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The map label for this gene is livK [C]

Identifier: 39996834

GI number: 39996834

Start: 1900164

End: 1901300

Strand: Reverse

Name: livK [C]

Synonym: GSU1735

Alternate gene names: 39996834

Gene position: 1901300-1900164 (Counterclockwise)

Preceding gene: 39996835

Following gene: 39996833

Centisome position: 49.85

GC content: 56.64

Gene sequence:

>1137_bases
ATGAACAGAATCTTCGCTGTTATCGTCGGTATCGTCGGTCTGTTGGTGGCCGGCATCGCCTCGGCAGCAGAACCGTTCAG
GATCGGAGCGCTTTTTTCCATAACCGGTCCCGCGTCATTTCTGGGAGAGCCCGAGAAGAATACCCTGGAGATGCTGGTGA
AGGACGCCAACGCCAGGGGTGGGATTGGCGGCCGCAAACTTGAGCTGGTGGTCTACGACACCCAGGGGGACGTGACCAAG
GCGGTTCAACTGGCCAACAAACTGATCAAGAACGACAAAGTGAGTGTCATCGTCGGACCGAGTACCACCGGCGAAACCAT
GGCGGTAATTCCCTTGGCGGAAAAGGAGAAAATCCCGCTCATTTCCTGCGCTGCGGGCATCAAGATTACCGATCCGGTCA
AAAAGTGGGTATTCAAAACCCCGGCCAATGACCATGTGGCTGCCGAAAAGATCCTGATCCAGGCGGCCAGGCTGAAGCAG
AAGTCTCTTGCCATTATCACCGTTTCCGACGGGTTCGGCTCCTCTGGGCGTGAACAGCTCAAGGCCCTGGCCGGCCAGAA
GGGCTTCAGAATTGTGGCCGATGAGGTGTACGGTCCCAAGGACACCGACATGACCGCCCAACTCACCAAGATCAAGGCGG
CCAAGCCCGACGCGGTCATCTGCTGGGGAACAAATCCCGGTCCTGCACTCATTGCCCGCAACATGAAACAACTCGGGATC
AAGGTCCCGCTCTACATGAGCCACGGTGTGGCGTCCAAAAAGTTCATCGAACTGGCCGGGGCCGATGCCGCCGAAGGGAT
TATGTTGCCGGCGGGCAAGCTTGCCATTTTCGACAAGCTCCCCAAGAACGATCCCCAGTACAAGTTGCTCAAATCGTATG
ATGAATCCTATCGCAAGGCATACGGCGTGGAGGCTTCGACCTTCGGTGGTTACGCCTATGATTCCTTCCTCCTGATTTCC
GGCGCCATGAAAAAGGGAACCTCTCCCCAGCAGATCAGGGACGGCCTTGAGCAGACCCGGAAGCTCGTCAGCGTCTCGGG
GATTTTCACCATGTCCCCCTCGGATCATAACGGTCTGGATCTTTCGGCATTCGAGATGGTCCGCGTGACCAAAGGCGATT
GGGAACTGATCAAATAA

Upstream 100 bases:

>100_bases
TGAGACAGAGAAGGCCATCACAGCCCTTATCAACAACGGATTTACTGTCCTCGAGGGCGAACGCCTGTACGCAATGTAGC
CACTTCAGGGAGGGGGGAGC

Downstream 100 bases:

>100_bases
CTCATGGGGAGGGGTGTGATGCTGAAAAGAGGATCCTTTATCCTGACGCTCGCCGCATCGTTCATGATGCTGGCTGCCAA
CGTATTTGCCGCGTCACCCA

Product: branched-chain amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MNRIFAVIVGIVGLLVAGIASAAEPFRIGALFSITGPASFLGEPEKNTLEMLVKDANARGGIGGRKLELVVYDTQGDVTK
AVQLANKLIKNDKVSVIVGPSTTGETMAVIPLAEKEKIPLISCAAGIKITDPVKKWVFKTPANDHVAAEKILIQAARLKQ
KSLAIITVSDGFGSSGREQLKALAGQKGFRIVADEVYGPKDTDMTAQLTKIKAAKPDAVICWGTNPGPALIARNMKQLGI
KVPLYMSHGVASKKFIELAGADAAEGIMLPAGKLAIFDKLPKNDPQYKLLKSYDESYRKAYGVEASTFGGYAYDSFLLIS
GAMKKGTSPQQIRDGLEQTRKLVSVSGIFTMSPSDHNGLDLSAFEMVRVTKGDWELIK

Sequences:

>Translated_378_residues
MNRIFAVIVGIVGLLVAGIASAAEPFRIGALFSITGPASFLGEPEKNTLEMLVKDANARGGIGGRKLELVVYDTQGDVTK
AVQLANKLIKNDKVSVIVGPSTTGETMAVIPLAEKEKIPLISCAAGIKITDPVKKWVFKTPANDHVAAEKILIQAARLKQ
KSLAIITVSDGFGSSGREQLKALAGQKGFRIVADEVYGPKDTDMTAQLTKIKAAKPDAVICWGTNPGPALIARNMKQLGI
KVPLYMSHGVASKKFIELAGADAAEGIMLPAGKLAIFDKLPKNDPQYKLLKSYDESYRKAYGVEASTFGGYAYDSFLLIS
GAMKKGTSPQQIRDGLEQTRKLVSVSGIFTMSPSDHNGLDLSAFEMVRVTKGDWELIK
>Mature_378_residues
MNRIFAVIVGIVGLLVAGIASAAEPFRIGALFSITGPASFLGEPEKNTLEMLVKDANARGGIGGRKLELVVYDTQGDVTK
AVQLANKLIKNDKVSVIVGPSTTGETMAVIPLAEKEKIPLISCAAGIKITDPVKKWVFKTPANDHVAAEKILIQAARLKQ
KSLAIITVSDGFGSSGREQLKALAGQKGFRIVADEVYGPKDTDMTAQLTKIKAAKPDAVICWGTNPGPALIARNMKQLGI
KVPLYMSHGVASKKFIELAGADAAEGIMLPAGKLAIFDKLPKNDPQYKLLKSYDESYRKAYGVEASTFGGYAYDSFLLIS
GAMKKGTSPQQIRDGLEQTRKLVSVSGIFTMSPSDHNGLDLSAFEMVRVTKGDWELIK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=285, Percent_Identity=26.3157894736842, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789867, Length=288, Percent_Identity=28.4722222222222, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40440; Mature: 40440

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIFAVIVGIVGLLVAGIASAAEPFRIGALFSITGPASFLGEPEKNTLEMLVKDANARG
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHCCCCCHHHHHHHHCCCCCCC
GIGGRKLELVVYDTQGDVTKAVQLANKLIKNDKVSVIVGPSTTGETMAVIPLAEKEKIPL
CCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCE
ISCAAGIKITDPVKKWVFKTPANDHVAAEKILIQAARLKQKSLAIITVSDGFGSSGREQL
EEECCCCEECCHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
KALAGQKGFRIVADEVYGPKDTDMTAQLTKIKAAKPDAVICWGTNPGPALIARNMKQLGI
HHHHCCCCCEEEEECCCCCCCCCCHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHCCC
KVPLYMSHGVASKKFIELAGADAAEGIMLPAGKLAIFDKLPKNDPQYKLLKSYDESYRKA
EEEEEECCCCCHHHHHEECCCCCCCCEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
YGVEASTFGGYAYDSFLLISGAMKKGTSPQQIRDGLEQTRKLVSVSGIFTMSPSDHNGLD
HCCCCCCCCCHHHHHHHEEEHHHHCCCCHHHHHHHHHHHHHHHEECEEEEECCCCCCCCC
LSAFEMVRVTKGDWELIK
HHHEEEEEEECCCEEECC
>Mature Secondary Structure
MNRIFAVIVGIVGLLVAGIASAAEPFRIGALFSITGPASFLGEPEKNTLEMLVKDANARG
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHCCCCCHHHHHHHHCCCCCCC
GIGGRKLELVVYDTQGDVTKAVQLANKLIKNDKVSVIVGPSTTGETMAVIPLAEKEKIPL
CCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCE
ISCAAGIKITDPVKKWVFKTPANDHVAAEKILIQAARLKQKSLAIITVSDGFGSSGREQL
EEECCCCEECCHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
KALAGQKGFRIVADEVYGPKDTDMTAQLTKIKAAKPDAVICWGTNPGPALIARNMKQLGI
HHHHCCCCCEEEEECCCCCCCCCCHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHCCC
KVPLYMSHGVASKKFIELAGADAAEGIMLPAGKLAIFDKLPKNDPQYKLLKSYDESYRKA
EEEEEECCCCCHHHHHEECCCCCCCCEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
YGVEASTFGGYAYDSFLLISGAMKKGTSPQQIRDGLEQTRKLVSVSGIFTMSPSDHNGLD
HCCCCCCCCCHHHHHHHEEEHHHHCCCCHHHHHHHHHHHHHHHEECEEEEECCCCCCCCC
LSAFEMVRVTKGDWELIK
HHHEEEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA