG
GGCGCTGCCTCTCCTGCGGCGAATGTGCCCGGGCCTGCCCGCACGGGACCGCGGGCCGCGGGCAGGGGCGCGAGGCGGCC
TGCCTGATCTGCGGCCGGTGCGCGGATGCCTGTCCCACCGGCGCGCGTCGCCTGGCCGGCACCGAGATGACCGTGGCCGA
CGTGCTTGCCGCGCTGCTGAAGGACCGGATCTTCTACGAGGAGTCGGGCGGCGGCGCAACCTTTTCCGGCGGCGAGCCCC
TGTGCCAGCCCGGTTTCCTCAAGGCGCTCCTGGCCGGCTGCCGGGAGCGGGGCATCCGGACGGCGGTTGATACCGGCGGA
TTTGCCCCGCGGGATCAGTTCCTGGAGGTCGCCACTGCCGCCGACCTGATCCTCTACGACCTGAAGCTGATGGATGCCGA
ACGGCACCGGGCCGTGGTTGGGCTGGACAACCGGATGGTACTGGACAACCTGGCGGCGCTCGGCGCGGTGCACGATACCA
TCTGGATCCGCATCCCGATCATTCCCGGCGTCAACGACGACCGCGCCAACCTGGAAGCCACCGCCGCCCTGGCGGCACGA
ACGCCGGGGGTGCGCCAGGTGAACCTGCTCCCCTATCACCGCACCTGGCACGGCAAACTCCGCCAGCTGGGCAGGGCAGG
GGCTGATGACGCAATCGCTACCCCCAGCAGGGAGCGGATGGAAGAACTGGTGCGGATATTCCGCGCCGCAGGACTGACCA
CCATCATAGGAGGATAG

Upstream 100 bases:

>100_bases
GGCTATCATTCAGCTTTCTGCGTCTCTCCCGGCCGTGTAATCCTTCAAGTTCGCCCCGAACCGGGGAGCGTAGCCGCATC
TGTCCGGCCAAGGATACTCG

Downstream 100 bases:

>100_bases
AGATATGACCGAGCGCACACGGGAACTGCGCCGCAGGAGCCTGGAGGCACGCCCCGCCATTTCCGCCGAACGGGCGCTGC
TGCTGACCGAATTTTACCGG

Product: pyruvate formate-lyase-activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 2; PFL-activating enzyme 2 [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MIDRGIVFNIQKYSISDGPGIRTTVFLKGCPASCWWCHNPESQAPAPEMTLVEGRCLSCGECARACPHGTAGRGQGREAA
CLICGRCADACPTGARRLAGTEMTVADVLAALLKDRIFYEESGGGATFSGGEPLCQPGFLKALLAGCRERGIRTAVDTGG
FAPRDQFLEVATAADLILYDLKLMDAERHRAVVGLDNRMVLDNLAALGAVHDTIWIRIPIIPGVNDDRANLEATAALAAR
TPGVRQVNLLPYHRTWHGKLRQLGRAGADDAIATPSRERMEELVRIFRAAGLTTIIGG

Sequences:

>Translated_298_residues
MIDRGIVFNIQKYSISDGPGIRTTVFLKGCPASCWWCHNPESQAPAPEMTLVEGRCLSCGECARACPHGTAGRGQGREAA
CLICGRCADACPTGARRLAGTEMTVADVLAALLKDRIFYEESGGGATFSGGEPLCQPGFLKALLAGCRERGIRTAVDTGG
FAPRDQFLEVATAADLILYDLKLMDAERHRAVVGLDNRMVLDNLAALGAVHDTIWIRIPIIPGVNDDRANLEATAALAAR
TPGVRQVNLLPYHRTWHGKLRQLGRAGADDAIATPSRERMEELVRIFRAAGLTTIIGG
>Mature_298_residues
MIDRGIVFNIQKYSISDGPGIRTTVFLKGCPASCWWCHNPESQAPAPEMTLVEGRCLSCGECARACPHGTAGRGQGREAA
CLICGRCADACPTGARRLAGTEMTVADVLAALLKDRIFYEESGGGATFSGGEPLCQPGFLKALLAGCRERGIRTAVDTGG
FAPRDQFLEVATAADLILYDLKLMDAERHRAVVGLDNRMVLDNLAALGAVHDTIWIRIPIIPGVNDDRANLEATAALAAR
TPGVRQVNLLPYHRTWHGKLRQLGRAGADDAIATPSRERMEELVRIFRAAGLTTIIGG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1790389, Length=297, Percent_Identity=36.3636363636364, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI226510931, Length=260, Percent_Identity=40, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1787130, Length=280, Percent_Identity=28.9285714285714, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790839, Length=300, Percent_Identity=28.6666666666667, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006638
- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 31909; Mature: 31909

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS01087 RADICAL_ACTIVATING ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRGIVFNIQKYSISDGPGIRTTVFLKGCPASCWWCHNPESQAPAPEMTLVEGRCLSCG
CCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCCEEEECCCCCCHH
ECARACPHGTAGRGQGREAACLICGRCADACPTGARRLAGTEMTVADVLAALLKDRIFYE
HHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHEEE
ESGGGATFSGGEPLCQPGFLKALLAGCRERGIRTAVDTGGFAPRDQFLEVATAADLILYD
ECCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
LKLMDAERHRAVVGLDNRMVLDNLAALGAVHDTIWIRIPIIPGVNDDRANLEATAALAAR
HHHHCCHHCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHC
TPGVRQVNLLPYHRTWHGKLRQLGRAGADDAIATPSRERMEELVRIFRAAGLTTIIGG
CCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCC
>Mature Secondary Structure
MIDRGIVFNIQKYSISDGPGIRTTVFLKGCPASCWWCHNPESQAPAPEMTLVEGRCLSCG
CCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCCEEEECCCCCCHH
ECARACPHGTAGRGQGREAACLICGRCADACPTGARRLAGTEMTVADVLAALLKDRIFYE
HHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHEEE
ESGGGATFSGGEPLCQPGFLKALLAGCRERGIRTAVDTGGFAPRDQFLEVATAADLILYD
ECCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
LKLMDAERHRAVVGLDNRMVLDNLAALGAVHDTIWIRIPIIPGVNDDRANLEATAALAAR
HHHHCCHHCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHC
TPGVRQVNLLPYHRTWHGKLRQLGRAGADDAIATPSRERMEELVRIFRAAGLTTIIGG
CCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]