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The map label for this gene is ksgA

Identifier: 39996962

GI number: 39996962

Start: 2040541

End: 2041371

Strand: Reverse

Name: ksgA

Synonym: GSU1864

Alternate gene names: 39996962

Gene position: 2041371-2040541 (Counterclockwise)

Preceding gene: 39996963

Following gene: 39996960

Centisome position: 53.52

GC content: 62.45

Gene sequence:

>831_bases
GTGAGAGGGGACGGCATCCGTGCCCGGAAGGCGCTCGGGCAGAATTTTCTCACTGACCGTTCGGTTCTTTCCCGCATTGC
GGCCCTAGTGAGTGCCGGTGCCGGCGAGCGGATTCTGGAGATCGGCCCCGGCAAGGGAGCTCTGACGTCATATCTGGCGG
AACAGGCCGGACAGCTCGTGGCCGTTGAGTTGGACGACCGTCTGGTGCCGCTCCTGCGCGGCAGTTTTGCCGGAAACCCC
TCAGTTACGATCATCGAAGGTGACATCCTCGACCTGGATCTCCGCGAAACCCTCGGCCGCTACGGCACGCCTCCCTGGAA
GGTGGCGGCAAACCTGCCCTATAACATATCCACGCCGGTCCTGTTCAGACTGCTCGATGCTCGGGATCTATTCAGCCGGC
TCGTACTCATGCTTCAGAAAGAGGTGGGCAACAGACTCGCTGCGGGGCCGGGGAGCAAAGAGTACGGTGTCCTGTCGGTC
CTGTTCCAGCTTCACTTTGATGTGACCCGTGAGATCCTCGTCAGGCCCGGCTCGTTCCATCCTGTGCCCAAGGTGGATTC
GGTGGTTCTCTTGTTTGTTCCCCTGGCGCAGCCACGGGTGGACGTGGGGGACGAGGACTACTTCAGGCGGGTGGTCAAGG
CATCCTTTGCCATGCGCCGCAAGACGCTCTGGAATTGTTTGAAGGGAGGGGCGCTGGGTGTGCCGACGGACGGCATCAGG
GATGTTCTGGCACGCTGCGGCATCGACGAGGGAAGGCGCGGCGAGACGCTGTCCCTGCAGGAGTTTGCCTCATTGACCAA
GGGGCTTCTGGCGGCCGGCGGCTCGCTTTAG

Upstream 100 bases:

>100_bases
TGCCGGGTGACTATTACCTGCGCTGTGGGGAGCAGGGCGGTCTCGCGCTTGACGCACGGGTGAACTGGCCACTTGACCTG
CTCGGGTCGGGGAGGGAGGG

Downstream 100 bases:

>100_bases
CCCCGTGCATCATAGCGGGCGCGGAAAGAAAGAAGCCGTTCGCTGGGCGAACGGCTTCCTAGTGCCACGGGCAGGATATC
GTAATCAGGCAGTAGCTTTC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MRGDGIRARKALGQNFLTDRSVLSRIAALVSAGAGERILEIGPGKGALTSYLAEQAGQLVAVELDDRLVPLLRGSFAGNP
SVTIIEGDILDLDLRETLGRYGTPPWKVAANLPYNISTPVLFRLLDARDLFSRLVLMLQKEVGNRLAAGPGSKEYGVLSV
LFQLHFDVTREILVRPGSFHPVPKVDSVVLLFVPLAQPRVDVGDEDYFRRVVKASFAMRRKTLWNCLKGGALGVPTDGIR
DVLARCGIDEGRRGETLSLQEFASLTKGLLAAGGSL

Sequences:

>Translated_276_residues
MRGDGIRARKALGQNFLTDRSVLSRIAALVSAGAGERILEIGPGKGALTSYLAEQAGQLVAVELDDRLVPLLRGSFAGNP
SVTIIEGDILDLDLRETLGRYGTPPWKVAANLPYNISTPVLFRLLDARDLFSRLVLMLQKEVGNRLAAGPGSKEYGVLSV
LFQLHFDVTREILVRPGSFHPVPKVDSVVLLFVPLAQPRVDVGDEDYFRRVVKASFAMRRKTLWNCLKGGALGVPTDGIR
DVLARCGIDEGRRGETLSLQEFASLTKGLLAAGGSL
>Mature_276_residues
MRGDGIRARKALGQNFLTDRSVLSRIAALVSAGAGERILEIGPGKGALTSYLAEQAGQLVAVELDDRLVPLLRGSFAGNP
SVTIIEGDILDLDLRETLGRYGTPPWKVAANLPYNISTPVLFRLLDARDLFSRLVLMLQKEVGNRLAAGPGSKEYGVLSV
LFQLHFDVTREILVRPGSFHPVPKVDSVVLLFVPLAQPRVDVGDEDYFRRVVKASFAMRRKTLWNCLKGGALGVPTDGIR
DVLARCGIDEGRRGETLSLQEFASLTKGLLAAGGSL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=286, Percent_Identity=32.8671328671329, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI7657198, Length=297, Percent_Identity=30.3030303030303, Blast_Score=113, Evalue=1e-25,
Organism=Escherichia coli, GI1786236, Length=266, Percent_Identity=39.0977443609023, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25146882, Length=190, Percent_Identity=35.7894736842105, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=288, Percent_Identity=28.125, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=239, Percent_Identity=30.9623430962343, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21358017, Length=233, Percent_Identity=33.4763948497854, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21357273, Length=249, Percent_Identity=31.7269076305221, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_GEOSL (Q74C12)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952913.1
- ProteinModelPortal:   Q74C12
- SMR:   Q74C12
- GeneID:   2688537
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1864
- NMPDR:   fig|243231.1.peg.1851
- TIGR:   GSU1864
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   GSUL243231:GSU_1864-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 29861; Mature: 29861

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 15-15 BINDING 17-17 BINDING 42-42 BINDING 63-63 BINDING 88-88 BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGDGIRARKALGQNFLTDRSVLSRIAALVSAGAGERILEIGPGKGALTSYLAEQAGQLV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCEE
AVELDDRLVPLLRGSFAGNPSVTIIEGDILDLDLRETLGRYGTPPWKVAANLPYNISTPV
EEECCCHHHHHHHCCCCCCCCEEEEECCEEECCHHHHHHHCCCCCEEEEECCCCCCCHHH
LFRLLDARDLFSRLVLMLQKEVGNRLAAGPGSKEYGVLSVLFQLHFDVTREILVRPGSFH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
PVPKVDSVVLLFVPLAQPRVDVGDEDYFRRVVKASFAMRRKTLWNCLKGGALGVPTDGIR
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
DVLARCGIDEGRRGETLSLQEFASLTKGLLAAGGSL
HHHHHCCCCCCCCCCEECHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRGDGIRARKALGQNFLTDRSVLSRIAALVSAGAGERILEIGPGKGALTSYLAEQAGQLV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCEE
AVELDDRLVPLLRGSFAGNPSVTIIEGDILDLDLRETLGRYGTPPWKVAANLPYNISTPV
EEECCCHHHHHHHCCCCCCCCEEEEECCEEECCHHHHHHHCCCCCEEEEECCCCCCCHHH
LFRLLDARDLFSRLVLMLQKEVGNRLAAGPGSKEYGVLSVLFQLHFDVTREILVRPGSFH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
PVPKVDSVVLLFVPLAQPRVDVGDEDYFRRVVKASFAMRRKTLWNCLKGGALGVPTDGIR
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
DVLARCGIDEGRRGETLSLQEFASLTKGLLAAGGSL
HHHHHCCCCCCCCCCEECHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA