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The map label for this gene is ntrB [H]

Identifier: 39997088

GI number: 39997088

Start: 2183375

End: 2184382

Strand: Reverse

Name: ntrB [H]

Synonym: GSU1990

Alternate gene names: 39997088

Gene position: 2184382-2183375 (Counterclockwise)

Preceding gene: 39997090

Following gene: 39997087

Centisome position: 57.27

GC content: 55.85

Gene sequence:

>1008_bases
ATGGTTATGAGACGATACTCATTTATGGTCGACGATCAACTGCGGCTCGTGGCACGAGACGATCTGCACCGTTCGGTGGG
GAACGATCCCTTGTTGGGTATTCCCTATTACGAATGCTATCCCCGGCTTTGGAACGGCAACGTTGATGCCGTTCGTACCG
TCGTGGATCAGGGAGAGCCTCTGGTTCTCAACGGTTATCGCCACTACTGCTTTTATGGCGAGTTCAGTGCGGCAGACATT
GAAATTCGCCCCGTTTTCGATGGTGCCTCCCGATGCACCGGCGCAATGGTGCAGGTTTCCGTGCTCCCTGGATGTACGGT
CTATGGTGAAATGGACAGCGCCCGGGCGCTGATAGACATCGGCAAGATGTCGGTGACGCTTGCCCATGGCGTACGCAACC
CGCTCAATGCCATCAAGGGCGCCGTGGTCTATCTCAAGGACAAGTACTGCACTGACGCAACCTTTGCCGAATTCGCCGAT
ATTATCGACGAAGAGATTTGCAAGCTCGACGGGTTCATTACCGAGTTCCTCGGTACCTCGCACCTGGAACCGGTCAGAGA
GGAAATCCAGCTCAACGATCTCCTTGAGCGCGTCGTAAAATTCGTTTCCCTGCAGGCAGATGCGAATCATGTGCGATTTG
ACGTGGAGTACGGTGAGTTGCCGCTCGTCATGCTCGATTCGTTCAATTTCGGTCACGCCATACTCAATATCGTCAACAAC
GCTCTCGGGGCCATGGAGGCGGGAGGCTCCCTTACCATGCGAACCAGCACATTGCTGGAAGGTGGAGTGGAGATGATTGT
GGTCGAGGTGGCCGATACGGGGTCGGGCCTTCGGTCGGGGGGCAAGGGGATGCTTGGCAGTTTTCCCGGAGCCGGCAGCA
GGAAGCAGGGGAAAGGGTACGGCCTGTTCATCACCCGTGAGATTGTACGCCACCACCAGGGGAAGATTGAAATAACCGGG
AATCATGAAGGCGGAACCACCGTCAAGATCATGTTGCCGGCGGTATAG

Upstream 100 bases:

>100_bases
AGCCTATCACGGGCAGATCGTAAAGGACCGATGTAAAACAGGGAGGTTGAACGGCATACGCGAGAGTCCGCTTCGGGCAA
ATCGCTCTTTGCCGGAATGG

Downstream 100 bases:

>100_bases
CCGGATCGGTGCGGTCCAGGAGGAGCGAAGTGCAGGGTCTTGTTCTTATCGTCGACGATGAGCCGAATGCGGCCAGGGTT
CTTTCGGCAATCCTGGCCGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MVMRRYSFMVDDQLRLVARDDLHRSVGNDPLLGIPYYECYPRLWNGNVDAVRTVVDQGEPLVLNGYRHYCFYGEFSAADI
EIRPVFDGASRCTGAMVQVSVLPGCTVYGEMDSARALIDIGKMSVTLAHGVRNPLNAIKGAVVYLKDKYCTDATFAEFAD
IIDEEICKLDGFITEFLGTSHLEPVREEIQLNDLLERVVKFVSLQADANHVRFDVEYGELPLVMLDSFNFGHAILNIVNN
ALGAMEAGGSLTMRTSTLLEGGVEMIVVEVADTGSGLRSGGKGMLGSFPGAGSRKQGKGYGLFITREIVRHHQGKIEITG
NHEGGTTVKIMLPAV

Sequences:

>Translated_335_residues
MVMRRYSFMVDDQLRLVARDDLHRSVGNDPLLGIPYYECYPRLWNGNVDAVRTVVDQGEPLVLNGYRHYCFYGEFSAADI
EIRPVFDGASRCTGAMVQVSVLPGCTVYGEMDSARALIDIGKMSVTLAHGVRNPLNAIKGAVVYLKDKYCTDATFAEFAD
IIDEEICKLDGFITEFLGTSHLEPVREEIQLNDLLERVVKFVSLQADANHVRFDVEYGELPLVMLDSFNFGHAILNIVNN
ALGAMEAGGSLTMRTSTLLEGGVEMIVVEVADTGSGLRSGGKGMLGSFPGAGSRKQGKGYGLFITREIVRHHQGKIEITG
NHEGGTTVKIMLPAV
>Mature_335_residues
MVMRRYSFMVDDQLRLVARDDLHRSVGNDPLLGIPYYECYPRLWNGNVDAVRTVVDQGEPLVLNGYRHYCFYGEFSAADI
EIRPVFDGASRCTGAMVQVSVLPGCTVYGEMDSARALIDIGKMSVTLAHGVRNPLNAIKGAVVYLKDKYCTDATFAEFAD
IIDEEICKLDGFITEFLGTSHLEPVREEIQLNDLLERVVKFVSLQADANHVRFDVEYGELPLVMLDSFNFGHAILNIVNN
ALGAMEAGGSLTMRTSTLLEGGVEMIVVEVADTGSGLRSGGKGMLGSFPGAGSRKQGKGYGLFITREIVRHHQGKIEITG
NHEGGTTVKIMLPAV

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=310, Percent_Identity=27.4193548387097, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790436, Length=228, Percent_Identity=25, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788549, Length=226, Percent_Identity=27.4336283185841, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 36690; Mature: 36690

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMRRYSFMVDDQLRLVARDDLHRSVGNDPLLGIPYYECYPRLWNGNVDAVRTVVDQGEP
CCEEEEEEEECCCHHEEEHHHHHHHCCCCCEECCCHHHHHHHHCCCCHHHHHHHHHCCCC
LVLNGYRHYCFYGEFSAADIEIRPVFDGASRCTGAMVQVSVLPGCTVYGEMDSARALIDI
EEEECCEEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEEECCCCCCCEEEEC
GKMSVTLAHGVRNPLNAIKGAVVYLKDKYCTDATFAEFADIIDEEICKLDGFITEFLGTS
CCCEEEEHHHCCCHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
HLEPVREEIQLNDLLERVVKFVSLQADANHVRFDVEYGELPLVMLDSFNFGHAILNIVNN
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHH
ALGAMEAGGSLTMRTSTLLEGGVEMIVVEVADTGSGLRSGGKGMLGSFPGAGSRKQGKGY
HHHHHCCCCCEEEEHHHHHHCCEEEEEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCE
GLFITREIVRHHQGKIEITGNHEGGTTVKIMLPAV
EEEEEHHHHHHCCCEEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure
MVMRRYSFMVDDQLRLVARDDLHRSVGNDPLLGIPYYECYPRLWNGNVDAVRTVVDQGEP
CCEEEEEEEECCCHHEEEHHHHHHHCCCCCEECCCHHHHHHHHCCCCHHHHHHHHHCCCC
LVLNGYRHYCFYGEFSAADIEIRPVFDGASRCTGAMVQVSVLPGCTVYGEMDSARALIDI
EEEECCEEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEEECCCCCCCEEEEC
GKMSVTLAHGVRNPLNAIKGAVVYLKDKYCTDATFAEFADIIDEEICKLDGFITEFLGTS
CCCEEEEHHHCCCHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
HLEPVREEIQLNDLLERVVKFVSLQADANHVRFDVEYGELPLVMLDSFNFGHAILNIVNN
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHH
ALGAMEAGGSLTMRTSTLLEGGVEMIVVEVADTGSGLRSGGKGMLGSFPGAGSRKQGKGY
HHHHHCCCCCEEEEHHHHHHCCEEEEEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCE
GLFITREIVRHHQGKIEITGNHEGGTTVKIMLPAV
EEEEEHHHHHHCCCEEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8095910 [H]