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The map label for this gene is pleD [H]

Identifier: 39996743

GI number: 39996743

Start: 1801850

End: 1802800

Strand: Direct

Name: pleD [H]

Synonym: GSU1643

Alternate gene names: 39996743

Gene position: 1801850-1802800 (Clockwise)

Preceding gene: 39996741

Following gene: 39996744

Centisome position: 47.24

GC content: 58.89

Gene sequence:

>951_bases
ATGACGGATGAACAGAGACAATGCCCGTATCCTGTGCTCATAGCCGAGGATAATGCCCTACTGAGAGCGGTCCTCGAGGG
GAATCTGCGCGAGATCGGGTACGACGTGGTTGTTGCCATTGACGGCAAGGATGCTCTCGCGCGTATTCAAAGTGGCTATT
TCCCTCTGGTTATTACCGACTGGGTCATGCCGGTCATGGACGGGCCCGAACTTTGCCGGGCCGTACGGAGCCTTGGTCTT
GAGCACTATACCTACCTGATCCTCCTCACCTCCCGCGACTCCAAGGAGAGCATCATTTCAGGGCTCGAAGCCGGCGCCGA
CGAGTATCTAGTGAAGCCGGTGACTCCTGAAGAGCTCACCGTCCGGCTCATGACCGCCCGTCGTATTATCGATCTGGAAA
GCTCGCTCAAGCAAAGCATGGAGGAGGTCCGGCTCCTGACCATGCGTGACCCGCTCACGGGTATCTACAACAGGCGCTAC
CTGGAAGACCGGCTGCACCAGGAGGTCAAACGCACCTTCCGCTACGAACGCCCCATCTCGGTAGTCATGTTCGACATTGA
TCACTTCAAGAGGGTGAACGACACCTGGGGACACCTTGTCGGCGACCAGGTCCTGAAGGCCTGTGCCGAATCGGTCCGCT
CCGGGGTCCGCGAAAACATCGACTGGCCCGTCCGTTACGGTGGCGAGGAGTTTGTGGTGGTACTTCCTGAAACCGATATG
GCAGGTGCGGTTATTGTGGCGGAACGGCTCAGGCAACGCATAGCCCTGATACAGACCTCTGTGGGCGATGGGGCCGTGAC
GGTTACAGCCAGCTTTGGCGTGGCCAGTTTTACCCCTCCGGATCAGAAGGAGGATCTCTCCATCGGTGAAGTGCTGCTGG
AGCGAGCCGACCGCTGCCTCTATCGGGCTAAGGGGGAGGGGAGAAACCGGGTGTGCAGCGAACAACTCTGA

Upstream 100 bases:

>100_bases
GTAACTGCTTCCGTTTAAACGGTAGCGAACAATGGAGCGATGTCAAACATGATGGCTGCAGGGGGAGGGGACTCTCTCTC
CAGCGGCTAGGAGTGGTCAG

Downstream 100 bases:

>100_bases
GACGGCCAGATTGAGCTCTTCATTTTCCGGGGCCGCCGGTCGTGCCGGCGGTCTTTGTGCGTTCGGCGACTCCGACGTTG
ACATGCCACGGGCGCCGGGG

Product: GGDEF/response regulator receiver domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTDEQRQCPYPVLIAEDNALLRAVLEGNLREIGYDVVVAIDGKDALARIQSGYFPLVITDWVMPVMDGPELCRAVRSLGL
EHYTYLILLTSRDSKESIISGLEAGADEYLVKPVTPEELTVRLMTARRIIDLESSLKQSMEEVRLLTMRDPLTGIYNRRY
LEDRLHQEVKRTFRYERPISVVMFDIDHFKRVNDTWGHLVGDQVLKACAESVRSGVRENIDWPVRYGGEEFVVVLPETDM
AGAVIVAERLRQRIALIQTSVGDGAVTVTASFGVASFTPPDQKEDLSIGEVLLERADRCLYRAKGEGRNRVCSEQL

Sequences:

>Translated_316_residues
MTDEQRQCPYPVLIAEDNALLRAVLEGNLREIGYDVVVAIDGKDALARIQSGYFPLVITDWVMPVMDGPELCRAVRSLGL
EHYTYLILLTSRDSKESIISGLEAGADEYLVKPVTPEELTVRLMTARRIIDLESSLKQSMEEVRLLTMRDPLTGIYNRRY
LEDRLHQEVKRTFRYERPISVVMFDIDHFKRVNDTWGHLVGDQVLKACAESVRSGVRENIDWPVRYGGEEFVVVLPETDM
AGAVIVAERLRQRIALIQTSVGDGAVTVTASFGVASFTPPDQKEDLSIGEVLLERADRCLYRAKGEGRNRVCSEQL
>Mature_315_residues
TDEQRQCPYPVLIAEDNALLRAVLEGNLREIGYDVVVAIDGKDALARIQSGYFPLVITDWVMPVMDGPELCRAVRSLGLE
HYTYLILLTSRDSKESIISGLEAGADEYLVKPVTPEELTVRLMTARRIIDLESSLKQSMEEVRLLTMRDPLTGIYNRRYL
EDRLHQEVKRTFRYERPISVVMFDIDHFKRVNDTWGHLVGDQVLKACAESVRSGVRENIDWPVRYGGEEFVVVLPETDMA
GAVIVAERLRQRIALIQTSVGDGAVTVTASFGVASFTPPDQKEDLSIGEVLLERADRCLYRAKGEGRNRVCSEQL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=190, Percent_Identity=37.3684210526316, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=40, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=33.8983050847458, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082007, Length=196, Percent_Identity=34.6938775510204, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=36.4161849710983, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787816, Length=174, Percent_Identity=35.0574712643678, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=31.0734463276836, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788085, Length=184, Percent_Identity=32.6086956521739, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788191, Length=112, Percent_Identity=38.3928571428571, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=30.1136363636364, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081974, Length=118, Percent_Identity=29.6610169491525, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790863, Length=139, Percent_Identity=38.1294964028777, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082012, Length=155, Percent_Identity=31.6129032258064, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787056, Length=203, Percent_Identity=26.6009852216749, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788381, Length=164, Percent_Identity=30.4878048780488, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35675; Mature: 35544

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEQRQCPYPVLIAEDNALLRAVLEGNLREIGYDVVVAIDGKDALARIQSGYFPLVITD
CCCCCCCCCCCEEEECCHHHHHHHHHCCHHHCCCEEEEEECCHHHHHHHHCCCCCEEEHH
WVMPVMDGPELCRAVRSLGLEHYTYLILLTSRDSKESIISGLEAGADEYLVKPVTPEELT
HHHHCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCEEECCCCHHHHH
VRLMTARRIIDLESSLKQSMEEVRLLTMRDPLTGIYNRRYLEDRLHQEVKRTFRYERPIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VVMFDIDHFKRVNDTWGHLVGDQVLKACAESVRSGVRENIDWPVRYGGEEFVVVLPETDM
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCC
AGAVIVAERLRQRIALIQTSVGDGAVTVTASFGVASFTPPDQKEDLSIGEVLLERADRCL
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
YRAKGEGRNRVCSEQL
HHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDEQRQCPYPVLIAEDNALLRAVLEGNLREIGYDVVVAIDGKDALARIQSGYFPLVITD
CCCCCCCCCCEEEECCHHHHHHHHHCCHHHCCCEEEEEECCHHHHHHHHCCCCCEEEHH
WVMPVMDGPELCRAVRSLGLEHYTYLILLTSRDSKESIISGLEAGADEYLVKPVTPEELT
HHHHCCCCHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCEEECCCCHHHHH
VRLMTARRIIDLESSLKQSMEEVRLLTMRDPLTGIYNRRYLEDRLHQEVKRTFRYERPIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VVMFDIDHFKRVNDTWGHLVGDQVLKACAESVRSGVRENIDWPVRYGGEEFVVVLPETDM
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCC
AGAVIVAERLRQRIALIQTSVGDGAVTVTASFGVASFTPPDQKEDLSIGEVLLERADRCL
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
YRAKGEGRNRVCSEQL
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA