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The map label for this gene is leuC [H]

Identifier: 39997001

GI number: 39997001

Start: 2080770

End: 2082053

Strand: Reverse

Name: leuC [H]

Synonym: GSU1903

Alternate gene names: 39997001

Gene position: 2082053-2080770 (Counterclockwise)

Preceding gene: 39997004

Following gene: 39997000

Centisome position: 54.59

GC content: 59.11

Gene sequence:

>1284_bases
ATGGGGAAAACTATTGCGGAAAAGATTTTTGCCAGTCATCTGGTTGATGAACCCTTTGCCGGCACCAAGGTGCTGAGGCT
CGATGTGGTGATGTGTCACGAGATCACCACGCCCATCGCCATTGCCGACCTGATGGCGCGCGGCAAGGATCGGGTTTTCG
ACCCGACAAAGATCAAGGCGGTCATTGATCACGTGACCCCCAGCAAGGACTCCAAGACGGCCACCCAGGCGAAGATGTTG
CGCGATTGGGCGCGGCGCCATGGCATCGTGGACTTTTTCGACGTCGGAGCTAACGGCGTTTGTCATGCACTGTTCCCCGA
GAAGGGGTTCATCCGGCCCGGCTATACGGTGATCATGGGCGATTCGCATACCTGTACTCACGGAGCGTTCGGCGCGTTTG
CGGCAGGCATCGGCACCACCGATCTCGAGGTCGGCATTCTCAAGGGAGTCTGCGCCTTCCGCGAGCCGAAAACGATCCGC
ATCAACCTGAACGGCTCTCTGCCTGAAGGGGTGTATGCCAAGGACGTAATCCTCCATGTCATCGGCAGGATTGGTGTGAA
CGGGGCAACCGACCGGGTAATGGAGTTCCGCGGATCGGTGGTCGACACCATGACCATGGAGTCGCGGATGACACTGTGCA
ACATGGCCATCGAGGCGGGTGGCACCTCGGGTATCTGTATGCCTGACATGGTGACGGTCGATTACCTCTGGCCCTTCCTC
AAGGATGAGTACCAGTCCCGGGAGGCCGCCCTTGCGGCATTCAGCCTGTGGCGGTCCGACGAAGACGCCGTCTACGAGCA
GGTGCTTGATTTTGATGTGTCGAGTCTCGAACCCATTGTCACATTTGGCTATAAGCCGGACCAGGTGAAGCCGGTCTCTG
AAATTGCCGGTACCCCCGTGGACCAGGTTTATCTCGGCTCCTGCACCAACGGCCGGCTGGAGGATCTGCGCATTGCCGCA
CGGATTCTCAAGGGAAAGAAGATCGCACCCACGGTACGCGGCATACTCTCTCCCGCCACGCCGAAGATCTACCAGGATGC
CATGCGTGAGGGGCTGATCGATATCTTCATGGAAGCCGGCTTCTGTGTGACCAATCCCACCTGCGGCGCCTGTCTCGGCA
TGAGCAACGGCGTCCTGGCCGAAGGAGAAGTCTGCGCATCGACCACAAACCGTAACTTCATGGGGCGGATGGGCAAGGGG
GGGATGGTCCACCTCATGTCTCCGGCAACGAGTGCTGCAACCGCTATCGAGGGTAAGATAGCCGACCCCCGGAAGTATCT
GTAG

Upstream 100 bases:

>100_bases
AATGTTAAACCGCGTCGAACAAATATTACATTGATTTATAAACCCCGTTTGTTTACATTGGCACCACATTCCATCATGTT
AGACGCGAGGATAGTTCGAA

Downstream 100 bases:

>100_bases
CTTCCATATTCCCAGCAAGGAGACAGACCGATGAAACAGTTTGGCGGCCCCGTGCTCTTTCTCGACCGCTCTGATATCAA
TACCGACGAGATTATCCCGG

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MGKTIAEKIFASHLVDEPFAGTKVLRLDVVMCHEITTPIAIADLMARGKDRVFDPTKIKAVIDHVTPSKDSKTATQAKML
RDWARRHGIVDFFDVGANGVCHALFPEKGFIRPGYTVIMGDSHTCTHGAFGAFAAGIGTTDLEVGILKGVCAFREPKTIR
INLNGSLPEGVYAKDVILHVIGRIGVNGATDRVMEFRGSVVDTMTMESRMTLCNMAIEAGGTSGICMPDMVTVDYLWPFL
KDEYQSREAALAAFSLWRSDEDAVYEQVLDFDVSSLEPIVTFGYKPDQVKPVSEIAGTPVDQVYLGSCTNGRLEDLRIAA
RILKGKKIAPTVRGILSPATPKIYQDAMREGLIDIFMEAGFCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKG
GMVHLMSPATSAATAIEGKIADPRKYL

Sequences:

>Translated_427_residues
MGKTIAEKIFASHLVDEPFAGTKVLRLDVVMCHEITTPIAIADLMARGKDRVFDPTKIKAVIDHVTPSKDSKTATQAKML
RDWARRHGIVDFFDVGANGVCHALFPEKGFIRPGYTVIMGDSHTCTHGAFGAFAAGIGTTDLEVGILKGVCAFREPKTIR
INLNGSLPEGVYAKDVILHVIGRIGVNGATDRVMEFRGSVVDTMTMESRMTLCNMAIEAGGTSGICMPDMVTVDYLWPFL
KDEYQSREAALAAFSLWRSDEDAVYEQVLDFDVSSLEPIVTFGYKPDQVKPVSEIAGTPVDQVYLGSCTNGRLEDLRIAA
RILKGKKIAPTVRGILSPATPKIYQDAMREGLIDIFMEAGFCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKG
GMVHLMSPATSAATAIEGKIADPRKYL
>Mature_426_residues
GKTIAEKIFASHLVDEPFAGTKVLRLDVVMCHEITTPIAIADLMARGKDRVFDPTKIKAVIDHVTPSKDSKTATQAKMLR
DWARRHGIVDFFDVGANGVCHALFPEKGFIRPGYTVIMGDSHTCTHGAFGAFAAGIGTTDLEVGILKGVCAFREPKTIRI
NLNGSLPEGVYAKDVILHVIGRIGVNGATDRVMEFRGSVVDTMTMESRMTLCNMAIEAGGTSGICMPDMVTVDYLWPFLK
DEYQSREAALAAFSLWRSDEDAVYEQVLDFDVSSLEPIVTFGYKPDQVKPVSEIAGTPVDQVYLGSCTNGRLEDLRIAAR
ILKGKKIAPTVRGILSPATPKIYQDAMREGLIDIFMEAGFCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKGG
MVHLMSPATSAATAIEGKIADPRKYL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=458, Percent_Identity=28.1659388646288, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI41352693, Length=383, Percent_Identity=24.2819843342037, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=38.1974248927039, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI2367097, Length=362, Percent_Identity=25.9668508287293, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=26.5927977839335, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87081781, Length=457, Percent_Identity=23.4135667396061, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25149337, Length=451, Percent_Identity=28.6031042128603, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=348, Percent_Identity=30.1724137931034, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=281, Percent_Identity=30.6049822064057, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=473, Percent_Identity=35.3065539112051, Blast_Score=269, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6320440, Length=456, Percent_Identity=30.7017543859649, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322261, Length=456, Percent_Identity=29.1666666666667, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323335, Length=452, Percent_Identity=27.6548672566372, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI281365315, Length=451, Percent_Identity=27.2727272727273, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17864292, Length=451, Percent_Identity=27.2727272727273, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28571643, Length=454, Percent_Identity=26.6519823788546, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161076999, Length=346, Percent_Identity=28.3236994219653, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 46174; Mature: 46043

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTIAEKIFASHLVDEPFAGTKVLRLDVVMCHEITTPIAIADLMARGKDRVFDPTKIKA
CCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHH
VIDHVTPSKDSKTATQAKMLRDWARRHGIVDFFDVGANGVCHALFPEKGFIRPGYTVIMG
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEC
DSHTCTHGAFGAFAAGIGTTDLEVGILKGVCAFREPKTIRINLNGSLPEGVYAKDVILHV
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHH
IGRIGVNGATDRVMEFRGSVVDTMTMESRMTLCNMAIEAGGTSGICMPDMVTVDYLWPFL
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
KDEYQSREAALAAFSLWRSDEDAVYEQVLDFDVSSLEPIVTFGYKPDQVKPVSEIAGTPV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHCCCCEEEECCCCCCCCCHHHHCCCCC
DQVYLGSCTNGRLEDLRIAARILKGKKIAPTVRGILSPATPKIYQDAMREGLIDIFMEAG
CCEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
FCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKGGMVHLMSPATSAATAIEGKI
CEECCCCCHHHHCCCCCCEECCCEEECCCCCCHHCCCCCCCEEEEECCCCHHHHHHCCCC
ADPRKYL
CCCCCCC
>Mature Secondary Structure 
GKTIAEKIFASHLVDEPFAGTKVLRLDVVMCHEITTPIAIADLMARGKDRVFDPTKIKA
CHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHH
VIDHVTPSKDSKTATQAKMLRDWARRHGIVDFFDVGANGVCHALFPEKGFIRPGYTVIMG
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEC
DSHTCTHGAFGAFAAGIGTTDLEVGILKGVCAFREPKTIRINLNGSLPEGVYAKDVILHV
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHH
IGRIGVNGATDRVMEFRGSVVDTMTMESRMTLCNMAIEAGGTSGICMPDMVTVDYLWPFL
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
KDEYQSREAALAAFSLWRSDEDAVYEQVLDFDVSSLEPIVTFGYKPDQVKPVSEIAGTPV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHCCCCEEEECCCCCCCCCHHHHCCCCC
DQVYLGSCTNGRLEDLRIAARILKGKKIAPTVRGILSPATPKIYQDAMREGLIDIFMEAG
CCEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
FCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKGGMVHLMSPATSAATAIEGKI
CEECCCCCHHHHCCCCCCEECCCEEECCCCCCHHCCCCCCCEEEEECCCCHHHHHHCCCC
ADPRKYL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA