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The map label for this gene is cooF [H]

Identifier: 39997194

GI number: 39997194

Start: 2304084

End: 2304677

Strand: Reverse

Name: cooF [H]

Synonym: GSU2096

Alternate gene names: 39997194

Gene position: 2304677-2304084 (Counterclockwise)

Preceding gene: 39997195

Following gene: 39997193

Centisome position: 60.42

GC content: 68.69

Gene sequence:

>594_bases
ATGAAACGGATATTCGTCGATTACAAAAAGTGCCTCGCCTGCAAGGCCTGCGAAACCGCGTGCGCCGTGCGGCACCATCC
CGGCGGCTCCCTCCTGGCCGCCCTCGGCGACGACCGGACCGAAGTGAATGTCCGGGTGCTCGGCATCGACGAGGAGGCAT
TTCCGCTCTCCTGCCGGCACTGCGACCCGGCCGAGTGCCTCGATGCCTGCCCCTCGGGCGCCCTGGGCCGGGACCCGGAA
AACGGCGCGGTCATCCTGGACCCGGCGCGCTGCAAGGCCTGCGCGATGTGCGCCATGGTCTGCCCCTTCGATGCCATTTC
CTTCAAGGCCACCCACCGCTCCGACTACGGCCGGGACGTGGCCCACAAGTGCGACCTGTGCACCCCACGGGTCAGGGAAG
GGGGGACTCCAGCCTGTGTCGAGGCCTGCCATTCCGGCGCCCTCGTCTTTGCCGAGTACGGGGCCGTCCGCGCCGAACGG
GCCGCCAAAAGCTTGCGGCACTACCTCCTTGGCAGGGAAGGACTGCCGCCCGGCCTCGAACTGCTGCGGGAGATGCGCCG
GAAAGAGTTTGCCCGGCGCCGCGGAGGTGAGTGA

Upstream 100 bases:

>100_bases
CGCCTGGCATCAGTGCTCTGCTCCCCGCCGTGCCCCTGCGCCTCTCCCCGCGACCATGCCCGCCACCATCACCATGCCCA
TTGACGAGGGAGACCCTGCA

Downstream 100 bases:

>100_bases
TGAAGGTGGTAACCGTGGGGACGGGAATGGCGGCCGCCGAGTTCGTCGAGCGGCTCAGGCTCGACGGATTCGCGGGCGAG
ATCGTCATGTGCGGTGACGA

Product: iron-sulfur cluster-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MKRIFVDYKKCLACKACETACAVRHHPGGSLLAALGDDRTEVNVRVLGIDEEAFPLSCRHCDPAECLDACPSGALGRDPE
NGAVILDPARCKACAMCAMVCPFDAISFKATHRSDYGRDVAHKCDLCTPRVREGGTPACVEACHSGALVFAEYGAVRAER
AAKSLRHYLLGREGLPPGLELLREMRRKEFARRRGGE

Sequences:

>Translated_197_residues
MKRIFVDYKKCLACKACETACAVRHHPGGSLLAALGDDRTEVNVRVLGIDEEAFPLSCRHCDPAECLDACPSGALGRDPE
NGAVILDPARCKACAMCAMVCPFDAISFKATHRSDYGRDVAHKCDLCTPRVREGGTPACVEACHSGALVFAEYGAVRAER
AAKSLRHYLLGREGLPPGLELLREMRRKEFARRRGGE
>Mature_197_residues
MKRIFVDYKKCLACKACETACAVRHHPGGSLLAALGDDRTEVNVRVLGIDEEAFPLSCRHCDPAECLDACPSGALGRDPE
NGAVILDPARCKACAMCAMVCPFDAISFKATHRSDYGRDVAHKCDLCTPRVREGGTPACVEACHSGALVFAEYGAVRAER
AAKSLRHYLLGREGLPPGLELLREMRRKEFARRRGGE

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH). CooF is required in stoichiometric amounts in vitro for anchoring CODH to the membrane as well as

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI87082180, Length=163, Percent_Identity=34.3558282208589, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI2367245, Length=157, Percent_Identity=36.3057324840764, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1787872, Length=165, Percent_Identity=32.7272727272727, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI2367345, Length=148, Percent_Identity=35.1351351351351, Blast_Score=78, Evalue=3e-16,
Organism=Escherichia coli, GI1787122, Length=164, Percent_Identity=32.9268292682927, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1789370, Length=222, Percent_Identity=25.6756756756757, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI87082179, Length=164, Percent_Identity=35.3658536585366, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI226510944, Length=177, Percent_Identity=31.0734463276836, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1789067, Length=176, Percent_Identity=32.3863636363636, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788811, Length=164, Percent_Identity=29.8780487804878, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1789079, Length=173, Percent_Identity=30.0578034682081, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1790326, Length=178, Percent_Identity=27.5280898876405, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787740, Length=118, Percent_Identity=32.2033898305085, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787749, Length=161, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787478, Length=116, Percent_Identity=32.7586206896552, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 21420; Mature: 21420

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
10.2 %Cys+Met (Translated Protein)
8.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
10.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIFVDYKKCLACKACETACAVRHHPGGSLLAALGDDRTEVNVRVLGIDEEAFPLSCRH
CCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCC
CDPAECLDACPSGALGRDPENGAVILDPARCKACAMCAMVCPFDAISFKATHRSDYGRDV
CCHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCHHEEEECCCCCCCHHH
AHKCDLCTPRVREGGTPACVEACHSGALVFAEYGAVRAERAAKSLRHYLLGREGLPPGLE
HHHCCCCCCHHHCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LLREMRRKEFARRRGGE
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRIFVDYKKCLACKACETACAVRHHPGGSLLAALGDDRTEVNVRVLGIDEEAFPLSCRH
CCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCC
CDPAECLDACPSGALGRDPENGAVILDPARCKACAMCAMVCPFDAISFKATHRSDYGRDV
CCHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCHHEEEECCCCCCCHHH
AHKCDLCTPRVREGGTPACVEACHSGALVFAEYGAVRAERAAKSLRHYLLGREGLPPGLE
HHHCCCCCCHHHCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LLREMRRKEFARRRGGE
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1644755 [H]