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The map label for this gene is livK [C]

Identifier: 39996833

GI number: 39996833

Start: 1899003

End: 1900145

Strand: Reverse

Name: livK [C]

Synonym: GSU1734

Alternate gene names: 39996833

Gene position: 1900145-1899003 (Counterclockwise)

Preceding gene: 39996834

Following gene: 39996832

Centisome position: 49.82

GC content: 59.76

Gene sequence:

>1143_bases
ATGCTGAAAAGAGGATCCTTTATCCTGACGCTCGCCGCATCGTTCATGATGCTGGCTGCCAACGTATTTGCCGCGTCACC
CATCCGGATAGGCGCACTCTTCGCCGTAACCGGACCGGCGTCGTTCCTGGGGGAACCGGAACGTAACACCGCCCAGATGA
TGGTCAACAAGATCAACGCCGCCGGCGGTATCAAGGGAAGAAAAGTCGAACTGATCGTCTATGACACGCAAGGCGACGCC
ACCAAGGCCGTGCAGGCGGCCAACCGGCTCATCAAGGACGACAAGGTGGTTGCCATCATCGGCCCCAGCACGACCGGGGA
CACCATGGCGGTCATCCCTATCGTGGAAAAGGCCCAGATTCCCCTCGTTTCCTGTGCTGCCGGCAGCAAGATCACCGAAC
CGGTGAAAAAATGGGTCTTCAAGACTGCCCAGAACGACGGGCTTGCAGTGGCAAAGATCTATGAACAACTCAACCGCCTC
AGGATGAGCAAAGTGGCGATCCTGACGGTATCCGACGGGTTCGGCTCTTCCGGGCGCGAGCAACTCAAGGCCCAGGCGGC
GCAACACGGCATCCAGATCGTTTCCGATGACACGTATGGGCCCAAGGACACGGATATGACGGCGCAGCTTACCAAGATCC
GCAGCAGCGGCGCCCAGGCCGTGATCTGCTGGGGCACCAACCCCGGCCCTGCCGTCATTGCCCGCAACGCCAAGCAACTC
GGGCTCGCCATTCCCATCTACATGAGTCATGGCGTGTCATCCAAGAAGTTCATCGAGCTTGCCGGCGATGCAGCCGAAGG
AATCATTCTGCCGTCGGGCCGGGTCATCGTCGCCGACCTGCTTCCCGCCGCGGACAAGCAGAAGAAGTCGCTCATGGCAT
ACGTCAAGGATTACCAGAAGCAGTTCAAGGCCGAGGGTGACCATTTTGGCGGCCATGCCTGGGATGCGGTGATGTTGCTG
AAAGGTGCCCTGGAGCGTGGGGGTGACAGGCCTGCCGCCATCCGCGACCAGTTGGAGAAGACCAGAAACTTCGCGGGTAT
TGGAGGCATCTTCAACTACTCCGCCGCCGACCATGCCGGCCTCGGCAAGGATGCCTTCGTCCTCGTGACGATCCACAACA
AGGACTTTGCTCTCGTCAAGTAA

Upstream 100 bases:

>100_bases
CCCCTCGGATCATAACGGTCTGGATCTTTCGGCATTCGAGATGGTCCGCGTGACCAAAGGCGATTGGGAACTGATCAAAT
AACTCATGGGGAGGGGTGTG

Downstream 100 bases:

>100_bases
GGTCCTTGCCGCACCATTGCGATCGAACCTTGGGAGCGCAGGCTGTCCCGGCCGCGCTCCCTCATTGTGAACCGGATGTA
TACAGCATGAATTTAGGCCA

Product: branched-chain amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MLKRGSFILTLAASFMMLAANVFAASPIRIGALFAVTGPASFLGEPERNTAQMMVNKINAAGGIKGRKVELIVYDTQGDA
TKAVQAANRLIKDDKVVAIIGPSTTGDTMAVIPIVEKAQIPLVSCAAGSKITEPVKKWVFKTAQNDGLAVAKIYEQLNRL
RMSKVAILTVSDGFGSSGREQLKAQAAQHGIQIVSDDTYGPKDTDMTAQLTKIRSSGAQAVICWGTNPGPAVIARNAKQL
GLAIPIYMSHGVSSKKFIELAGDAAEGIILPSGRVIVADLLPAADKQKKSLMAYVKDYQKQFKAEGDHFGGHAWDAVMLL
KGALERGGDRPAAIRDQLEKTRNFAGIGGIFNYSAADHAGLGKDAFVLVTIHNKDFALVK

Sequences:

>Translated_380_residues
MLKRGSFILTLAASFMMLAANVFAASPIRIGALFAVTGPASFLGEPERNTAQMMVNKINAAGGIKGRKVELIVYDTQGDA
TKAVQAANRLIKDDKVVAIIGPSTTGDTMAVIPIVEKAQIPLVSCAAGSKITEPVKKWVFKTAQNDGLAVAKIYEQLNRL
RMSKVAILTVSDGFGSSGREQLKAQAAQHGIQIVSDDTYGPKDTDMTAQLTKIRSSGAQAVICWGTNPGPAVIARNAKQL
GLAIPIYMSHGVSSKKFIELAGDAAEGIILPSGRVIVADLLPAADKQKKSLMAYVKDYQKQFKAEGDHFGGHAWDAVMLL
KGALERGGDRPAAIRDQLEKTRNFAGIGGIFNYSAADHAGLGKDAFVLVTIHNKDFALVK
>Mature_380_residues
MLKRGSFILTLAASFMMLAANVFAASPIRIGALFAVTGPASFLGEPERNTAQMMVNKINAAGGIKGRKVELIVYDTQGDA
TKAVQAANRLIKDDKVVAIIGPSTTGDTMAVIPIVEKAQIPLVSCAAGSKITEPVKKWVFKTAQNDGLAVAKIYEQLNRL
RMSKVAILTVSDGFGSSGREQLKAQAAQHGIQIVSDDTYGPKDTDMTAQLTKIRSSGAQAVICWGTNPGPAVIARNAKQL
GLAIPIYMSHGVSSKKFIELAGDAAEGIILPSGRVIVADLLPAADKQKKSLMAYVKDYQKQFKAEGDHFGGHAWDAVMLL
KGALERGGDRPAAIRDQLEKTRNFAGIGGIFNYSAADHAGLGKDAFVLVTIHNKDFALVK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=343, Percent_Identity=27.6967930029155, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI48994941, Length=269, Percent_Identity=25.2788104089219, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40363; Mature: 40363

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRGSFILTLAASFMMLAANVFAASPIRIGALFAVTGPASFLGEPERNTAQMMVNKINA
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHCCCCCCCHHHHHHHHHHH
AGGIKGRKVELIVYDTQGDATKAVQAANRLIKDDKVVAIIGPSTTGDTMAVIPIVEKAQI
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCC
PLVSCAAGSKITEPVKKWVFKTAQNDGLAVAKIYEQLNRLRMSKVAILTVSDGFGSSGRE
CEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHH
QLKAQAAQHGIQIVSDDTYGPKDTDMTAQLTKIRSSGAQAVICWGTNPGPAVIARNAKQL
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEECCHHHC
GLAIPIYMSHGVSSKKFIELAGDAAEGIILPSGRVIVADLLPAADKQKKSLMAYVKDYQK
CEEEEEEECCCCCCCCEEEECCCCCCCEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
QFKAEGDHFGGHAWDAVMLLKGALERGGDRPAAIRDQLEKTRNFAGIGGIFNYSAADHAG
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCC
LGKDAFVLVTIHNKDFALVK
CCCCEEEEEEEECCCEEEEC
>Mature Secondary Structure
MLKRGSFILTLAASFMMLAANVFAASPIRIGALFAVTGPASFLGEPERNTAQMMVNKINA
CCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHCCCCCCCHHHHHHHHHHH
AGGIKGRKVELIVYDTQGDATKAVQAANRLIKDDKVVAIIGPSTTGDTMAVIPIVEKAQI
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCC
PLVSCAAGSKITEPVKKWVFKTAQNDGLAVAKIYEQLNRLRMSKVAILTVSDGFGSSGRE
CEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHH
QLKAQAAQHGIQIVSDDTYGPKDTDMTAQLTKIRSSGAQAVICWGTNPGPAVIARNAKQL
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEECCHHHC
GLAIPIYMSHGVSSKKFIELAGDAAEGIILPSGRVIVADLLPAADKQKKSLMAYVKDYQK
CEEEEEEECCCCCCCCEEEECCCCCCCEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
QFKAEGDHFGGHAWDAVMLLKGALERGGDRPAAIRDQLEKTRNFAGIGGIFNYSAADHAG
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCC
LGKDAFVLVTIHNKDFALVK
CCCCEEEEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]