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The map label for this gene is ilvB [H]

Identifier: 39997009

GI number: 39997009

Start: 2089050

End: 2090750

Strand: Reverse

Name: ilvB [H]

Synonym: GSU1911

Alternate gene names: 39997009

Gene position: 2090750-2089050 (Counterclockwise)

Preceding gene: 39997010

Following gene: 39997008

Centisome position: 54.82

GC content: 58.61

Gene sequence:

>1701_bases
ATGAAAATGACAGGAGCGAGAATATTACTCGAATGCCTCAAGCTCGAAGGGGTCGATACCGTGTTCGGCTACCCCGGTGG
CACGGTAATCAACATCTATGATGAGCTGTTTTCTTTCAAGGAGATACGTCACATTCTTCCCCGGCACGAACAGGCCGGCG
TCCACGCGGCCGACGGTTTTGCACGTGCCACCGGTCGGGTGGGGGTAGCCATCGCCACGTCCGGACCCGGAGCCACCAAC
ACCGTCACCGGCATTGCCACCGCATACATGGATTCTATCCCCGTGGTGGTTATTACCGGCCAGGTGCCCACGGCCCTTAT
CGGCAACGATGCGTTCCAGGAAGTCGATATTGTCGGCATCACCCGGCCGTGCACCAAGCATAATTTCCTGGTAAAGGATG
TGAAAGATATCCCGTCCATCATCAAGAAGGCATTCTATATCGCCCGCACGGGCCGTCCGGGGCCGGTCCTCGTGGATCTT
CCCAAGGACGTACAGATCGCCACCGCTGAGTTTCACTACCCGGATTCCATCGAATTGCGGAGCTACAAGCCGACGGTCGA
GGGACATCCCAAACAGATCGAAAAGGCCGTCTCCCTCATGCTTGAGGCCAAGAAGCCGGTGATCTACGTGGGTGGTGGGG
TCATCTCCGGCGATGCAGCCGGCGAACTCTATGATTTCGCCAAAAAACTCAATATGCCGGTTACCACGACCCTCATGGGA
CTCGGCTCCTTTCCCGAGGATGATCATCAGGCCCTGCGACTCCTGGGAATGCACGGGACCTACTACGCGAACATGGCAGT
AACCAACTGCGATATGCTGTTTGCCGTGGGCGCCCGGTTCGACGACCGCGTAACCGGTAAGGTCGCCACCTTCGCCCCCC
ACGCGAAGATCATCCACGTCGACGTGGACCCCACGTCCATCAGGAAGAACGTGCGGGTCGACCTCCCCATCGTGGGCGAG
GTGAAGAAGGTCCTGTCCCAGATGCTCAAGGTGCTGGAGGAGCAGGGGGACAAGGTGGAGCGCTTTCGCACGGGTATTGA
ACCCTGGATGGCCGAGATCGAAGGATGGAAGCAGAAGCATCCCATGACCTACAAGCAGACAACCTCGGTCATCAAGCCTC
AGTACGTTATTCAGAAGCTGCGGGAGCTTTCCGAGCCCGACGCCATCATCGCCACGGACGTGGGGCAGCACCAGATGTGG
ACTGCCCAGTTTTTCACCTTCACCAAAGCCCGGACCCTCCTGTCATCGGGTGGTCTCGGCACCATGGGCTACGGTCTGCC
TGCAGCCATGGGCGCCCAGGCTGCGTATCCCGATCGCCAGGTTATCGCTATATGCGGAGACGGCGGCTTCCAGATGAATA
TGCAGGAACTGGCGACACTGGTCCAGAATCGGTTGCCGGTCAAGATCTGTATTCTCAACAACAATTTCCTCGGCATGGTC
CGGCAGTGGCAGGAGCTGTTCTTCGACCGCCGCTACTCCCAGACCTGCATGGAGCTGCCCATCGACTTCATCAAGCTGGC
AGAGGCCTTCGGTGCGACCGGTCTCCAGGCCTCCAAGGTGGACGAAGTGGAAGGCACCATCAAGAAGGCATTCGAGACGC
CCGGACCGGTTATCATGGAGTTCAAGATCGCGCGGGAGGAAAAGGTCCTCCCCATGGTGCCCGCCGGGGCTTCTCTCAAT
GAAATGGTGCTCAACGCCTAA

Upstream 100 bases:

>100_bases
GCTGGCTCGCCCGCTATGCGAAGGTGGTGACATCCGCCTACACCGGTGCGGTGACCTCGGCCGACTGAAACTTTTTCCAT
TTGCCCGCAGGAGGCTTTGC

Downstream 100 bases:

>100_bases
TCGTGCAAGTCGGGTCGGTCGTCGTCGGTGACGCCCGATCCGCTCTCCCATTCCTCAGGAGGCTTCATACATGCGACATA
CGATCACCGTTCTGGTGGAA

Product: acetolactate synthase large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKMTGARILLECLKLEGVDTVFGYPGGTVINIYDELFSFKEIRHILPRHEQAGVHAADGFARATGRVGVAIATSGPGATN
TVTGIATAYMDSIPVVVITGQVPTALIGNDAFQEVDIVGITRPCTKHNFLVKDVKDIPSIIKKAFYIARTGRPGPVLVDL
PKDVQIATAEFHYPDSIELRSYKPTVEGHPKQIEKAVSLMLEAKKPVIYVGGGVISGDAAGELYDFAKKLNMPVTTTLMG
LGSFPEDDHQALRLLGMHGTYYANMAVTNCDMLFAVGARFDDRVTGKVATFAPHAKIIHVDVDPTSIRKNVRVDLPIVGE
VKKVLSQMLKVLEEQGDKVERFRTGIEPWMAEIEGWKQKHPMTYKQTTSVIKPQYVIQKLRELSEPDAIIATDVGQHQMW
TAQFFTFTKARTLLSSGGLGTMGYGLPAAMGAQAAYPDRQVIAICGDGGFQMNMQELATLVQNRLPVKICILNNNFLGMV
RQWQELFFDRRYSQTCMELPIDFIKLAEAFGATGLQASKVDEVEGTIKKAFETPGPVIMEFKIAREEKVLPMVPAGASLN
EMVLNA

Sequences:

>Translated_566_residues
MKMTGARILLECLKLEGVDTVFGYPGGTVINIYDELFSFKEIRHILPRHEQAGVHAADGFARATGRVGVAIATSGPGATN
TVTGIATAYMDSIPVVVITGQVPTALIGNDAFQEVDIVGITRPCTKHNFLVKDVKDIPSIIKKAFYIARTGRPGPVLVDL
PKDVQIATAEFHYPDSIELRSYKPTVEGHPKQIEKAVSLMLEAKKPVIYVGGGVISGDAAGELYDFAKKLNMPVTTTLMG
LGSFPEDDHQALRLLGMHGTYYANMAVTNCDMLFAVGARFDDRVTGKVATFAPHAKIIHVDVDPTSIRKNVRVDLPIVGE
VKKVLSQMLKVLEEQGDKVERFRTGIEPWMAEIEGWKQKHPMTYKQTTSVIKPQYVIQKLRELSEPDAIIATDVGQHQMW
TAQFFTFTKARTLLSSGGLGTMGYGLPAAMGAQAAYPDRQVIAICGDGGFQMNMQELATLVQNRLPVKICILNNNFLGMV
RQWQELFFDRRYSQTCMELPIDFIKLAEAFGATGLQASKVDEVEGTIKKAFETPGPVIMEFKIAREEKVLPMVPAGASLN
EMVLNA
>Mature_566_residues
MKMTGARILLECLKLEGVDTVFGYPGGTVINIYDELFSFKEIRHILPRHEQAGVHAADGFARATGRVGVAIATSGPGATN
TVTGIATAYMDSIPVVVITGQVPTALIGNDAFQEVDIVGITRPCTKHNFLVKDVKDIPSIIKKAFYIARTGRPGPVLVDL
PKDVQIATAEFHYPDSIELRSYKPTVEGHPKQIEKAVSLMLEAKKPVIYVGGGVISGDAAGELYDFAKKLNMPVTTTLMG
LGSFPEDDHQALRLLGMHGTYYANMAVTNCDMLFAVGARFDDRVTGKVATFAPHAKIIHVDVDPTSIRKNVRVDLPIVGE
VKKVLSQMLKVLEEQGDKVERFRTGIEPWMAEIEGWKQKHPMTYKQTTSVIKPQYVIQKLRELSEPDAIIATDVGQHQMW
TAQFFTFTKARTLLSSGGLGTMGYGLPAAMGAQAAYPDRQVIAICGDGGFQMNMQELATLVQNRLPVKICILNNNFLGMV
RQWQELFFDRRYSQTCMELPIDFIKLAEAFGATGLQASKVDEVEGTIKKAFETPGPVIMEFKIAREEKVLPMVPAGASLN
EMVLNA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=553, Percent_Identity=24.9547920433996, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI93004078, Length=567, Percent_Identity=24.1622574955908, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=49.8239436619718, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=41.6370106761566, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1786717, Length=554, Percent_Identity=36.101083032491, Blast_Score=342, Evalue=6e-95,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=30.2702702702703, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1788716, Length=555, Percent_Identity=26.8468468468468, Blast_Score=161, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17531299, Length=575, Percent_Identity=28.1739130434783, Blast_Score=174, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17531301, Length=575, Percent_Identity=28.1739130434783, Blast_Score=174, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17542570, Length=521, Percent_Identity=26.8714011516315, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=46.483704974271, Blast_Score=505, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6320816, Length=492, Percent_Identity=25.4065040650407, Blast_Score=113, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320123, Length=579, Percent_Identity=23.4887737478411, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=496, Percent_Identity=26.4112903225806, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=545, Percent_Identity=23.1192660550459, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=498, Percent_Identity=25.7028112449799, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922626, Length=571, Percent_Identity=26.7950963222417, Blast_Score=172, Evalue=5e-43,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62080; Mature: 62080

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTGARILLECLKLEGVDTVFGYPGGTVINIYDELFSFKEIRHILPRHEQAGVHAADGF
CCCCHHHHHHHHHHCCCCCEEECCCCCCEEEHHHHHHHHHHHHHHCCCCHHCCCCCCCCH
ARATGRVGVAIATSGPGATNTVTGIATAYMDSIPVVVITGQVPTALIGNDAFQEVDIVGI
HHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEECCCCCHHCEEEEEE
TRPCTKHNFLVKDVKDIPSIIKKAFYIARTGRPGPVLVDLPKDVQIATAEFHYPDSIELR
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCEEEEEEECCCCCCEEE
SYKPTVEGHPKQIEKAVSLMLEAKKPVIYVGGGVISGDAAGELYDFAKKLNMPVTTTLMG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEECCCCHHHHHHHHHCCCCHHHHHHH
LGSFPEDDHQALRLLGMHGTYYANMAVTNCDMLFAVGARFDDRVTGKVATFAPHAKIIHV
CCCCCCCHHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCEEEECCCCEEEEE
DVDPTSIRKNVRVDLPIVGEVKKVLSQMLKVLEEQGDKVERFRTGIEPWMAEIEGWKQKH
ECCHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHCHHHCC
PMTYKQTTSVIKPQYVIQKLRELSEPDAIIATDVGQHQMWTAQFFTFTKARTLLSSGGLG
CCCHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCCC
TMGYGLPAAMGAQAAYPDRQVIAICGDGGFQMNMQELATLVQNRLPVKICILNNNFLGMV
CCCCCCCHHHCCCCCCCCCEEEEEECCCCCEECHHHHHHHHHCCCCEEEEEECCCHHHHH
RQWQELFFDRRYSQTCMELPIDFIKLAEAFGATGLQASKVDEVEGTIKKAFETPGPVIME
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEE
FKIAREEKVLPMVPAGASLNEMVLNA
EEECCCCCCCEECCCCCCHHHHHCCC
>Mature Secondary Structure
MKMTGARILLECLKLEGVDTVFGYPGGTVINIYDELFSFKEIRHILPRHEQAGVHAADGF
CCCCHHHHHHHHHHCCCCCEEECCCCCCEEEHHHHHHHHHHHHHHCCCCHHCCCCCCCCH
ARATGRVGVAIATSGPGATNTVTGIATAYMDSIPVVVITGQVPTALIGNDAFQEVDIVGI
HHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEECCCCCHHCEEEEEE
TRPCTKHNFLVKDVKDIPSIIKKAFYIARTGRPGPVLVDLPKDVQIATAEFHYPDSIELR
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCEEEEEEECCCCCCEEE
SYKPTVEGHPKQIEKAVSLMLEAKKPVIYVGGGVISGDAAGELYDFAKKLNMPVTTTLMG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEECCCCHHHHHHHHHCCCCHHHHHHH
LGSFPEDDHQALRLLGMHGTYYANMAVTNCDMLFAVGARFDDRVTGKVATFAPHAKIIHV
CCCCCCCHHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCEEEECCCCEEEEE
DVDPTSIRKNVRVDLPIVGEVKKVLSQMLKVLEEQGDKVERFRTGIEPWMAEIEGWKQKH
ECCHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHCHHHCC
PMTYKQTTSVIKPQYVIQKLRELSEPDAIIATDVGQHQMWTAQFFTFTKARTLLSSGGLG
CCCHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCCC
TMGYGLPAAMGAQAAYPDRQVIAICGDGGFQMNMQELATLVQNRLPVKICILNNNFLGMV
CCCCCCCHHHCCCCCCCCCEEEEEECCCCCEECHHHHHHHHHCCCCEEEEEECCCHHHHH
RQWQELFFDRRYSQTCMELPIDFIKLAEAFGATGLQASKVDEVEGTIKKAFETPGPVIME
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEE
FKIAREEKVLPMVPAGASLNEMVLNA
EEECCCCCCCEECCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]