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The map label for this gene is pflD [H]

Identifier: 39997199

GI number: 39997199

Start: 2311845

End: 2314205

Strand: Reverse

Name: pflD [H]

Synonym: GSU2101

Alternate gene names: 39997199

Gene position: 2314205-2311845 (Counterclockwise)

Preceding gene: 39997200

Following gene: 39997197

Centisome position: 60.67

GC content: 65.95

Gene sequence:

>2361_bases
ATGACCGAGCGCACACGGGAACTGCGCCGCAGGAGCCTGGAGGCACGCCCCGCCATTTCCGCCGAACGGGCGCTGCTGCT
GACCGAATTTTACCGGGAAAACGAGGGGAAATACTCGGTGCCGGTGATGCGCGCCCTGTCGTTCCTCCATATCTGCCGTC
ATAAGACCATCTGGATCGGGGAGGGCGAACTGGTCGTGGGCGAGCGGGGTCCTGAACCCAAGGCGGTCCCCACCTATCCC
GAACTGACCTGTCACAGCCTGGAAGACCTGCGTATTCTCGATTCGCGTCCCCTGACGAGCTATGCCGTTTCCGCCGAGTG
CCTCGCCGCCTACGAGGAAACGGTGATCCCCTACTGGCGGGGGCGCTCCCTGCGGGACAAGATGTTCGGCGAACTGGCCC
CGGAATGGCACGAGGCGTACGCCGCAGGCATCTTCACCGAATTCATGGAACAGCGCGCCCCGGGTCACACGGTCCTGGAC
GAGAAGCCCTTCCGGCGCGGCCTGCTGGATTTCAAGGCCGACATTGCCCGGGAGATCGGGCGGCTCGACTTCGTGCAGGA
CGGCGCGGCCTGGGAGAAGCGCGAGCAGCTCAGGGCCATGGCCATTTCCTGCGACGCGGCCATCCTCTTTGCCGAGCGGC
ATGCCGGCCTGGCCGACGAGATGGCGGCGCGGGAGACGGATCCGCACCGGAAGCGGGAACTTGAGCGCATCGCCGAGGTC
TGCCGCCATGTGCCGGCCCATGCGCCGCGCGACTTCCACGAAGCGCTCCAGGCCTACTGGTTCTGCCACCTGGCAATCAT
CACCGAGCTGAACGGGTGGGACGCCATGAGCCCCGGCCACCTGGACCAGCACCTGCTGCCGTTCTACGAGCAGGGTCTGG
CCCACGGCTCCCTGTCGCGGGATGCGGCGCGGGAGCTGCTGGAATGCTTCTTCGTCAAGTTCAACAACCACCCGGCCCCC
CCCAAGGTGGGAGTCACCGCCGCCGAGAGCGGCACCTACACCGATTTCGCCAACATCAACCTGGGCGGTCTGCTGCCCGA
CGGTTCCGACGGCTCCAACGAGGTGTCCCACCTCCTGCTCGACATCATCGACGAGATGCACCTGCTCCAGCCCAGCAGCA
ACATCCAGCTCTCCCGCAAGTCTCCCGACGCCTTCCTGAGCCATGCCCTGCGTGTCATCAGGAACGGGTACGGCTTTCCT
TCCATCTTCAACGCCGACAGCGTGGTGGAGGAGCAGTTGCGCCAGGGCAAGAGCCTGGTGGACGCCCGGGCCGGCGGCTG
CAGCGGCTGCGTCGAGGTGGGGGCGTTCGGCAAAGAGGCCTACATCCTGACCGGCTACTTCAACCTGGTGAAGCTCCTGG
AACTGGCCCTGCACAACGGCGTCGACCCTCGCACCGGCAGGCAGCTGGGCCCGGCCAGCGGCGATCCCTCCGGTTTTGCC
TCCTTCGACGACCTCTACGCCGCCTTCGAGGCGCAGCTGCGGCATTTCATCGAGATCAAGATGGCGGGCAACCAGCTGAT
CGAACAAATCTACTGCCGCCTCATGCCGGCGCCGTTCCTGTCGGTGCTGATCGACGACTGCATCGCAACCGGCAGGGACT
ACAACGCCGGCGGGGCGCGCTACAACAACACCTTCATCCAGGGCGTGGGGATCGGCAGCATCACCGACTCACTGTCCGCC
ATCAAGGCGCTGGTCTACGACTCCGGCCACATTGCGCTGCCGGACCTGGTGGCCGCACTTGACGCCGATTTTGCGGGGCA
GGAGCCGCTACGCCAGCGCCTGCTCAACAGGACCAGCAAATACGGCAACGACGACGACTACGCCGACGACCTTATGCGCC
GGGTCTTCGAGAGCTTCTTCAGGACCGTCGATGGCCGGCCCAACAGCAAGGGTGGGCAGTACCGGATCGAGATGCTCCCC
ACCACCTGTCACGTCTACTTCGGCTCGGTCACCGGCGCAACCCCGGACGGGCGCCGGGCCGGCACCCCGCTCTCGGAGGG
TATCTCCCCGGTCCAGGGGGCGGATCGCTCCGGGCCGACCGCGGTCATCAAATCGGCCGGCAAGATGGACCACATCAGAA
CCGGCGGCACCCTGCTCAACATGAAGTTTGCCCCCTCCCTCGTGGAAGGGGAGCAGGGGATCAGCACAATGGCCAGCCTC
GTGCGGAGCTATTTCCGCATGGACGGGCACCACGTCCAGTTCAACGTCGTGCGCGCCGACACGCTCCGCGCCGCCCAGGC
CGACCCCGATGCCCACCGCGACCTGATCGTGCGCGTGGCGGGCTACAGCGACTACTTCTGCGACCTGTCGCGGGAACTGC
AGGACGAGATCATCACGCGTACCGAGCATGATAGTCTCTGA

Upstream 100 bases:

>100_bases
GCTGATGACGCAATCGCTACCCCCAGCAGGGAGCGGATGGAAGAACTGGTGCGGATATTCCGCGCCGCAGGACTGACCAC
CATCATAGGAGGATAGAGAT

Downstream 100 bases:

>100_bases
TGTCGGCTGTTTGTTGGTTCTGGCGTGCCGTGGTTTCTGATGGGTAGGAGTTGTGGCGGTTGAGAGGATAGGGTGGGGAT
GGAGTCAACCGAAAGGTTAG

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 786; Mature: 785

Protein sequence:

>786_residues
MTERTRELRRRSLEARPAISAERALLLTEFYRENEGKYSVPVMRALSFLHICRHKTIWIGEGELVVGERGPEPKAVPTYP
ELTCHSLEDLRILDSRPLTSYAVSAECLAAYEETVIPYWRGRSLRDKMFGELAPEWHEAYAAGIFTEFMEQRAPGHTVLD
EKPFRRGLLDFKADIAREIGRLDFVQDGAAWEKREQLRAMAISCDAAILFAERHAGLADEMAARETDPHRKRELERIAEV
CRHVPAHAPRDFHEALQAYWFCHLAIITELNGWDAMSPGHLDQHLLPFYEQGLAHGSLSRDAARELLECFFVKFNNHPAP
PKVGVTAAESGTYTDFANINLGGLLPDGSDGSNEVSHLLLDIIDEMHLLQPSSNIQLSRKSPDAFLSHALRVIRNGYGFP
SIFNADSVVEEQLRQGKSLVDARAGGCSGCVEVGAFGKEAYILTGYFNLVKLLELALHNGVDPRTGRQLGPASGDPSGFA
SFDDLYAAFEAQLRHFIEIKMAGNQLIEQIYCRLMPAPFLSVLIDDCIATGRDYNAGGARYNNTFIQGVGIGSITDSLSA
IKALVYDSGHIALPDLVAALDADFAGQEPLRQRLLNRTSKYGNDDDYADDLMRRVFESFFRTVDGRPNSKGGQYRIEMLP
TTCHVYFGSVTGATPDGRRAGTPLSEGISPVQGADRSGPTAVIKSAGKMDHIRTGGTLLNMKFAPSLVEGEQGISTMASL
VRSYFRMDGHHVQFNVVRADTLRAAQADPDAHRDLIVRVAGYSDYFCDLSRELQDEIITRTEHDSL

Sequences:

>Translated_786_residues
MTERTRELRRRSLEARPAISAERALLLTEFYRENEGKYSVPVMRALSFLHICRHKTIWIGEGELVVGERGPEPKAVPTYP
ELTCHSLEDLRILDSRPLTSYAVSAECLAAYEETVIPYWRGRSLRDKMFGELAPEWHEAYAAGIFTEFMEQRAPGHTVLD
EKPFRRGLLDFKADIAREIGRLDFVQDGAAWEKREQLRAMAISCDAAILFAERHAGLADEMAARETDPHRKRELERIAEV
CRHVPAHAPRDFHEALQAYWFCHLAIITELNGWDAMSPGHLDQHLLPFYEQGLAHGSLSRDAARELLECFFVKFNNHPAP
PKVGVTAAESGTYTDFANINLGGLLPDGSDGSNEVSHLLLDIIDEMHLLQPSSNIQLSRKSPDAFLSHALRVIRNGYGFP
SIFNADSVVEEQLRQGKSLVDARAGGCSGCVEVGAFGKEAYILTGYFNLVKLLELALHNGVDPRTGRQLGPASGDPSGFA
SFDDLYAAFEAQLRHFIEIKMAGNQLIEQIYCRLMPAPFLSVLIDDCIATGRDYNAGGARYNNTFIQGVGIGSITDSLSA
IKALVYDSGHIALPDLVAALDADFAGQEPLRQRLLNRTSKYGNDDDYADDLMRRVFESFFRTVDGRPNSKGGQYRIEMLP
TTCHVYFGSVTGATPDGRRAGTPLSEGISPVQGADRSGPTAVIKSAGKMDHIRTGGTLLNMKFAPSLVEGEQGISTMASL
VRSYFRMDGHHVQFNVVRADTLRAAQADPDAHRDLIVRVAGYSDYFCDLSRELQDEIITRTEHDSL
>Mature_785_residues
TERTRELRRRSLEARPAISAERALLLTEFYRENEGKYSVPVMRALSFLHICRHKTIWIGEGELVVGERGPEPKAVPTYPE
LTCHSLEDLRILDSRPLTSYAVSAECLAAYEETVIPYWRGRSLRDKMFGELAPEWHEAYAAGIFTEFMEQRAPGHTVLDE
KPFRRGLLDFKADIAREIGRLDFVQDGAAWEKREQLRAMAISCDAAILFAERHAGLADEMAARETDPHRKRELERIAEVC
RHVPAHAPRDFHEALQAYWFCHLAIITELNGWDAMSPGHLDQHLLPFYEQGLAHGSLSRDAARELLECFFVKFNNHPAPP
KVGVTAAESGTYTDFANINLGGLLPDGSDGSNEVSHLLLDIIDEMHLLQPSSNIQLSRKSPDAFLSHALRVIRNGYGFPS
IFNADSVVEEQLRQGKSLVDARAGGCSGCVEVGAFGKEAYILTGYFNLVKLLELALHNGVDPRTGRQLGPASGDPSGFAS
FDDLYAAFEAQLRHFIEIKMAGNQLIEQIYCRLMPAPFLSVLIDDCIATGRDYNAGGARYNNTFIQGVGIGSITDSLSAI
KALVYDSGHIALPDLVAALDADFAGQEPLRQRLLNRTSKYGNDDDYADDLMRRVFESFFRTVDGRPNSKGGQYRIEMLPT
TCHVYFGSVTGATPDGRRAGTPLSEGISPVQGADRSGPTAVIKSAGKMDHIRTGGTLLNMKFAPSLVEGEQGISTMASLV
RSYFRMDGHHVQFNVVRADTLRAAQADPDAHRDLIVRVAGYSDYFCDLSRELQDEIITRTEHDSL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=793, Percent_Identity=35.3089533417402, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI1787044, Length=802, Percent_Identity=34.1645885286783, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI48994926, Length=548, Percent_Identity=26.8248175182482, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1787131, Length=555, Percent_Identity=24.8648648648649, Blast_Score=127, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 87209; Mature: 87078

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERTRELRRRSLEARPAISAERALLLTEFYRENEGKYSVPVMRALSFLHICRHKTIWIG
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEC
EGELVVGERGPEPKAVPTYPELTCHSLEDLRILDSRPLTSYAVSAECLAAYEETVIPYWR
CCCEEECCCCCCCCCCCCCCCCHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHC
GRSLRDKMFGELAPEWHEAYAAGIFTEFMEQRAPGHTVLDEKPFRRGLLDFKADIAREIG
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RLDFVQDGAAWEKREQLRAMAISCDAAILFAERHAGLADEMAARETDPHRKRELERIAEV
CCCHHCCCCHHHHHHHHHHHHHCCCHHEEEHHHHCCCHHHHHHCCCCHHHHHHHHHHHHH
CRHVPAHAPRDFHEALQAYWFCHLAIITELNGWDAMSPGHLDQHLLPFYEQGLAHGSLSR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCH
DAARELLECFFVKFNNHPAPPKVGVTAAESGTYTDFANINLGGLLPDGSDGSNEVSHLLL
HHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHH
DIIDEMHLLQPSSNIQLSRKSPDAFLSHALRVIRNGYGFPSIFNADSVVEEQLRQGKSLV
HHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHH
DARAGGCSGCVEVGAFGKEAYILTGYFNLVKLLELALHNGVDPRTGRQLGPASGDPSGFA
HHCCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
SFDDLYAAFEAQLRHFIEIKMAGNQLIEQIYCRLMPAPFLSVLIDDCIATGRDYNAGGAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCE
YNNTFIQGVGIGSITDSLSAIKALVYDSGHIALPDLVAALDADFAGQEPLRQRLLNRTSK
ECCEEEEECCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHC
YGNDDDYADDLMRRVFESFFRTVDGRPNSKGGQYRIEMLPTTCHVYFGSVTGATPDGRRA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCC
GTPLSEGISPVQGADRSGPTAVIKSAGKMDHIRTGGTLLNMKFAPSLVEGEQGISTMASL
CCCHHHCCCCCCCCCCCCCHHHHHCCCCCCCEECCCEEEEEECCCCHHCCCCCHHHHHHH
VRSYFRMDGHHVQFNVVRADTLRAAQADPDAHRDLIVRVAGYSDYFCDLSRELQDEIITR
HHHHHHCCCCEEEEEEEEHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHC
TEHDSL
CCCCCC
>Mature Secondary Structure 
TERTRELRRRSLEARPAISAERALLLTEFYRENEGKYSVPVMRALSFLHICRHKTIWIG
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEC
EGELVVGERGPEPKAVPTYPELTCHSLEDLRILDSRPLTSYAVSAECLAAYEETVIPYWR
CCCEEECCCCCCCCCCCCCCCCHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHC
GRSLRDKMFGELAPEWHEAYAAGIFTEFMEQRAPGHTVLDEKPFRRGLLDFKADIAREIG
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RLDFVQDGAAWEKREQLRAMAISCDAAILFAERHAGLADEMAARETDPHRKRELERIAEV
CCCHHCCCCHHHHHHHHHHHHHCCCHHEEEHHHHCCCHHHHHHCCCCHHHHHHHHHHHHH
CRHVPAHAPRDFHEALQAYWFCHLAIITELNGWDAMSPGHLDQHLLPFYEQGLAHGSLSR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCH
DAARELLECFFVKFNNHPAPPKVGVTAAESGTYTDFANINLGGLLPDGSDGSNEVSHLLL
HHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHH
DIIDEMHLLQPSSNIQLSRKSPDAFLSHALRVIRNGYGFPSIFNADSVVEEQLRQGKSLV
HHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHH
DARAGGCSGCVEVGAFGKEAYILTGYFNLVKLLELALHNGVDPRTGRQLGPASGDPSGFA
HHCCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
SFDDLYAAFEAQLRHFIEIKMAGNQLIEQIYCRLMPAPFLSVLIDDCIATGRDYNAGGAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCE
YNNTFIQGVGIGSITDSLSAIKALVYDSGHIALPDLVAALDADFAGQEPLRQRLLNRTSK
ECCEEEEECCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHC
YGNDDDYADDLMRRVFESFFRTVDGRPNSKGGQYRIEMLPTTCHVYFGSVTGATPDGRRA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCC
GTPLSEGISPVQGADRSGPTAVIKSAGKMDHIRTGGTLLNMKFAPSLVEGEQGISTMASL
CCCHHHCCCCCCCCCCCCCHHHHHCCCCCCCEECCCEEEEEECCCCHHCCCCCHHHHHHH
VRSYFRMDGHHVQFNVVRADTLRAAQADPDAHRDLIVRVAGYSDYFCDLSRELQDEIITR
HHHHHHCCCCEEEEEEEEHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHC
TEHDSL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]