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The map label for this gene is gph [H]

Identifier: 39997167

GI number: 39997167

Start: 2271279

End: 2271917

Strand: Reverse

Name: gph [H]

Synonym: GSU2069

Alternate gene names: 39997167

Gene position: 2271917-2271279 (Counterclockwise)

Preceding gene: 39997168

Following gene: 39997163

Centisome position: 59.57

GC content: 63.54

Gene sequence:

>639_bases
ATGGCGGTGAGCAGTAACGGCGGGGCCATCAAGGCGGTAATCTACGATTGTGACGGGGTCATGTTCGACTCATTCGAGGC
GAACCTCGCGTTTTACCAGCGGATCATGGAGATGATGGGCCGCCCCCGGCTCAGCAGGGATAACGAGGAGCAGATGCGCA
TCCTCCACACGTACGCCAATCGGGAGGTGCTGGCCCATTTCTTCCCGTCACCCGGCGACTGGGAGGAGGCGGTCCGGTGT
GCCGGCGCCATCGACTACCGGGAACTGGTCCCGCTCATGATCATGGAGGAGGGATTCCGCGAGGCCCTCGACACCCTGAA
GGGGAGGGTGGGGCTCGGTGTCTGCACCAACCGGTCGACCTCCATGGACATGGTGCTCCGGTTGTTCAGTCTCGACTCCT
ATTTCAGCATTGTCATGACCGCGTCACGGGTGACGAACCCAAAACCCCACCCGGAACCCCTGCTGAAGGTGCTCGAACAC
TTCGGCATCGGCCCACGGGAGGCCCTGTTCGTGGGTGATTCCGAGGTGGACCGGTTGTCGGCCGAGGCTGCCGGCGTCCC
CTTCGTGGCCTACAAGGCCCCGCTCCCCGCCGCGTACCGGATGGAGCACCACCGCGAGATCATAGACCTTCTCGGGTGA

Upstream 100 bases:

>100_bases
AGCAGCAGGGGAAACGTGGTACCGGCCTGCAAGGAATGCAATAACCGGAAGAAATACCTCCTCCCCCTGGAGTGGGAAGA
GTATCTCGAAAAGGCGAGGA

Downstream 100 bases:

>100_bases
CCGGGCCGCCCGGCACCGCCCCCCCTCATACGCCCAGCACCTCGGCCAAGCGCAGCACCTCGTCCTGAGGACGCTTCTCA
CCCTTGATGACATCCTCCAG

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MAVSSNGGAIKAVIYDCDGVMFDSFEANLAFYQRIMEMMGRPRLSRDNEEQMRILHTYANREVLAHFFPSPGDWEEAVRC
AGAIDYRELVPLMIMEEGFREALDTLKGRVGLGVCTNRSTSMDMVLRLFSLDSYFSIVMTASRVTNPKPHPEPLLKVLEH
FGIGPREALFVGDSEVDRLSAEAAGVPFVAYKAPLPAAYRMEHHREIIDLLG

Sequences:

>Translated_212_residues
MAVSSNGGAIKAVIYDCDGVMFDSFEANLAFYQRIMEMMGRPRLSRDNEEQMRILHTYANREVLAHFFPSPGDWEEAVRC
AGAIDYRELVPLMIMEEGFREALDTLKGRVGLGVCTNRSTSMDMVLRLFSLDSYFSIVMTASRVTNPKPHPEPLLKVLEH
FGIGPREALFVGDSEVDRLSAEAAGVPFVAYKAPLPAAYRMEHHREIIDLLG
>Mature_211_residues
AVSSNGGAIKAVIYDCDGVMFDSFEANLAFYQRIMEMMGRPRLSRDNEEQMRILHTYANREVLAHFFPSPGDWEEAVRCA
GAIDYRELVPLMIMEEGFREALDTLKGRVGLGVCTNRSTSMDMVLRLFSLDSYFSIVMTASRVTNPKPHPEPLLKVLEHF
GIGPREALFVGDSEVDRLSAEAAGVPFVAYKAPLPAAYRMEHHREIIDLLG

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=163, Percent_Identity=33.1288343558282, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23777; Mature: 23646

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSSNGGAIKAVIYDCDGVMFDSFEANLAFYQRIMEMMGRPRLSRDNEEQMRILHTYAN
CCCCCCCCEEEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
REVLAHFFPSPGDWEEAVRCAGAIDYRELVPLMIMEEGFREALDTLKGRVGLGVCTNRST
HHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
SMDMVLRLFSLDSYFSIVMTASRVTNPKPHPEPLLKVLEHFGIGPREALFVGDSEVDRLS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHH
AEAAGVPFVAYKAPLPAAYRMEHHREIIDLLG
HHHCCCCEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVSSNGGAIKAVIYDCDGVMFDSFEANLAFYQRIMEMMGRPRLSRDNEEQMRILHTYAN
CCCCCCCEEEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
REVLAHFFPSPGDWEEAVRCAGAIDYRELVPLMIMEEGFREALDTLKGRVGLGVCTNRST
HHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
SMDMVLRLFSLDSYFSIVMTASRVTNPKPHPEPLLKVLEHFGIGPREALFVGDSEVDRLS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHH
AEAAGVPFVAYKAPLPAAYRMEHHREIIDLLG
HHHCCCCEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]