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The map label for this gene is rph

Identifier: 39996894

GI number: 39996894

Start: 1960791

End: 1961507

Strand: Reverse

Name: rph

Synonym: GSU1795

Alternate gene names: 39996894

Gene position: 1961507-1960791 (Counterclockwise)

Preceding gene: 39996895

Following gene: 39996893

Centisome position: 51.43

GC content: 56.07

Gene sequence:

>717_bases
ATGCGCTCAGACGGTCGCAACCCAGCATCACTCAGAAACATACGGATTACACGAAAGTATCTCAAACACGCCGAAGGCTC
TGTTCTTGTTGAATTCGGTGACACAAAAGTTTTGTGCACCGCCTCAGTGGAGGAGTCGGTGCCCCCCTTTCTGCGCGGCA
AGGGTGGGGGATGGGTAACGGCAGAGTATTCCATGTTGCCGCGTGCCACCCATACGCGATCCCCTCGTGAAGCCGCCAAG
GGGAAGCTGGGGGGAAGGACCCATGAGATCCAGCGTCTCATCGGCCGTTCTTTGCGGGCCGTCACAGATTTGACGCTTCT
TGGCGAACGAAGCGTACTGATTGATTGCGATGTGCTTCAGGCTGATGGAGGGACCCGGACAGCTTCTATTACAGGTGCCT
ACGTCGCACTGGTCGACGCGTTTCAATGGCTCGTATCGCAGGGGACTATTGCGACTCTGCCGGTCCGAGAGGCGGTCGCC
GCTGTCAGTGTTGGGATAGTCGGCGGGGAAGTGTTGCTCGATCTGAACTACATTGAGGACTCGCGAGCCGATGTTGATAT
GAATTTTGTCATGACTTCGTCGGGACGTTTCGTGGAAGTGCAGGGAACCGCAGAGGCTGAACCTTTTACCTGCGCCCAGA
TGGATGATATGCGCTCCCTTGCCATGATCGGCATCGAACGTCTCATGGCCATTCAACAAGAGGTGCTTGAGCAGTGA

Upstream 100 bases:

>100_bases
TGCTCTCAGGCGTACGGCTCTGCCGGCCCTTGCCGCCGAGATTGACAACCTGCATCACATCGTTTAATGTACGCTTTTTT
CCAACTGCCAGAGGTATCTC

Downstream 100 bases:

>100_bases
CCCGGCTGGTGGTAGCAACCCGCAACAAGGGTAAACTGCGAGAGATTGCTGCGATTCTGGACGGGTTGCCTTTCACGTTG
TTGTCGCTCGAGGATTTCCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRSDGRNPASLRNIRITRKYLKHAEGSVLVEFGDTKVLCTASVEESVPPFLRGKGGGWVTAEYSMLPRATHTRSPREAAK
GKLGGRTHEIQRLIGRSLRAVTDLTLLGERSVLIDCDVLQADGGTRTASITGAYVALVDAFQWLVSQGTIATLPVREAVA
AVSVGIVGGEVLLDLNYIEDSRADVDMNFVMTSSGRFVEVQGTAEAEPFTCAQMDDMRSLAMIGIERLMAIQQEVLEQ

Sequences:

>Translated_238_residues
MRSDGRNPASLRNIRITRKYLKHAEGSVLVEFGDTKVLCTASVEESVPPFLRGKGGGWVTAEYSMLPRATHTRSPREAAK
GKLGGRTHEIQRLIGRSLRAVTDLTLLGERSVLIDCDVLQADGGTRTASITGAYVALVDAFQWLVSQGTIATLPVREAVA
AVSVGIVGGEVLLDLNYIEDSRADVDMNFVMTSSGRFVEVQGTAEAEPFTCAQMDDMRSLAMIGIERLMAIQQEVLEQ
>Mature_238_residues
MRSDGRNPASLRNIRITRKYLKHAEGSVLVEFGDTKVLCTASVEESVPPFLRGKGGGWVTAEYSMLPRATHTRSPREAAK
GKLGGRTHEIQRLIGRSLRAVTDLTLLGERSVLIDCDVLQADGGTRTASITGAYVALVDAFQWLVSQGTIATLPVREAVA
AVSVGIVGGEVLLDLNYIEDSRADVDMNFVMTSSGRFVEVQGTAEAEPFTCAQMDDMRSLAMIGIERLMAIQQEVLEQ

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=69.0476190476191, Blast_Score=297, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI71981632, Length=193, Percent_Identity=30.5699481865285, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_GEOSL (Q74C79)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952845.1
- ProteinModelPortal:   Q74C79
- SMR:   Q74C79
- GeneID:   2686347
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1795
- NMPDR:   fig|243231.1.peg.1783
- TIGR:   GSU1795
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   GSUL243231:GSU_1795-MONOMER
- BRENDA:   2.7.7.56
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25813; Mature: 25813

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDGRNPASLRNIRITRKYLKHAEGSVLVEFGDTKVLCTASVEESVPPFLRGKGGGWVT
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCHHCCCCHHCCCCCCEEE
AEYSMLPRATHTRSPREAAKGKLGGRTHEIQRLIGRSLRAVTDLTLLGERSVLIDCDVLQ
EEHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEEEE
ADGGTRTASITGAYVALVDAFQWLVSQGTIATLPVREAVAAVSVGIVGGEVLLDLNYIED
CCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHEEECCEEEEEEEECCC
SRADVDMNFVMTSSGRFVEVQGTAEAEPFTCAQMDDMRSLAMIGIERLMAIQQEVLEQ
CCCCCEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSDGRNPASLRNIRITRKYLKHAEGSVLVEFGDTKVLCTASVEESVPPFLRGKGGGWVT
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCHHCCCCHHCCCCCCEEE
AEYSMLPRATHTRSPREAAKGKLGGRTHEIQRLIGRSLRAVTDLTLLGERSVLIDCDVLQ
EEHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEEEE
ADGGTRTASITGAYVALVDAFQWLVSQGTIATLPVREAVAAVSVGIVGGEVLLDLNYIED
CCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHEEECCEEEEEEEECCC
SRADVDMNFVMTSSGRFVEVQGTAEAEPFTCAQMDDMRSLAMIGIERLMAIQQEVLEQ
CCCCCEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA