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The map label for this gene is rpfG [H]

Identifier: 39996754

GI number: 39996754

Start: 1812034

End: 1813083

Strand: Reverse

Name: rpfG [H]

Synonym: GSU1654

Alternate gene names: 39996754

Gene position: 1813083-1812034 (Counterclockwise)

Preceding gene: 39996755

Following gene: 39996750

Centisome position: 47.54

GC content: 57.24

Gene sequence:

>1050_bases
ATGAAAGCCACAATCCTGACGGTCGACGATGAGGAGATGATCAGGGACCTGCTGGTGACGGCGCTGTCCCGCGAGGGCTA
CGACTGTTTCCAGGCCGGAAGTGCCGACGAAGCGCGGGATGTGTTGCTGAACAACCGGGTGGAACTCGCCCTGCTGGATA
TCATGATGCCCGGCACATCCGGGGTCGATTTGCTCAAGGAAATAAAGAAGATCAGTGCTGACACGGTTGTGCTGATGGTA
ACCGCCATCAGCGACATGGAAACGGCCATGCGCTGCATCCACCTGGGCGCCGATGACTACATTCTGAAACCCTTCGACAT
CGAGCGGGTTCTGCTCACCATCAAAAACTCCCTGGAAAAGCAGCGCCTGCTGATTGAGAACCGGGAGTACCAAGCAAATC
TTGAGAAAAAGGTTCTGGAACAGACGGGACAAATCCGCTCGGCCATGGAGGAACTCAACCTCACCTACGACCACACGCTC
ACAACGCTCGTGCGTGCTCTGGATGCCCGCGAAAAGGAAACCGGCTCCCACTCGGAACGAGTCATGAACTACACGATTCT
CCTTGCGGAAACCATGGGAATTCGTGCTGCAGAGGTGGCAATCATGGCCCGGGGGGCGCTCCTGCACGACATCGGCAAGA
TTGGCGTCTCAGACAACATACTGCTCAAACCGGCAAAACTCGACGAAGTTGAATGGCGCCACATGAAACAGCACCCGCAG
ATGGGATACGACATCCTCTCAGGGATCAAGTTCCTCAAGGCTCCGGCGGAAGTGGTCTACGCCCACCATGAGCGTTACGA
CGGAACCGGCTACCCAAAAGGGTTGCGCGGAAGCGAAATCCCGCTCGGCGCCCGGATATTCTCCCTGGTGGACACTCTTG
ACGCCATGACCTCGGACAGGCCCTACCGCAGGGCACTGCCGTTTTCCGCAGTGTTGGCCGAAGTCGAGCGCTGCATCGGC
AGTCAGTTCGACCCCTCGATTGCCGCTACGTTCCTGTCCATTCCAAAGGATAAATGGGAAAAGGCGGGCAAGCGGCTGTT
TCTTGACTAG

Upstream 100 bases:

>100_bases
TTCATCATGAAGCCGTTCACCCCCGATGCGCTGGCCGGAAAAGTTCGCGAATTGCTCGGCACTGTTTCGTGATTATACCG
GCATACAGGAGGGAAACCAC

Downstream 100 bases:

>100_bases
GGGAAGTCCCGCATAGTCCCAGATCATCCCGGAACCTGCAACGCGAGCCCCTTAGCGCAACGGACCGCCCGTACGACTCG
TGACCAACCACCGGCGATAC

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKATILTVDDEEMIRDLLVTALSREGYDCFQAGSADEARDVLLNNRVELALLDIMMPGTSGVDLLKEIKKISADTVVLMV
TAISDMETAMRCIHLGADDYILKPFDIERVLLTIKNSLEKQRLLIENREYQANLEKKVLEQTGQIRSAMEELNLTYDHTL
TTLVRALDAREKETGSHSERVMNYTILLAETMGIRAAEVAIMARGALLHDIGKIGVSDNILLKPAKLDEVEWRHMKQHPQ
MGYDILSGIKFLKAPAEVVYAHHERYDGTGYPKGLRGSEIPLGARIFSLVDTLDAMTSDRPYRRALPFSAVLAEVERCIG
SQFDPSIAATFLSIPKDKWEKAGKRLFLD

Sequences:

>Translated_349_residues
MKATILTVDDEEMIRDLLVTALSREGYDCFQAGSADEARDVLLNNRVELALLDIMMPGTSGVDLLKEIKKISADTVVLMV
TAISDMETAMRCIHLGADDYILKPFDIERVLLTIKNSLEKQRLLIENREYQANLEKKVLEQTGQIRSAMEELNLTYDHTL
TTLVRALDAREKETGSHSERVMNYTILLAETMGIRAAEVAIMARGALLHDIGKIGVSDNILLKPAKLDEVEWRHMKQHPQ
MGYDILSGIKFLKAPAEVVYAHHERYDGTGYPKGLRGSEIPLGARIFSLVDTLDAMTSDRPYRRALPFSAVLAEVERCIG
SQFDPSIAATFLSIPKDKWEKAGKRLFLD
>Mature_349_residues
MKATILTVDDEEMIRDLLVTALSREGYDCFQAGSADEARDVLLNNRVELALLDIMMPGTSGVDLLKEIKKISADTVVLMV
TAISDMETAMRCIHLGADDYILKPFDIERVLLTIKNSLEKQRLLIENREYQANLEKKVLEQTGQIRSAMEELNLTYDHTL
TTLVRALDAREKETGSHSERVMNYTILLAETMGIRAAEVAIMARGALLHDIGKIGVSDNILLKPAKLDEVEWRHMKQHPQ
MGYDILSGIKFLKAPAEVVYAHHERYDGTGYPKGLRGSEIPLGARIFSLVDTLDAMTSDRPYRRALPFSAVLAEVERCIG
SQFDPSIAATFLSIPKDKWEKAGKRLFLD

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=130, Percent_Identity=33.0769230769231, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=35.2459016393443, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790299, Length=132, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=31.6239316239316, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788394, Length=195, Percent_Identity=29.2307692307692, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790863, Length=116, Percent_Identity=31.0344827586207, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=34.2105263157895, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787229, Length=118, Percent_Identity=31.3559322033898, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786784, Length=128, Percent_Identity=25.78125, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693157, Length=118, Percent_Identity=32.2033898305085, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 39137; Mature: 39137

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATILTVDDEEMIRDLLVTALSREGYDCFQAGSADEARDVLLNNRVELALLDIMMPGTS
CCEEEEEECHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCHHEEEEEEECCCCC
GVDLLKEIKKISADTVVLMVTAISDMETAMRCIHLGADDYILKPFDIERVLLTIKNSLEK
CHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHH
QRLLIENREYQANLEKKVLEQTGQIRSAMEELNLTYDHTLTTLVRALDAREKETGSHSER
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
VMNYTILLAETMGIRAAEVAIMARGALLHDIGKIGVSDNILLKPAKLDEVEWRHMKQHPQ
HHHHHEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCC
MGYDILSGIKFLKAPAEVVYAHHERYDGTGYPKGLRGSEIPLGARIFSLVDTLDAMTSDR
CCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PYRRALPFSAVLAEVERCIGSQFDPSIAATFLSIPKDKWEKAGKRLFLD
CHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKATILTVDDEEMIRDLLVTALSREGYDCFQAGSADEARDVLLNNRVELALLDIMMPGTS
CCEEEEEECHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCHHEEEEEEECCCCC
GVDLLKEIKKISADTVVLMVTAISDMETAMRCIHLGADDYILKPFDIERVLLTIKNSLEK
CHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHH
QRLLIENREYQANLEKKVLEQTGQIRSAMEELNLTYDHTLTTLVRALDAREKETGSHSER
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
VMNYTILLAETMGIRAAEVAIMARGALLHDIGKIGVSDNILLKPAKLDEVEWRHMKQHPQ
HHHHHEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCC
MGYDILSGIKFLKAPAEVVYAHHERYDGTGYPKGLRGSEIPLGARIFSLVDTLDAMTSDR
CCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PYRRALPFSAVLAEVERCIGSQFDPSIAATFLSIPKDKWEKAGKRLFLD
CHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]