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The map label for this gene is ppnK

Identifier: 39997163

GI number: 39997163

Start: 2265431

End: 2266285

Strand: Reverse

Name: ppnK

Synonym: GSU2065

Alternate gene names: 39997163

Gene position: 2266285-2265431 (Counterclockwise)

Preceding gene: 39997167

Following gene: 39997162

Centisome position: 59.42

GC content: 62.92

Gene sequence:

>855_bases
ATGAAAAAAATCGCTATCTTCGCCAAGGTTCACGATCCCCGCTGCCAGGGCGTGGCGAGCGAGCTGATCGCCTGGCTGGA
GGCGCGGGGACTGATTCCGCTGGTGGAGGCCCATTTGGCGCGCCACTTGGGGGGGCGGCAGGGCATCGTCCCCGAGGACA
TTCCCGTGCTGGCCGATATGGCGGTGGTCCTCGGCGGCGACGGGACCCTCATTTCCGCGGCGCGGCTCATCGGGTCCCGC
CAGATTCCCATTCTCGGCGTCAACCTGGGCAGTCTCGGCTTTCTGACCGAGATCACCCTGGACGAGCTCTATCCGGTGCT
GGAATCCTGTCTCTCGGGCGATTTTCAGGTGACCGAGCGGATGATGCTTACCGTGTCCGTAGAGCGTAACGGCGAGGAGA
TCTGTTCCCACCGGGTTCTGAACGATGTGGTCATCAACAAGGGAGCCCTTGCCAGGATCATCGACATGGAGACGGAGGTG
AGCGGCATCCGGCTCACCACCTACAAGGCCGACGGCCTCATTATTTCAACTCCCACGGGTTCCACCGGCTACAGCCTTTC
GGCCAACGGACCCATCGTTCATCCGTCACTGGAGTGCATCACGATCACCCCCATCTGTCCCCACACCCTCACCAACCGTC
CCATCGTGCTCGAGTCGAGTTCAGGAGTAACCGTCTGGCTCCGCTCCAAGGACGAGGATGTCTACCTGACGCTGGACGGC
CAGGTGGGGATGGAGCTGAAATGCGGCGACGCGGTGCATGTGCGCCGGGCGGCCCACAGGACCCGGCTCGTCATGTCCCG
CAGCCGCAACTACTTCGAGGTACTCCGGACCAAGCTCAAGTGGGGGGAGCGGTAG

Upstream 100 bases:

>100_bases
AGGCAGGTCCAGGCGCTAAACGGCCGAATTTCCATTGGACATGCCACCTGATCTGTGTTAGTAAAATTCAAGTTTATACC
ATATCCGGAAGGCCGCACGA

Downstream 100 bases:

>100_bases
TGCTGACCGATCTCTCCATCAGGAACCTCGCCATCATCGATTCCCTTCATGTTTCCTTCGACCGGGGGCTCACCGTCCTT
ACGGGCGAGACCGGCGCGGG

Product: ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKKIAIFAKVHDPRCQGVASELIAWLEARGLIPLVEAHLARHLGGRQGIVPEDIPVLADMAVVLGGDGTLISAARLIGSR
QIPILGVNLGSLGFLTEITLDELYPVLESCLSGDFQVTERMMLTVSVERNGEEICSHRVLNDVVINKGALARIIDMETEV
SGIRLTTYKADGLIISTPTGSTGYSLSANGPIVHPSLECITITPICPHTLTNRPIVLESSSGVTVWLRSKDEDVYLTLDG
QVGMELKCGDAVHVRRAAHRTRLVMSRSRNYFEVLRTKLKWGER

Sequences:

>Translated_284_residues
MKKIAIFAKVHDPRCQGVASELIAWLEARGLIPLVEAHLARHLGGRQGIVPEDIPVLADMAVVLGGDGTLISAARLIGSR
QIPILGVNLGSLGFLTEITLDELYPVLESCLSGDFQVTERMMLTVSVERNGEEICSHRVLNDVVINKGALARIIDMETEV
SGIRLTTYKADGLIISTPTGSTGYSLSANGPIVHPSLECITITPICPHTLTNRPIVLESSSGVTVWLRSKDEDVYLTLDG
QVGMELKCGDAVHVRRAAHRTRLVMSRSRNYFEVLRTKLKWGER
>Mature_284_residues
MKKIAIFAKVHDPRCQGVASELIAWLEARGLIPLVEAHLARHLGGRQGIVPEDIPVLADMAVVLGGDGTLISAARLIGSR
QIPILGVNLGSLGFLTEITLDELYPVLESCLSGDFQVTERMMLTVSVERNGEEICSHRVLNDVVINKGALARIIDMETEV
SGIRLTTYKADGLIISTPTGSTGYSLSANGPIVHPSLECITITPICPHTLTNRPIVLESSSGVTVWLRSKDEDVYLTLDG
QVGMELKCGDAVHVRRAAHRTRLVMSRSRNYFEVLRTKLKWGER

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=262, Percent_Identity=29.7709923664122, Blast_Score=118, Evalue=5e-27,
Organism=Escherichia coli, GI1788968, Length=290, Percent_Identity=36.2068965517241, Blast_Score=186, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6320794, Length=244, Percent_Identity=34.0163934426229, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322509, Length=245, Percent_Identity=31.8367346938775, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325068, Length=212, Percent_Identity=34.4339622641509, Blast_Score=125, Evalue=8e-30,
Organism=Drosophila melanogaster, GI28573828, Length=254, Percent_Identity=32.2834645669291, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI28573832, Length=254, Percent_Identity=32.2834645669291, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28573826, Length=254, Percent_Identity=32.2834645669291, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28573830, Length=254, Percent_Identity=32.2834645669291, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161077047, Length=254, Percent_Identity=32.2834645669291, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281363321, Length=296, Percent_Identity=30.0675675675676, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20129957, Length=296, Percent_Identity=30.0675675675676, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281363323, Length=296, Percent_Identity=30.0675675675676, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653424, Length=296, Percent_Identity=30.0675675675676, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24653422, Length=296, Percent_Identity=30.0675675675676, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_GEOSL (Q74BH6)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953114.1
- ProteinModelPortal:   Q74BH6
- SMR:   Q74BH6
- GeneID:   2686010
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2065
- NMPDR:   fig|243231.1.peg.2052
- TIGR:   GSU2065
- HOGENOM:   HBG713904
- OMA:   KLHWAGS
- ProtClustDB:   CLSK828714
- BioCyc:   GSUL243231:GSU_2065-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 30999; Mature: 30999

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAIFAKVHDPRCQGVASELIAWLEARGLIPLVEAHLARHLGGRQGIVPEDIPVLADM
CCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHEE
AVVLGGDGTLISAARLIGSRQIPILGVNLGSLGFLTEITLDELYPVLESCLSGDFQVTER
EEEECCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
MMLTVSVERNGEEICSHRVLNDVVINKGALARIIDMETEVSGIRLTTYKADGLIISTPTG
EEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCEEEECCCC
STGYSLSANGPIVHPSLECITITPICPHTLTNRPIVLESSSGVTVWLRSKDEDVYLTLDG
CCCEEEECCCCEECCCCEEEEECCCCCCCCCCCCEEEECCCCEEEEEECCCCEEEEEECC
QVGMELKCGDAVHVRRAAHRTRLVMSRSRNYFEVLRTKLKWGER
CCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKIAIFAKVHDPRCQGVASELIAWLEARGLIPLVEAHLARHLGGRQGIVPEDIPVLADM
CCEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHEE
AVVLGGDGTLISAARLIGSRQIPILGVNLGSLGFLTEITLDELYPVLESCLSGDFQVTER
EEEECCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
MMLTVSVERNGEEICSHRVLNDVVINKGALARIIDMETEVSGIRLTTYKADGLIISTPTG
EEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCEEEECCCC
STGYSLSANGPIVHPSLECITITPICPHTLTNRPIVLESSSGVTVWLRSKDEDVYLTLDG
CCCEEEECCCCEECCCCEEEEECCCCCCCCCCCCEEEECCCCEEEEEECCCCEEEEEECC
QVGMELKCGDAVHVRRAAHRTRLVMSRSRNYFEVLRTKLKWGER
CCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA